Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30359
         (516 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q12874 Cluster: Splicing factor 3A subunit 3; n=31; Bil...    92   6e-18
UniRef50_Q6TUF1 Cluster: LRRGT00093; n=3; Eutheria|Rep: LRRGT000...    89   7e-17
UniRef50_A7T279 Cluster: Predicted protein; n=1; Nematostella ve...    73   4e-12
UniRef50_Q16EE4 Cluster: Splicing factor 3a; n=2; Culicidae|Rep:...    59   5e-08
UniRef50_Q22469 Cluster: Putative uncharacterized protein; n=5; ...    58   9e-08
UniRef50_Q9FG01 Cluster: Splicing factor 3a; n=6; Magnoliophyta|...    44   0.002
UniRef50_Q55CK7 Cluster: C2H2 type Zn finger-containing protein;...    42   0.011
UniRef50_A4QU30 Cluster: Putative uncharacterized protein; n=1; ...    41   0.019
UniRef50_O59706 Cluster: U2 snRNP-associated protein sap61; n=1;...    39   0.078
UniRef50_Q54UG9 Cluster: C2H2 type Zn finger-containing protein;...    33   3.9  
UniRef50_UPI00006CFDC8 Cluster: splicesome-associated protein, p...    33   5.1  
UniRef50_UPI00006CB7B0 Cluster: EF hand family protein; n=1; Tet...    32   8.9  

>UniRef50_Q12874 Cluster: Splicing factor 3A subunit 3; n=31;
           Bilateria|Rep: Splicing factor 3A subunit 3 - Homo
           sapiens (Human)
          Length = 501

 Score = 92.3 bits (219), Expect = 6e-18
 Identities = 42/64 (65%), Positives = 51/64 (79%)
 Frame = +3

Query: 186 RIYMDRYIEASIRLKELXEDKDGLRKEEISALLGXHEXQEFYSRLKQIKEFXRKHPNEIS 365
           R   DRY+E S  L++L +DKDGLRKEE++A+ G +E  EFY+RLKQIKEF RKHPNEI 
Sbjct: 44  RAMQDRYMEVSGNLRDLYDDKDGLRKEELNAISGPNEFAEFYNRLKQIKEFHRKHPNEIC 103

Query: 366 VPMS 377
           VPMS
Sbjct: 104 VPMS 107



 Score = 50.8 bits (116), Expect = 2e-05
 Identities = 46/149 (30%), Positives = 63/149 (42%), Gaps = 1/149 (0%)
 Frame = +1

Query: 73  QQRSYHEERERTMDAMVKEILHKKTGHXGDYKC*SSFEEFTWID-ILKPL*DLKNXMKIK 249
           QQR YHEE+ER MD M KE+L KK+         S        D  ++   +L++    K
Sbjct: 7   QQRRYHEEKERLMDVMAKEMLTKKSTLRDQIN--SDHRTRAMQDRYMEVSGNLRDLYDDK 64

Query: 250 MDYEKKKYQHC*VHMSXRSSIRDLSKLKSXIENIQMKYLYPCPVEFEEVANXRGKSLLRI 429
               K++                L ++K        +   P  VEFEE+   R ++    
Sbjct: 65  DGLRKEELNAISGPNEFAEFYNRLKQIKEFHRKHPNEICVPMSVEFEELLKAR-ENPSEE 123

Query: 430 TQFAXEXTDEEGYGKYFGPTIARYEKYIN 516
            Q   E TDEEGYG+Y       Y KYIN
Sbjct: 124 AQNLVEFTDEEGYGRYLDLHDC-YLKYIN 151


>UniRef50_Q6TUF1 Cluster: LRRGT00093; n=3; Eutheria|Rep: LRRGT00093
           - Rattus norvegicus (Rat)
          Length = 230

 Score = 88.6 bits (210), Expect = 7e-17
 Identities = 40/59 (67%), Positives = 49/59 (83%)
 Frame = +3

Query: 201 RYIEASIRLKELXEDKDGLRKEEISALLGXHEXQEFYSRLKQIKEFXRKHPNEISVPMS 377
           RY+E S  L++L +DKDGLRKEE++A+ G +E  EFY+RLKQIKEF RKHPNEI VPMS
Sbjct: 33  RYMEVSGNLRDLYDDKDGLRKEELNAISGPNEFAEFYNRLKQIKEFHRKHPNEICVPMS 91



 Score = 47.6 bits (108), Expect = 2e-04
 Identities = 47/148 (31%), Positives = 66/148 (44%)
 Frame = +1

Query: 73  QQRSYHEERERTMDAMVKEILHKKTGHXGDYKC*SSFEEFTWIDILKPL*DLKNXMKIKM 252
           QQR YHEE+ER MD M KE+L KK+         + + E +    L+ L D K+ ++   
Sbjct: 7   QQRRYHEEKERLMDVMAKEMLTKKS---------TRYMEVS--GNLRDLYDDKDGLR--- 52

Query: 253 DYEKKKYQHC*VHMSXRSSIRDLSKLKSXIENIQMKYLYPCPVEFEEVANXRGKSLLRIT 432
              K++                L ++K        +   P  VEFEE+   R ++     
Sbjct: 53  ---KEELNAISGPNEFAEFYNRLKQIKEFHRKHPNEICVPMSVEFEELLKAR-ENPSEEA 108

Query: 433 QFAXEXTDEEGYGKYFGPTIARYEKYIN 516
           Q   E TDEEGYG+Y       Y KYIN
Sbjct: 109 QNLVEFTDEEGYGRYLDLHDC-YLKYIN 135


>UniRef50_A7T279 Cluster: Predicted protein; n=1; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 365

 Score = 72.9 bits (171), Expect = 4e-12
 Identities = 32/63 (50%), Positives = 47/63 (74%)
 Frame = +3

Query: 186 RIYMDRYIEASIRLKELXEDKDGLRKEEISALLGXHEXQEFYSRLKQIKEFXRKHPNEIS 365
           R  ++R + A+  +  L ED+DGLRKEE+S+L G +E  EFYSRL+ +KE+ RK+PNE++
Sbjct: 44  RNLLERSVIAAESMANLYEDQDGLRKEEVSSLSGPNEFAEFYSRLRSLKEYHRKYPNEVT 103

Query: 366 VPM 374
            PM
Sbjct: 104 EPM 106



 Score = 42.7 bits (96), Expect = 0.005
 Identities = 40/148 (27%), Positives = 56/148 (37%)
 Frame = +1

Query: 73  QQRSYHEERERTMDAMVKEILHKKTGHXGDYKC*SSFEEFTWIDILKPL*DLKNXMKIKM 252
           QQR  HEERER  DAM +EILHK +                   ++     + N  + + 
Sbjct: 7   QQRRLHEERERLEDAMSQEILHKISRKATQINADHRTRNLLERSVIAAE-SMANLYEDQD 65

Query: 253 DYEKKKYQHC*VHMSXRSSIRDLSKLKSXIENIQMKYLYPCPVEFEEVANXRGKSLLRIT 432
              K++                L  LK        +   P  +EF  +   R  ++    
Sbjct: 66  GLRKEEVSSLSGPNEFAEFYSRLRSLKEYHRKYPNEVTEPMQMEFLRMKENRENNIEDNL 125

Query: 433 QFAXEXTDEEGYGKYFGPTIARYEKYIN 516
               E TDEEGYGKY       Y+KY+N
Sbjct: 126 PLV-EFTDEEGYGKYL-DLHEVYDKYLN 151


>UniRef50_Q16EE4 Cluster: Splicing factor 3a; n=2; Culicidae|Rep:
           Splicing factor 3a - Aedes aegypti (Yellowfever
           mosquito)
          Length = 484

 Score = 59.3 bits (137), Expect = 5e-08
 Identities = 27/64 (42%), Positives = 42/64 (65%)
 Frame = +3

Query: 186 RIYMDRYIEASIRLKELXEDKDGLRKEEISALLGXHEXQEFYSRLKQIKEFXRKHPNEIS 365
           +IY++RY   S  L EL +DKDG RK+EI+  +  +E +EFYS+   + EF + H N ++
Sbjct: 44  KIYLERYQSCSKSLLELYQDKDGERKQEITN-MSVNEFKEFYSQFNSLVEFHQNHGNNVA 102

Query: 366 VPMS 377
           +P S
Sbjct: 103 IPAS 106


>UniRef50_Q22469 Cluster: Putative uncharacterized protein; n=5;
           Bilateria|Rep: Putative uncharacterized protein -
           Caenorhabditis elegans
          Length = 500

 Score = 58.4 bits (135), Expect = 9e-08
 Identities = 24/64 (37%), Positives = 43/64 (67%)
 Frame = +3

Query: 186 RIYMDRYIEASIRLKELXEDKDGLRKEEISALLGXHEXQEFYSRLKQIKEFXRKHPNEIS 365
           + ++DRY   S  L +  +D+DG +  E+ ++ G +E  EFYSRLK IK+  R++P+E++
Sbjct: 44  KTFVDRYYSVSAELAKFYKDEDGSKSMEMDSVSGPNEFAEFYSRLKVIKDAHRRNPDELA 103

Query: 366 VPMS 377
            P++
Sbjct: 104 EPLT 107


>UniRef50_Q9FG01 Cluster: Splicing factor 3a; n=6;
           Magnoliophyta|Rep: Splicing factor 3a - Arabidopsis
           thaliana (Mouse-ear cress)
          Length = 504

 Score = 44.4 bits (100), Expect = 0.002
 Identities = 22/50 (44%), Positives = 32/50 (64%), Gaps = 5/50 (10%)
 Frame = +3

Query: 222 RLKELXEDKDGLRKEEISAL-----LGXHEXQEFYSRLKQIKEFXRKHPN 356
           +L E  EDKDG   +EI+AL      G +   EFY RLK+I+E+ ++HP+
Sbjct: 57  KLVETYEDKDGAWDDEIAALGGQTATGTNVFSEFYDRLKEIREYHKRHPS 106


>UniRef50_Q55CK7 Cluster: C2H2 type Zn finger-containing protein;
           n=1; Dictyostelium discoideum AX4|Rep: C2H2 type Zn
           finger-containing protein - Dictyostelium discoideum AX4
          Length = 544

 Score = 41.5 bits (93), Expect = 0.011
 Identities = 20/57 (35%), Positives = 33/57 (57%), Gaps = 2/57 (3%)
 Frame = +3

Query: 192 YMDRYIEASIRLKELXEDKDGLRKEEISAL--LGXHEXQEFYSRLKQIKEFXRKHPN 356
           Y++  IE S  L  +  D D  RK+E++++   G      FY +L++IK++ RK PN
Sbjct: 47  YLNSSIECSKSLINIYTDSDHSRKDELTSISGFGTDLYSSFYEKLREIKDYHRKFPN 103


>UniRef50_A4QU30 Cluster: Putative uncharacterized protein; n=1;
           Magnaporthe grisea|Rep: Putative uncharacterized protein
           - Magnaporthe grisea (Rice blast fungus) (Pyricularia
           grisea)
          Length = 521

 Score = 40.7 bits (91), Expect = 0.019
 Identities = 19/55 (34%), Positives = 32/55 (58%)
 Frame = +3

Query: 195 MDRYIEASIRLKELXEDKDGLRKEEISALLGXHEXQEFYSRLKQIKEFXRKHPNE 359
           +++  + S  L  + ED +GLR +EI A+       EFY ++++IKE   K+P E
Sbjct: 45  LEQIQKQSSELISMYEDVNGLRSKEIQAIGSGDPFDEFYKQVEEIKEHHAKYPTE 99


>UniRef50_O59706 Cluster: U2 snRNP-associated protein sap61; n=1;
           Schizosaccharomyces pombe|Rep: U2 snRNP-associated
           protein sap61 - Schizosaccharomyces pombe (Fission
           yeast)
          Length = 492

 Score = 38.7 bits (86), Expect = 0.078
 Identities = 16/55 (29%), Positives = 33/55 (60%)
 Frame = +3

Query: 192 YMDRYIEASIRLKELXEDKDGLRKEEISALLGXHEXQEFYSRLKQIKEFXRKHPN 356
           +++++ E S +L    E  D L+ +E++ +    +  EFY  L +I+EF +K+P+
Sbjct: 47  FLNQFRETSKKLLVSHESSDRLKDQEVARINADDDLTEFYKSLGEIQEFHKKYPD 101


>UniRef50_Q54UG9 Cluster: C2H2 type Zn finger-containing protein;
           n=1; Dictyostelium discoideum AX4|Rep: C2H2 type Zn
           finger-containing protein - Dictyostelium discoideum AX4
          Length = 576

 Score = 33.1 bits (72), Expect = 3.9
 Identities = 24/80 (30%), Positives = 32/80 (40%)
 Frame = +1

Query: 136 HKKTGHXGDYKC*SSFEEFTWIDILKPL*DLKNXMKIKMDYEKKKYQHC*VHMSXRSSIR 315
           H+KT H   Y C      F      K    L    K+ +D EK+K  HC  H        
Sbjct: 293 HRKTSHTITYNCDQCSSVF------KSSSKLSEHKKVHIDIEKRKAFHC-THEGCDKVYL 345

Query: 316 DLSKLKSXIENIQMKYLYPC 375
             S L S I++I +K  + C
Sbjct: 346 TKSGLDSHIKSIHLKQSFTC 365


>UniRef50_UPI00006CFDC8 Cluster: splicesome-associated protein,
           putative; n=1; Tetrahymena thermophila SB210|Rep:
           splicesome-associated protein, putative - Tetrahymena
           thermophila SB210
          Length = 521

 Score = 32.7 bits (71), Expect = 5.1
 Identities = 19/52 (36%), Positives = 27/52 (51%), Gaps = 10/52 (19%)
 Frame = +3

Query: 234 LXEDKDGLRKEEISALLGXHEXQ----------EFYSRLKQIKEFXRKHPNE 359
           L +D DG++KEE++ L G                F  RLK IKE+ +KH N+
Sbjct: 61  LLQDFDGVKKEEMAILEGKKSVSAGTRQPDIWYNFQQRLKDIKEYYKKHQNQ 112


>UniRef50_UPI00006CB7B0 Cluster: EF hand family protein; n=1;
           Tetrahymena thermophila SB210|Rep: EF hand family
           protein - Tetrahymena thermophila SB210
          Length = 349

 Score = 31.9 bits (69), Expect = 8.9
 Identities = 18/47 (38%), Positives = 28/47 (59%)
 Frame = +2

Query: 47  LMI*FFIISNNEATMRKGKEPWTQWSKKFFTKKLGIXETINADHRLK 187
           LM    II   E T+++ KE   QW+++  +KKL I E IN ++ L+
Sbjct: 17  LMFTLTIIYFGETTVKRVKEDIQQWNEEELSKKL-ITEKINLEYNLR 62


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 422,695,869
Number of Sequences: 1657284
Number of extensions: 6619957
Number of successful extensions: 16301
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 15906
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 16290
length of database: 575,637,011
effective HSP length: 95
effective length of database: 418,195,031
effective search space used: 31782822356
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)