BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30329
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g24850.1 68416.m03117 hypothetical protein contains Pfam prof... 33 0.11
At5g22390.1 68418.m02612 expressed protein 30 1.1
At1g14830.1 68414.m01774 dynamin-like protein C (DL1C) nearly id... 28 3.2
At3g60450.1 68416.m06761 expressed protein 28 4.3
At4g38040.1 68417.m05373 exostosin family protein contains Pfam ... 27 5.7
At5g58980.1 68418.m07389 ceramidase family protein contains Pfam... 27 7.5
At5g13630.1 68418.m01580 magnesium-chelatase subunit chlH, chlor... 27 7.5
At4g19450.1 68417.m02861 nodulin-related weak similarity to nodu... 27 7.5
At1g78880.1 68414.m09195 balbiani ring 1-related / BR1-related c... 27 7.5
At1g78260.1 68414.m09120 RNA recognition motif (RRM)-containing ... 27 7.5
At1g18270.1 68414.m02280 ketose-bisphosphate aldolase class-II f... 27 7.5
At5g51530.1 68418.m06390 ubiquitin carboxyl-terminal hydrolase-r... 27 9.9
At5g39480.1 68418.m04781 F-box family protein contains Pfam:PF00... 27 9.9
At5g27540.1 68418.m03297 GTP-binding protein-related low similar... 27 9.9
At4g39450.1 68417.m05582 expressed protein 27 9.9
At4g26080.1 68417.m03755 protein phosphatase 2C ABI1 / PP2C ABI1... 27 9.9
At1g18780.1 68414.m02341 zinc finger (C3HC4-type RING finger) fa... 27 9.9
>At3g24850.1 68416.m03117 hypothetical protein contains Pfam profile
PF03754: Domain of unknown function (DUF313)
Length = 359
Score = 33.1 bits (72), Expect = 0.11
Identities = 25/95 (26%), Positives = 40/95 (42%), Gaps = 5/95 (5%)
Frame = +3
Query: 159 NTSFKSDTCKS--KLNELPSFATLDDLHLKPSRQKRNPASATQPCRMDTCFDFSKCGS-- 326
N F S T S LN+LP+ + ++D S+ K S+++PC FD+ S
Sbjct: 72 NGGFISFTSSSLIDLNQLPTDSEIEDPQTSDSQMKTLQNSSSEPCTSLVLFDYKTAESEK 131
Query: 327 -DPKIFVYPSDGTVSASYRKVLSVIRESRYVTRDP 428
+ K PS S+ + + T+DP
Sbjct: 132 MEAKTLQNPSSELCSSLVLSDYKMAESEKMETKDP 166
>At5g22390.1 68418.m02612 expressed protein
Length = 202
Score = 29.9 bits (64), Expect = 1.1
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = -3
Query: 346 YTKIFGSLPHLEKSKHVSIRQGCVAEAGLRFCLDGLRCRS 227
+T+IFG L H +S H S + + L+ C +GL S
Sbjct: 52 FTEIFGEL-HFRESSHSSYEKTSAENSSLQLCTEGLGSES 90
>At1g14830.1 68414.m01774 dynamin-like protein C (DL1C) nearly
identical to dynamin-like protein C [Arabidopsis
thaliana] GI:19569772
Length = 614
Score = 28.3 bits (60), Expect = 3.2
Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 4/64 (6%)
Frame = +3
Query: 231 LHLKPSRQKRNPASATQPCRMDTCFD--FSKCGSDPKIFVYPSDGTVSASYRK--VLSVI 398
LHL+P ++K NP +A P D D F K GS+ ++ T+ S K V +
Sbjct: 496 LHLEPEKEKPNPRNAPAP-NADPYSDNHFRKIGSNVSAYINMVCDTLRNSLPKAVVYCQV 554
Query: 399 RESR 410
RE++
Sbjct: 555 REAK 558
>At3g60450.1 68416.m06761 expressed protein
Length = 274
Score = 27.9 bits (59), Expect = 4.3
Identities = 22/58 (37%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = -3
Query: 325 LPHLEKSK-HVSIRQGCVAEAGLRFCLDGLRCRSSSVAKDGNSFNFDLQVSDLKLVFP 155
+P ++KSK VSI E GL L+ + R KDG FD VSD++ +FP
Sbjct: 103 VPSIDKSKLKVSI------ELGLCEMLNSVAIRRELAPKDGK---FDFTVSDIETMFP 151
>At4g38040.1 68417.m05373 exostosin family protein contains Pfam
profile: PF03016 Exostosin family
Length = 425
Score = 27.5 bits (58), Expect = 5.7
Identities = 18/43 (41%), Positives = 22/43 (51%), Gaps = 3/43 (6%)
Frame = +3
Query: 327 DPKIFVYPSDGTVSASYRK---VLSVIRESRYVTRDPNEACLF 446
DP F Y + V+ Y IRESR+ T DP+EA LF
Sbjct: 113 DPNTF-YQTPRKVTGKYASEGYFFQNIRESRFRTLDPDEADLF 154
>At5g58980.1 68418.m07389 ceramidase family protein contains Pfam
domain, PF04734: Neutral/alkaline nonlysosomal
ceramidase
Length = 733
Score = 27.1 bits (57), Expect = 7.5
Identities = 11/33 (33%), Positives = 22/33 (66%)
Frame = -3
Query: 466 SVSIAGTNKHASFGSLVTYLDSLITDNTFLYEA 368
+V+I+GT+ HA G + Y+ L+T F++++
Sbjct: 113 NVAISGTHTHAGPGGYLQYILYLVTSLGFVHQS 145
>At5g13630.1 68418.m01580 magnesium-chelatase subunit chlH,
chloroplast, putative / Mg-protoporphyrin IX chelatase,
putative (CHLH) nearly identical to magnesium chelatase
subunit GI:1154627 from [Arabidopsis thaliana]; contains
Pfam profile: PF02514 CobN/magnesium chelatase family
protein
Length = 1381
Score = 27.1 bits (57), Expect = 7.5
Identities = 14/51 (27%), Positives = 22/51 (43%)
Frame = +3
Query: 294 DTCFDFSKCGSDPKIFVYPSDGTVSASYRKVLSVIRESRYVTRDPNEACLF 446
D CF S G+ P ++ Y ++ A+ K S Y+T A L+
Sbjct: 683 DACFPDSLIGNIPNVYYYAANNPSEATIAKRRSYANTISYLTPPAENAGLY 733
>At4g19450.1 68417.m02861 nodulin-related weak similarity to
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 572
Score = 27.1 bits (57), Expect = 7.5
Identities = 10/28 (35%), Positives = 18/28 (64%)
Frame = +1
Query: 286 VVWIHVSTFLNVEVIQKFLCTQVMGLSV 369
V W+ ++ F+++ I FLC + GLS+
Sbjct: 88 VQWLVITHFISLPYIMVFLCCLLAGLSI 115
>At1g78880.1 68414.m09195 balbiani ring 1-related / BR1-related
contains weak similarity to BR1 [Chironomus tentans]
gi|7042|emb|CAA45607
Length = 468
Score = 27.1 bits (57), Expect = 7.5
Identities = 14/43 (32%), Positives = 20/43 (46%)
Frame = +3
Query: 240 KPSRQKRNPASATQPCRMDTCFDFSKCGSDPKIFVYPSDGTVS 368
+P + K P + + T D K G K+F P+DGT S
Sbjct: 19 RPEQPKEKPPTMSSVAMPYTGGDIKKSGELGKMFDIPTDGTKS 61
>At1g78260.1 68414.m09120 RNA recognition motif (RRM)-containing
protein similar to RNA recognition motif-containing
protein SEB-4 GI:8895698 from [Xenopus laevis]; contains
InterPro entry IPR000504: RNA-binding region RNP-1 (RNA
recognition motif) (RRM)
Length = 287
Score = 27.1 bits (57), Expect = 7.5
Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 5/60 (8%)
Frame = +3
Query: 324 SDPKIFVYPSDGTVSASYRKVLSVIRESRYVTRD----PNEACLFVPAIDTLDA-DPLSS 488
S P VYPS+ + + + VLS ES V + EA P I T + +P+SS
Sbjct: 228 SSPSFLVYPSNSSFAPPLQGVLSSSTESEAVPQQVPAAEGEATTTAPEITTSNTKEPISS 287
>At1g18270.1 68414.m02280 ketose-bisphosphate aldolase class-II family
protein low similarity to KbaY (tagatose-1,6-bisphosphate
aldolase) [Escherichia coli] GI:8895753; contains Pfam
profile PF01116: Fructose-bisphosphate aldolase class-II
Length = 1373
Score = 27.1 bits (57), Expect = 7.5
Identities = 19/54 (35%), Positives = 30/54 (55%)
Frame = -3
Query: 451 GTNKHASFGSLVTYLDSLITDNTFLYEALTVPSLGYTKIFGSLPHLEKSKHVSI 290
GT KH +L LDS++ D + L + T +L YTK S+ L +SK++ +
Sbjct: 1175 GTTKHELLEALELGLDSVMVDGSHL--SFT-ENLSYTK---SITELARSKNIMV 1222
>At5g51530.1 68418.m06390 ubiquitin carboxyl-terminal
hydrolase-related contains Pfam profiles PF00443:
Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
of unknown function (DUF629), PF04781: Protein of
unknown function (DUF627)
Length = 1149
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +1
Query: 52 VKQILVFISTVVKCKRNDDT 111
+ QIL F+ T+ KCKRND T
Sbjct: 404 LNQILDFLKTI-KCKRNDGT 422
>At5g39480.1 68418.m04781 F-box family protein contains Pfam:PF00646
F-box domain ; similar to SKP1 interacting partner 2
(SKIP2) TIGR_Ath1:At5g67250
Length = 568
Score = 26.6 bits (56), Expect = 9.9
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = +3
Query: 393 VIRESRYVTRDPNEACLFVPAIDTLDADP 479
++ RYV R PN +FV IDT DP
Sbjct: 437 ILEGKRYV-RHPNGTAMFVVKIDTPSLDP 464
>At5g27540.1 68418.m03297 GTP-binding protein-related low similarity
to Mig-2-like GTPase Mtl [Drosophila melanogaster]
GI:7271872; contains Pfam profile PF00036: EF hand
Length = 648
Score = 26.6 bits (56), Expect = 9.9
Identities = 19/60 (31%), Positives = 30/60 (50%), Gaps = 2/60 (3%)
Frame = +3
Query: 312 SKCGSDPKIFVYPSDGTVSASYRKVLSVIRE-SRYVTRDPNEA-CLFVPAIDTLDADPLS 485
S D +FVY D + +S+++ ++ E + Y E CL V A D LD+ P+S
Sbjct: 499 SLAACDIAVFVY--DSSDESSWKRATQLLVEVANYGEATGYEVPCLMVSAKDDLDSSPIS 556
>At4g39450.1 68417.m05582 expressed protein
Length = 1553
Score = 26.6 bits (56), Expect = 9.9
Identities = 22/90 (24%), Positives = 36/90 (40%), Gaps = 7/90 (7%)
Frame = -3
Query: 502 SDTCSDDKGSASSVSIAGTNKHASFGSLVT-------YLDSLITDNTFLYEALTVPSLGY 344
++ D+K SSV +++HAS +V +L L + FL +P
Sbjct: 505 TEAAEDEKAEDSSVIDDSSDEHASLSKMVAVLCEQRLFLPLLKAFDLFLPSCSLLPFFRA 564
Query: 343 TKIFGSLPHLEKSKHVSIRQGCVAEAGLRF 254
+ F + E S H+ G V E + F
Sbjct: 565 LQAFSQMRLSEASAHLGSFWGRVKEESMHF 594
>At4g26080.1 68417.m03755 protein phosphatase 2C ABI1 / PP2C ABI1 /
abscisic acid-insensitive 1 (ABI1) nearly identical to
SP|P49597 Protein phosphatase 2C ABI1 (EC 3.1.3.16)
(PP2C) (Abscisic acid- insensitive 1) {Arabidopsis
thaliana}
Length = 434
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 2/41 (4%)
Frame = -3
Query: 427 GSLVTYLDSLI--TDNTFLYEALTVPSLGYTKIFGSLPHLE 311
GS +T +I T++ L+E +VP G+T I G P +E
Sbjct: 102 GSDITSEKKMISRTESRSLFEFKSVPLYGFTSICGRRPEME 142
>At1g18780.1 68414.m02341 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 325
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/30 (40%), Positives = 14/30 (46%)
Frame = -3
Query: 382 FLYEALTVPSLGYTKIFGSLPHLEKSKHVS 293
F YE + LG +F LPHL H S
Sbjct: 188 FPYEEASSSQLGEVVLFHGLPHLRDEHHAS 217
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,232,993
Number of Sequences: 28952
Number of extensions: 226926
Number of successful extensions: 657
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 639
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 657
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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