BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30352 (516 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g22520.1 68416.m02846 expressed protein 30 1.1 At3g16200.1 68416.m02045 expressed protein 30 1.1 At1g61370.1 68414.m06916 S-locus lectin protein kinase family pr... 30 1.1 At2g20190.1 68415.m02361 CLIP-associating protein (CLASP) -relat... 29 1.9 At3g60240.1 68416.m06732 MIF4G domain-containing protein / MA3 d... 28 3.2 At1g61380.1 68414.m06917 S-locus protein kinase, putative simila... 28 4.3 At1g20790.1 68414.m02603 F-box family protein contains Pfam:PF00... 28 4.3 At5g57850.1 68418.m07234 aminotransferase class IV family protei... 27 5.7 At5g55800.1 68418.m06954 DC1 domain-containing protein contains ... 27 5.7 At3g63260.2 68416.m07109 protein kinase, putative (MRK1) identic... 27 5.7 At3g63260.1 68416.m07108 protein kinase, putative (MRK1) identic... 27 5.7 At4g20170.1 68417.m02950 expressed protein 27 7.5 At4g10950.1 68417.m01780 GDSL-motif lipase/hydrolase family prot... 27 9.9 At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate 5-ki... 27 9.9 >At3g22520.1 68416.m02846 expressed protein Length = 600 Score = 29.9 bits (64), Expect = 1.1 Identities = 17/50 (34%), Positives = 28/50 (56%) Frame = -1 Query: 477 PVSSRTIPQSTPHGTYGTKYRGNRSPSADPEAPNDP*EWDYRQSERPSSV 328 PV ++ P+STP G+ K +G +P+ P++ P RQS R +S+ Sbjct: 251 PVEAK--PKSTPRGSSNKKKKGATTPATGPQSSTKP--KPSRQSGRRTSI 296 >At3g16200.1 68416.m02045 expressed protein Length = 452 Score = 29.9 bits (64), Expect = 1.1 Identities = 16/50 (32%), Positives = 21/50 (42%) Frame = -3 Query: 508 WRAVRW*KRDAGIISNNSSEHSPWNIRYKIQREPKSLRRPRGSKRSVRMG 359 W RW K D I S HS W +R + P PR ++ +R G Sbjct: 173 WFMARWLKPDMMIESGAFKGHSTWVLRQAMPDTPMISLTPRHPEKYLRKG 222 >At1g61370.1 68414.m06916 S-locus lectin protein kinase family protein contains Pfam domains, PF00954: S-locus glycoprotein family, PF00069: Protein kinase domain, and PF01453: Lectin (probable mannose binding) Length = 814 Score = 29.9 bits (64), Expect = 1.1 Identities = 16/41 (39%), Positives = 20/41 (48%), Gaps = 3/41 (7%) Frame = +1 Query: 16 CVRALSKPSTSAASSFVCP---EWWKNVWPWSMMRRTNVSC 129 C+R+ + P FV EW K W MRRTN+SC Sbjct: 298 CIRS-NPPKCECLKGFVPKSDEEWNKRNWTGGCMRRTNLSC 337 >At2g20190.1 68415.m02361 CLIP-associating protein (CLASP) -related similar to CLIP-associating protein CLASP2 (GI:13508651) [Rattus norvegicus] Length = 1439 Score = 29.1 bits (62), Expect = 1.9 Identities = 14/38 (36%), Positives = 23/38 (60%) Frame = -3 Query: 388 RGSKRSVRMGLSTIRTALLCMESNGRSWYLGVLIASEN 275 +GS R+ +GLS I T + + +GRS Y G L++ + Sbjct: 652 KGSNRNGGLGLSDIITQIQASKDSGRSSYRGNLLSESH 689 >At3g60240.1 68416.m06732 MIF4G domain-containing protein / MA3 domain-containing protein similar to eukaryotic protein synthesis initiation factor [Homo sapiens] GI:3941724; contains Pfam profiles PF02854: MIF4G domain, PF02847: MA3 domain Length = 1544 Score = 28.3 bits (60), Expect = 3.2 Identities = 13/29 (44%), Positives = 19/29 (65%) Frame = +3 Query: 66 MSGMVEKCLAVVDDAEDECIVHTEVAMTD 152 +SG++EK VD A D C V ++A+TD Sbjct: 560 LSGVLEKAQNEVDGATDVCPVSEKLAVTD 588 >At1g61380.1 68414.m06917 S-locus protein kinase, putative similar to KI domain interacting kinase 1 [Zea mays] gi|2735017|gb|AAB93834; contains S-locus glycoprotein family domain, Pfam:PF00954 Length = 805 Score = 27.9 bits (59), Expect = 4.3 Identities = 16/45 (35%), Positives = 20/45 (44%), Gaps = 3/45 (6%) Frame = +1 Query: 16 CVRALSKPSTSAASSFVCP---EWWKNVWPWSMMRRTNVSCTRRS 141 CVR+ P FV EW K W +RRT +SC +S Sbjct: 293 CVRS-DPPKCECLKGFVPKSDEEWGKGNWTSGCVRRTKLSCQAKS 336 >At1g20790.1 68414.m02603 F-box family protein contains Pfam:PF00646 F-box domain Length = 435 Score = 27.9 bits (59), Expect = 4.3 Identities = 19/82 (23%), Positives = 36/82 (43%) Frame = -3 Query: 499 VRW*KRDAGIISNNSSEHSPWNIRYKIQREPKSLRRPRGSKRSVRMGLSTIRTALLCMES 320 + W ++D I++ N I+ + R+P L G R + + ++ +E+ Sbjct: 199 LHWLRKDGSIVAFNPETEKARLIQSQFNRKPGKLLLCTGDNRLTLISATDAVISVYALET 258 Query: 319 NGRSWYLGVLIASENRMLQLPL 254 +G+ W L I +E LPL Sbjct: 259 DGQ-WILVRWIKNEVVHQSLPL 279 >At5g57850.1 68418.m07234 aminotransferase class IV family protein contains Pfam profile: PF01063 aminotransferase class IV Length = 373 Score = 27.5 bits (58), Expect = 5.7 Identities = 19/54 (35%), Positives = 30/54 (55%) Frame = -3 Query: 439 WNIRYKIQREPKSLRRPRGSKRSVRMGLSTIRTALLCMESNGRSWYLGVLIASE 278 WN+R + + P LR G + R L+ RT L+C +S+ +SW + VL + E Sbjct: 13 WNLR-SLSQVPCPLRH--GFRFPRR--LTRRRTILMCSDSSSQSWNVPVLSSYE 61 >At5g55800.1 68418.m06954 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 578 Score = 27.5 bits (58), Expect = 5.7 Identities = 12/25 (48%), Positives = 15/25 (60%) Frame = +3 Query: 420 ILYRMFHGECSEELFEMIPASRFYH 494 I +MFH EC E FE+I S +H Sbjct: 108 ICEKMFHKECVESPFEIIHPSHPFH 132 >At3g63260.2 68416.m07109 protein kinase, putative (MRK1) identical to ATMRK1 [Arabidopsis thaliana] gi|2351097|dbj|BAA22079 Length = 344 Score = 27.5 bits (58), Expect = 5.7 Identities = 19/69 (27%), Positives = 29/69 (42%), Gaps = 1/69 (1%) Frame = -1 Query: 483 EMPVSSRTIPQSTPHGTYGTKYRGNRSPSADPEAPNDP*EWDYRQSERPSSVWSQME-EA 307 E+ +S + HGTYGT YRG + D E Y +++ + E E Sbjct: 77 EIDLSKLDMKHVLAHGTYGTVYRGVYAGQEVAVKVLDWGEDGYATPAETTALRASFEQEV 136 Query: 306 GIWEY**HP 280 +W+ HP Sbjct: 137 AVWQKLDHP 145 >At3g63260.1 68416.m07108 protein kinase, putative (MRK1) identical to ATMRK1 [Arabidopsis thaliana] gi|2351097|dbj|BAA22079 Length = 391 Score = 27.5 bits (58), Expect = 5.7 Identities = 19/69 (27%), Positives = 29/69 (42%), Gaps = 1/69 (1%) Frame = -1 Query: 483 EMPVSSRTIPQSTPHGTYGTKYRGNRSPSADPEAPNDP*EWDYRQSERPSSVWSQME-EA 307 E+ +S + HGTYGT YRG + D E Y +++ + E E Sbjct: 77 EIDLSKLDMKHVLAHGTYGTVYRGVYAGQEVAVKVLDWGEDGYATPAETTALRASFEQEV 136 Query: 306 GIWEY**HP 280 +W+ HP Sbjct: 137 AVWQKLDHP 145 >At4g20170.1 68417.m02950 expressed protein Length = 504 Score = 27.1 bits (57), Expect = 7.5 Identities = 11/41 (26%), Positives = 21/41 (51%) Frame = -2 Query: 476 RYHLEQFLRALPMEHTVQNTEGTEVPPQTQRLQTIRENGII 354 R+ + +F A+P+ T E + P + L + +NG+I Sbjct: 60 RFCISRFSSAVPLNTTTTVEESSSSPSPEKNLDRVLDNGVI 100 >At4g10950.1 68417.m01780 GDSL-motif lipase/hydrolase family protein similar to family II lipase EXL1 GI:15054382 from [Arabidopsis thaliana]; contains Pfam profile PF00657: GDSL-like Lipase/Acylhydrolase Length = 400 Score = 26.6 bits (56), Expect = 9.9 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +3 Query: 426 YRMFHGECSEELFEMIPASRFYHR 497 YR +GEC+EE+ MI S F R Sbjct: 269 YRSQNGECAEEVNSMIMESNFVMR 292 >At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate 5-kinase, putative / PIP kinase, putative / PtdIns(4)P-5-kinase, putative / diphosphoinositide kinase, putative similar to phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1 [Arabidopsis thaliana] GI:3702691; contains Pfam profiles PF01504: Phosphatidylinositol-4-phosphate 5-Kinase, PF02493: MORN repeat Length = 705 Score = 26.6 bits (56), Expect = 9.9 Identities = 15/45 (33%), Positives = 20/45 (44%) Frame = -2 Query: 173 LAYGRLFIGHSDLRVHDTFVLRIIDHGQTFFHHSGHTKEEAALVD 39 + G LF S+ R+H F L+ HG+T G E L D Sbjct: 491 IVMGNLFC--SEYRIHKRFDLKGSSHGRTIDKDEGEIDETTTLKD 533 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,674,203 Number of Sequences: 28952 Number of extensions: 271622 Number of successful extensions: 817 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 800 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 817 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 937669760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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