Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30238
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondri... 128 2e-30
At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast... 124 3e-29
At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast... 124 3e-29
At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast... 119 1e-27
At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic... 118 3e-27
At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic... 99 2e-21
At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic... 96 1e-20
At4g37235.1 68417.m05271 expressed protein 30 0.80
At4g39840.1 68417.m05645 expressed protein 28 4.3
At1g79820.2 68414.m09323 hexose transporter, putative similar to... 28 4.3
At1g79820.1 68414.m09322 hexose transporter, putative similar to... 28 4.3
At3g54460.1 68416.m06025 SNF2 domain-containing protein / helica... 27 9.9
At1g48640.1 68414.m05444 lysine and histidine specific transport... 27 9.9
>At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondrial
/ transaminase A (ASP1) identical to SP|P46643 Aspartate
aminotransferase, mitochondrial precursor (EC 2.6.1.1)
(Transaminase A) {Arabidopsis thaliana}
Length = 430
Score = 128 bits (310), Expect = 2e-30
Identities = 64/120 (53%), Positives = 81/120 (67%)
Frame = +2
Query: 128 GLRASSTWWNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAE 307
GLR+ S+WW +V+ P D ILG+TEA+ D P+KVN+GVGAYRDD GKP VL VR+AE
Sbjct: 25 GLRSMSSWWKSVEPAPKDPILGVTEAFLADPSPEKVNVGVGAYRDDNGKPVVLECVREAE 84
Query: 308 EILHSRGLNHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEF 487
+ L + EY P+ G A D KLA+G++S IK+K VQTLSGTGA RL +F
Sbjct: 85 KRL-AGSTFMEYLPMGGSAKMVDLTLKLAYGDNSEFIKDKRIAAVQTLSGTGACRLFADF 143
>At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast /
transaminase A (ASP5) (AAT1) nearly identical to
SP|P46248 Aspartate aminotransferase, chloroplast
precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
thaliana}
Length = 453
Score = 124 bits (300), Expect = 3e-29
Identities = 60/121 (49%), Positives = 81/121 (66%)
Frame = +2
Query: 152 WNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGL 331
+ + M PPD ILG++EA+K DT+ K+NLGVGAYR +E +P+VL V+KAE ++ RG
Sbjct: 53 FEGITMAPPDPILGVSEAFKADTNGMKLNLGVGAYRTEELQPYVLNVVKKAENLMLERGD 112
Query: 332 NHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAKA 511
N EY PI G A + A A+L FG PVIK + T+Q LSGTG+LRL I +++ A
Sbjct: 113 NKEYLPIEGLAAFNKATAELLFGAGHPVIKEQRVATIQGLSGTGSLRLAAALIERYFPGA 172
Query: 512 K 514
K
Sbjct: 173 K 173
>At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast /
transaminase A (ASP5) (AAT1) nearly identical to
SP|P46248 Aspartate aminotransferase, chloroplast
precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
thaliana}
Length = 453
Score = 124 bits (300), Expect = 3e-29
Identities = 60/121 (49%), Positives = 81/121 (66%)
Frame = +2
Query: 152 WNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGL 331
+ + M PPD ILG++EA+K DT+ K+NLGVGAYR +E +P+VL V+KAE ++ RG
Sbjct: 53 FEGITMAPPDPILGVSEAFKADTNGMKLNLGVGAYRTEELQPYVLNVVKKAENLMLERGD 112
Query: 332 NHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAKA 511
N EY PI G A + A A+L FG PVIK + T+Q LSGTG+LRL I +++ A
Sbjct: 113 NKEYLPIEGLAAFNKATAELLFGAGHPVIKEQRVATIQGLSGTGSLRLAAALIERYFPGA 172
Query: 512 K 514
K
Sbjct: 173 K 173
>At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast /
transaminase A (ASP3) (YLS4) identical to SP|P46644
Aspartate aminotransferase, chloroplast precursor (EC
2.6.1.1) (Transaminase A) {Arabidopsis thaliana};
identical to cDNA YLS4 mRNA for aspartate
aminotransferase (ASP3), partial cds GI:13122285
Length = 449
Score = 119 bits (286), Expect = 1e-27
Identities = 60/118 (50%), Positives = 77/118 (65%), Gaps = 1/118 (0%)
Frame = +2
Query: 152 WNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAE-EILHSRG 328
++++ P D ILG+T AY KD P K+NLGVGAYR +EGKP VL VRKAE ++++ R
Sbjct: 49 FSHLVQAPEDPILGVTVAYNKDPSPVKLNLGVGAYRTEEGKPLVLNVVRKAEQQLINDRT 108
Query: 329 LNHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHY 502
EY PI G + AKL G DSP I+ TV+ LSGTG+LR+G EF+ KHY
Sbjct: 109 RIKEYLPIVGLVEFNKLSAKLILGADSPAIRENRITTVECLSGTGSLRVGGEFLAKHY 166
>At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic
isozyme 1 / transaminase A (ASP2) identical to SP|P46645
Aspartate aminotransferase, cytoplasmic isozyme 1 (EC
2.6.1.1) (Transaminase A) {Arabidopsis thaliana}
Length = 405
Score = 118 bits (283), Expect = 3e-27
Identities = 60/118 (50%), Positives = 74/118 (62%), Gaps = 1/118 (0%)
Frame = +2
Query: 152 WNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAE-EILHSRG 328
++NV P D ILG+T AY D P K+NLGVGAYR +EGKP VL VRKAE ++++
Sbjct: 5 FSNVARAPEDPILGVTVAYNNDPSPVKINLGVGAYRTEEGKPLVLDVVRKAEQQLVNDPS 64
Query: 329 LNHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHY 502
EY PI G + + AKL G DSP I TVQ LSGTG+LR+G EF+ HY
Sbjct: 65 RVKEYIPIVGISDFNKLSAKLILGADSPAITESRVTTVQCLSGTGSLRVGAEFLKTHY 122
>At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic
isozyme 2 / transaminase A (ASP4) identical to aspartate
aminotransferase, cytoplasmic isozyme 2 SP:P46646
[Arabidopsis thaliana]
Length = 403
Score = 98.7 bits (235), Expect = 2e-21
Identities = 49/115 (42%), Positives = 68/115 (59%)
Frame = +2
Query: 155 NNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGLN 334
++V P D +L + A + D P K+NL G YR +EGKP VL VR+AE+ L + L+
Sbjct: 6 SSVLPAPEDPVLSVIFACRDDPSPVKLNLSAGTYRTEEGKPLVLDVVRRAEQQL-ANDLD 64
Query: 335 HEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKH 499
EY P++G + KL G+DSP +K T Q LSGTG+LR+G EF+ H
Sbjct: 65 KEYLPLNGLPEFNKLSTKLILGDDSPALKENRVVTTQCLSGTGSLRVGAEFLATH 119
>At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic
isozyme 2 / transaminase A (ASP4) identical to aspartate
aminotransferase, cytoplasmic isozyme 2 SP:P46646
[Arabidopsis thaliana]
Length = 405
Score = 95.9 bits (228), Expect = 1e-20
Identities = 48/116 (41%), Positives = 67/116 (57%), Gaps = 1/116 (0%)
Frame = +2
Query: 155 NNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEIL-HSRGL 331
++V P D +L + A + D P K+NL G YR +EGKP VL VR+AE+ L +
Sbjct: 6 SSVLPAPEDPVLSVIFACRDDPSPVKLNLSAGTYRTEEGKPLVLDVVRRAEQQLANDLSR 65
Query: 332 NHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKH 499
+ EY P++G + KL G+DSP +K T Q LSGTG+LR+G EF+ H
Sbjct: 66 DKEYLPLNGLPEFNKLSTKLILGDDSPALKENRVVTTQCLSGTGSLRVGAEFLATH 121
>At4g37235.1 68417.m05271 expressed protein
Length = 152
Score = 30.3 bits (65), Expect = 0.80
Identities = 14/44 (31%), Positives = 26/44 (59%), Gaps = 4/44 (9%)
Frame = -3
Query: 454 GQCLYSTVALIF----DDRAVFSKSQFSYSISICGLTTDGSILM 335
GQ L+S+ +L+F DD ++ + F Y +++ GL T S+ +
Sbjct: 19 GQTLFSSASLLFMCFNDDEDFYAYTTFCYLVTVMGLVTPWSVTL 62
>At4g39840.1 68417.m05645 expressed protein
Length = 451
Score = 27.9 bits (59), Expect = 4.3
Identities = 24/94 (25%), Positives = 36/94 (38%)
Frame = +2
Query: 50 KMAQVLKKLTTQVLKPNNVDTIVGCTGLRASSTWWNNVQMGPPDVILGITEAYKKDTHPK 229
K + LKKL + N+ +I L SS+ N + PP L + KK + P
Sbjct: 136 KTSSELKKLNSGTKSTNSTSSIKKSADLSKSSSSKNKTTIKPPSSKLSSPPSEKK-SQPS 194
Query: 230 KVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGL 331
+ + E KPF L + + R L
Sbjct: 195 SKPVTKSKQSEKEIKPFWLDDEEDEDFVSEFRDL 228
>At1g79820.2 68414.m09323 hexose transporter, putative similar to
hexose transporter GI:8347246 from (Solanum tuberosum);
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 495
Score = 27.9 bits (59), Expect = 4.3
Identities = 20/81 (24%), Positives = 35/81 (43%)
Frame = -1
Query: 249 PTPRFTFLGCVSFLYASVMPKITSGGPI*TLFHQVLLALRPVQPTIVSTLLGLSTWVVNF 70
P+P T V + V+ T GP+ +L + R ++ T ++ L + WV+NF
Sbjct: 381 PSPFGTLFLSVGGMLLFVLSFATGAGPVPSLLLSEICPGR-LRATALAVCLAVH-WVINF 438
Query: 69 FNT*AIFTKLSNTGKIITNYV 7
F L G ++ N +
Sbjct: 439 FVGLLFLRMLEQLGSVLLNAI 459
>At1g79820.1 68414.m09322 hexose transporter, putative similar to
hexose transporter GI:8347246 from (Solanum tuberosum);
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 495
Score = 27.9 bits (59), Expect = 4.3
Identities = 20/81 (24%), Positives = 35/81 (43%)
Frame = -1
Query: 249 PTPRFTFLGCVSFLYASVMPKITSGGPI*TLFHQVLLALRPVQPTIVSTLLGLSTWVVNF 70
P+P T V + V+ T GP+ +L + R ++ T ++ L + WV+NF
Sbjct: 381 PSPFGTLFLSVGGMLLFVLSFATGAGPVPSLLLSEICPGR-LRATALAVCLAVH-WVINF 438
Query: 69 FNT*AIFTKLSNTGKIITNYV 7
F L G ++ N +
Sbjct: 439 FVGLLFLRMLEQLGSVLLNAI 459
>At3g54460.1 68416.m06025 SNF2 domain-containing protein / helicase
domain-containing protein / F-box family protein similar
to SP|P36607 DNA repair protein rad8 {Schizosaccharomyces
pombe}; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain, PF00646: F-box domain
Length = 1378
Score = 26.6 bits (56), Expect = 9.9
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +2
Query: 413 VIKNKSNCTVQTLSGTGALRLGLEFIT 493
+ +N ++C + G+GAL L L F+T
Sbjct: 1238 MFQNDADCMALLMDGSGALGLDLSFVT 1264
>At1g48640.1 68414.m05444 lysine and histidine specific transporter,
putative similar to lysine and histidine specific
transporter GI:2576361 from [Arabidopsis thaliana];
contains Pfam profile PF01490: Transmembrane amino acid
transporter protein
Length = 453
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/55 (27%), Positives = 32/55 (58%)
Frame = -1
Query: 420 LMTGLSSPKASLATASVYVASPLMGAYS*LSPLLWRISSAFLTEGKTNGFPSSSL 256
++ L +P ++ATA+++V ++G+Y + ++ + FL + K N PS+ L
Sbjct: 305 VLMSLETPVWAIATANLFVVMHVIGSYQIFAMPVFDMVETFLVK-KLNFKPSTVL 358
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,869,063
Number of Sequences: 28952
Number of extensions: 257350
Number of successful extensions: 645
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 628
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 640
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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