BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30290 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1778.02 |rap1||telomere binding protein Rap1|Schizosaccharom... 29 0.55 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 27 2.2 SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|c... 26 2.9 SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr... 25 5.1 SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 25 6.7 SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyce... 25 8.9 >SPBC1778.02 |rap1||telomere binding protein Rap1|Schizosaccharomyces pombe|chr 2|||Manual Length = 693 Score = 28.7 bits (61), Expect = 0.55 Identities = 23/96 (23%), Positives = 43/96 (44%), Gaps = 7/96 (7%) Frame = +3 Query: 222 NGAIQREAKRLIVKNKR-------FERSQVLAFVVEWIDENKELEWESTFSTSRQHESFR 380 + ++ + + +V NKR F + + ++ V+++DE+ L + Q R Sbjct: 218 SASVSKSENKALVNNKRYSDSYFYFSKMRRISIDVDYVDEDLNL----INAYLSQFGKKR 273 Query: 381 HVTELFRHIKRYFSFVEHLHNFSHWHGVFFKYDKFI 488 + EL + R FS + H FS W +F + FI Sbjct: 274 SLNELCALLSRRFS---NRHTFSEWRALFMHFFPFI 306 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 26.6 bits (56), Expect = 2.2 Identities = 24/74 (32%), Positives = 31/74 (41%), Gaps = 1/74 (1%) Frame = +1 Query: 148 GNRSPSW*MKISLKYMLG-TLNNASTTGRSRGKPRGLLSRTKDSKGAKSLPLLSNG*TKT 324 GN PS+ K S Y G NN+ G PR SR + +SNG + Sbjct: 308 GNSHPSY-NKYS-HYQHGFNYNNSGNNRNESGHPRFRNSRRNYNNQGAYPTYMSNGRSAN 365 Query: 325 KSWNGNPPSVPLGS 366 +S NP +V GS Sbjct: 366 QSPRNNPQNVNNGS 379 >SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 26.2 bits (55), Expect = 2.9 Identities = 20/70 (28%), Positives = 27/70 (38%) Frame = +1 Query: 211 NASTTGRSRGKPRGLLSRTKDSKGAKSLPLLSNG*TKTKSWNGNPPSVPLGSMSLLGM*Q 390 +A TTG S GKP G + T KG + G + K + P S Sbjct: 264 SAPTTGFSFGKPAGQAASTATDKGTTTTSSAGTGFSFGKPATTEDTNKPTAPNSAFTKPA 323 Query: 391 NSSDISKGTF 420 S+ +K TF Sbjct: 324 TSTGDNKPTF 333 >SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 703 Score = 25.4 bits (53), Expect = 5.1 Identities = 11/29 (37%), Positives = 18/29 (62%) Frame = +3 Query: 105 NIRLCVIRRKIEFVREPFTLVVNENILEI 191 N+ C++ RKIE E T V N++ +E+ Sbjct: 488 NVMNCLLYRKIENFEEVLTWVYNKSHIEL 516 >SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 435 Score = 25.0 bits (52), Expect = 6.7 Identities = 16/44 (36%), Positives = 21/44 (47%), Gaps = 1/44 (2%) Frame = +3 Query: 36 FLHFLWILRMQNSSVY*NLI-FWNNIRLCVIRRKIEFVREPFTL 164 FL W+ Q + +LI WN I CV+ IE VR T+ Sbjct: 91 FLKSSWLPLKQKRVLTSSLIKLWNEIHDCVLFDAIEHVRSIATI 134 >SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyces pombe|chr 1|||Manual Length = 906 Score = 24.6 bits (51), Expect = 8.9 Identities = 8/20 (40%), Positives = 14/20 (70%) Frame = -2 Query: 443 IMKMLDEGKVPFDMSEEFCY 384 I K++D G++ FD + + CY Sbjct: 472 IRKLIDIGEIDFDQAFKICY 491 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,219,028 Number of Sequences: 5004 Number of extensions: 48565 Number of successful extensions: 107 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 104 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 107 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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