BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30389 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 106 2e-24 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 71 8e-14 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 70 2e-13 SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 60 3e-10 SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 36 0.004 SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 35 0.006 SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 34 0.011 SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 32 0.044 SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo... 32 0.059 SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom... 29 0.31 SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 28 0.72 SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccha... 28 0.95 SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 28 0.95 SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 27 1.3 SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase |Schizosac... 25 5.1 SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate syn... 25 5.1 SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 25 6.7 SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schiz... 25 6.7 SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual 25 8.9 SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 8.9 SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.9 SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces pombe... 25 8.9 SPBC582.10c |||ATP-dependent DNA helicase Rhp16b |Schizosaccharo... 25 8.9 SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|c... 25 8.9 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 106 bits (254), Expect = 2e-24 Identities = 47/144 (32%), Positives = 93/144 (64%) Frame = +1 Query: 64 EQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINF 243 ++++A R+AF +FD + G I ++ ++ ++GQ +ELQ +I+E D + +G I+F Sbjct: 8 DEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDF 67 Query: 244 DGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLD 423 F + + +++ D E +E++EAF+++D++GNGYIT L +L +L ++LS ++ Sbjct: 68 TEFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVA 124 Query: 424 GIIAEIDTDGSGTVDFDEFMEMMT 495 +I E DTDG G ++++EF +++ Sbjct: 125 DMIREADTDGDGVINYEEFSRVIS 148 Score = 26.6 bits (56), Expect = 2.2 Identities = 21/106 (19%), Positives = 43/106 (40%), Gaps = 1/106 (0%) Frame = +1 Query: 76 AMLRKAFQMFDTTKSGYIDVLKISTIL-NTMGQLFDDSELQALIDENDPENSGKINFDGF 252 A L+ D +G ID + T++ M ++ E++ D + +G I + Sbjct: 48 AELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEEL 107 Query: 253 CNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAA 390 +H L Q+E+ + R D +G+G I ++++ Sbjct: 108 ----THVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVISS 149 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 71.3 bits (167), Expect = 8e-14 Identities = 35/143 (24%), Positives = 74/143 (51%) Frame = +1 Query: 64 EQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINF 243 E++ + +AF++FD+ K ID ++ + +G + SE+ ++ + D G + Sbjct: 33 EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQM 92 Query: 244 DGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLD 423 + F + + + E D +E+K AF L+D + G I+ L+ + L++ + + +L+ Sbjct: 93 EDFVRVMTEKIVERDP---LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149 Query: 424 GIIAEIDTDGSGTVDFDEFMEMM 492 +I E D D G ++ EF+ +M Sbjct: 150 AMIEEFDLDQDGEINEQEFIAIM 172 Score = 42.3 bits (95), Expect = 4e-05 Identities = 20/74 (27%), Positives = 39/74 (52%) Frame = +1 Query: 274 LEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDG 453 L E E +Q++ EAF+L+D + + I L+ + AL +++ I+ + D G Sbjct: 27 LRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTG 86 Query: 454 SGTVDFDEFMEMMT 495 G + ++F+ +MT Sbjct: 87 KGYLQMEDFVRVMT 100 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 69.7 bits (163), Expect = 2e-13 Identities = 42/146 (28%), Positives = 80/146 (54%) Frame = +1 Query: 49 MDSDDEQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENS 228 M + EQ M ++AF ++D K G I + ++L ++G D+EL L +E Sbjct: 1 MPASKEQTDEM-KEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----G 55 Query: 229 GKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLS 408 I+ F + S+ L E ++E +E +AFR++D++ +GYI T+ + + L +KLS Sbjct: 56 DAIDEKKFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLS 112 Query: 409 NADLDGIIAEIDTDGSGTVDFDEFME 486 + ++ ++ E D SG+ D+ +F++ Sbjct: 113 DNEVQLMVQEADPTNSGSFDYYDFVQ 138 >SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 174 Score = 59.7 bits (138), Expect = 3e-10 Identities = 44/154 (28%), Positives = 78/154 (50%), Gaps = 6/154 (3%) Frame = +1 Query: 55 SDDEQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGK 234 S +++ +RK F D +SG ID + +I + S L +++DE + G Sbjct: 17 SFSNEEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA-SRLFSVVDE---DGGGD 72 Query: 235 INFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAAL------D 396 ++F F N S F + E ++LK AF++YD + +GYI+ L +L + + Sbjct: 73 VDFQEFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLRE 129 Query: 397 DKLSNADLDGIIAEIDTDGSGTVDFDEFMEMMTG 498 D+L +D I E+D D G + F+EF ++++G Sbjct: 130 DQLQQI-VDKTIMEVDKDRDGKISFEEFKDIVSG 162 >SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 35.9 bits (79), Expect = 0.004 Identities = 17/47 (36%), Positives = 28/47 (59%) Frame = +1 Query: 304 QELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEID 444 QELKEAF L D++G+G I +K +L +L+ S ++ + I+ Sbjct: 48 QELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 35.1 bits (77), Expect = 0.006 Identities = 18/60 (30%), Positives = 35/60 (58%) Frame = +1 Query: 313 KEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 492 K+AF L+DR G G I +++ ++L A + A+ I EI++ VD ++F++++ Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAE----ITEIESTLPAEVDMEQFLQVL 64 >SPAC1687.14c |||EF hand family protein, unknown role|Schizosaccharomyces pombe|chr 1|||Manual Length = 76 Score = 34.3 bits (75), Expect = 0.011 Identities = 21/64 (32%), Positives = 32/64 (50%) Frame = +1 Query: 298 MQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDE 477 M +E +EAF L+D GYI L+ A L + L+ L ++ T+G V +E Sbjct: 10 MDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGK--VSREE 67 Query: 478 FMEM 489 F E+ Sbjct: 68 FAEL 71 Score = 32.7 bits (71), Expect = 0.033 Identities = 17/61 (27%), Positives = 30/61 (49%) Frame = +1 Query: 88 KAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNIAS 267 +AF +FD T GYID + +G+ +LQ ++D +GK++ + F + Sbjct: 16 EAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDL--AGTNGKVSREEFAELWI 73 Query: 268 H 270 H Sbjct: 74 H 74 >SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 32.3 bits (70), Expect = 0.044 Identities = 15/33 (45%), Positives = 19/33 (57%) Frame = +1 Query: 280 EEDAEAMQQELKEAFRLYDREGNGYITTSTLKE 378 ++D M Q LKE Y+ E N IT+ TLKE Sbjct: 522 KDDLTEMNQRLKEQIESYENEVNSEITSRTLKE 554 >SPBC11G11.02c |end3||actin cortical patch component End3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 31.9 bits (69), Expect = 0.059 Identities = 22/55 (40%), Positives = 29/55 (52%) Frame = +1 Query: 316 EAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEF 480 E FR + E NGY++ S +L + KLS+ L+ I D D G DFDEF Sbjct: 11 EIFRGLNPE-NGYLSGSKAAGVLRS--SKLSSDKLEKIWDLADIDDDGMFDFDEF 62 >SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 441 Score = 29.5 bits (63), Expect = 0.31 Identities = 17/46 (36%), Positives = 27/46 (58%) Frame = +1 Query: 136 LKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNIASHF 273 + +ST+ +T + DDS L+ +ID+ PE GK NI++HF Sbjct: 12 ISVSTVEDTSFEAMDDSILE-IIDDEAPEIQGKPK----ANISTHF 52 >SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 190 Score = 28.3 bits (60), Expect = 0.72 Identities = 27/112 (24%), Positives = 49/112 (43%), Gaps = 1/112 (0%) Frame = +1 Query: 61 DEQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSEL-QALIDENDPENSGKI 237 D++++ K F F SG+++ + I D S + + + D + +G I Sbjct: 23 DKKELQQWYKGF--FKDCPSGHLNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYI 80 Query: 238 NFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAAL 393 +F F S E + +L AF+LYD + NG I+ + I+ A+ Sbjct: 81 DFKEFICALSVTSRGE----LNDKLIWAFQLYDLDNNGLISYDEMLRIVDAI 128 >SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccharomyces pombe|chr 2|||Manual Length = 514 Score = 27.9 bits (59), Expect = 0.95 Identities = 19/81 (23%), Positives = 40/81 (49%), Gaps = 3/81 (3%) Frame = +1 Query: 130 DVLKISTILNTM-GQLFDDSELQALIDENDP--ENSGKINFDGFCNIASHFLEEEDAEAM 300 + ++I+ L + G+ D ++ + + +P ENS N D +A+ FL+E + E Sbjct: 402 ETIRIAVNLQELIGKYLGDKDIFQIENFINPIEENSADTNDDIVNQVAAQFLDEREFETD 461 Query: 301 QQELKEAFRLYDREGNGYITT 363 ++E + + L ++ I T Sbjct: 462 EEEEESQYLLSTKDAINAINT 482 >SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccharomyces pombe|chr 2|||Manual Length = 884 Score = 27.9 bits (59), Expect = 0.95 Identities = 29/116 (25%), Positives = 44/116 (37%) Frame = +1 Query: 145 STILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAF 324 ST + + ++ D+ Q+ +D +S D NI + E+ + Q F Sbjct: 256 STKSSKLDEISADNNAQSAVDNK--YSSPPQTIDPQANIKALITPEDRSNFYQ-----LF 308 Query: 325 RLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 492 D E GYI A L + +L I +DT G +D DEF M Sbjct: 309 SKIDNENKGYIVGGEAVPFFMA--SHLDSEELARIWDTVDTQDRGYIDKDEFAVAM 362 >SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 418 Score = 27.5 bits (58), Expect = 1.3 Identities = 15/68 (22%), Positives = 29/68 (42%) Frame = +1 Query: 202 IDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEI 381 + E + F F + ++ +D A+ Q + +F +R G G I T ++ Sbjct: 343 VSEESQVEPNTLIFQNFSSPSTSLSAPKDTPAVIQSINTSFLNNERAGRGNIKTVDQNQL 402 Query: 382 LAALDDKL 405 + + DKL Sbjct: 403 INRIVDKL 410 >SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase |Schizosaccharomyces pombe|chr 2|||Manual Length = 205 Score = 25.4 bits (53), Expect = 5.1 Identities = 14/36 (38%), Positives = 20/36 (55%) Frame = +1 Query: 337 REGNGYITTSTLKEILAALDDKLSNADLDGIIAEID 444 RE GY+ T + D L+NA+L I+A+ID Sbjct: 107 REETGYVGTVMDSTTVMYNDPGLTNANLKIILADID 142 >SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate synthase Lsp1|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 25.4 bits (53), Expect = 5.1 Identities = 20/100 (20%), Positives = 41/100 (41%), Gaps = 5/100 (5%) Frame = +1 Query: 202 IDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQ---ELKEAFRLYDREGNGYITTSTL 372 I +N P + IN + ++ EE E KE LY+++ G++ + Sbjct: 181 IMKNSPMPAEPINKENINQLSRSLFYEEGKEFWDDYDIAAKEILSLYEQDPEGFVNPYSK 240 Query: 373 KEILAALDDKLSNADLDGIIAEI--DTDGSGTVDFDEFME 486 + A + + + G+ A+ D D + +EF++ Sbjct: 241 RGAAALANSENNKGSEAGVSAKTNPDMDSDSSAIVNEFLK 280 >SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1183 Score = 25.0 bits (52), Expect = 6.7 Identities = 11/36 (30%), Positives = 21/36 (58%), Gaps = 1/36 (2%) Frame = +1 Query: 328 LYDREGNGYITT-STLKEILAALDDKLSNADLDGII 432 LYD+E GY+T + E++ + D+ N D + ++ Sbjct: 1055 LYDKEKRGYVTLFPSGSEVVQFVPDRTYNIDSEYVV 1090 >SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.0 bits (52), Expect = 6.7 Identities = 9/21 (42%), Positives = 14/21 (66%) Frame = +1 Query: 238 NFDGFCNIASHFLEEEDAEAM 300 + DGF ++ASHF AE++ Sbjct: 269 SIDGFVSVASHFSSASTAESL 289 >SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual Length = 533 Score = 24.6 bits (51), Expect = 8.9 Identities = 15/40 (37%), Positives = 20/40 (50%) Frame = +1 Query: 208 ENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFR 327 E D EN ++ A LE+ED EAM +L+E R Sbjct: 152 EIDKENLAVLSQKSVLKTAEE-LEKEDREAMSAKLQELIR 190 >SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3131 Score = 24.6 bits (51), Expect = 8.9 Identities = 14/45 (31%), Positives = 21/45 (46%) Frame = +1 Query: 205 DENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDR 339 D N+PE+ GF +I S +E D E+ + +YDR Sbjct: 645 DCNNPESFSLFVDAGFISITSQNVETNDKESNSESETLYHLIYDR 689 >SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1379 Score = 24.6 bits (51), Expect = 8.9 Identities = 9/16 (56%), Positives = 14/16 (87%) Frame = -2 Query: 488 ISMNSSKSTVPEPSVS 441 +S+ SS +T+P+PSVS Sbjct: 440 LSLKSSDATIPKPSVS 455 >SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces pombe|chr 1|||Manual Length = 504 Score = 24.6 bits (51), Expect = 8.9 Identities = 10/27 (37%), Positives = 18/27 (66%) Frame = +1 Query: 109 TTKSGYIDVLKISTILNTMGQLFDDSE 189 TTKS +I++ ST + ++F+DS+ Sbjct: 143 TTKSRFIEISATSTTVADCRKIFEDSQ 169 >SPBC582.10c |||ATP-dependent DNA helicase Rhp16b |Schizosaccharomyces pombe|chr 2|||Manual Length = 830 Score = 24.6 bits (51), Expect = 8.9 Identities = 17/48 (35%), Positives = 26/48 (54%) Frame = -1 Query: 144 DLQHVDVTRLRGVKHLECFTQHGHLLFIIGIHVARQGLAAVTRTEXRM 1 D Q D T +RG++ F+Q L II +H+ + G+ V R + RM Sbjct: 659 DEQEEDDT-VRGLRKTIIFSQFTTFLDIIDLHLRKAGIGFV-RYDGRM 704 >SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|chr 3|||Manual Length = 577 Score = 24.6 bits (51), Expect = 8.9 Identities = 15/50 (30%), Positives = 23/50 (46%) Frame = +2 Query: 155 STQWDSCSMTQNFKHSSMKTIRKTVGRSTSMASVILLPISLKRKTLKPCN 304 S Q D + + +K + I G S ++ + LP SL +KPCN Sbjct: 356 SYQSDLVAAIKKYKINLRSLIIIDEGVSNTVKDLAFLPQSLTTLIVKPCN 405 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,976,033 Number of Sequences: 5004 Number of extensions: 38001 Number of successful extensions: 163 Number of sequences better than 10.0: 24 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 155 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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