BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30316 (516 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g07671.1 68415.m00894 H+-transporting two-sector ATPase, C su... 44 8e-05 At4g11170.1 68417.m01809 disease resistance protein (TIR-NBS-LRR... 28 4.3 At1g67120.1 68414.m07636 midasin-related similar to Midasin (MID... 28 4.3 At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative / th... 28 4.3 At5g16780.1 68418.m01965 SART-1 family protein contains Pfam dom... 27 5.7 At5g11040.1 68418.m01290 expressed protein weak similarity to hy... 27 7.5 At4g08450.1 68417.m01393 disease resistance protein (TIR-NBS-LRR... 27 7.5 At3g48380.2 68416.m05281 expressed protein 27 7.5 At3g48380.1 68416.m05280 expressed protein 27 7.5 At3g18215.1 68416.m02317 expressed protein contains Pfam profile... 27 7.5 At5g41460.1 68418.m05035 fringe-related protein strong similarit... 27 9.9 At5g35210.2 68418.m04175 peptidase M50 family protein / sterol-r... 27 9.9 At5g35210.1 68418.m04174 peptidase M50 family protein / sterol-r... 27 9.9 At5g21120.1 68418.m02518 ethylene-insensitive3-like2 (EIL2) iden... 27 9.9 At5g20220.1 68418.m02407 zinc knuckle (CCHC-type) family protein... 27 9.9 At4g26530.1 68417.m03822 fructose-bisphosphate aldolase, putativ... 27 9.9 At3g61290.1 68416.m06859 hypothetical protein 27 9.9 At3g56450.1 68416.m06278 alpha-soluble NSF attachment protein 1 ... 27 9.9 At3g27330.1 68416.m03418 zinc finger (C3HC4-type RING finger) fa... 27 9.9 At3g13050.1 68416.m01626 transporter-related low similarity to a... 27 9.9 >At2g07671.1 68415.m00894 H+-transporting two-sector ATPase, C subunit family protein similar to ATPase subunit 9 [Arabidopsis thaliana] GI:15215920; contains Pfam profile PF00137: ATP synthase subunit C Length = 85 Score = 43.6 bits (98), Expect = 8e-05 Identities = 21/30 (70%), Positives = 23/30 (76%) Frame = +1 Query: 295 FGSLIIGYARNPSLKQQLFSYAILGFALSE 384 F SLI ARNPSL +Q F YAILGFAL+E Sbjct: 39 FSSLIHSVARNPSLAKQSFGYAILGFALTE 68 >At4g11170.1 68417.m01809 disease resistance protein (TIR-NBS-LRR class), putative domain signature TIR-NBS-LRR exists, suggestive of a disease resistance protein. Length = 1095 Score = 27.9 bits (59), Expect = 4.3 Identities = 14/39 (35%), Positives = 19/39 (48%), Gaps = 2/39 (5%) Frame = +2 Query: 29 LIKTKCCLPPD*SPLQPGLPSSATLH--WCDHLQQYPPI 139 L++ CC + P LPS LH +C LQ +P I Sbjct: 682 LLEMSCCKKLEIIPTNINLPSLEVLHFRYCTRLQTFPEI 720 >At1g67120.1 68414.m07636 midasin-related similar to Midasin (MIDAS-containing protein) (Swiss-Prot:Q12019) [Saccharomyces cerevisiae]; similar to Midasin (MIDAS-containing protein) (Swiss-Prot:Q9NU22) [Homo sapiens]; contains Prosite PS00017: ATP/GTP-binding site motif A (P-loop) Length = 5336 Score = 27.9 bits (59), Expect = 4.3 Identities = 13/43 (30%), Positives = 24/43 (55%) Frame = -2 Query: 233 NLAAESMSLVTDVVWKDRTAESCVGTAGTICVWVGTAASGRTS 105 N+A+E + L+ ++ CV +C+ VG ++SG+TS Sbjct: 2028 NIASEQLKLLPEIRQNLEAVAHCVQNKW-LCILVGPSSSGKTS 2069 >At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative similar to SP|O04630 Threonyl-tRNA synthetase, mitochondrial precursor (EC 6.1.1.3) (Threonine--tRNA ligase) (ThrRS) {Arabidopsis thaliana}; contains Pfam profiles PF00587: tRNA synthetase class II core domain (G, H, P, S and T), PF03129: Anticodon binding domain, PF02824: TGS domain Length = 458 Score = 27.9 bits (59), Expect = 4.3 Identities = 14/44 (31%), Positives = 26/44 (59%) Frame = +2 Query: 188 SRPHRSLRTLTLLPNSLVLVQRQWE*LVPELVLEQSSAPSSSAM 319 SRPH ++ +TLLP+ + R+WE ++ ++ S + SA+ Sbjct: 37 SRPHDPIK-ITLLPDGIEKEGRRWETSPMDIAVQISKGLAKSAL 79 >At5g16780.1 68418.m01965 SART-1 family protein contains Pfam domain, PF03343: SART-1 family Length = 820 Score = 27.5 bits (58), Expect = 5.7 Identities = 11/32 (34%), Positives = 17/32 (53%) Frame = -1 Query: 321 GIADDEGAEDCSNTSSGTSYSHCRCTSTNEFG 226 GI DD+G ++ + S + R T+EFG Sbjct: 660 GIVDDDGGKESKDKESKDRFKDIRIERTDEFG 691 >At5g11040.1 68418.m01290 expressed protein weak similarity to hypercellular protein [Aspergillus nidulans] GI:9309269 Length = 1186 Score = 27.1 bits (57), Expect = 7.5 Identities = 11/29 (37%), Positives = 15/29 (51%) Frame = -1 Query: 216 NVLSDRCGLEGPHCRELCRDSRYHLCMGG 130 NVL R G P+ CR +H+C+ G Sbjct: 1155 NVLRARAGTASPNEPIFCRGPPFHVCVAG 1183 >At4g08450.1 68417.m01393 disease resistance protein (TIR-NBS-LRR class), putative domain signature TIR-NBS-LRR exists, suggestive of a disease resistance protein. Length = 1234 Score = 27.1 bits (57), Expect = 7.5 Identities = 12/38 (31%), Positives = 21/38 (55%), Gaps = 1/38 (2%) Frame = -1 Query: 312 DDEGAEDCSNTSSGTSYS-HCRCTSTNEFGSRVNVLSD 202 DDE ++D + SS T YS C + ++ G+ ++ D Sbjct: 1108 DDESSDDDDDLSSETDYSDECEDSDDSDLGNEIDYSED 1145 >At3g48380.2 68416.m05281 expressed protein Length = 640 Score = 27.1 bits (57), Expect = 7.5 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = +3 Query: 264 SWFRSWYWNSLRLPHHRLCQEPL 332 SWFR ++ S+ +P HR Q+ L Sbjct: 477 SWFRLQHYTSISVPSHREIQQTL 499 >At3g48380.1 68416.m05280 expressed protein Length = 645 Score = 27.1 bits (57), Expect = 7.5 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = +3 Query: 264 SWFRSWYWNSLRLPHHRLCQEPL 332 SWFR ++ S+ +P HR Q+ L Sbjct: 482 SWFRLQHYTSISVPSHREIQQTL 504 >At3g18215.1 68416.m02317 expressed protein contains Pfam profile PF04654: Protein of unknown function, DUF599 Length = 244 Score = 27.1 bits (57), Expect = 7.5 Identities = 16/52 (30%), Positives = 23/52 (44%), Gaps = 1/52 (1%) Frame = +2 Query: 362 FWVSPCLRLWVCSVL*WRSCCSSLSKLFTFKNTTTAIL-HMRFQCILSGVDS 514 F+ S L LW + CC +S + F +TTT+ H+ Q DS Sbjct: 181 FYFSFPLFLWTFGPIPMFVCCCMMSSILYFLDTTTSFTRHLHSQSFRETADS 232 >At5g41460.1 68418.m05035 fringe-related protein strong similarity to unknown protein (pir||T13026) similarity to predicted proteins + similar to hypothetical protein GB:AAC23643 [Arabidopsis thaliana] + weak similarity to Fringe [Schistocerca gregaria](GI:6573138);Fringe encodes an extracellular protein that regulates Notch signalling. Length = 524 Score = 26.6 bits (56), Expect = 9.9 Identities = 11/37 (29%), Positives = 17/37 (45%) Frame = +2 Query: 281 VLEQSSAPSSSAMPGTPPXXXXXXHTPFWVSPCLRLW 391 V++ ++P S P PP H F ++ RLW Sbjct: 90 VIKSYASPPPSPPPPPPPPQTGFQHVVFGIAASARLW 126 >At5g35210.2 68418.m04175 peptidase M50 family protein / sterol-regulatory element binding protein (SREBP) site 2 protease family protein contains PFam PF02163: sterol-regulatory element binding protein (SREBP) site 2 protease Length = 1409 Score = 26.6 bits (56), Expect = 9.9 Identities = 8/17 (47%), Positives = 9/17 (52%) Frame = -1 Query: 123 CKWSHQCRVAEDGRPGC 73 C W H CR+ R GC Sbjct: 724 CGWCHSCRLTSASRRGC 740 >At5g35210.1 68418.m04174 peptidase M50 family protein / sterol-regulatory element binding protein (SREBP) site 2 protease family protein contains PFam PF02163: sterol-regulatory element binding protein (SREBP) site 2 protease Length = 1576 Score = 26.6 bits (56), Expect = 9.9 Identities = 8/17 (47%), Positives = 9/17 (52%) Frame = -1 Query: 123 CKWSHQCRVAEDGRPGC 73 C W H CR+ R GC Sbjct: 724 CGWCHSCRLTSASRRGC 740 >At5g21120.1 68418.m02518 ethylene-insensitive3-like2 (EIL2) identical to ethylene-insensitive3-like2 (EIL2) GI:2224929 from [Arabidopsis thaliana] Length = 518 Score = 26.6 bits (56), Expect = 9.9 Identities = 12/27 (44%), Positives = 16/27 (59%), Gaps = 2/27 (7%) Frame = +3 Query: 120 CSSTHPYTDGTCC--PYTALCSAVLPD 194 CSS P+T+G C +TALC + D Sbjct: 16 CSSAPPFTEGHMCSDSHTALCDDLSSD 42 >At5g20220.1 68418.m02407 zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle Length = 393 Score = 26.6 bits (56), Expect = 9.9 Identities = 14/37 (37%), Positives = 17/37 (45%), Gaps = 3/37 (8%) Frame = -1 Query: 156 SRYHLCMGGYCCKWSHQ---CRVAEDGRPGCRGDQSG 55 +RYH C G C + H CR P C G+ SG Sbjct: 318 TRYHKC--GICGERGHNSRTCRKPTGVNPSCSGENSG 352 >At4g26530.1 68417.m03822 fructose-bisphosphate aldolase, putative strong similarity to SP|P22197 Fructose-bisphosphate aldolase, cytoplasmic isozyme (EC 4.1.2.13) {Arabidopsis thaliana} Length = 358 Score = 26.6 bits (56), Expect = 9.9 Identities = 13/29 (44%), Positives = 19/29 (65%), Gaps = 1/29 (3%) Frame = -1 Query: 348 QLLLEGGV-PGIADDEGAEDCSNTSSGTS 265 +LL+E GV PGI D+G D + T+ T+ Sbjct: 91 ELLMENGVIPGIKVDKGVVDLAGTNGETT 119 >At3g61290.1 68416.m06859 hypothetical protein Length = 455 Score = 26.6 bits (56), Expect = 9.9 Identities = 14/51 (27%), Positives = 24/51 (47%), Gaps = 1/51 (1%) Frame = -3 Query: 157 QQVPSVYGWVLLQVVAPVQSCRRWQTWL-QGRSVWRQTAFCFYKVRRATTK 8 Q PS Y L +++ +++C + W+ Q VW +T + RA K Sbjct: 24 QTCPSTYPSRLEPMISSLETCPDYFRWIQQDLKVWEETGITRETLERAKPK 74 >At3g56450.1 68416.m06278 alpha-soluble NSF attachment protein 1 / alpha-SNAP1 (ASNAP1) identical to alpha-soluble NSF attachment protein 1 (Alpha-SNAP1) (N- ethylmaleimide-sensitive factor attachment protein, alpha 1) (Swiss-Prot:Q9LXZ5) [Arabidopsis thaliana] Length = 381 Score = 26.6 bits (56), Expect = 9.9 Identities = 18/42 (42%), Positives = 21/42 (50%), Gaps = 1/42 (2%) Frame = -1 Query: 453 LKVNSLESE-EQQERHHKTEQTHSLRQGETQNGV*EQLLLEG 331 LKV S+ EQQ R KT+ HSL + GV LL G Sbjct: 210 LKVAQYASQLEQQSRFMKTQARHSLNNKLLKYGVKGHLLTAG 251 >At3g27330.1 68416.m03418 zinc finger (C3HC4-type RING finger) family protein contains Pfam profiles: PF00097 zinc finger, C3HC4 type (RING finger), PF01697 Domain of unknown function Length = 913 Score = 26.6 bits (56), Expect = 9.9 Identities = 9/20 (45%), Positives = 14/20 (70%) Frame = -1 Query: 207 SDRCGLEGPHCRELCRDSRY 148 +D+CG + P CR+L + RY Sbjct: 752 ADKCGRKCPKCRQLIGNGRY 771 >At3g13050.1 68416.m01626 transporter-related low similarity to apical organic cation transporter [Sus scrofa] GI:2062135, SP|Q02563 Synaptic vesicle protein 2 (SV2) {Rattus norvegicus}; contains Pfam profile PF00083: major facilitator superfamily protein Length = 500 Score = 26.6 bits (56), Expect = 9.9 Identities = 11/30 (36%), Positives = 19/30 (63%) Frame = -1 Query: 444 NSLESEEQQERHHKTEQTHSLRQGETQNGV 355 + LE+E ++ ++ TE TH L+ GE+ V Sbjct: 236 SELETELEENKNIPTENTHLLKAGESGEAV 265 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,520,560 Number of Sequences: 28952 Number of extensions: 243458 Number of successful extensions: 795 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 762 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 794 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 937669760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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