BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30323 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 75 5e-15 SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 63 2e-11 SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 36 0.005 SPAC1142.08 |fhl1|SPAC8C9.01|fork head transcription factor Fhl1... 28 0.95 SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos... 27 2.2 SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 2.9 SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces po... 26 2.9 SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi... 26 3.8 SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 6.7 SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharom... 25 6.7 SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|ch... 25 6.7 SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||M... 25 6.7 SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizo... 25 8.9 SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr 1|... 25 8.9 SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 25 8.9 SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 25 8.9 >SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein Stg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 174 Score = 75.4 bits (177), Expect = 5e-15 Identities = 54/146 (36%), Positives = 76/146 (52%), Gaps = 4/146 (2%) Frame = +2 Query: 74 EQEVLTWISDVLGEPLPNGAYE--DVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN 247 E+E WI + L L N + D L+ GVILC++ K A G+ + +E F MEN Sbjct: 6 EKEAREWIEETLHTKL-NAQLDLLDQLQSGVILCRIC-KEALGANIRYKESNMPFVQMEN 63 Query: 248 IQRF-QAAIKKYGVPEEEIFQTADPFERRNIPQVTLCLYALGRITQKHPEFTGPQLGPKM 424 I F A + VP +++FQT+D FERRN QV +++ R K LGPK+ Sbjct: 64 ISAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKL 123 Query: 425 ADKNERTFTEEQLRAHNAELN-LQMG 499 A+K R F+ +Q R +N LQ G Sbjct: 124 AEKKPRVFSAQQQREFREGVNSLQYG 149 >SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual Length = 1489 Score = 63.3 bits (147), Expect = 2e-11 Identities = 35/98 (35%), Positives = 51/98 (52%), Gaps = 2/98 (2%) Frame = +2 Query: 80 EVLTWISDVLGEPL-PNGAYEDVLRDGVILCKLANKLAPGSVKKI-QERGTNFQLMENIQ 253 E WI + LG L P +E LR+GV+L L K P + KI F+ +NI Sbjct: 46 EAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNIN 105 Query: 254 RFQAAIKKYGVPEEEIFQTADPFERRNIPQVTLCLYAL 367 +F I G+PE F+ D +E +N+P+V C++AL Sbjct: 106 KFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143 >SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 962 Score = 35.5 bits (78), Expect = 0.005 Identities = 29/101 (28%), Positives = 44/101 (43%), Gaps = 5/101 (4%) Frame = +2 Query: 80 EVLTWISDVLGEPLPN-GAYEDVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN--- 247 E W+ + N + D L +G +LC+LA K P + R +Q+ E Sbjct: 69 EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYPKLASNWKPR---YQISERNTV 125 Query: 248 -IQRFQAAIKKYGVPEEEIFQTADPFERRNIPQVTLCLYAL 367 + F + G+ F+T D R NIP+V CL+AL Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166 >SPAC1142.08 |fhl1|SPAC8C9.01|fork head transcription factor Fhl1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 743 Score = 27.9 bits (59), Expect = 0.95 Identities = 19/89 (21%), Positives = 35/89 (39%) Frame = +2 Query: 173 LANKLAPGSVKKIQERGTNFQLMENIQRFQAAIKKYGVPEEEIFQTADPFERRNIPQVTL 352 +AN LA +K+Q++ T Q + +Q+ K Y + TA P P ++ Sbjct: 511 IANALAVALAQKLQKQQTQMQGPQQVQQQAKRRKAYTSQQLNPAPTAMPHPNITSPSPSI 570 Query: 353 CLYALGRITQKHPEFTGPQLGPKMADKNE 439 + + P + P K+ D + Sbjct: 571 SVTQRPAVNVGPPPYVRPSAPSKLPDTRQ 599 >SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 26.6 bits (56), Expect = 2.2 Identities = 14/41 (34%), Positives = 22/41 (53%) Frame = -1 Query: 243 SMSWKLVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFG 121 S+++KL + DP LAN +++ SLS +Y P G Sbjct: 33 SLAYKLYSMLRSLKLDPHFMELANPKKVDSSLSYENYQPIG 73 >SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 163 Score = 26.2 bits (55), Expect = 2.9 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +1 Query: 160 DPLQIGQQISSWISEKNPRERNQLPAHG 243 D LQ+ ++ SWI+EK E +L + G Sbjct: 11 DSLQVDEKAKSWIAEKVEEELRRLESVG 38 >SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces pombe|chr 3|||Manual Length = 452 Score = 26.2 bits (55), Expect = 2.9 Identities = 17/64 (26%), Positives = 27/64 (42%) Frame = -1 Query: 228 LVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFGSGSPKTSEIQVRTSCSWSLFLAGI 49 L+ ++W +P + +Q++ PS+ T A P E + FL GI Sbjct: 257 LMHMAWALLDNPNLFVEPYVQQLMPSILTCLVAKRLGSDPNNHEHYALRDL--AAFLLGI 314 Query: 48 VCRR 37 VC R Sbjct: 315 VCDR 318 >SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schizosaccharomyces pombe|chr 2|||Manual Length = 749 Score = 25.8 bits (54), Expect = 3.8 Identities = 11/30 (36%), Positives = 15/30 (50%) Frame = -1 Query: 180 LANLQRITPSLSTSSYAPFGSGSPKTSEIQ 91 L+N +TPS S S Y P +T +Q Sbjct: 689 LSNFNSVTPSSSASLYPPLIPSEARTPSVQ 718 >SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 780 Score = 25.0 bits (52), Expect = 6.7 Identities = 11/39 (28%), Positives = 23/39 (58%) Frame = -1 Query: 180 LANLQRITPSLSTSSYAPFGSGSPKTSEIQVRTSCSWSL 64 L++ I+ L ++ +PF S S +E++ RTS ++ + Sbjct: 26 LSSYTSISRELDYANQSPFSSNSFSPTELKARTSATFDV 64 >SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 787 Score = 25.0 bits (52), Expect = 6.7 Identities = 12/41 (29%), Positives = 21/41 (51%) Frame = +3 Query: 372 ELLKNIPSLQDLS*DLKWLIRTNVPSRKNNFGPTTLNLTSK 494 EL+ N PS D + L + + VP+ + FG + + +K Sbjct: 564 ELIPNDPSDDDYAAALNYAVNEYVPTYRKTFGNDSKKIKNK 604 >SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 701 Score = 25.0 bits (52), Expect = 6.7 Identities = 19/68 (27%), Positives = 34/68 (50%) Frame = -3 Query: 451 REGTFVLISHFRS*LRSCKLGMFLSNSSQSVQT*CYLGNVTPLKRIGGLEYFFFWYSVFL 272 +EGT L + ++S +++ +S V + N T +K +GGLE F + L Sbjct: 376 KEGTEKLFALMEE-IKSVNDNRYITETSNKV-----MENFTEIKNLGGLE-FLRSEASSL 428 Query: 271 DCRLEPLN 248 DC ++ +N Sbjct: 429 DCLIDKVN 436 >SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||Manual Length = 535 Score = 25.0 bits (52), Expect = 6.7 Identities = 13/25 (52%), Positives = 15/25 (60%) Frame = -3 Query: 274 LDCRLEPLNVLHELEVGSSLLDFFH 200 LD LEPL LHE S+ L FF+ Sbjct: 238 LDAYLEPLVHLHEYSRRSNTLFFFY 262 >SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizosaccharomyces pombe|chr 2|||Manual Length = 778 Score = 24.6 bits (51), Expect = 8.9 Identities = 13/43 (30%), Positives = 22/43 (51%) Frame = -1 Query: 303 NISSSGTPYFLIAAWNL*MFSMSWKLVPLSWIFFTDPGANLLA 175 N +G PY I AW + S+ + P+ ++ + G +LLA Sbjct: 136 NYKDNGVPYVTIRAWPALLSSL---VPPVVFLIMKESGYDLLA 175 >SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr 1|||Manual Length = 623 Score = 24.6 bits (51), Expect = 8.9 Identities = 8/18 (44%), Positives = 11/18 (61%) Frame = -1 Query: 354 HNVTWGMLRLSKGSAVWN 301 HN WG R + +A+WN Sbjct: 491 HNFVWGRFRELEHAAMWN 508 >SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 2052 Score = 24.6 bits (51), Expect = 8.9 Identities = 11/27 (40%), Positives = 13/27 (48%) Frame = +1 Query: 154 RCDPLQIGQQISSWISEKNPRERNQLP 234 RC P Q G S W +E P + LP Sbjct: 1617 RCSPKQSGATRSVWFNELGPLTLSFLP 1643 >SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1953 Score = 24.6 bits (51), Expect = 8.9 Identities = 11/26 (42%), Positives = 16/26 (61%) Frame = -1 Query: 105 TSEIQVRTSCSWSLFLAGIVCRRNLV 28 T I V+TSCSW L +C +N++ Sbjct: 638 TKFISVQTSCSWITGLTLRLCFQNVL 663 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,386,848 Number of Sequences: 5004 Number of extensions: 52488 Number of successful extensions: 178 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 172 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 177 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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