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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30352
         (516 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g22520.1 68416.m02846 expressed protein                             30   1.1  
At3g16200.1 68416.m02045 expressed protein                             30   1.1  
At1g61370.1 68414.m06916 S-locus lectin protein kinase family pr...    30   1.1  
At2g20190.1 68415.m02361 CLIP-associating protein (CLASP) -relat...    29   1.9  
At3g60240.1 68416.m06732 MIF4G domain-containing protein / MA3 d...    28   3.2  
At1g61380.1 68414.m06917 S-locus protein kinase, putative simila...    28   4.3  
At1g20790.1 68414.m02603 F-box family protein contains Pfam:PF00...    28   4.3  
At5g57850.1 68418.m07234 aminotransferase class IV family protei...    27   5.7  
At5g55800.1 68418.m06954 DC1 domain-containing protein contains ...    27   5.7  
At3g63260.2 68416.m07109 protein kinase, putative (MRK1) identic...    27   5.7  
At3g63260.1 68416.m07108 protein kinase, putative (MRK1) identic...    27   5.7  
At4g20170.1 68417.m02950 expressed protein                             27   7.5  
At4g10950.1 68417.m01780 GDSL-motif lipase/hydrolase family prot...    27   9.9  
At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate 5-ki...    27   9.9  

>At3g22520.1 68416.m02846 expressed protein
          Length = 600

 Score = 29.9 bits (64), Expect = 1.1
 Identities = 17/50 (34%), Positives = 28/50 (56%)
 Frame = -1

Query: 477 PVSSRTIPQSTPHGTYGTKYRGNRSPSADPEAPNDP*EWDYRQSERPSSV 328
           PV ++  P+STP G+   K +G  +P+  P++   P     RQS R +S+
Sbjct: 251 PVEAK--PKSTPRGSSNKKKKGATTPATGPQSSTKP--KPSRQSGRRTSI 296


>At3g16200.1 68416.m02045 expressed protein
          Length = 452

 Score = 29.9 bits (64), Expect = 1.1
 Identities = 16/50 (32%), Positives = 21/50 (42%)
 Frame = -3

Query: 508 WRAVRW*KRDAGIISNNSSEHSPWNIRYKIQREPKSLRRPRGSKRSVRMG 359
           W   RW K D  I S     HS W +R  +   P     PR  ++ +R G
Sbjct: 173 WFMARWLKPDMMIESGAFKGHSTWVLRQAMPDTPMISLTPRHPEKYLRKG 222


>At1g61370.1 68414.m06916 S-locus lectin protein kinase family
           protein contains Pfam domains, PF00954: S-locus
           glycoprotein family, PF00069: Protein kinase domain, and
           PF01453: Lectin (probable mannose binding)
          Length = 814

 Score = 29.9 bits (64), Expect = 1.1
 Identities = 16/41 (39%), Positives = 20/41 (48%), Gaps = 3/41 (7%)
 Frame = +1

Query: 16  CVRALSKPSTSAASSFVCP---EWWKNVWPWSMMRRTNVSC 129
           C+R+ + P       FV     EW K  W    MRRTN+SC
Sbjct: 298 CIRS-NPPKCECLKGFVPKSDEEWNKRNWTGGCMRRTNLSC 337


>At2g20190.1 68415.m02361 CLIP-associating protein (CLASP) -related
           similar to CLIP-associating protein CLASP2 (GI:13508651)
           [Rattus norvegicus]
          Length = 1439

 Score = 29.1 bits (62), Expect = 1.9
 Identities = 14/38 (36%), Positives = 23/38 (60%)
 Frame = -3

Query: 388 RGSKRSVRMGLSTIRTALLCMESNGRSWYLGVLIASEN 275
           +GS R+  +GLS I T +   + +GRS Y G L++  +
Sbjct: 652 KGSNRNGGLGLSDIITQIQASKDSGRSSYRGNLLSESH 689


>At3g60240.1 68416.m06732 MIF4G domain-containing protein / MA3
           domain-containing protein similar to eukaryotic protein
           synthesis initiation factor [Homo sapiens] GI:3941724;
           contains Pfam profiles PF02854: MIF4G domain, PF02847:
           MA3 domain
          Length = 1544

 Score = 28.3 bits (60), Expect = 3.2
 Identities = 13/29 (44%), Positives = 19/29 (65%)
 Frame = +3

Query: 66  MSGMVEKCLAVVDDAEDECIVHTEVAMTD 152
           +SG++EK    VD A D C V  ++A+TD
Sbjct: 560 LSGVLEKAQNEVDGATDVCPVSEKLAVTD 588


>At1g61380.1 68414.m06917 S-locus protein kinase, putative similar
           to KI domain interacting kinase 1 [Zea mays]
           gi|2735017|gb|AAB93834; contains S-locus glycoprotein
           family domain, Pfam:PF00954
          Length = 805

 Score = 27.9 bits (59), Expect = 4.3
 Identities = 16/45 (35%), Positives = 20/45 (44%), Gaps = 3/45 (6%)
 Frame = +1

Query: 16  CVRALSKPSTSAASSFVCP---EWWKNVWPWSMMRRTNVSCTRRS 141
           CVR+   P       FV     EW K  W    +RRT +SC  +S
Sbjct: 293 CVRS-DPPKCECLKGFVPKSDEEWGKGNWTSGCVRRTKLSCQAKS 336


>At1g20790.1 68414.m02603 F-box family protein contains Pfam:PF00646
           F-box domain
          Length = 435

 Score = 27.9 bits (59), Expect = 4.3
 Identities = 19/82 (23%), Positives = 36/82 (43%)
 Frame = -3

Query: 499 VRW*KRDAGIISNNSSEHSPWNIRYKIQREPKSLRRPRGSKRSVRMGLSTIRTALLCMES 320
           + W ++D  I++ N        I+ +  R+P  L    G  R   +  +    ++  +E+
Sbjct: 199 LHWLRKDGSIVAFNPETEKARLIQSQFNRKPGKLLLCTGDNRLTLISATDAVISVYALET 258

Query: 319 NGRSWYLGVLIASENRMLQLPL 254
           +G+ W L   I +E     LPL
Sbjct: 259 DGQ-WILVRWIKNEVVHQSLPL 279


>At5g57850.1 68418.m07234 aminotransferase class IV family protein
           contains Pfam profile: PF01063 aminotransferase class IV
          Length = 373

 Score = 27.5 bits (58), Expect = 5.7
 Identities = 19/54 (35%), Positives = 30/54 (55%)
 Frame = -3

Query: 439 WNIRYKIQREPKSLRRPRGSKRSVRMGLSTIRTALLCMESNGRSWYLGVLIASE 278
           WN+R  + + P  LR   G +   R  L+  RT L+C +S+ +SW + VL + E
Sbjct: 13  WNLR-SLSQVPCPLRH--GFRFPRR--LTRRRTILMCSDSSSQSWNVPVLSSYE 61


>At5g55800.1 68418.m06954 DC1 domain-containing protein contains
           Pfam profile PF03107: DC1 domain
          Length = 578

 Score = 27.5 bits (58), Expect = 5.7
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = +3

Query: 420 ILYRMFHGECSEELFEMIPASRFYH 494
           I  +MFH EC E  FE+I  S  +H
Sbjct: 108 ICEKMFHKECVESPFEIIHPSHPFH 132


>At3g63260.2 68416.m07109 protein kinase, putative (MRK1) identical
           to ATMRK1 [Arabidopsis thaliana] gi|2351097|dbj|BAA22079
          Length = 344

 Score = 27.5 bits (58), Expect = 5.7
 Identities = 19/69 (27%), Positives = 29/69 (42%), Gaps = 1/69 (1%)
 Frame = -1

Query: 483 EMPVSSRTIPQSTPHGTYGTKYRGNRSPSADPEAPNDP*EWDYRQSERPSSVWSQME-EA 307
           E+ +S   +     HGTYGT YRG  +         D  E  Y      +++ +  E E 
Sbjct: 77  EIDLSKLDMKHVLAHGTYGTVYRGVYAGQEVAVKVLDWGEDGYATPAETTALRASFEQEV 136

Query: 306 GIWEY**HP 280
            +W+   HP
Sbjct: 137 AVWQKLDHP 145


>At3g63260.1 68416.m07108 protein kinase, putative (MRK1) identical
           to ATMRK1 [Arabidopsis thaliana] gi|2351097|dbj|BAA22079
          Length = 391

 Score = 27.5 bits (58), Expect = 5.7
 Identities = 19/69 (27%), Positives = 29/69 (42%), Gaps = 1/69 (1%)
 Frame = -1

Query: 483 EMPVSSRTIPQSTPHGTYGTKYRGNRSPSADPEAPNDP*EWDYRQSERPSSVWSQME-EA 307
           E+ +S   +     HGTYGT YRG  +         D  E  Y      +++ +  E E 
Sbjct: 77  EIDLSKLDMKHVLAHGTYGTVYRGVYAGQEVAVKVLDWGEDGYATPAETTALRASFEQEV 136

Query: 306 GIWEY**HP 280
            +W+   HP
Sbjct: 137 AVWQKLDHP 145


>At4g20170.1 68417.m02950 expressed protein
          Length = 504

 Score = 27.1 bits (57), Expect = 7.5
 Identities = 11/41 (26%), Positives = 21/41 (51%)
 Frame = -2

Query: 476 RYHLEQFLRALPMEHTVQNTEGTEVPPQTQRLQTIRENGII 354
           R+ + +F  A+P+  T    E +  P   + L  + +NG+I
Sbjct: 60  RFCISRFSSAVPLNTTTTVEESSSSPSPEKNLDRVLDNGVI 100


>At4g10950.1 68417.m01780 GDSL-motif lipase/hydrolase family protein
           similar to family II lipase EXL1 GI:15054382 from
           [Arabidopsis thaliana]; contains Pfam profile PF00657:
           GDSL-like Lipase/Acylhydrolase
          Length = 400

 Score = 26.6 bits (56), Expect = 9.9
 Identities = 12/24 (50%), Positives = 15/24 (62%)
 Frame = +3

Query: 426 YRMFHGECSEELFEMIPASRFYHR 497
           YR  +GEC+EE+  MI  S F  R
Sbjct: 269 YRSQNGECAEEVNSMIMESNFVMR 292


>At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate
           5-kinase, putative / PIP kinase, putative /
           PtdIns(4)P-5-kinase, putative / diphosphoinositide
           kinase, putative similar to
           phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
           [Arabidopsis thaliana] GI:3702691; contains Pfam
           profiles PF01504: Phosphatidylinositol-4-phosphate
           5-Kinase, PF02493: MORN repeat
          Length = 705

 Score = 26.6 bits (56), Expect = 9.9
 Identities = 15/45 (33%), Positives = 20/45 (44%)
 Frame = -2

Query: 173 LAYGRLFIGHSDLRVHDTFVLRIIDHGQTFFHHSGHTKEEAALVD 39
           +  G LF   S+ R+H  F L+   HG+T     G   E   L D
Sbjct: 491 IVMGNLFC--SEYRIHKRFDLKGSSHGRTIDKDEGEIDETTTLKD 533


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,674,203
Number of Sequences: 28952
Number of extensions: 271622
Number of successful extensions: 817
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 800
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 817
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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