BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30281 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 340 7e-95 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 190 1e-49 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 187 1e-48 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 146 1e-36 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 91 7e-20 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 86 3e-18 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 77 1e-15 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 64 9e-12 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 47 1e-06 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 37 0.002 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.18 SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 2.2 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 2.9 SPAC18B11.06 |||U3 snoRNP-associated protein Lcp5 |Schizosacchar... 25 6.7 SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 6.7 SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 25 8.9 SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|... 25 8.9 SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe... 25 8.9 SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 25 8.9 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 340 bits (836), Expect = 7e-95 Identities = 156/170 (91%), Positives = 165/170 (97%) Frame = +3 Query: 6 GVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHSIITNWDDMEKIWHHTFYNELRVAPEEH 185 G+MVGMGQKDSYVGDEAQSKRGILTLKYPIEH I+ NWDDMEKIWHHTFYNELRVAPEEH Sbjct: 42 GIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEH 101 Query: 186 PVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 365 P LLTEAPLNPK+NREKMTQI+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+H Sbjct: 102 PCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTH 161 Query: 366 TVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRD 515 TVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F+TTAEREIVRD Sbjct: 162 TVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRD 211 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 190 bits (463), Expect = 1e-49 Identities = 84/162 (51%), Positives = 120/162 (74%) Frame = +3 Query: 30 KDSYVGDEAQSKRGILTLKYPIEHSIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 209 KD VGDEA++ R +L +KYP+E+ II ++++M ++W +TF+ +L++ P +LLTE P Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109 Query: 210 LNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGY 389 +NP ANREKM + MFE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESV 169 Query: 390 ALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRD 515 L H + RLD+AGRD T YL+ +L +GY+F TA+ E VR+ Sbjct: 170 VLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVRE 211 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 187 bits (455), Expect = 1e-48 Identities = 86/170 (50%), Positives = 123/170 (72%), Gaps = 1/170 (0%) Frame = +3 Query: 9 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHSIITNWDDMEKIWHHTFYNE-LRVAPEEH 185 VM QKD +VG EAQ+ RG+L ++ PIE II NW DME+IW + + ++ L PEEH Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106 Query: 186 PVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 365 P+LLTE PL N+EK+ + +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTH 166 Query: 366 TVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRD 515 +VPIY+G+++P AI + ++ GRD+TDYL L + G+ ++AE+EIVR+ Sbjct: 167 SVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVRE 216 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 146 bits (355), Expect = 1e-36 Identities = 75/167 (44%), Positives = 103/167 (61%), Gaps = 11/167 (6%) Frame = +3 Query: 33 DSYVGDEAQSKRGI-LTLKYPIEHSIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 209 D ++G++A K +L YPI H I NWD ME+ W + + LR PE+H LLTE P Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132 Query: 210 LNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSH 365 LNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DSGDGV+H Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTH 192 Query: 366 TVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAER 500 +P+ EGY + +I + LAGRD+T ++ +L +R S TAER Sbjct: 193 IIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER 239 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 91.5 bits (217), Expect = 7e-20 Identities = 47/157 (29%), Positives = 88/157 (56%), Gaps = 3/157 (1%) Frame = +3 Query: 21 MGQKDSYVGDEAQSKRGILTL-KYPIEHSIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 197 + + + VG++ + G ++ + P E ++I+NWD ME++ +TF +L + EHP+ + Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120 Query: 198 TEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTV 371 TE NP R MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ SH + Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVI 180 Query: 372 PIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 482 P+ G + R+ G + YL+K+ + SF Sbjct: 181 PVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSF 217 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 86.2 bits (204), Expect = 3e-18 Identities = 42/134 (31%), Positives = 68/134 (50%) Frame = +3 Query: 75 LTLKYPIEHSIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 254 + +K I + + NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 255 ETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRD 434 E+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L +G Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSGNA 182 Query: 435 LTDYLMKILTERGY 476 + D L + L ++ + Sbjct: 183 INDILAQTLRDKNF 196 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 77.4 bits (182), Expect = 1e-15 Identities = 42/161 (26%), Positives = 79/161 (49%), Gaps = 2/161 (1%) Frame = +3 Query: 6 GVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHSIITNWDDMEKIWHHTFYNELRVAPE 179 GV + ++ YV DE Q I + +K + II +W+ W +L+V P Sbjct: 41 GVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPT 100 Query: 180 EHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGV 359 E+ +++TE NP++ R+++ + FE + PA Y+ QAV +A+ ++T +++D G Sbjct: 101 EYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDN 160 Query: 360 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 482 + P+ +G + I + LAG L + ++ F Sbjct: 161 ASVTPVVDGLIIRKGIFKQSLAGDFLNANIEQLFNTMNIEF 201 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 64.5 bits (150), Expect = 9e-12 Identities = 39/130 (30%), Positives = 62/130 (47%), Gaps = 4/130 (3%) Frame = +3 Query: 90 PIEHSIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 257 PI+ + +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 258 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDL 437 P +A + ++ LYA G G+V+D G + PI +G + A +L L GR + Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231 Query: 438 TDYLMKILTE 467 T +L +L E Sbjct: 232 TQHLQNLLRE 241 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 47.2 bits (107), Expect = 1e-06 Identities = 37/165 (22%), Positives = 71/165 (43%), Gaps = 18/165 (10%) Frame = +3 Query: 39 YVGDEAQSKRGILTLKYPIEHS--IITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEA 206 ++G+E + TL++ H + +W +W N + P ++ +LLT+ Sbjct: 43 FLGNELANCNDFTTLQFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQP 102 Query: 207 PLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTG--------------IVLD 344 + Q++FE F A A L + G T +V+D Sbjct: 103 VFTMPSIEHNTIQLVFEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVID 162 Query: 345 SGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYS 479 SG +H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ Sbjct: 163 SGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYN 207 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 36.7 bits (81), Expect = 0.002 Identities = 19/101 (18%), Positives = 48/101 (47%) Frame = +3 Query: 180 EHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGV 359 ++ V+ L + EK+ I+F + + +++ + + +G + V+D G Sbjct: 243 QYSVIFIVPDLYDRVYVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQK 302 Query: 360 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 482 + + EG +P++ ++++ G D+T MK+L + + Sbjct: 303 TSISCVEEGVVVPNSRIKINYGGDDITLLFMKLLMRSHFPY 343 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 30.3 bits (65), Expect = 0.18 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +3 Query: 150 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 293 +Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 26.6 bits (56), Expect = 2.2 Identities = 9/16 (56%), Positives = 9/16 (56%) Frame = +1 Query: 376 STKVTLCPTPSSVWTW 423 STK CP PS W W Sbjct: 52 STKTATCPDPSWEWAW 67 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 2.9 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +3 Query: 192 LLTEAPLNPKANREKMTQIMFETFN 266 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPAC18B11.06 |||U3 snoRNP-associated protein Lcp5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 327 Score = 25.0 bits (52), Expect = 6.7 Identities = 10/27 (37%), Positives = 15/27 (55%) Frame = +2 Query: 377 LRRLRSAPRHPPSGLGWSRLDRLPHED 457 L++ + +H G WS LDR H+D Sbjct: 254 LKKSKRVKKHDYGGEDWSLLDRKFHDD 280 >SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 564 Score = 25.0 bits (52), Expect = 6.7 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +3 Query: 9 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 95 +M+G +Y+G AQ KR +LT + I Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 24.6 bits (51), Expect = 8.9 Identities = 14/35 (40%), Positives = 16/35 (45%) Frame = -2 Query: 113 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHH 9 GDD+VLD V +D L L AH D H Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFH 710 >SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 341 Score = 24.6 bits (51), Expect = 8.9 Identities = 13/46 (28%), Positives = 28/46 (60%), Gaps = 3/46 (6%) Frame = +3 Query: 90 PIEHSIITNWDDME-KIWH--HTFYNELRVAPEEHPVLLTEAPLNP 218 P + +I + DD +W+ HTF+++ + P EH + ++++ +NP Sbjct: 266 PQGNFVIGSADDRTLNVWNLRHTFHHKGKTRPPEHRI-VSQSIINP 310 >SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe|chr 3|||Manual Length = 301 Score = 24.6 bits (51), Expect = 8.9 Identities = 10/16 (62%), Positives = 11/16 (68%), Gaps = 1/16 (6%) Frame = +1 Query: 361 PTPYPSTKVTLC-PTP 405 P P P TKV +C PTP Sbjct: 256 PAPSPETKVLICGPTP 271 >SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces pombe|chr 1|||Manual Length = 721 Score = 24.6 bits (51), Expect = 8.9 Identities = 7/18 (38%), Positives = 13/18 (72%) Frame = +3 Query: 345 SGDGVSHTVPIYEGYALP 398 +G G+ H + + +GYA+P Sbjct: 157 AGRGIGHQIMVEQGYAMP 174 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,960,413 Number of Sequences: 5004 Number of extensions: 36996 Number of successful extensions: 144 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 134 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 140 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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