BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30258 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 105 3e-24 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 60 2e-10 SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 59 3e-10 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 56 4e-09 SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 41 1e-04 SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 33 0.025 SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 31 0.10 SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p... 29 0.31 SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 27 1.7 SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 26 3.8 SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 26 3.8 SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 25 5.1 SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc... 25 6.7 SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual 25 6.7 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 105 bits (253), Expect = 3e-24 Identities = 52/148 (35%), Positives = 88/148 (59%) Frame = +3 Query: 57 LPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRL 236 L EQIA R+AF FD ++ G+I + + ++R +GQ L+++I EVDAD +G + Sbjct: 6 LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTI 65 Query: 237 EFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXX 416 +F EF+T+ A+ + + D E +E+REAF+++DK+GNGYI L + Sbjct: 66 DFTEFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEE 122 Query: 417 XXXXIQEIDTDGSGTVDFDEFMEMMTGE 500 I+E DTDG G ++++EF +++ + Sbjct: 123 VADMIREADTDGDGVINYEEFSRVISSK 150 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 60.1 bits (139), Expect = 2e-10 Identities = 38/149 (25%), Positives = 68/149 (45%) Frame = +3 Query: 54 ELPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGR 233 E+ EQ + +AF FD ++ +I + +R +G K + +++ + D G Sbjct: 30 EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89 Query: 234 LEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXX 413 L+ +FV + + IVE D +E++ AF L+D + G I +LR + Sbjct: 90 LQMEDFVRVMTEKIVERDP---LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQ 146 Query: 414 XXXXXIQEIDTDGSGTVDFDEFMEMMTGE 500 I+E D D G ++ EF+ +M E Sbjct: 147 ELEAMIEEFDLDQDGEINEQEFIAIMMDE 175 Score = 39.1 bits (87), Expect = 4e-04 Identities = 21/75 (28%), Positives = 40/75 (53%) Frame = +3 Query: 36 TEDWADELPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVD 215 TE + P E+I ++AF+ FD + +G I + + + + + + + LE +IEE D Sbjct: 100 TEKIVERDPLEEI---KRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFD 156 Query: 216 ADKSGRLEFPEFVTL 260 D+ G + EF+ + Sbjct: 157 LDQDGEINEQEFIAI 171 >SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 174 Score = 59.3 bits (137), Expect = 3e-10 Identities = 42/149 (28%), Positives = 68/149 (45%), Gaps = 5/149 (3%) Frame = +3 Query: 66 EQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFP 245 E+I +RK F D N+SGSI + I + P + L VD D G ++F Sbjct: 21 EEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNP----LASRLFSVVDEDGGGDVDFQ 76 Query: 246 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSL-----REIXXXXXXXXXX 410 EF+ + F V + E ++L+ AF++YD + +GYI L + Sbjct: 77 EFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQ 133 Query: 411 XXXXXXIQEIDTDGSGTVDFDEFMEMMTG 497 I E+D D G + F+EF ++++G Sbjct: 134 QIVDKTIMEVDKDRDGKISFEEFKDIVSG 162 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 55.6 bits (128), Expect = 4e-09 Identities = 35/140 (25%), Positives = 68/140 (48%) Frame = +3 Query: 66 EQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFP 245 EQ +++AF +D ++ G IP V +LR +G L +L E+ ++ Sbjct: 6 EQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GDAIDEK 61 Query: 246 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXX 425 +F++ + + E ++E +E +AFR++DK+ +GYI T+ + Sbjct: 62 KFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118 Query: 426 XIQEIDTDGSGTVDFDEFME 485 +QE D SG+ D+ +F++ Sbjct: 119 MVQEADPTNSGSFDYYDFVQ 138 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 40.7 bits (91), Expect = 1e-04 Identities = 30/136 (22%), Positives = 62/136 (45%) Frame = +3 Query: 84 RKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEFVTLA 263 ++AF FD + +G IP + D+LR GQ N + E I E+++ ++ +F+ + Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDLLRACGQ--NPTLAE--ITEIESTLPAEVDMEQFLQVL 64 Query: 264 AKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXXIQEID 443 + D +E + F+++DK+ G I LR + ++ + Sbjct: 65 NR-PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVP 123 Query: 444 TDGSGTVDFDEFMEMM 491 G V++ +F++M+ Sbjct: 124 VK-DGMVNYHDFVQMI 138 >SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 190 Score = 33.1 bits (72), Expect = 0.025 Identities = 21/63 (33%), Positives = 32/63 (50%) Frame = +3 Query: 192 EELIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSL 371 E + DADK+G ++F EF+ + V E + +L AF+LYD + NG I + Sbjct: 66 EYVFNVFDADKNGYIDFKEFICALS---VTSRGE-LNDKLIWAFQLYDLDNNGLISYDEM 121 Query: 372 REI 380 I Sbjct: 122 LRI 124 >SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 899 Score = 31.1 bits (67), Expect = 0.10 Identities = 15/53 (28%), Positives = 28/53 (52%) Frame = +3 Query: 93 FDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEF 251 ++ + +S + V + +M+ + + LEE ++ DAD SG+L F EF Sbjct: 303 WEKLEKEQSAQLDLGDVHRMCQMLHLNASMEFLEETFQKADADHSGKLSFEEF 355 >SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces pombe|chr 1|||Manual Length = 2280 Score = 29.5 bits (63), Expect = 0.31 Identities = 19/65 (29%), Positives = 31/65 (47%), Gaps = 1/65 (1%) Frame = -2 Query: 479 ELVEVDGAASVSV-DLLDEAIEFIVSQLLVQFPEDLAQARRGNVTVAFLVVQSERFPEFF 303 EL V A S DL D AI ++Q+L F +DL + VT+ + +P ++ Sbjct: 1246 ELTNVVNVALTSTGDLDDSAIVSKLNQILSDFRDDLLEHNVRRVTIVGGRINKSAYPSYY 1305 Query: 302 LHGLS 288 + +S Sbjct: 1306 TYRVS 1310 >SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 27.1 bits (57), Expect = 1.7 Identities = 11/18 (61%), Positives = 15/18 (83%) Frame = +3 Query: 303 KELREAFRLYDKEGNGYI 356 +EL+EAF L DK+G+G I Sbjct: 48 QELKEAFALLDKDGDGNI 65 >SPAC1687.14c |||EF hand family protein, unknown role|Schizosaccharomyces pombe|chr 1|||Manual Length = 76 Score = 25.8 bits (54), Expect = 3.8 Identities = 12/26 (46%), Positives = 14/26 (53%) Frame = +3 Query: 297 MQKELREAFRLYDKEGNGYIPTSSLR 374 M +E EAF L+D GYI LR Sbjct: 10 MDEEAEEAFDLFDVTHKGYIDFEDLR 35 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 25.8 bits (54), Expect = 3.8 Identities = 15/36 (41%), Positives = 21/36 (58%) Frame = +1 Query: 373 ARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSS 480 A ++G+ TSS T + SS S T +A +STSS Sbjct: 968 ASATGSSTSSIATASVSGSSTSSVATASATDSSTSS 1003 Score = 25.4 bits (53), Expect = 5.1 Identities = 16/38 (42%), Positives = 21/38 (55%) Frame = +1 Query: 367 ACARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSS 480 A A +G+ TSS T + SS S T +A +STSS Sbjct: 1005 AAASVTGSSTSSVATASVTDSSTSSVATASATDSSTSS 1042 >SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Manual Length = 614 Score = 25.4 bits (53), Expect = 5.1 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = +3 Query: 189 LEELIEEVDADKSGRLEFPEFV 254 + E I EV+ D SGR+E +FV Sbjct: 56 VREAIREVNVDSSGRVEPEDFV 77 >SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 830 Score = 25.0 bits (52), Expect = 6.7 Identities = 15/34 (44%), Positives = 20/34 (58%) Frame = +3 Query: 243 PEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEG 344 PEF AA+ +VEED E + EA + D+EG Sbjct: 38 PEFSDEAAEALVEEDIEDLD---GEALDVEDEEG 68 >SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual Length = 341 Score = 25.0 bits (52), Expect = 6.7 Identities = 12/40 (30%), Positives = 17/40 (42%) Frame = +1 Query: 364 RACARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSSW 483 R C S +W SS +T + S+R L S + W Sbjct: 59 RLCKLPSKSWVSSTVTNEKLRESRRLALQAYVQCLSETPW 98 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,751,608 Number of Sequences: 5004 Number of extensions: 29930 Number of successful extensions: 165 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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