SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30395
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   316   1e-87
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...   178   3e-46
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...   166   1e-42
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...   125   5e-30
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...    99   2e-22
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...    95   6e-21
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...    89   5e-19
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    49   4e-07
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po...    36   0.005
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr...    31   0.10 
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce...    30   0.18 
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p...    26   2.9  
SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch...    25   6.7  
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo...    25   6.7  
SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    25   8.9  
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch...    25   8.9  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  316 bits (776), Expect = 1e-87
 Identities = 142/156 (91%), Positives = 152/156 (97%)
 Frame = +2

Query: 47  DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 226
           ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2   EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61

Query: 227 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 406
           RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ
Sbjct: 62  RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121

Query: 407 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGD 514
           I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGD
Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGD 157


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score =  178 bits (434), Expect = 3e-46
 Identities = 83/155 (53%), Positives = 110/155 (70%), Gaps = 1/155 (0%)
 Frame = +2

Query: 53  DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 232
           D   + +DNGSG  KAGFAGDD P+ +FP+ VGR +H+ VM    QKD +VG EAQ+ RG
Sbjct: 8   DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67

Query: 233 ILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREKMTQI 409
           +L ++ PIE GII NW DME+IW + + + +L   PEEHP+LLTE PL    N+EK+ + 
Sbjct: 68  LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127

Query: 410 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGD 514
            +ET N PA+  ++Q VL+LYAS RTTGIVL+ GD
Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTGIVLECGD 162


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score =  166 bits (404), Expect = 1e-42
 Identities = 78/154 (50%), Positives = 111/154 (72%), Gaps = 2/154 (1%)
 Frame = +2

Query: 59  AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 232
           A +V+DNG+G  K G+A D+ PR  FPSIVGRP  R +     +  KD  VGDEA++ R 
Sbjct: 4   APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63

Query: 233 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 412
           +L +KYP+E+GII ++++M ++W +TF+ +L++ P    +LLTE P+NP ANREKM + M
Sbjct: 64  LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123

Query: 413 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGD 514
           FE +    +YVAIQAVLSLYA G ++G+V+DSGD
Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGD 157


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score =  125 bits (301), Expect = 5e-30
 Identities = 74/182 (40%), Positives = 100/182 (54%), Gaps = 32/182 (17%)
 Frame = +2

Query: 65  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 193
           +++DNG+G  K G+AG+DAP  VFP+++   R  G   G         M  K        
Sbjct: 8   IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66

Query: 194 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 352
                 D ++G++A  K     +L YPI HG I NWD ME+ W  + +  LR  PE+H  
Sbjct: 67  RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126

Query: 353 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 508
           LLTE PLNP  NRE   +IMFE+FN   +Y+A+QAVL+L AS  +        TG V+DS
Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186

Query: 509 GD 514
           GD
Sbjct: 187 GD 188


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score =   99 bits (238), Expect = 2e-22
 Identities = 54/154 (35%), Positives = 84/154 (54%)
 Frame = +2

Query: 50  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 229
           D+V+A+V+D GS   + GF+G+D P+ V PS  G       + G    + Y+    +S  
Sbjct: 9   DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61

Query: 230 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 409
           G + +K  I +G + NWD    +W +    +L+  P EHP+L+TE   NP  NR K  + 
Sbjct: 62  G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120

Query: 410 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSG 511
           MFE+   PA Y+A Q   + +ASG+ T  ++D G
Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIG 154


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score = 95.1 bits (226), Expect = 6e-21
 Identities = 53/163 (32%), Positives = 96/163 (58%), Gaps = 3/163 (1%)
 Frame = +2

Query: 35  FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 214
           F+   +D+  LV+DNGS   +AG+ G+  P+ VF ++V R R +     + +  + VG++
Sbjct: 18  FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK----LSRTSTLVGND 72

Query: 215 AQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 391
              + G  ++ + P E  +I+NWD ME++  +TF  +L +   EHP+ +TE   NP   R
Sbjct: 73  TLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVR 131

Query: 392 EKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGD 514
             MT+++FE +N+P++   I  + S Y + +  ++GIVL+ G+
Sbjct: 132 STMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGN 174


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score = 88.6 bits (210), Expect = 5e-19
 Identities = 46/156 (29%), Positives = 87/156 (55%), Gaps = 2/156 (1%)
 Frame = +2

Query: 50  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 229
           +++ +LV+D GS   + G+AG+++P  + PS      + GV   +  ++ YV DE Q   
Sbjct: 8   EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61

Query: 230 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 403
            I  + +K    +GII +W+     W      +L+V P E+ +++TE   NP++ R+++ 
Sbjct: 62  PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121

Query: 404 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSG 511
           +  FE  + PA Y+  QAV   +A+ ++T +++D G
Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIG 157


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 49.2 bits (112), Expect = 4e-07
 Identities = 28/91 (30%), Positives = 44/91 (48%), Gaps = 4/91 (4%)
 Frame = +2

Query: 251 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 418
           PI+ G + +W+ ++  W H  Y+ L   P +    +PV L         +RE  TQ  FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171

Query: 419 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSG 511
               P   +A + ++ LYA G   G+V+D G
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIG 202


>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 401

 Score = 35.5 bits (78), Expect = 0.005
 Identities = 35/146 (23%), Positives = 60/146 (41%), Gaps = 4/146 (2%)
 Frame = +2

Query: 65  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 244
           +V+DNG+   KAGFAG        P+ + R +        G +  ++G+E  +     TL
Sbjct: 8   IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57

Query: 245 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 412
           ++   H  G + +W     +W     N   + P   ++ +LLT+      +      Q++
Sbjct: 58  QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117

Query: 413 FETFNSPAMYVAIQAVLSLYASGRTT 490
           FE F   A      A L  +  G  T
Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFT 143


>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1517

 Score = 31.1 bits (67), Expect = 0.10
 Identities = 16/48 (33%), Positives = 26/48 (54%)
 Frame = +2

Query: 110 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 253
           G+  PRA F  ++  P H G+++ M  KD   G+E  S +G  + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547


>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1666

 Score = 30.3 bits (65), Expect = 0.18
 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
 Frame = +2

Query: 311  FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 454
            +Y  L    E+HP+LLT+  A L P+ +  ++ +I  ++ N+P +    VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451


>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 26.2 bits (55), Expect = 2.9
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +2

Query: 353 LLTEAPLNPKANREKMTQIMFETFN 427
           +L  APL+ +   +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205


>SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter
           family|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 791

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
 Frame = -1

Query: 306 WCQIFS-MSSQLVMIPCSMGYFRVRIPLLL-WASSPT*ESFWPIPTITPWW 160
           WC+  S + SQ+  +   +G F    PL L WA   +  +    P ITPWW
Sbjct: 304 WCKPSSAVLSQIFGVKTGLGLF----PLTLDWAQISSLSN----PLITPWW 346


>SPBC1683.09c |frp1||ferric-chelate reductase
           Frp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 564

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 11/29 (37%), Positives = 17/29 (58%)
 Frame = +2

Query: 170 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 256
           +M+G     +Y+G  AQ KR +LT +  I
Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193


>SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 485

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 5/56 (8%)
 Frame = +2

Query: 77  NGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMG-----QKDSYVGDEAQSKR 229
           N +GM         + R  F S VG  +     VG       Q+DSY+ D++ S+R
Sbjct: 318 NATGMYGVSKGHSSSTRRPFFSDVGSSQPAEEFVGSSSSHGRQQDSYIADDSDSER 373


>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
           Y|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1002

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 14/35 (40%), Positives = 16/35 (45%)
 Frame = -3

Query: 274 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHH 170
           GDD+VLD V   +D    L L        AH D H
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFH 710


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,919,800
Number of Sequences: 5004
Number of extensions: 39420
Number of successful extensions: 142
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -