BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30289 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4C3.09 |||acetylglucosaminyltransferase|Schizosaccharomyces ... 58 6e-10 SPBC4C3.08 |mug136||acetylglucosaminyltransferase|Schizosaccharo... 48 8e-07 SPAC5H10.12c |||acetylglucosaminyltransferase|Schizosaccharomyce... 43 3e-05 SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 28 0.72 SPAC7D4.03c |||conserved fungal family|Schizosaccharomyces pombe... 27 1.7 SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyc... 26 2.9 SPAC25B8.15c |||wybutosine biosynthesis protein Tyw3|Schizosacch... 25 5.1 SPBC1861.06c |mug131||S. pombe specific UPF0300 family protein 4... 25 6.7 >SPBC4C3.09 |||acetylglucosaminyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 376 Score = 58.4 bits (135), Expect = 6e-10 Identities = 44/146 (30%), Positives = 72/146 (49%), Gaps = 8/146 (5%) Frame = +3 Query: 75 VTLATN--DSYGLGALVLAHSLRR---AGSVYPAVALITPTVSEAMRDRLRAVFSEVVTV 239 +TL T+ D Y VL + L+ S YP L+ V E +RLR +E++ V Sbjct: 81 LTLPTSEHDVYFNATRVLVYKLKHHPETKSKYPVHVLVMKGVDEWKIERLRLDGAEIIMV 140 Query: 240 DVLDSRDAAHLAL---LQRPELGITFTKIHCWNLTQYEKCVFLDADILVIQNCDELFERE 410 D + + D L + FTK+ + TQ++K LD+D+LV++N D++F+ Sbjct: 141 DQIKTEDLIESGLSIGMGSYRYQYMFTKLSVFEQTQFDKVCILDSDLLVLKNMDDIFDTP 200 Query: 411 ELSAAPDVGWPDCFNSGVFVFKPSNE 488 + +P PD F+ +F KP +E Sbjct: 201 YVYESP--AEPDMFSFPIFK-KPDDE 223 >SPBC4C3.08 |mug136||acetylglucosaminyltransferase|Schizosaccharomyc es pombe|chr 2|||Manual Length = 372 Score = 48.0 bits (109), Expect = 8e-07 Identities = 36/120 (30%), Positives = 59/120 (49%), Gaps = 7/120 (5%) Frame = +3 Query: 93 DSYGLGALVLAHSL---RRAGSVYPAVALITPTVSEAMRDRLRAVFSEVVTVDVLDSR-- 257 D Y +L H L + S YP V L + + D+L+ + V VD L + Sbjct: 80 DWYYNSTRLLVHRLVKFKPTKSKYPVVVLAMKGIDQWKLDQLQEDGAIVKVVDPLYAHEV 139 Query: 258 --DAAHLALLQRPELGITFTKIHCWNLTQYEKCVFLDADILVIQNCDELFEREELSAAPD 431 D +ALL + FTK+ + + +Y++ FLD+DIL I+ D++F+ +LS + D Sbjct: 140 VDDVNDIALLDS-RWSMMFTKLRVFEMYEYDRICFLDSDILPIKKMDKVFDVHQLSYSKD 198 Score = 28.7 bits (61), Expect = 0.55 Identities = 11/24 (45%), Positives = 17/24 (70%) Frame = +3 Query: 444 DCFNSGVFVFKPSNETFEKLIQFA 515 D FN+G+FVFKP +++L+ A Sbjct: 256 DYFNAGLFVFKPLKAHYKRLMALA 279 >SPAC5H10.12c |||acetylglucosaminyltransferase|Schizosaccharomyces pombe|chr 1|||Manual Length = 371 Score = 42.7 bits (96), Expect = 3e-05 Identities = 31/115 (26%), Positives = 54/115 (46%), Gaps = 8/115 (6%) Frame = +3 Query: 84 ATNDSYGLGAL-VLAHSLR---RAGSVYPAVALITPTVSEAMRDRLRAVFSEVVTVDVLD 251 +T + Y A VL H L+ S YP L V E +R R + V+ +D + Sbjct: 79 STEEDYYFNATRVLIHRLKYHPTTKSKYPIHILALRGVDEWKIERFRKDGASVIVIDPIA 138 Query: 252 SRDAAHLALLQRPELGIT----FTKIHCWNLTQYEKCVFLDADILVIQNCDELFE 404 S D + E+ F+K+ + Q++K +D+DIL+++N D++F+ Sbjct: 139 SSDIVYDTSSFSQEISARYEQMFSKLRIFEQIQFDKICVIDSDILIMKNIDDIFD 193 >SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual Length = 1489 Score = 28.3 bits (60), Expect = 0.72 Identities = 13/34 (38%), Positives = 21/34 (61%) Frame = +3 Query: 156 PAVALITPTVSEAMRDRLRAVFSEVVTVDVLDSR 257 P V + +P + D L A +++V+T+DVLD R Sbjct: 1438 PTVKVSSPRCELHLDDLLSAQYNKVLTLDVLDGR 1471 >SPAC7D4.03c |||conserved fungal family|Schizosaccharomyces pombe|chr 1|||Manual Length = 886 Score = 27.1 bits (57), Expect = 1.7 Identities = 18/80 (22%), Positives = 40/80 (50%), Gaps = 2/80 (2%) Frame = +3 Query: 144 GSVYPAVA-LITPTVSEAMRDRLR-AVFSEVVTVDVLDSRDAAHLALLQRPELGITFTKI 317 G + P +A L T VSE D+LR ++++ V++ + + + + + K Sbjct: 175 GFLLPHLASLTTKNVSEPELDKLRHSLYNWWVSILRRLQSNISTSERITYTKAILAIAKH 234 Query: 318 HCWNLTQYEKCVFLDADILV 377 HCWN ++ ++L+ +++ Sbjct: 235 HCWNKVEHSALLYLEHQLIL 254 >SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 457 Score = 26.2 bits (55), Expect = 2.9 Identities = 14/36 (38%), Positives = 21/36 (58%) Frame = +3 Query: 129 SLRRAGSVYPAVALITPTVSEAMRDRLRAVFSEVVT 236 +LR+A + P V + TV+E +RD V + VVT Sbjct: 112 NLRKALTSTPNVTVTEATVNELLRDETGEVITGVVT 147 >SPAC25B8.15c |||wybutosine biosynthesis protein Tyw3|Schizosaccharomyces pombe|chr 1|||Manual Length = 237 Score = 25.4 bits (53), Expect = 5.1 Identities = 10/22 (45%), Positives = 12/22 (54%) Frame = -3 Query: 493 KVSFEGLKTKTPELKQSGHPTS 428 K EGLK+ P+ GHP S Sbjct: 15 KEILEGLKSSVPDASPKGHPDS 36 >SPBC1861.06c |mug131||S. pombe specific UPF0300 family protein 4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 25.0 bits (52), Expect = 6.7 Identities = 10/27 (37%), Positives = 17/27 (62%) Frame = +3 Query: 93 DSYGLGALVLAHSLRRAGSVYPAVALI 173 +S G L L H+LR+ G+++P A + Sbjct: 312 NSTEFGWLALTHALRKKGAIFPIHAYL 338 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,011,884 Number of Sequences: 5004 Number of extensions: 37292 Number of successful extensions: 109 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 104 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 108 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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