BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30339 (438 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g18540.1 68415.m02160 cupin family protein contains Pfam prof... 31 0.34 At1g06720.1 68414.m00714 expressed protein contains Pfam domain,... 31 0.34 At3g07650.2 68416.m00917 zinc finger (B-box type) family protein... 30 0.59 At3g07650.1 68416.m00916 zinc finger (B-box type) family protein... 30 0.59 At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N domain-... 29 1.0 At4g36700.1 68417.m05208 cupin family protein low similarity to ... 29 1.8 At5g03660.1 68418.m00325 expressed protein low similarity to out... 27 5.5 At1g11240.1 68414.m01287 expressed protein 27 5.5 At5g61320.1 68418.m07695 cytochrome P450, putative Similar to C... 27 7.3 At1g26790.1 68414.m03261 Dof-type zinc finger domain-containing ... 27 7.3 At5g04890.1 68418.m00513 small heat shock-like protein (RTM2) si... 26 9.7 At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein /... 26 9.7 >At2g18540.1 68415.m02160 cupin family protein contains Pfam profile PF00190: Cupin Length = 707 Score = 31.1 bits (67), Expect = 0.34 Identities = 15/29 (51%), Positives = 19/29 (65%) Frame = +3 Query: 84 EKKRLEEANKAKQAEIDRKRAEVRKRMEE 170 E+KR EE + ++ E RKR E RKR EE Sbjct: 546 ERKRREEQERKRREEEARKREEERKREEE 574 Score = 28.7 bits (61), Expect = 1.8 Identities = 15/30 (50%), Positives = 20/30 (66%), Gaps = 2/30 (6%) Frame = +3 Query: 87 KKRLEEANKAKQAEIDRKRAE--VRKRMEE 170 KKR EE + ++ E++RKR E RKR EE Sbjct: 531 KKREEERQRKEREEVERKRREEQERKRREE 560 Score = 27.5 bits (58), Expect = 4.2 Identities = 12/31 (38%), Positives = 19/31 (61%) Frame = +3 Query: 81 DEKKRLEEANKAKQAEIDRKRAEVRKRMEEA 173 +E ++ EE K ++ E R+ E +KR EEA Sbjct: 483 EEARKREEERKREEEEAKRREEERKKREEEA 513 Score = 27.5 bits (58), Expect = 4.2 Identities = 14/31 (45%), Positives = 21/31 (67%), Gaps = 1/31 (3%) Frame = +3 Query: 81 DEKKRLEEANKAKQAEIDR-KRAEVRKRMEE 170 + KKR EEA +A++ E +R K E+ K+ EE Sbjct: 505 ERKKREEEAEQARKREEEREKEEEMAKKREE 535 Score = 26.2 bits (55), Expect = 9.7 Identities = 15/32 (46%), Positives = 20/32 (62%), Gaps = 2/32 (6%) Frame = +3 Query: 81 DEKKRLEEANKAKQAEID--RKRAEVRKRMEE 170 + K+R EE + K+ E + RKR E RKR EE Sbjct: 466 EAKRREEEETERKKREEEEARKREEERKREEE 497 >At1g06720.1 68414.m00714 expressed protein contains Pfam domain, PF04950: Protein of unknown function (DUF663) Length = 1147 Score = 31.1 bits (67), Expect = 0.34 Identities = 16/30 (53%), Positives = 20/30 (66%), Gaps = 1/30 (3%) Frame = +3 Query: 75 AVDEKKRLE-EANKAKQAEIDRKRAEVRKR 161 A D+KKR E EA KAK EI++KR +R Sbjct: 1100 ATDQKKRKEYEAEKAKNEEINKKRRREERR 1129 >At3g07650.2 68416.m00917 zinc finger (B-box type) family protein similar to zinc finger protein GB:BAA33206 [Oryza sativa] Length = 372 Score = 30.3 bits (65), Expect = 0.59 Identities = 14/46 (30%), Positives = 26/46 (56%) Frame = +3 Query: 30 SNSCSRTP*TNTTTMAVDEKKRLEEANKAKQAEIDRKRAEVRKRME 167 +N+CS + N M EKK+ + +K + + RA+VR+R++ Sbjct: 306 NNACSHSVTRNNAVMRYKEKKKARKFDKRVRYASRKARADVRRRVK 351 >At3g07650.1 68416.m00916 zinc finger (B-box type) family protein similar to zinc finger protein GB:BAA33206 [Oryza sativa] Length = 372 Score = 30.3 bits (65), Expect = 0.59 Identities = 14/46 (30%), Positives = 26/46 (56%) Frame = +3 Query: 30 SNSCSRTP*TNTTTMAVDEKKRLEEANKAKQAEIDRKRAEVRKRME 167 +N+CS + N M EKK+ + +K + + RA+VR+R++ Sbjct: 306 NNACSHSVTRNNAVMRYKEKKKARKFDKRVRYASRKARADVRRRVK 351 >At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N domain-containing protein weak similarity to SP|P45974 Ubiquitin carboxyl-terminal hydrolase 5 (EC 3.1.2.15) {Homo sapiens}; contains Pfam profile PF00627: UBA/TS-N domain Length = 413 Score = 29.5 bits (63), Expect = 1.0 Identities = 13/31 (41%), Positives = 22/31 (70%) Frame = +3 Query: 78 VDEKKRLEEANKAKQAEIDRKRAEVRKRMEE 170 V+E+KRL KA++ E R R ++R+++EE Sbjct: 227 VNERKRLMFLRKAEKEEEKRAREKIRQKLEE 257 >At4g36700.1 68417.m05208 cupin family protein low similarity to preproMP27-MP32 from Cucurbita cv. Kurokawa Amakuri [GI:691752]; contains Pfam profile PF00190: Cupin Length = 522 Score = 28.7 bits (61), Expect = 1.8 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = +3 Query: 81 DEKKRLEEANKAKQAEIDRKRAEVRKRMEE 170 DE+KR + K ++ E R+ E RKR EE Sbjct: 457 DERKRRHDERKKEEEEAKREEEERRKREEE 486 >At5g03660.1 68418.m00325 expressed protein low similarity to outer surface protein F [Borrelia burgdorferi] GI:466482; contains Pfam profile PF04949: Family of unknown function (DUF662) Length = 173 Score = 27.1 bits (57), Expect = 5.5 Identities = 10/21 (47%), Positives = 18/21 (85%) Frame = +3 Query: 105 ANKAKQAEIDRKRAEVRKRME 167 A KAK+ EI++K+ E+R+R++ Sbjct: 46 AFKAKEEEIEKKKMEIRERVQ 66 >At1g11240.1 68414.m01287 expressed protein Length = 200 Score = 27.1 bits (57), Expect = 5.5 Identities = 14/31 (45%), Positives = 20/31 (64%), Gaps = 2/31 (6%) Frame = +3 Query: 84 EKKRLEEANKAKQAEIDRKRAEVRK--RMEE 170 +KKR +EA K ++ RKR E RK ++EE Sbjct: 52 KKKRRKEAQKQQEEAFRRKRIEARKNRKLEE 82 >At5g61320.1 68418.m07695 cytochrome P450, putative Similar to Cytochrome P450 89A2 (SP:Q42602)[Arabidopsis thaliana]; contains Pfam profile: PF00067 cytochrome P450 Length = 497 Score = 26.6 bits (56), Expect = 7.3 Identities = 9/26 (34%), Positives = 19/26 (73%) Frame = +1 Query: 73 WRLMKKSVSRRXIRPNRPKSTASALR 150 WRL++++++ + P+R KS ++A R Sbjct: 127 WRLLRRNLTSEILHPSRVKSYSNARR 152 >At1g26790.1 68414.m03261 Dof-type zinc finger domain-containing protein similar to H-protein promoter binding factor-2b GI:3386548 from [Arabidopsis thaliana] Length = 396 Score = 26.6 bits (56), Expect = 7.3 Identities = 9/17 (52%), Positives = 11/17 (64%), Gaps = 1/17 (5%) Frame = -1 Query: 123 PVWPYX-PPRDAFFHQP 76 P WPY PP +F+H P Sbjct: 262 PPWPYQYPPNPSFYHMP 278 >At5g04890.1 68418.m00513 small heat shock-like protein (RTM2) similar to 17.9 kDa heat-shock protein [Helianthus annuus] GI:11990130; contains Pfam profile PF00011: Hsp20/alpha crystallin family; supporting cDNA gi|7407072|gb|AF208051.1|AF208051; identical to cDNA small heat shock-like protein (RTM2) GI:7407072, small heat shock-like protein [Arabidopsis thaliana] GI:7407073 Length = 366 Score = 26.2 bits (55), Expect = 9.7 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +3 Query: 84 EKKRLEEANKAKQAEIDRKRAEVRKRMEEA 173 ++K EEA + K+ ++ K A +RK EEA Sbjct: 141 KEKEEEEAKQMKKQLLEEKEALIRKLQEEA 170 >At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus; similar to cytosolic factor (Phosphatidylinositol/phosphatidylcholine transfer protein) (PI/PCTP) (SP:P24280) [Saccharomyces cerevisiae]; ESTs gb|T76582, gb|N06574 and gb|Z25700 come from this gene Length = 540 Score = 26.2 bits (55), Expect = 9.7 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = +3 Query: 87 KKRLEEANKAKQAEIDRKRAEVRKRMEEAS 176 KK+ K K+ E+ + AEV K+ EEA+ Sbjct: 92 KKKESSPMKEKKEEVVKPEAEVEKKKEEAA 121 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 5,992,128 Number of Sequences: 28952 Number of extensions: 75183 Number of successful extensions: 344 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 315 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 342 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 692941200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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