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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30288
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   254   6e-69
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...   136   2e-33
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...   126   3e-30
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...    96   3e-21
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...    76   4e-15
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...    76   4e-15
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...    64   9e-12
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr...    31   0.10 
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po...    30   0.18 
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    28   0.72 
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce...    26   3.8  
SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch...    25   6.7  
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo...    25   6.7  
SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    25   8.9  
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch...    25   8.9  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  254 bits (622), Expect = 6e-69
 Identities = 111/123 (90%), Positives = 120/123 (97%)
 Frame = +3

Query: 51  DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 230
           ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2   EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61

Query: 231 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 410
           RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ
Sbjct: 62  RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121

Query: 411 IMF 419
           I+F
Sbjct: 122 IIF 124



 Score = 48.0 bits (109), Expect = 8e-07
 Identities = 22/29 (75%), Positives = 24/29 (82%)
 Frame = +2

Query: 416 VXETXNSPAMYVXIQAVLSLYXSGRTTGI 502
           + ET N+PA YV IQAVLSLY SGRTTGI
Sbjct: 123 IFETFNAPAFYVAIQAVLSLYASGRTTGI 151


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score =  136 bits (329), Expect = 2e-33
 Identities = 62/122 (50%), Positives = 84/122 (68%), Gaps = 1/122 (0%)
 Frame = +3

Query: 57  DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 236
           D   + +DNGSG  KAGFAGDD P+ +FP+ VGR +H+ VM    QKD +VG EAQ+ RG
Sbjct: 8   DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67

Query: 237 ILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREKMTQI 413
           +L ++ PIE GII NW DME+IW + + + +L   PEEHP+LLTE PL    N+EK+ + 
Sbjct: 68  LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127

Query: 414 MF 419
            +
Sbjct: 128 FY 129



 Score = 34.7 bits (76), Expect = 0.008
 Identities = 16/27 (59%), Positives = 19/27 (70%)
 Frame = +2

Query: 422 ETXNSPAMYVXIQAVLSLYXSGRTTGI 502
           ET N PA+   +Q VL+LY S RTTGI
Sbjct: 130 ETLNVPALSFSLQPVLALYASARTTGI 156


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score =  126 bits (303), Expect = 3e-30
 Identities = 58/121 (47%), Positives = 85/121 (70%), Gaps = 2/121 (1%)
 Frame = +3

Query: 63  AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 236
           A +V+DNG+G  K G+A D+ PR  FPSIVGRP  R +     +  KD  VGDEA++ R 
Sbjct: 4   APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63

Query: 237 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 416
           +L +KYP+E+GII ++++M ++W +TF+ +L++ P    +LLTE P+NP ANREKM + M
Sbjct: 64  LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123

Query: 417 F 419
           F
Sbjct: 124 F 124



 Score = 28.7 bits (61), Expect = 0.55
 Identities = 12/20 (60%), Positives = 16/20 (80%)
 Frame = +2

Query: 443 MYVXIQAVLSLYXSGRTTGI 502
           +YV IQAVLSLY  G ++G+
Sbjct: 132 VYVAIQAVLSLYAQGLSSGV 151


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score = 95.9 bits (228), Expect = 3e-21
 Identities = 55/141 (39%), Positives = 74/141 (52%), Gaps = 24/141 (17%)
 Frame = +3

Query: 69  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 197
           +++DNG+G  K G+AG+DAP  VFP+++   R  G   G         M  K        
Sbjct: 8   IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66

Query: 198 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 356
                 D ++G++A  K     +L YPI HG I NWD ME+ W  + +  LR  PE+H  
Sbjct: 67  RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126

Query: 357 LLTEAPLNPKANREKMTQIMF 419
           LLTE PLNP  NRE   +IMF
Sbjct: 127 LLTEPPLNPPENRENTAEIMF 147


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score = 75.8 bits (178), Expect = 4e-15
 Identities = 42/128 (32%), Positives = 74/128 (57%), Gaps = 1/128 (0%)
 Frame = +3

Query: 39  FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 218
           F+   +D+  LV+DNGS   +AG+ G+  P+ VF ++V R R +     + +  + VG++
Sbjct: 18  FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDR----KLSRTSTLVGND 72

Query: 219 AQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 395
              + G  ++ + P E  +I+NWD ME++  +TF  +L +   EHP+ +TE   NP   R
Sbjct: 73  TLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVR 131

Query: 396 EKMTQIMF 419
             MT+++F
Sbjct: 132 STMTELLF 139


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 75.8 bits (178), Expect = 4e-15
 Identities = 42/122 (34%), Positives = 65/122 (53%)
 Frame = +3

Query: 54  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 233
           D+V+A+V+D GS   + GF+G+D P+ V PS  G       + G    + Y+    +S  
Sbjct: 9   DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61

Query: 234 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 413
           G + +K  I +G + NWD    +W +    +L+  P EHP+L+TE   NP  NR K  + 
Sbjct: 62  G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120

Query: 414 MF 419
           MF
Sbjct: 121 MF 122


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score = 64.5 bits (150), Expect = 9e-12
 Identities = 35/124 (28%), Positives = 67/124 (54%), Gaps = 2/124 (1%)
 Frame = +3

Query: 54  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 233
           +++ +LV+D GS   + G+AG+++P  + PS      + GV   +  ++ YV DE Q   
Sbjct: 8   EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61

Query: 234 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 407
            I  + +K    +GII +W+     W      +L+V P E+ +++TE   NP++ R+++ 
Sbjct: 62  PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121

Query: 408 QIMF 419
           +  F
Sbjct: 122 EAAF 125


>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1517

 Score = 31.1 bits (67), Expect = 0.10
 Identities = 16/48 (33%), Positives = 26/48 (54%)
 Frame = +3

Query: 114 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 257
           G+  PRA F  ++  P H G+++ M  KD   G+E  S +G  + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547


>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 401

 Score = 30.3 bits (65), Expect = 0.18
 Identities = 28/121 (23%), Positives = 52/121 (42%), Gaps = 4/121 (3%)
 Frame = +3

Query: 69  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 248
           +V+DNG+   KAGFAG        P+ + R +        G +  ++G+E  +     TL
Sbjct: 8   IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57

Query: 249 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 416
           ++   H  G + +W     +W     N   + P   ++ +LLT+      +      Q++
Sbjct: 58  QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117

Query: 417 F 419
           F
Sbjct: 118 F 118


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 28.3 bits (60), Expect = 0.72
 Identities = 17/59 (28%), Positives = 27/59 (45%), Gaps = 4/59 (6%)
 Frame = +3

Query: 255 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMF 419
           PI+ G + +W+ ++  W H  Y+ L   P +    +PV L         +RE  TQ  F
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFF 170


>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1666

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 12/35 (34%), Positives = 21/35 (60%), Gaps = 2/35 (5%)
 Frame = +3

Query: 315  FYNELRVAPEEHPVLLTE--APLNPKANREKMTQI 413
            +Y  L    E+HP+LLT+  A L P+ +  ++ +I
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRI 1433


>SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter
           family|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 791

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
 Frame = -3

Query: 310 WCQIFS-MSSQLVMIPCSMGYFRVRIPLLL-WASSPT*ESFWPIPTITPWW 164
           WC+  S + SQ+  +   +G F    PL L WA   +  +    P ITPWW
Sbjct: 304 WCKPSSAVLSQIFGVKTGLGLF----PLTLDWAQISSLSN----PLITPWW 346


>SPBC1683.09c |frp1||ferric-chelate reductase
           Frp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 564

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 11/29 (37%), Positives = 17/29 (58%)
 Frame = +3

Query: 174 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 260
           +M+G     +Y+G  AQ KR +LT +  I
Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193


>SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 485

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 5/56 (8%)
 Frame = +3

Query: 81  NGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMG-----QKDSYVGDEAQSKR 233
           N +GM         + R  F S VG  +     VG       Q+DSY+ D++ S+R
Sbjct: 318 NATGMYGVSKGHSSSTRRPFFSDVGSSQPAEEFVGSSSSHGRQQDSYIADDSDSER 373


>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
           Y|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1002

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 14/35 (40%), Positives = 16/35 (45%)
 Frame = -2

Query: 278 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHH 174
           GDD+VLD V   +D    L L        AH D H
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFH 710


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,923,713
Number of Sequences: 5004
Number of extensions: 37017
Number of successful extensions: 131
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 125
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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