Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30294
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 78 7e-16
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 65 5e-12
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 38 7e-04
SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos... 27 2.2
SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 2.9
SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces po... 26 2.9
SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi... 26 3.8
SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr 1|... 25 5.1
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 6.7
SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharom... 25 6.7
SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1||... 25 6.7
SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|ch... 25 6.7
SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||M... 25 6.7
SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizo... 25 8.9
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 25 8.9
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 25 8.9
>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
Stg1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 174
Score = 78.2 bits (184), Expect = 7e-16
Identities = 55/146 (37%), Positives = 77/146 (52%), Gaps = 4/146 (2%)
Frame = +1
Query: 70 EQEVLTWISDVLGEPLPNGAYE--DVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN 243
E+E WI + L L N + D L+ GVILC++ K A G+ + +E F MEN
Sbjct: 6 EKEAREWIEETLHTKL-NAQLDLLDQLQSGVILCRIC-KEALGANIRYKESNMPFVQMEN 63
Query: 244 IQRF-QAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYALGRITQKHPEFTGPQLGPKM 420
I F A + VP +++FQT+DLFERRN QV +++ R K LGPK+
Sbjct: 64 ISAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKL 123
Query: 421 ADKNERTFTEEQLRAHNAELN-LQMG 495
A+K R F+ +Q R +N LQ G
Sbjct: 124 AEKKPRVFSAQQQREFREGVNSLQYG 149
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 65.3 bits (152), Expect = 5e-12
Identities = 35/98 (35%), Positives = 52/98 (53%), Gaps = 2/98 (2%)
Frame = +1
Query: 76 EVLTWISDVLGEPL-PNGAYEDVLRDGVILCKLANKLAPGSVKKI-QERGTNFQLMENIQ 249
E WI + LG L P +E LR+GV+L L K P + KI F+ +NI
Sbjct: 46 EAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNIN 105
Query: 250 RFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 363
+F I G+PE F+ D++E +N+P+V C++AL
Sbjct: 106 KFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143
>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
2|||Manual
Length = 962
Score = 38.3 bits (85), Expect = 7e-04
Identities = 30/101 (29%), Positives = 45/101 (44%), Gaps = 5/101 (4%)
Frame = +1
Query: 76 EVLTWISDVLGEPLPN-GAYEDVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN--- 243
E W+ + N + D L +G +LC+LA K P + R +Q+ E
Sbjct: 69 EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYPKLASNWKPR---YQISERNTV 125
Query: 244 -IQRFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 363
+ F + G+ F+T DL R NIP+V CL+AL
Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166
>SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase
Ubp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 26.6 bits (56), Expect = 2.2
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -2
Query: 239 SMSWKLVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFG 117
S+++KL + DP LAN +++ SLS +Y P G
Sbjct: 33 SLAYKLYSMLRSLKLDPHFMELANPKKVDSSLSYENYQPIG 73
>SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 163
Score = 26.2 bits (55), Expect = 2.9
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +3
Query: 156 DPLQIGQQISSWISEKNPRERNQLPAHG 239
D LQ+ ++ SWI+EK E +L + G
Sbjct: 11 DSLQVDEKAKSWIAEKVEEELRRLESVG 38
>SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 452
Score = 26.2 bits (55), Expect = 2.9
Identities = 17/64 (26%), Positives = 27/64 (42%)
Frame = -2
Query: 224 LVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFGSGSPKTSEIQVRTSCSWSLFLAGI 45
L+ ++W +P + +Q++ PS+ T A P E + FL GI
Sbjct: 257 LMHMAWALLDNPNLFVEPYVQQLMPSILTCLVAKRLGSDPNNHEHYALRDL--AAFLLGI 314
Query: 44 VCRR 33
VC R
Sbjct: 315 VCDR 318
>SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 749
Score = 25.8 bits (54), Expect = 3.8
Identities = 11/30 (36%), Positives = 15/30 (50%)
Frame = -2
Query: 176 LANLQRITPSLSTSSYAPFGSGSPKTSEIQ 87
L+N +TPS S S Y P +T +Q
Sbjct: 689 LSNFNSVTPSSSASLYPPLIPSEARTPSVQ 718
>SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 623
Score = 25.4 bits (53), Expect = 5.1
Identities = 8/18 (44%), Positives = 11/18 (61%)
Frame = -2
Query: 350 HNVTWGMLRLSKRSAVWN 297
HN WG R + +A+WN
Sbjct: 491 HNFVWGRFRELEHAAMWN 508
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 25.0 bits (52), Expect = 6.7
Identities = 11/39 (28%), Positives = 23/39 (58%)
Frame = -2
Query: 176 LANLQRITPSLSTSSYAPFGSGSPKTSEIQVRTSCSWSL 60
L++ I+ L ++ +PF S S +E++ RTS ++ +
Sbjct: 26 LSSYTSISRELDYANQSPFSSNSFSPTELKARTSATFDV 64
>SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/41 (29%), Positives = 21/41 (51%)
Frame = +2
Query: 368 ELLKNIPSLQDLS*DLKWLIRTNVPSRKNNFGPTTLNLTSK 490
EL+ N PS D + L + + VP+ + FG + + +K
Sbjct: 564 ELIPNDPSDDDYAAALNYAVNEYVPTYRKTFGNDSKKIKNK 604
>SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1101
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +2
Query: 215 EEPTSSSWRTFKGSKRQSRNTEYQKKKYSKPPIF 316
E+P++S W+T GS+ ++ +KK S P F
Sbjct: 185 EDPSNSYWKTVIGSREMFEDS--RKKTSSPSPSF 216
>SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 701
Score = 25.0 bits (52), Expect = 6.7
Identities = 19/68 (27%), Positives = 34/68 (50%)
Frame = -1
Query: 447 REGTFVLISHFRS*LRSCKLGMFLSNSSQSVQT*CYLGNVTPLKKIGGLEYFFFWYSVFL 268
+EGT L + ++S +++ +S V + N T +K +GGLE F + L
Sbjct: 376 KEGTEKLFALMEE-IKSVNDNRYITETSNKV-----MENFTEIKNLGGLE-FLRSEASSL 428
Query: 267 DCRLEPLN 244
DC ++ +N
Sbjct: 429 DCLIDKVN 436
>SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 535
Score = 25.0 bits (52), Expect = 6.7
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = -1
Query: 270 LDCRLEPLNVLHELEVGSSLLDFFH 196
LD LEPL LHE S+ L FF+
Sbjct: 238 LDAYLEPLVHLHEYSRRSNTLFFFY 262
>SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase
Ogm4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 778
Score = 24.6 bits (51), Expect = 8.9
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = -2
Query: 299 NISSSGTPYFLIAAWNL*MFSMSWKLVPLSWIFFTDPGANLLA 171
N +G PY I AW + S+ + P+ ++ + G +LLA
Sbjct: 136 NYKDNGVPYVTIRAWPALLSSL---VPPVVFLIMKESGYDLLA 175
>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2052
Score = 24.6 bits (51), Expect = 8.9
Identities = 11/27 (40%), Positives = 13/27 (48%)
Frame = +3
Query: 150 RCDPLQIGQQISSWISEKNPRERNQLP 230
RC P Q G S W +E P + LP
Sbjct: 1617 RCSPKQSGATRSVWFNELGPLTLSFLP 1643
>SPBC1826.01c |mot1||TATA-binding protein associated factor
Mot1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1953
Score = 24.6 bits (51), Expect = 8.9
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -2
Query: 101 TSEIQVRTSCSWSLFLAGIVCRRNLV 24
T I V+TSCSW L +C +N++
Sbjct: 638 TKFISVQTSCSWITGLTLRLCFQNVL 663
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.134 0.387
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,388,244
Number of Sequences: 5004
Number of extensions: 52493
Number of successful extensions: 181
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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