BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30235 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subun... 99 3e-22 SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharo... 27 1.3 SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 27 1.7 SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase Ppk38|Schizo... 27 2.2 SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyce... 27 2.2 SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomy... 27 2.2 SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ... 26 2.9 SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |S... 26 2.9 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 26 2.9 SPAC24H6.11c |||sulfate transporter |Schizosaccharomyces pombe|c... 26 2.9 SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 3.8 SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22... 26 3.8 SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 26 3.8 SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3 |Schizosacchar... 25 6.7 SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces... 25 6.7 SPBC1539.10 |||ribosome biogenesis protein Nop16 |Schizosaccharo... 25 8.9 SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces po... 25 8.9 >SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subunit Cgs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 412 Score = 99.1 bits (236), Expect = 3e-22 Identities = 66/168 (39%), Positives = 96/168 (57%), Gaps = 13/168 (7%) Frame = +2 Query: 47 RRKSVFAETYDPEEDDSDEGAPAVFPKSDAQRA-RLAEAVRGILLFRSLDAQQMQQVLDA 223 RR+SV E+ +P + FP D + RL +V G LF++LD + +VL+A Sbjct: 101 RRQSVSTESMNPSAFALE--TKRTFPPKDPEDLKRLKRSVAGNFLFKNLDEEHYNEVLNA 158 Query: 224 MFEKR-SEPGEYVIRQGDDGDNFYVIENGVFDVL------VTGDDRVE----KVVHTYEG 370 M EKR E G VI QG GD FY++E G FDV +T ++ + + T Sbjct: 159 MTEKRIGEAGVAVIVQGAVGDYFYIVEQGEFDVYKRPELNITPEEVLSSGYGNYITTISP 218 Query: 371 SGSFGELALMYNMPRAASVRAQTA-GALWAMDRHTFRRILLKSAFKKR 511 FGELALMYN PRAASV ++T ++A+DR +FRRI+ ++A+++R Sbjct: 219 GEYFGELALMYNAPRAASVVSKTPNNVIYALDRTSFRRIVFENAYRQR 266 Score = 73.3 bits (172), Expect = 2e-14 Identities = 46/124 (37%), Positives = 67/124 (54%) Frame = +2 Query: 116 VFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDAMFEKRSEPGEYVIRQGDDGDNFYV 295 VF + QR + + + SLD Q Q++ DA+ + G VIRQGD G+ FY+ Sbjct: 258 VFENAYRQRMLYESLLEEVPILSSLDKYQRQKIADALQTVVYQAGSIVIRQGDIGNQFYL 317 Query: 296 IENGVFDVLVTGDDRVEKVVHTYEGSGSFGELALMYNMPRAASVRAQTAGALWAMDRHTF 475 IE+G +V+ G + VV T FGELAL++ R A+V+A+T L D+ TF Sbjct: 318 IEDGEAEVVKNG----KGVVVTLTKGDYFGELALIHETVRNATVQAKTRLKLATFDKPTF 373 Query: 476 RRIL 487 R+L Sbjct: 374 NRLL 377 >SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1388 Score = 27.5 bits (58), Expect = 1.3 Identities = 17/63 (26%), Positives = 31/63 (49%) Frame = +2 Query: 65 AETYDPEEDDSDEGAPAVFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDAMFEKRSE 244 AE+ EE +SD+ + PK +++ + V+ + F S +++ + DA FE E Sbjct: 111 AESVTSEESESDDSSYGGTPKKRSRQKKSNTYVQDEIRFSSRNSKGVNYNEDAYFESFEE 170 Query: 245 PGE 253 E Sbjct: 171 EEE 173 >SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 317 Score = 27.1 bits (57), Expect = 1.7 Identities = 24/91 (26%), Positives = 41/91 (45%), Gaps = 3/91 (3%) Frame = -1 Query: 366 SYVWTTFSTRSSPV---TRTSKTPFSMT*KLSPSSPCLITYSPGSDLFSNIASRTCCICC 196 SY T ST +P TRTS + F + +PSS + S S S +S + Sbjct: 122 SYSGTISSTSIAPSMIGTRTSSSYFITSSSSTPSSSSSSSSSSPSSSSSKSSSSSKSSSS 181 Query: 195 ASRERNSSMPRTASARRALCASDLGNTAGAP 103 +S SS ++S++ + +S ++ +P Sbjct: 182 SSSSSKSSSSSSSSSKSSSSSSSSSKSSASP 212 >SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase Ppk38|Schizosaccharomyces pombe|chr 3|||Manual Length = 650 Score = 26.6 bits (56), Expect = 2.2 Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 2/46 (4%) Frame = -1 Query: 342 TRSSPVTRT--SKTPFSMT*KLSPSSPCLITYSPGSDLFSNIASRT 211 T+ +P + T P K+SP++P L T + D+FS+ A T Sbjct: 416 TQGAPPSHTYGPPPPVQPKPKISPTTPRLSTLALADDMFSSTAKET 461 >SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 26.6 bits (56), Expect = 2.2 Identities = 12/49 (24%), Positives = 25/49 (51%) Frame = +2 Query: 77 DPEEDDSDEGAPAVFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDA 223 D ++DD ++ PAV +S R+++ + RS ++Q+ D+ Sbjct: 426 DDDDDDPNDFRPAVERQSSTMNTRMSQPSSAVNTNRSYGSEQIPSYADS 474 >SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomyces pombe|chr 1|||Manual Length = 348 Score = 26.6 bits (56), Expect = 2.2 Identities = 11/40 (27%), Positives = 23/40 (57%) Frame = +2 Query: 341 VEKVVHTYEGSGSFGELALMYNMPRAASVRAQTAGALWAM 460 ++ +HTY+G S + L+ ++P ++ + TA L +M Sbjct: 267 IDSFIHTYDGFSSMTQCLLIISLPLSSLWASSTALKLQSM 306 >SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 932 Score = 26.2 bits (55), Expect = 2.9 Identities = 19/65 (29%), Positives = 32/65 (49%), Gaps = 3/65 (4%) Frame = -1 Query: 336 SSPVTRTSKTPFSMT*KLSPS---SPCLITYSPGSDLFSNIASRTCCICCASRERNSSMP 166 SSP+ R TP S ++PS P S G+ +++A+ + + + ERN SM Sbjct: 459 SSPIMRADSTPISHNLAVTPSFSPIPQQSNKSIGNHKNTSVANVSSKVANSKFERNISMM 518 Query: 165 RTASA 151 ++A Sbjct: 519 SNSNA 523 >SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1206 Score = 26.2 bits (55), Expect = 2.9 Identities = 16/55 (29%), Positives = 27/55 (49%), Gaps = 2/55 (3%) Frame = +2 Query: 296 IENGVFDVLVTGDDRVEKVVHTYEGSGSFGELALMY--NMPRAASVRAQTAGALW 454 ++ F +L TGD + K+ ++G G+ EL L Y +P A + G L+ Sbjct: 313 MKGSFFYLLQTGDGDLLKLTIEHDGQGNVVELRLKYFDTVPLAVQLNILKTGFLF 367 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 26.2 bits (55), Expect = 2.9 Identities = 20/80 (25%), Positives = 37/80 (46%), Gaps = 4/80 (5%) Frame = +2 Query: 110 PAVFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDAMFEKRSEPGEYVIRQGDDGDNF 289 PA F + Q + A + G+ + R ++ + + + +E ++++ G F Sbjct: 178 PAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDTE--KHIVVYDLGGGTF 235 Query: 290 YV----IENGVFDVLVTGDD 337 V I+NGVF+VL T D Sbjct: 236 DVSLLSIDNGVFEVLATSGD 255 >SPAC24H6.11c |||sulfate transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 958 Score = 26.2 bits (55), Expect = 2.9 Identities = 20/83 (24%), Positives = 33/83 (39%) Frame = +2 Query: 248 GEYVIRQGDDGDNFYVIENGVFDVLVTGDDRVEKVVHTYEGSGSFGELALMYNMPRAASV 427 G+ + R+GD ++E G+ D E + GEL A+V Sbjct: 844 GDLLWRKGDTPSKLVILETGMVKASYQMDR--ESLSENITCLCVVGELPFFSKTCYNATV 901 Query: 428 RAQTAGALWAMDRHTFRRILLKS 496 A+ A+W +DR + +L S Sbjct: 902 VAELDSAVWILDREGWETMLKSS 924 >SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 395 Score = 25.8 bits (54), Expect = 3.8 Identities = 21/68 (30%), Positives = 34/68 (50%), Gaps = 3/68 (4%) Frame = +2 Query: 137 QRARLAEAVRG--ILLFRSLDAQQMQQVLDAM-FEKRSEPGEYVIRQGDDGDNFYVIENG 307 ++ L EAV + + + Q+ + L+AM FE +PG + D NF VI+N Sbjct: 320 EKQLLEEAVERYKLAMVEFAECQRRLKELEAMHFE---QPGSVTEKLFPDPTNFEVIDNV 376 Query: 308 VFDVLVTG 331 D+ +TG Sbjct: 377 SIDLTLTG 384 >SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22 |Schizosaccharomyces pombe|chr 1|||Manual Length = 332 Score = 25.8 bits (54), Expect = 3.8 Identities = 19/63 (30%), Positives = 33/63 (52%), Gaps = 1/63 (1%) Frame = -3 Query: 334 VSGDQDVENAVLDDVEVVAVIALSDYVLAGLGSLFEHRIQNL-LHLLRVKRTEQQYAPDR 158 + D+D+ + V DDV+ V +I +A LG +Q+L L ++K+ E P+ Sbjct: 26 IDADEDLLDDVPDDVDCVELIQSRIQSMASLGLERFKNLQSLCLRQNQIKKIES--VPET 83 Query: 157 LSE 149 L+E Sbjct: 84 LTE 86 >SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 25.8 bits (54), Expect = 3.8 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = +3 Query: 81 PKRMILTKEPLPCSPSRTHREPVSLRRSG 167 P R +++PLP SPSRT + + SG Sbjct: 78 PVRSPSSRKPLPASPSRTRDHSLRVPVSG 106 >SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 569 Score = 25.0 bits (52), Expect = 6.7 Identities = 10/20 (50%), Positives = 13/20 (65%) Frame = -3 Query: 259 YVLAGLGSLFEHRIQNLLHL 200 +VL G+G +H I NL HL Sbjct: 450 FVLCGMGGRVDHAIGNLNHL 469 >SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces pombe|chr 3|||Manual Length = 693 Score = 25.0 bits (52), Expect = 6.7 Identities = 22/91 (24%), Positives = 39/91 (42%), Gaps = 2/91 (2%) Frame = -3 Query: 316 VENAVLDDVEVVAVIALSDYVLAGLGSLFEHRIQNLLHLLRVKRTEQQYAPDRLSETGSL 137 ++N + D E + +LAGL E R Q LHL + + +L+ G++ Sbjct: 311 IDNTLFLDKERATSLNDMRSMLAGLSDFQELRDQYSLHLTMAQECMNIFEKQQLNLIGAI 370 Query: 136 --CVRLGEHGRGSFVRIILFGVISLGENGFA 50 + G + G R +L ++ L + G A Sbjct: 371 EQDLSTGSNVEGKVPRSVLSELLPLLDEGNA 401 >SPBC1539.10 |||ribosome biogenesis protein Nop16 |Schizosaccharomyces pombe|chr 2|||Manual Length = 209 Score = 24.6 bits (51), Expect = 8.9 Identities = 11/36 (30%), Positives = 21/36 (58%) Frame = +2 Query: 191 DAQQMQQVLDAMFEKRSEPGEYVIRQGDDGDNFYVI 298 D + + LD + +K PG+ ++R+ DDG+ +I Sbjct: 79 DRELTSEELDEL-KKSLPPGQAIVRRDDDGNIIEII 113 >SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 24.6 bits (51), Expect = 8.9 Identities = 17/57 (29%), Positives = 27/57 (47%), Gaps = 3/57 (5%) Frame = -3 Query: 169 APDRLSETGSLCVRLGE---HGRGSFVRIILFGVISLGENGFAPIVKARHWRLFYKI 8 A + L ET L ++L + HGR + I+L+G G++ A V F+ I Sbjct: 137 AKEALKETVLLPIKLPQLFSHGRKPWSGILLYGPPGTGKSYLAKAVATEAGSTFFSI 193 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,097,289 Number of Sequences: 5004 Number of extensions: 43565 Number of successful extensions: 195 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 175 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 191 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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