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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30380
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    87   1e-18
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    46   3e-06
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    46   3e-06
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce...    44   1e-05
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    34   0.011
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos...    31   0.10 
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi...    27   1.3  
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    26   3.8  
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma...    25   6.7  
SPAC644.12 |cdc5||cell division control protein Cdc5|Schizosacch...    25   8.9  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 87.0 bits (206), Expect = 1e-18
 Identities = 44/135 (32%), Positives = 76/135 (56%)
 Frame = +1

Query: 67  RIAVLRKAFFGFDHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGRLEFPE 246
           +IA  R+AF  FD ++ G+I  + +  ++R +G       L+++I EVDAD +G ++F E
Sbjct: 10  QIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTE 69

Query: 247 FVTLAAKFIVEEDAEAXXXELREAFRLYDKEGNGXXPTSSLREIXXXXXXXXXXXXXXXX 426
           F+T+ A+ + + D E    E+REAF+++DK+GNG      L  +                
Sbjct: 70  FLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADM 126

Query: 427 IQEIDTDGSGTVDFD 471
           I+E DTDG G ++++
Sbjct: 127 IREADTDGDGVINYE 141


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 46.0 bits (104), Expect = 3e-06
 Identities = 30/130 (23%), Positives = 59/130 (45%)
 Frame = +1

Query: 79  LRKAFFGFDHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGRLEFPEFVTL 258
           +++AF  +D ++ G IP   V  +LR +G       L +L  E+       ++  +F++ 
Sbjct: 11  MKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GDAIDEKKFMSF 66

Query: 259 AAKFIVEEDAEAXXXELREAFRLYDKEGNGXXPTSSLREIXXXXXXXXXXXXXXXXIQEI 438
            +  + E ++E    E  +AFR++DK+ +G   T+   +                 +QE 
Sbjct: 67  VSNKLRETESEE---EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA 123

Query: 439 DTDGSGTVDF 468
           D   SG+ D+
Sbjct: 124 DPTNSGSFDY 133



 Score = 27.1 bits (57), Expect = 1.7
 Identities = 11/25 (44%), Positives = 17/25 (68%)
 Frame = +1

Query: 304 ELREAFRLYDKEGNGXXPTSSLREI 378
           E++EAF LYD + +G  PTS +  +
Sbjct: 10  EMKEAFVLYDIDKDGLIPTSHVGSV 34


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 46.0 bits (104), Expect = 3e-06
 Identities = 29/129 (22%), Positives = 55/129 (42%)
 Frame = +1

Query: 79  LRKAFFGFDHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGRLEFPEFVTL 258
           + +AF  FD ++  +I    +   +R +G       + +++ + D    G L+  +FV +
Sbjct: 39  INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRV 98

Query: 259 AAKFIVEEDAEAXXXELREAFRLYDKEGNGXXPTSSLREIXXXXXXXXXXXXXXXXIQEI 438
             + IVE D      E++ AF L+D +  G     +LR +                I+E 
Sbjct: 99  MTEKIVERD---PLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF 155

Query: 439 DTDGSGTVD 465
           D D  G ++
Sbjct: 156 DLDQDGEIN 164



 Score = 34.3 bits (75), Expect = 0.011
 Identities = 16/60 (26%), Positives = 31/60 (51%)
 Frame = +1

Query: 79  LRKAFFGFDHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGRLEFPEFVTL 258
           +++AF  FD + +G I    +  + + +    + + LE +IEE D D  G +   EF+ +
Sbjct: 112 IKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAI 171


>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 174

 Score = 44.0 bits (99), Expect = 1e-05
 Identities = 37/145 (25%), Positives = 58/145 (40%), Gaps = 5/145 (3%)
 Frame = +1

Query: 52  SCPHNRIAVLRKAFFGFDHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGR 231
           S  +  I  +RK F   D N+SGSI  +    I  +   P    +   L   VD D  G 
Sbjct: 17  SFSNEEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNP----LASRLFSVVDEDGGGD 72

Query: 232 LEFPEFVTLAAKFIVEEDAEAXXXELREAFRLYDKEGNGXXPTSSL-----REIXXXXXX 396
           ++F EF+   + F V  + E    +L+ AF++YD + +G      L       +      
Sbjct: 73  VDFQEFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLRE 129

Query: 397 XXXXXXXXXXIQEIDTDGSGTVDFD 471
                     I E+D D  G + F+
Sbjct: 130 DQLQQIVDKTIMEVDKDRDGKISFE 154


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 34.3 bits (75), Expect = 0.011
 Identities = 24/99 (24%), Positives = 46/99 (46%)
 Frame = +1

Query: 82  RKAFFGFDHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGRLEFPEFVTLA 261
           ++AF  FD + +G IP   + D+LR  G   N  + E  I E+++     ++  +F+ + 
Sbjct: 9   KQAFSLFDRHGTGRIPKTSIGDLLRACGQ--NPTLAE--ITEIESTLPAEVDMEQFLQVL 64

Query: 262 AKFIVEEDAEAXXXELREAFRLYDKEGNGXXPTSSLREI 378
            +     D      E  + F+++DK+  G      LR +
Sbjct: 65  NR-PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYV 102


>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
           Plc1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 899

 Score = 31.1 bits (67), Expect = 0.10
 Identities = 15/49 (30%), Positives = 26/49 (53%)
 Frame = +1

Query: 103 DHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGRLEFPEF 249
           +  +S  +    V  + +M+    + + LEE  ++ DAD SG+L F EF
Sbjct: 307 EKEQSAQLDLGDVHRMCQMLHLNASMEFLEETFQKADADHSGKLSFEEF 355


>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
           Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 18/53 (33%), Positives = 27/53 (50%)
 Frame = +1

Query: 190 EELIEEVDADXSGRLEFPEFVTLAAKFIVEEDAEAXXXELREAFRLYDKEGNG 348
           E +    DAD +G ++F EF+   +   V    E    +L  AF+LYD + NG
Sbjct: 66  EYVFNVFDADKNGYIDFKEFICALS---VTSRGE-LNDKLIWAFQLYDLDNNG 114


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 10/15 (66%), Positives = 13/15 (86%)
 Frame = +1

Query: 304 ELREAFRLYDKEGNG 348
           EL+EAF L DK+G+G
Sbjct: 49  ELKEAFALLDKDGDG 63


>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 614

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 11/22 (50%), Positives = 15/22 (68%)
 Frame = +1

Query: 187 LEELIEEVDADXSGRLEFPEFV 252
           + E I EV+ D SGR+E  +FV
Sbjct: 56  VREAIREVNVDSSGRVEPEDFV 77


>SPAC644.12 |cdc5||cell division control protein
           Cdc5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 757

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 15/51 (29%), Positives = 23/51 (45%)
 Frame = -2

Query: 158 ILRISATKSQGILPERLWSXPKKALRRTAILLWGQLIGPVFGVPEPWLMSL 6
           +L   AT  +  + ER+    + A  R + ++   LI P    PE W  SL
Sbjct: 500 VLEEDATDRERRIQERIAEKERLAKARRSQVIQRDLIRPSVTQPEKWKRSL 550


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,441,172
Number of Sequences: 5004
Number of extensions: 20910
Number of successful extensions: 80
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 71
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 74
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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