Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30309
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom...   168   5e-43
SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom...   136   1e-33
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ...    73   3e-14
SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase Le...    37   0.002
SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pomb...    27   2.2  
SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|...    25   5.1  
SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces pomb...    25   6.7  
SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10 |Schizo...    25   8.9  
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces...    25   8.9  

>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score =  168 bits (408), Expect = 5e-43
 Identities = 84/148 (56%), Positives = 105/148 (70%), Gaps = 5/148 (3%)
 Frame = +3

Query: 84  ATRAGAAQY-----STGVRKVTLIPGHGIGPEITVAVQKIFEAAKVPIEWEEVDVTAVRG 248
           +T+A A  Y     + G   VT+I G GIGPEI  +V++IF+AAKVPIEWE V V  +  
Sbjct: 28  STKAAAGTYEGVKNANGNYTVTMIAGDGIGPEIAQSVERIFKAAKVPIEWERVKVYPIL- 86

Query: 249 PDGKFGIPQKAIDSVNANKIGLKGPLMTPVGKGYRSLNLALRKEFDLYANVRPCKSLEGI 428
            +G   IP  A +SV  NK+ LKGPL TP+GKG+ S+NL LR+ F L+ANVRPC S+ G 
Sbjct: 87  KNGTTTIPDDAKESVRKNKVALKGPLATPIGKGHVSMNLTLRRTFGLFANVRPCVSITGY 146

Query: 429 KTLYDNVDVVTIRENTEGEYSGIEHEIV 512
           KT YDNV+ V IRENTEGEYSGIEHE++
Sbjct: 147 KTPYDNVNTVLIRENTEGEYSGIEHEVI 174


>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 356

 Score =  136 bits (330), Expect = 1e-33
 Identities = 72/134 (53%), Positives = 92/134 (68%), Gaps = 2/134 (1%)
 Frame = +3

Query: 117 GVRKVTLIPGHGIGPEITVAVQKIFEAAKVPIEWEEVDVTAVRGPDGKFGIP-QKAIDSV 293
           G   VTLIPG GIG E + AV +IF+ A VPIE+EE+DVT +   +   G    +AI S+
Sbjct: 19  GKYTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSSGDALHEAIQSL 78

Query: 294 NANKIGLKGPLMTPVGKG-YRSLNLALRKEFDLYANVRPCKSLEGIKTLYDNVDVVTIRE 470
             NK+GLKG L TP  KG + S N+ALRKE D+YA++   K++ G KT +DNVD   IRE
Sbjct: 79  KRNKVGLKGILFTPFEKGGHTSFNVALRKELDIYASLVLIKNIPGFKTRHDNVDFAIIRE 138

Query: 471 NTEGEYSGIEHEIV 512
           NTEGEYSG+EH+ V
Sbjct: 139 NTEGEYSGLEHQSV 152


>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 362

 Score = 72.5 bits (170), Expect = 3e-14
 Identities = 48/125 (38%), Positives = 72/125 (57%), Gaps = 7/125 (5%)
 Frame = +3

Query: 135 LIPGHGIGPEITVAVQKIFE--AAKVPIEWEEVDVTAVRGPDGKFG--IPQKAIDSVNAN 302
           LIP  GIG E+  A +++ E   AK  ++++ +D+ A  G   + G  +P++ ++ +   
Sbjct: 11  LIPADGIGKEVVPAARRLMENLPAKHKLKFDFIDLDAGWGTFERTGKALPERTVERLKTE 70

Query: 303 -KIGLKGPLMTPVGK--GYRSLNLALRKEFDLYANVRPCKSLEGIKTLYDNVDVVTIREN 473
               L G + +P  K  GY S  +ALRK+  LYANVRP KSL+G K     VD+V +REN
Sbjct: 71  CNAALFGAVQSPTHKVAGYSSPIVALRKKMGLYANVRPVKSLDGAKG--KPVDLVIVREN 128

Query: 474 TEGEY 488
           TE  Y
Sbjct: 129 TECLY 133


>SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase
           Leu1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 371

 Score = 37.1 bits (82), Expect = 0.002
 Identities = 43/142 (30%), Positives = 64/142 (45%), Gaps = 18/142 (12%)
 Frame = +3

Query: 123 RKVTLIPGHGIGPEITVA---VQKIFEAAKVPIEWEEVDVTAVRGPDGKFGIP-----QK 278
           +K+ ++PG  IGPEI  +   V K+ E  +  ++ E  +          +G P      K
Sbjct: 4   KKIVVLPGDHIGPEIVASALEVLKVVEKKRPELKLEFEEHKIGGASIDAYGTPLTDETVK 63

Query: 279 AIDSVNANKIG-LKGPLMT-PVGKGYRSLNLALRKEFDLYANVRPC----KSLEGIKTL- 437
           A    +   +G + GP  T P  +  + L L LRK   ++AN+RPC    KSL     L 
Sbjct: 64  ACLEADGVLLGAVGGPEWTNPNCRPEQGL-LKLRKSMGVWANLRPCNFASKSLVKYSPLK 122

Query: 438 ---YDNVDVVTIRENTEGEYSG 494
               + VD   +RE T G Y G
Sbjct: 123 PEIVEGVDFCVVRELTGGCYFG 144


>SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 713

 Score = 26.6 bits (56), Expect = 2.2
 Identities = 17/57 (29%), Positives = 29/57 (50%)
 Frame = -3

Query: 496 IPEYSPSVFSLIVTTSTLSYRVLIPSKLLQGLTLAYKSNSFLRAKLSDL*PLPTGVI 326
           IPEYS    + +  T+   + +  P+K + G  +   +N +L  K++    LPTG I
Sbjct: 516 IPEYSKLNVTAVNLTNHSYWNLASPNKTIDGTIIKSTTNVYL--KVNSETSLPTGDI 570


>SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 927

 Score = 25.4 bits (53), Expect = 5.1
 Identities = 11/26 (42%), Positives = 14/26 (53%)
 Frame = -2

Query: 167 DLGPNTMSRDERNLAHASAVLSSAGS 90
           DL PN   +++RNL    A L   GS
Sbjct: 2   DLKPNIRRKEKRNLLKGEAALEKKGS 27


>SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 955

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 16/46 (34%), Positives = 26/46 (56%), Gaps = 2/46 (4%)
 Frame = +3

Query: 333 PVGKGYRSLN--LALRKEFDLYANVRPCKSLEGIKTLYDNVDVVTI 464
           PV   Y+S++  LA   +  L A V+   +   ++ L DN+D+VTI
Sbjct: 584 PVANVYQSVHSFLAPSLQSILLAQVKLNPTQAELEALADNIDIVTI 629


>SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 395

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 12/32 (37%), Positives = 17/32 (53%)
 Frame = +3

Query: 231 VTAVRGPDGKFGIPQKAIDSVNANKIGLKGPL 326
           V+AVR P G FG    ++ +     I +KG L
Sbjct: 9   VSAVRTPMGSFGGSFASLPATKLGSIAIKGAL 40


>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1485

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = -2

Query: 221 LPFDRYFSCFEDFLNGDRDLGPNTMSR 141
           +  D  FS +ED  +GD ++ PNT ++
Sbjct: 1   MSIDADFSDYEDEASGDENVLPNTTTK 27


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,116,751
Number of Sequences: 5004
Number of extensions: 41891
Number of successful extensions: 123
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 117
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)