BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30304 (326 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p... 38 0.001 At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p... 37 0.003 At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 33 0.060 At5g32482.1 68418.m03835 zinc knuckle (CCHC-type) family protein... 27 3.9 >At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, putative similar to dihydrolipoamide S-acetyltransferase GI:5669871 [Zea mays]; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme, PF02817: e3 binding domain Length = 539 Score = 38.3 bits (85), Expect = 0.001 Identities = 16/31 (51%), Positives = 23/31 (74%) Frame = +1 Query: 223 FVDLPLSGMRETIAKRLTVAKQSIPHYQLSV 315 +VD+P S +R+ A RL +KQ+IPHY L+V Sbjct: 309 YVDIPHSQIRKVTASRLAFSKQTIPHYYLTV 339 Score = 25.8 bits (54), Expect = 6.8 Identities = 10/17 (58%), Positives = 15/17 (88%) Frame = +1 Query: 82 RVYASPMARRLAEIKHI 132 RV+ASP+AR+LAE ++ Sbjct: 246 RVFASPLARKLAEDNNV 262 >At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, putative similar to dihydrolipoamide S-acetyltransferase [Zea mays] GI:5669871; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme, PF02817: e3 binding domain Length = 539 Score = 37.1 bits (82), Expect = 0.003 Identities = 15/31 (48%), Positives = 23/31 (74%) Frame = +1 Query: 223 FVDLPLSGMRETIAKRLTVAKQSIPHYQLSV 315 +VD+P + +R+ A RL +KQ+IPHY L+V Sbjct: 309 YVDIPHTQIRKVTASRLAFSKQTIPHYYLTV 339 Score = 25.4 bits (53), Expect = 9.0 Identities = 9/17 (52%), Positives = 15/17 (88%) Frame = +1 Query: 82 RVYASPMARRLAEIKHI 132 R++ASP+AR+LAE ++ Sbjct: 246 RIFASPLARKLAEDNNV 262 >At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, putative similar to dihydrolipoamide acetyltransferase (E2) subunit of PDC [Arabidopsis thaliana] GI:559395; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme, PF02817: e3 binding domain; supporting cDNA gi|5881964|gb|AF066080.1|AF066080 Length = 637 Score = 32.7 bits (71), Expect = 0.060 Identities = 16/33 (48%), Positives = 20/33 (60%) Frame = +1 Query: 223 FVDLPLSGMRETIAKRLTVAKQSIPHYQLSVTV 321 + D P S +R+ IAKRL +KQ IPH L V Sbjct: 407 YEDFPNSQIRKIIAKRLLESKQKIPHLYLQSDV 439 >At5g32482.1 68418.m03835 zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle Length = 341 Score = 26.6 bits (56), Expect = 3.9 Identities = 12/21 (57%), Positives = 12/21 (57%) Frame = -2 Query: 67 VRRGQPQGWDVGRPERGRGRG 5 V RG P G GR GRGRG Sbjct: 220 VHRGNPHGRGHGRGRGGRGRG 240 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,608,020 Number of Sequences: 28952 Number of extensions: 35049 Number of successful extensions: 128 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 123 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 127 length of database: 12,070,560 effective HSP length: 71 effective length of database: 10,014,968 effective search space used: 370553816 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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