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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30304
         (326 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p...    38   0.001
At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p...    37   0.003
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p...    33   0.060
At5g32482.1 68418.m03835 zinc knuckle (CCHC-type) family protein...    27   3.9  

>At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase,
           putative similar to dihydrolipoamide S-acetyltransferase
           GI:5669871 [Zea mays]; contains Pfam profiles PF00198:
           2-oxo acid dehydrogenases acyltransferase (catalytic
           domain), PF00364: Biotin-requiring enzyme, PF02817: e3
           binding domain
          Length = 539

 Score = 38.3 bits (85), Expect = 0.001
 Identities = 16/31 (51%), Positives = 23/31 (74%)
 Frame = +1

Query: 223 FVDLPLSGMRETIAKRLTVAKQSIPHYQLSV 315
           +VD+P S +R+  A RL  +KQ+IPHY L+V
Sbjct: 309 YVDIPHSQIRKVTASRLAFSKQTIPHYYLTV 339



 Score = 25.8 bits (54), Expect = 6.8
 Identities = 10/17 (58%), Positives = 15/17 (88%)
 Frame = +1

Query: 82  RVYASPMARRLAEIKHI 132
           RV+ASP+AR+LAE  ++
Sbjct: 246 RVFASPLARKLAEDNNV 262


>At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase,
           putative similar to dihydrolipoamide S-acetyltransferase
           [Zea mays] GI:5669871; contains Pfam profiles PF00198:
           2-oxo acid dehydrogenases acyltransferase (catalytic
           domain), PF00364: Biotin-requiring enzyme, PF02817: e3
           binding domain
          Length = 539

 Score = 37.1 bits (82), Expect = 0.003
 Identities = 15/31 (48%), Positives = 23/31 (74%)
 Frame = +1

Query: 223 FVDLPLSGMRETIAKRLTVAKQSIPHYQLSV 315
           +VD+P + +R+  A RL  +KQ+IPHY L+V
Sbjct: 309 YVDIPHTQIRKVTASRLAFSKQTIPHYYLTV 339



 Score = 25.4 bits (53), Expect = 9.0
 Identities = 9/17 (52%), Positives = 15/17 (88%)
 Frame = +1

Query: 82  RVYASPMARRLAEIKHI 132
           R++ASP+AR+LAE  ++
Sbjct: 246 RIFASPLARKLAEDNNV 262


>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
           putative similar to dihydrolipoamide acetyltransferase
           (E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
           contains Pfam profiles PF00198: 2-oxo acid
           dehydrogenases acyltransferase (catalytic domain),
           PF00364: Biotin-requiring enzyme, PF02817: e3 binding
           domain; supporting cDNA
           gi|5881964|gb|AF066080.1|AF066080
          Length = 637

 Score = 32.7 bits (71), Expect = 0.060
 Identities = 16/33 (48%), Positives = 20/33 (60%)
 Frame = +1

Query: 223 FVDLPLSGMRETIAKRLTVAKQSIPHYQLSVTV 321
           + D P S +R+ IAKRL  +KQ IPH  L   V
Sbjct: 407 YEDFPNSQIRKIIAKRLLESKQKIPHLYLQSDV 439


>At5g32482.1 68418.m03835 zinc knuckle (CCHC-type) family protein
           contains Pfam domain, PF00098: Zinc knuckle
          Length = 341

 Score = 26.6 bits (56), Expect = 3.9
 Identities = 12/21 (57%), Positives = 12/21 (57%)
 Frame = -2

Query: 67  VRRGQPQGWDVGRPERGRGRG 5
           V RG P G   GR   GRGRG
Sbjct: 220 VHRGNPHGRGHGRGRGGRGRG 240


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,608,020
Number of Sequences: 28952
Number of extensions: 35049
Number of successful extensions: 128
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 12,070,560
effective HSP length: 71
effective length of database: 10,014,968
effective search space used: 370553816
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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