BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30380 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 87 1e-18 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 46 3e-06 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 46 3e-06 SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 44 1e-05 SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 34 0.011 SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 31 0.10 SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 27 1.3 SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 26 3.8 SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 25 6.7 SPAC644.12 |cdc5||cell division control protein Cdc5|Schizosacch... 25 8.9 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 87.0 bits (206), Expect = 1e-18 Identities = 44/135 (32%), Positives = 76/135 (56%) Frame = +1 Query: 67 RIAVLRKAFFGFDHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGRLEFPE 246 +IA R+AF FD ++ G+I + + ++R +G L+++I EVDAD +G ++F E Sbjct: 10 QIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTE 69 Query: 247 FVTLAAKFIVEEDAEAXXXELREAFRLYDKEGNGXXPTSSLREIXXXXXXXXXXXXXXXX 426 F+T+ A+ + + D E E+REAF+++DK+GNG L + Sbjct: 70 FLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADM 126 Query: 427 IQEIDTDGSGTVDFD 471 I+E DTDG G ++++ Sbjct: 127 IREADTDGDGVINYE 141 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 46.0 bits (104), Expect = 3e-06 Identities = 30/130 (23%), Positives = 59/130 (45%) Frame = +1 Query: 79 LRKAFFGFDHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGRLEFPEFVTL 258 +++AF +D ++ G IP V +LR +G L +L E+ ++ +F++ Sbjct: 11 MKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GDAIDEKKFMSF 66 Query: 259 AAKFIVEEDAEAXXXELREAFRLYDKEGNGXXPTSSLREIXXXXXXXXXXXXXXXXIQEI 438 + + E ++E E +AFR++DK+ +G T+ + +QE Sbjct: 67 VSNKLRETESEE---EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA 123 Query: 439 DTDGSGTVDF 468 D SG+ D+ Sbjct: 124 DPTNSGSFDY 133 Score = 27.1 bits (57), Expect = 1.7 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = +1 Query: 304 ELREAFRLYDKEGNGXXPTSSLREI 378 E++EAF LYD + +G PTS + + Sbjct: 10 EMKEAFVLYDIDKDGLIPTSHVGSV 34 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 46.0 bits (104), Expect = 3e-06 Identities = 29/129 (22%), Positives = 55/129 (42%) Frame = +1 Query: 79 LRKAFFGFDHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGRLEFPEFVTL 258 + +AF FD ++ +I + +R +G + +++ + D G L+ +FV + Sbjct: 39 INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRV 98 Query: 259 AAKFIVEEDAEAXXXELREAFRLYDKEGNGXXPTSSLREIXXXXXXXXXXXXXXXXIQEI 438 + IVE D E++ AF L+D + G +LR + I+E Sbjct: 99 MTEKIVERD---PLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF 155 Query: 439 DTDGSGTVD 465 D D G ++ Sbjct: 156 DLDQDGEIN 164 Score = 34.3 bits (75), Expect = 0.011 Identities = 16/60 (26%), Positives = 31/60 (51%) Frame = +1 Query: 79 LRKAFFGFDHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGRLEFPEFVTL 258 +++AF FD + +G I + + + + + + LE +IEE D D G + EF+ + Sbjct: 112 IKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAI 171 >SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 174 Score = 44.0 bits (99), Expect = 1e-05 Identities = 37/145 (25%), Positives = 58/145 (40%), Gaps = 5/145 (3%) Frame = +1 Query: 52 SCPHNRIAVLRKAFFGFDHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGR 231 S + I +RK F D N+SGSI + I + P + L VD D G Sbjct: 17 SFSNEEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNP----LASRLFSVVDEDGGGD 72 Query: 232 LEFPEFVTLAAKFIVEEDAEAXXXELREAFRLYDKEGNGXXPTSSL-----REIXXXXXX 396 ++F EF+ + F V + E +L+ AF++YD + +G L + Sbjct: 73 VDFQEFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLRE 129 Query: 397 XXXXXXXXXXIQEIDTDGSGTVDFD 471 I E+D D G + F+ Sbjct: 130 DQLQQIVDKTIMEVDKDRDGKISFE 154 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 34.3 bits (75), Expect = 0.011 Identities = 24/99 (24%), Positives = 46/99 (46%) Frame = +1 Query: 82 RKAFFGFDHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGRLEFPEFVTLA 261 ++AF FD + +G IP + D+LR G N + E I E+++ ++ +F+ + Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDLLRACGQ--NPTLAE--ITEIESTLPAEVDMEQFLQVL 64 Query: 262 AKFIVEEDAEAXXXELREAFRLYDKEGNGXXPTSSLREI 378 + D E + F+++DK+ G LR + Sbjct: 65 NR-PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYV 102 >SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 899 Score = 31.1 bits (67), Expect = 0.10 Identities = 15/49 (30%), Positives = 26/49 (53%) Frame = +1 Query: 103 DHNRSGSIPCDFVADILRMMGXPFNXKILEELIEEVDADXSGRLEFPEF 249 + +S + V + +M+ + + LEE ++ DAD SG+L F EF Sbjct: 307 EKEQSAQLDLGDVHRMCQMLHLNASMEFLEETFQKADADHSGKLSFEEF 355 >SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 190 Score = 27.5 bits (58), Expect = 1.3 Identities = 18/53 (33%), Positives = 27/53 (50%) Frame = +1 Query: 190 EELIEEVDADXSGRLEFPEFVTLAAKFIVEEDAEAXXXELREAFRLYDKEGNG 348 E + DAD +G ++F EF+ + V E +L AF+LYD + NG Sbjct: 66 EYVFNVFDADKNGYIDFKEFICALS---VTSRGE-LNDKLIWAFQLYDLDNNG 114 >SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 25.8 bits (54), Expect = 3.8 Identities = 10/15 (66%), Positives = 13/15 (86%) Frame = +1 Query: 304 ELREAFRLYDKEGNG 348 EL+EAF L DK+G+G Sbjct: 49 ELKEAFALLDKDGDG 63 >SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Manual Length = 614 Score = 25.0 bits (52), Expect = 6.7 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = +1 Query: 187 LEELIEEVDADXSGRLEFPEFV 252 + E I EV+ D SGR+E +FV Sbjct: 56 VREAIREVNVDSSGRVEPEDFV 77 >SPAC644.12 |cdc5||cell division control protein Cdc5|Schizosaccharomyces pombe|chr 1|||Manual Length = 757 Score = 24.6 bits (51), Expect = 8.9 Identities = 15/51 (29%), Positives = 23/51 (45%) Frame = -2 Query: 158 ILRISATKSQGILPERLWSXPKKALRRTAILLWGQLIGPVFGVPEPWLMSL 6 +L AT + + ER+ + A R + ++ LI P PE W SL Sbjct: 500 VLEEDATDRERRIQERIAEKERLAKARRSQVIQRDLIRPSVTQPEKWKRSL 550 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,441,172 Number of Sequences: 5004 Number of extensions: 20910 Number of successful extensions: 80 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 71 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 74 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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