Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30397
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 38 0.003
At5g51440.1 68418.m06377 23.5 kDa mitochondrial small heat shock... 36 0.021
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 35 0.037
At4g25200.1 68417.m03627 23.6 kDa mitochondrial small heat shock... 33 0.086
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 33 0.086
At2g02160.1 68415.m00152 zinc finger (CCCH-type) family protein ... 33 0.15
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 33 0.15
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 33 0.15
At1g63230.1 68414.m07147 pentatricopeptide (PPR) repeat-containi... 32 0.26
At2g15630.1 68415.m01790 pentatricopeptide (PPR) repeat-containi... 31 0.46
At5g49580.1 68418.m06136 DNAJ heat shock N-terminal domain-conta... 29 1.9
At5g46850.1 68418.m05773 expressed protein ; expression support... 29 2.5
At2g41450.1 68415.m05121 GCN5-related N-acetyltransferase (GNAT)... 27 5.7
At5g60200.1 68418.m07546 Dof-type zinc finger domain-containing ... 27 7.5
At3g60280.1 68416.m06738 uclacyanin 3 (UCC3) identical to uclacy... 27 7.5
At1g73770.1 68414.m08541 hypothetical protein 27 7.5
At1g09690.1 68414.m01088 60S ribosomal protein L21 (RPL21C) Simi... 27 7.5
At1g09590.1 68414.m01076 60S ribosomal protein L21 (RPL21A) Simi... 27 7.5
At5g19020.1 68418.m02260 pentatricopeptide (PPR) repeat-containi... 27 9.9
At5g04750.1 68418.m00488 F1F0-ATPase inhibitor protein, putative... 27 9.9
At4g13700.1 68417.m02128 serine/threonine protein phosphatase fa... 27 9.9
At3g49060.1 68416.m05360 protein kinase family protein / U-box d... 27 9.9
At2g44430.1 68415.m05525 DNA-binding bromodomain-containing prot... 27 9.9
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 38.3 bits (85), Expect = 0.003
Identities = 33/111 (29%), Positives = 55/111 (49%), Gaps = 14/111 (12%)
Frame = +3
Query: 225 FRPWRASLARQESSSTINL------TKEKFEVILDVQQFTPDEITVKASNNTVV-VEG-K 380
F+ +S +E+S+ +N T E D+ +E+ V+ ++V+ + G +
Sbjct: 28 FKELTSSSLSRENSAIVNARVDWRETPEAHVFKADLPGLKKEEVKVEIEEDSVLKISGER 87
Query: 381 HEEKQDEHGFISR------QFTRRYILPTGYEVNDLVSTLSSDGVLTVTAP 515
H EK+D++ R QFTRR+ LP ++ D V +GVLTVT P
Sbjct: 88 HVEKEDKNDTWHRVERSSGQFTRRFRLPENVKM-DQVKAAMENGVLTVTVP 137
>At5g51440.1 68418.m06377 23.5 kDa mitochondrial small heat shock
protein (HSP23.5-M) similar to heat shock 22 kDa
protein, mitochondrial precursor SP:Q96331 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 210
Score = 35.5 bits (78), Expect = 0.021
Identities = 25/82 (30%), Positives = 41/82 (50%), Gaps = 5/82 (6%)
Frame = +3
Query: 285 KEKFEVI---LDVQQFTPDEITVKASNNTVVV--EGKHEEKQDEHGFISRQFTRRYILPT 449
KEK + + +D+ + +++ + NT+V+ EG+ EE +D G R+FT R LP
Sbjct: 114 KEKDDALHLRIDMPGLSREDVKLALEQNTLVIRGEGETEEGEDVSGD-GRRFTSRIELPE 172
Query: 450 GYEVNDLVSTLSSDGVLTVTAP 515
D + +GVL V P
Sbjct: 173 KVYKTDEIKAEMKNGVLKVVIP 194
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 34.7 bits (76), Expect = 0.037
Identities = 25/77 (32%), Positives = 42/77 (54%), Gaps = 8/77 (10%)
Frame = +3
Query: 309 DVQQFTPDEITVKASNNTVV-VEGKH----EEKQDEHGFISR---QFTRRYILPTGYEVN 464
D+ +E+ V+ +++V+ + G+ EEKQD + R QF+R++ LP ++
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKM- 122
Query: 465 DLVSTLSSDGVLTVTAP 515
D V +GVLTVT P
Sbjct: 123 DQVKASMENGVLTVTVP 139
>At4g25200.1 68417.m03627 23.6 kDa mitochondrial small heat shock
protein (HSP23.6-M) contains Pfam profile PF00011:
Hsp20/alpha crystallin family
Length = 210
Score = 33.5 bits (73), Expect = 0.086
Identities = 25/83 (30%), Positives = 42/83 (50%), Gaps = 6/83 (7%)
Frame = +3
Query: 285 KEKFEVI---LDVQQFTPDEITVKASNNTVVV--EGKHEEKQDEHGFI-SRQFTRRYILP 446
KEK + + +D+ + +++ + +T+V+ EGK+EE E G +R+FT R LP
Sbjct: 112 KEKDDALYLRIDMPGLSREDVKLALEQDTLVIRGEGKNEEDGGEEGESGNRRFTSRIGLP 171
Query: 447 TGYEVNDLVSTLSSDGVLTVTAP 515
D + +GVL V P
Sbjct: 172 DKIYKIDEIKAEMKNGVLKVVIP 194
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 33.5 bits (73), Expect = 0.086
Identities = 24/83 (28%), Positives = 44/83 (53%), Gaps = 8/83 (9%)
Frame = +3
Query: 282 TKEKFEVILDVQQFTPDEITVKASNNTVV-VEG----KHEEKQDEHGFISR---QFTRRY 437
T E E++LD+ DE+ ++ N V+ V G + E+K D+ + R +F R++
Sbjct: 78 TAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWRQF 137
Query: 438 ILPTGYEVNDLVSTLSSDGVLTV 506
LP ++ + + L +GVLT+
Sbjct: 138 KLPDNVDMESVKAKL-ENGVLTI 159
>At2g02160.1 68415.m00152 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 669
Score = 32.7 bits (71), Expect = 0.15
Identities = 20/73 (27%), Positives = 36/73 (49%), Gaps = 1/73 (1%)
Frame = +3
Query: 243 SLARQESSSTINL-TKEKFEVILDVQQFTPDEITVKASNNTVVVEGKHEEKQDEHGFISR 419
+++++E+ I L T+EK EV+ + + +E TV +V E +E +DE +
Sbjct: 552 TISKEEAGDEIKLITEEKTEVVSEPKAEVEEEGTVMEEEE-IVGEEVYEGNEDEQAYEGD 610
Query: 420 QFTRRYILPTGYE 458
+ Y GYE
Sbjct: 611 ELNGEYYYEEGYE 623
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 32.7 bits (71), Expect = 0.15
Identities = 24/77 (31%), Positives = 41/77 (53%), Gaps = 8/77 (10%)
Frame = +3
Query: 309 DVQQFTPDEITVKASNNTVV-VEGKH----EEKQDEHGFISRQ---FTRRYILPTGYEVN 464
D+ +E+ V+ +++V+ + G+ EEKQD + R F+R++ LP ++
Sbjct: 62 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGGFSRKFRLPENVKM- 120
Query: 465 DLVSTLSSDGVLTVTAP 515
D V +GVLTVT P
Sbjct: 121 DQVKASMENGVLTVTVP 137
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 32.7 bits (71), Expect = 0.15
Identities = 21/77 (27%), Positives = 44/77 (57%), Gaps = 8/77 (10%)
Frame = +3
Query: 309 DVQQFTPDEITVKASNNTVV-VEGK----HEEKQDEHGFISR---QFTRRYILPTGYEVN 464
D+ +E+ V+ + ++ + G+ +EEK D+ + R +FTRR+ LP ++
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 465 DLVSTLSSDGVLTVTAP 515
++ +++ +GVL+VT P
Sbjct: 126 EIKASM-ENGVLSVTVP 141
>At1g63230.1 68414.m07147 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 323
Score = 31.9 bits (69), Expect = 0.26
Identities = 18/53 (33%), Positives = 26/53 (49%)
Frame = +3
Query: 315 QQFTPDEITVKASNNTVVVEGKHEEKQDEHGFISRQFTRRYILPTGYEVNDLV 473
+Q PD +T A N +V EGK E ++ +G RR I PT N ++
Sbjct: 140 RQINPDVVTFSALINALVKEGKVSEAEEIYG----DMLRRGIFPTTITYNSMI 188
>At2g15630.1 68415.m01790 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 627
Score = 31.1 bits (67), Expect = 0.46
Identities = 19/63 (30%), Positives = 28/63 (44%)
Frame = +3
Query: 306 LDVQQFTPDEITVKASNNTVVVEGKHEEKQDEHGFISRQFTRRYILPTGYEVNDLVSTLS 485
+D+ PD++T + EGK EE ++ G + RR I P N L+S S
Sbjct: 493 MDMMSINPDDVTYNCLMRGLCGEGKFEEARELMG----EMKRRGIKPDHISYNTLISGYS 548
Query: 486 SDG 494
G
Sbjct: 549 KKG 551
>At5g49580.1 68418.m06136 DNAJ heat shock N-terminal
domain-containing protein contains similarity to S-locus
protein 5 GI:6069485 from [Brassica rapa]; contains Pfam
profile PF00226 DnaJ domain
Length = 695
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/43 (34%), Positives = 21/43 (48%), Gaps = 1/43 (2%)
Frame = +2
Query: 266 FDDQLDEREI*GYFRRSAIHSRRD-HGQGVEQHGCRRRQARGE 391
+DD+L E+ YFRR S++D G G G + GE
Sbjct: 471 YDDELKREELLNYFRRFQNSSQKDTRGHGFSGSGFGSSEGEGE 513
>At5g46850.1 68418.m05773 expressed protein ; expression supported
by MPSS
Length = 296
Score = 28.7 bits (61), Expect = 2.5
Identities = 16/62 (25%), Positives = 30/62 (48%), Gaps = 2/62 (3%)
Frame = +2
Query: 104 GAHRAPLQTTGPALRHGSEEGRPVILHQFIPLDLALQKFILQTLEGE--LSQTGIFFDDQ 277
G+HR + + L H + R +++ + +PL + F LQ+L+ S +F D
Sbjct: 99 GSHRNLITSIKLLLHHSESQFRELVIVERLPLGVFADPFELQSLQQRRAFSDVSVFGDTN 158
Query: 278 LD 283
L+
Sbjct: 159 LE 160
>At2g41450.1 68415.m05121 GCN5-related N-acetyltransferase (GNAT)
family protein low similarity to Swift [Xenopus laevis]
GI:14164561; contains Pfam profiles PF00583:
acetyltransferase, GNAT family, PF00533: BRCA1 C
Terminus (BRCT) domain
Length = 991
Score = 27.5 bits (58), Expect = 5.7
Identities = 15/40 (37%), Positives = 19/40 (47%)
Frame = +2
Query: 155 SEEGRPVILHQFIPLDLALQKFILQTLEGELSQTGIFFDD 274
S R +L F+PL LA F+LQ G +FDD
Sbjct: 580 SSYSRKFLLITFLPLSLACLAFLLQWRSGVNDSVTQWFDD 619
>At5g60200.1 68418.m07546 Dof-type zinc finger domain-containing
protein similar to dof6 zinc finger protein GI:5689615
from [Arabidopsis thaliana]
Length = 257
Score = 27.1 bits (57), Expect = 7.5
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +1
Query: 427 PAGTSCRPDTRSTTWSARCLPTACSPS 507
P G CR + RST+ +AR L T P+
Sbjct: 99 PVGGGCRKNKRSTSSAARSLRTTPEPA 125
>At3g60280.1 68416.m06738 uclacyanin 3 (UCC3) identical to
uclacyanin 3 GI:3395770 from [Arabidopsis thaliana];
contains Pfam profile PF02298: Plastocyanin-like domain;
identical to cDNA uclacyanin 3 (UCC3)GI:3395769
Length = 222
Score = 27.1 bits (57), Expect = 7.5
Identities = 16/36 (44%), Positives = 19/36 (52%)
Frame = +1
Query: 400 STASFPVSSPAGTSCRPDTRSTTWSARCLPTACSPS 507
ST S P S+P+ S P T ST S P+ SPS
Sbjct: 126 STPSSPPSTPSTPSSPPSTPSTPSSPPSPPSPPSPS 161
>At1g73770.1 68414.m08541 hypothetical protein
Length = 191
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +3
Query: 369 VEGKHEEKQDEHGFISRQFTRRYILPTGYEVNDLVSTLSSDG 494
+ GKH++ DE R+Y + ++V+D+ S + SDG
Sbjct: 97 LSGKHDDSDDE----VMDVVRKYPVNDAHKVDDIDSDIESDG 134
>At1g09690.1 68414.m01088 60S ribosomal protein L21 (RPL21C) Similar
to ribosomal protein L21 (gb|L38826). ESTs
gb|AA395597,gb|ATTS5197 come from this gene
Length = 164
Score = 27.1 bits (57), Expect = 7.5
Identities = 15/48 (31%), Positives = 22/48 (45%), Gaps = 1/48 (2%)
Frame = +3
Query: 330 DEITVKASNNTVVVEGKHEEKQDEHGFISRQFTRRYILPTGYE-VNDL 470
DE+ A N + K + K + GF+ T + P Y+ VNDL
Sbjct: 113 DELKAAAKANGETISTKRQPKGPKPGFMVEGMTLETVTPIPYDVVNDL 160
>At1g09590.1 68414.m01076 60S ribosomal protein L21 (RPL21A) Similar
to L21 family of ribosomal protein; amino acid sequence
is identical to F21M12.8
Length = 164
Score = 27.1 bits (57), Expect = 7.5
Identities = 15/48 (31%), Positives = 22/48 (45%), Gaps = 1/48 (2%)
Frame = +3
Query: 330 DEITVKASNNTVVVEGKHEEKQDEHGFISRQFTRRYILPTGYE-VNDL 470
DE+ A N + K + K + GF+ T + P Y+ VNDL
Sbjct: 113 DELKAAAKANGETISTKRQPKGPKPGFMVEGMTLETVTPIPYDVVNDL 160
>At5g19020.1 68418.m02260 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 939
Score = 26.6 bits (56), Expect = 9.9
Identities = 14/61 (22%), Positives = 27/61 (44%)
Frame = +3
Query: 234 WRASLARQESSSTINLTKEKFEVILDVQQFTPDEITVKASNNTVVVEGKHEEKQDEHGFI 413
W A ++ S + L F ++ Q PD IT+ + + + G EE + H ++
Sbjct: 659 WNAMISGYAQSLSPQLALHLFREMISSSQVKPDAITMVSVFSAISSLGSLEEGKRAHDYL 718
Query: 414 S 416
+
Sbjct: 719 N 719
>At5g04750.1 68418.m00488 F1F0-ATPase inhibitor protein, putative
similar to F1F0-ATPase inhibitor protein [Oryza sativa
(japonica cultivar-group)] gi|5106371|dbj|BAA81661
Length = 94
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/47 (31%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = +2
Query: 101 LGAHRAPLQTTGPALRHGSEE-GRPVILHQFIPLDLALQKFILQTLE 238
+GA R+ + T GPA+R+ S++ GR + + + +QK + LE
Sbjct: 22 IGASRSVVSTRGPAIRYFSDDKGRVLSEEERAKESMYIQKMERERLE 68
>At4g13700.1 68417.m02128 serine/threonine protein phosphatase
family protein contains Pfam domain PF00149: Ser/Thr
protein phosphatase
Length = 474
Score = 26.6 bits (56), Expect = 9.9
Identities = 9/29 (31%), Positives = 17/29 (58%)
Frame = +3
Query: 360 TVVVEGKHEEKQDEHGFISRQFTRRYILP 446
T+V+EG HE + G + ++ R+ +P
Sbjct: 278 TMVIEGNHEIEPQASGITFKSYSERFAVP 306
>At3g49060.1 68416.m05360 protein kinase family protein / U-box
domain-containing protein contains Pfam profile: PF00069
Eukaryotic protein kinase domain
Length = 805
Score = 26.6 bits (56), Expect = 9.9
Identities = 23/69 (33%), Positives = 31/69 (44%)
Frame = +2
Query: 197 LDLALQKFILQTLEGELSQTGIFFDDQLDEREI*GYFRRSAIHSRRDHGQGVEQHGCRRR 376
L L Q LQ LE E + FD + E+ FR+ R DH V++ RR
Sbjct: 378 LKLESQMRKLQDLEKEHGEK---FDTAM---ELLKSFRQKRDEIRIDHENAVKEVNALRR 431
Query: 377 QARGETGRA 403
+GETG +
Sbjct: 432 LVKGETGES 440
>At2g44430.1 68415.m05525 DNA-binding bromodomain-containing protein
contains Pfam domains, Pfam PF00439: Bromodomain and
PF00249: Myb-like DNA-binding domain
Length = 646
Score = 26.6 bits (56), Expect = 9.9
Identities = 13/58 (22%), Positives = 32/58 (55%), Gaps = 1/58 (1%)
Frame = +3
Query: 231 PWRASLARQESSSTINLTKEKFEVILDVQQFT-PDEITVKASNNTVVVEGKHEEKQDE 401
P +S ++++ + L++EK + V+ +++ +NNT +G++++KQ E
Sbjct: 462 PSSSSFSQKDDTKEETLSEEKDNIATGVRSSRRANKVAAVVANNTKTGKGRNKQKQTE 519
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,020,855
Number of Sequences: 28952
Number of extensions: 184759
Number of successful extensions: 650
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 634
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 649
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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