Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30307
(502 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 31 0.097
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 31 0.097
SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 27 2.1
SPCC830.08c |||Golgi membrane protein |Schizosaccharomyces pombe... 26 3.7
SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 26 3.7
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 25 4.8
SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 25 6.4
SPCC1620.10 |cwf26||complexed with Cdc5 protein Cwf26 |Schizosac... 25 8.4
>SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 527
Score = 31.1 bits (67), Expect = 0.097
Identities = 23/92 (25%), Positives = 44/92 (47%), Gaps = 4/92 (4%)
Frame = +3
Query: 138 ELEIALDHANKANAEAQKNIKRYQAQIKDLQTALXXXXXXXXXXXXXLGI-SERRANALQ 314
E ++ L NAE++K+ R + +++DL ++L + SE+R N L+
Sbjct: 87 EEKLQLTETKCRNAESEKS--RVENELEDLTSSLFEEANRMVANARKETVASEKRVNQLK 144
Query: 315 NELEESRTLLEQADRARRQAE---QELSDAHE 401
+L ++ TLL + + +SD+HE
Sbjct: 145 KQLVDAETLLSSTQHQLTELKDVMHSMSDSHE 176
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 31.1 bits (67), Expect = 0.097
Identities = 27/93 (29%), Positives = 39/93 (41%), Gaps = 1/93 (1%)
Frame = +3
Query: 114 KKLEADINELEIALDHANKANAEAQKN-IKRYQAQIKDLQTALXXXXXXXXXXXXXLGIS 290
KKLE D+N L L+ N A+ E+ KN I+ + K L L L
Sbjct: 753 KKLENDLNLLTEKLNKKN-ADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEE 811
Query: 291 ERRANALQNELEESRTLLEQADRARRQAEQELS 389
R LQ++L + + +E + A ELS
Sbjct: 812 STRLQELQSQLNQDKNQIETLNERISAAADELS 844
>SPCC132.04c |||NAD-dependent glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1106
Score = 26.6 bits (56), Expect = 2.1
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3
Query: 117 KLEADINELEIALDHANKANAEAQKNIKR 203
KL + + L LDH+N +AE + IKR
Sbjct: 430 KLGPEYSSLSAILDHSNNIHAEILETIKR 458
>SPCC830.08c |||Golgi membrane protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 182
Score = 25.8 bits (54), Expect = 3.7
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = +1
Query: 205 TRHKSRICRPLSRRSSAPVTMPANSSASRSVA 300
T H RIC+ +SR+++AP P SS + + A
Sbjct: 146 TPHVIRICKSVSRQNAAPA--PTASSFAHTTA 175
>SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit
Psm3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1194
Score = 25.8 bits (54), Expect = 3.7
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = +3
Query: 156 DHANKANAEAQKNIKRYQAQIKDLQTAL 239
D+ N + +A KN+K YQ + DLQ +L
Sbjct: 661 DYRN-SRLDAIKNVKTYQIKFSDLQESL 687
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 25.4 bits (53), Expect = 4.8
Identities = 18/101 (17%), Positives = 43/101 (42%), Gaps = 7/101 (6%)
Frame = +3
Query: 105 RMKKKLEADINELEIALDHANKANAE-------AQKNIKRYQAQIKDLQTALXXXXXXXX 263
+M K++ + + E +L HA AE K IK ++ +DL+ L
Sbjct: 420 KMVKQVSSQLEEARSSLAHATGKLAEINSERDFQNKKIKDFEKIEQDLRACLNSSSNELK 479
Query: 264 XXXXXLGISERRANALQNELEESRTLLEQADRARRQAEQEL 386
+ ++ N L+ +++E + + E + + ++++
Sbjct: 480 EKSALIDKKDQELNNLREQIKEQKKVSESTQSSLQSLQRDI 520
>SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2104
Score = 25.0 bits (52), Expect = 6.4
Identities = 18/74 (24%), Positives = 31/74 (41%)
Frame = +3
Query: 180 EAQKNIKRYQAQIKDLQTALXXXXXXXXXXXXXLGISERRANALQNELEESRTLLEQADR 359
+ + ++R A I +LQ L L I++ R L N+L+E + L + D
Sbjct: 833 QTDEYLRRKDALINNLQNQLESTKEVANE----LTITKERVLQLTNDLQEEQALAHEKDI 888
Query: 360 ARRQAEQELSDAHE 401
+A + HE
Sbjct: 889 LVERANSRVEVVHE 902
>SPCC1620.10 |cwf26||complexed with Cdc5 protein Cwf26
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 305
Score = 24.6 bits (51), Expect = 8.4
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = +3
Query: 294 RRANALQNELEESRTLLEQADRARRQAEQE 383
RR + + E R L E+ + ARRQ EQ+
Sbjct: 163 RRIDLVLARKEAKRKLKEKEEEARRQKEQQ 192
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.315 0.122 0.385
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,286,140
Number of Sequences: 5004
Number of extensions: 16760
Number of successful extensions: 87
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 84
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 87
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 198176188
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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