Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30325
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    82   4e-17
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    81   1e-16
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    55   5e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    41   1e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    40   2e-04
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    27   1.7  
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c...    25   6.7  

>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 82.2 bits (194), Expect = 4e-17
 Identities = 45/122 (36%), Positives = 66/122 (54%), Gaps = 2/122 (1%)
 Frame = +3

Query: 156 SRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELD 335
           ++ AKR  S  F+  +  Q+ E KEAF L+D D DG IG+ D++    SL + ASE  ++
Sbjct: 28  AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87

Query: 336 EMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLRHALMTW 509
            M    + PIN    LT   + +   S  +D ++ AF TFD  + GKI    +R AL + 
Sbjct: 88  HMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSM 146

Query: 510 GD 515
           GD
Sbjct: 147 GD 148


>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 80.6 bits (190), Expect = 1e-16
 Identities = 44/111 (39%), Positives = 66/111 (59%), Gaps = 6/111 (5%)
 Frame = +3

Query: 201 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 368
           + +Q+AEF+EAF L D D+DG I  N+L     SLG+  +  EL +M+ E     +G I+
Sbjct: 7   TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66

Query: 369 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGD 515
           FT+ LT+ A +M   +D ++ V  AFK FD++G   I  E L H L + G+
Sbjct: 67  FTEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGE 116



 Score = 37.1 bits (82), Expect = 0.002
 Identities = 20/65 (30%), Positives = 36/65 (55%), Gaps = 4/65 (6%)
 Frame = +3

Query: 219 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLT 386
           E +EAF++ D D +G I   +L     SLG   S++E+ +M+ EA     G IN+ +   
Sbjct: 86  EVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSR 145

Query: 387 LFANR 401
           + +++
Sbjct: 146 VISSK 150


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 55.2 bits (127), Expect = 5e-09
 Identities = 32/107 (29%), Positives = 59/107 (55%), Gaps = 2/107 (1%)
 Frame = +3

Query: 201 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQL 380
           S++Q  E KEAF L D DKDG+I  + + +   SLG   ++ EL ++  E    I+  + 
Sbjct: 4   SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63

Query: 381 LTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGD 515
           ++  +N++     E++  I AF+ FD++  G I++ +    + T G+
Sbjct: 64  MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLGE 109


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 40.7 bits (91), Expect = 1e-04
 Identities = 18/42 (42%), Positives = 26/42 (61%)
 Frame = +3

Query: 219 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 344
           EF + FQ+ D D  G+IG  +LR    SLG   S +E+DE++
Sbjct: 78  EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 39.9 bits (89), Expect = 2e-04
 Identities = 29/103 (28%), Positives = 51/103 (49%), Gaps = 6/103 (5%)
 Frame = +3

Query: 201 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 368
           +++Q  +  EAF+L D DKD  I  ++LRA   +LG  A + E+ +++ +      G + 
Sbjct: 32  TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91

Query: 369 FTQLLTLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERLR 491
               + +   ++    D  + +  AF+ F  DE GKI    LR
Sbjct: 92  MEDFVRVMTEKIV-ERDPLEEIKRAFELFDDDETGKISLRNLR 133


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 27.1 bits (57), Expect = 1.7
 Identities = 15/42 (35%), Positives = 22/42 (52%)
 Frame = +3

Query: 219 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 344
           E +EAF L D    G I   DLR +   LG   ++++L  M+
Sbjct: 13  EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54


>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1274

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = +3

Query: 210 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASE 323
           + A+FK+ F     DKD +  + +L A  + L +LA +
Sbjct: 212 EYAKFKDKFPEAPLDKDNLQTQKELEARIECLKQLAEK 249


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.317    0.123    0.384 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,569,703
Number of Sequences: 5004
Number of extensions: 23716
Number of successful extensions: 104
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 103
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)