Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30224
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 82 4e-17
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 81 1e-16
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 55 5e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 41 1e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 40 2e-04
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 27 1.7
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 25 6.7
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 82.2 bits (194), Expect = 4e-17
Identities = 45/122 (36%), Positives = 66/122 (54%), Gaps = 2/122 (1%)
Frame = +2
Query: 155 SRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELD 334
++ AKR S F+ + Q+ E KEAF L+D D DG IG+ D++ SL + ASE ++
Sbjct: 28 AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87
Query: 335 EMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLRHALMTW 508
M + PIN LT + + S +D ++ AF TFD + GKI +R AL +
Sbjct: 88 HMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSM 146
Query: 509 GD 514
GD
Sbjct: 147 GD 148
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 80.6 bits (190), Expect = 1e-16
Identities = 44/111 (39%), Positives = 66/111 (59%), Gaps = 6/111 (5%)
Frame = +2
Query: 200 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 367
+ +Q+AEF+EAF L D D+DG I N+L SLG+ + EL +M+ E +G I+
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 368 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGD 514
FT+ LT+ A +M +D ++ V AFK FD++G I E L H L + G+
Sbjct: 67 FTEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGE 116
Score = 37.1 bits (82), Expect = 0.002
Identities = 20/65 (30%), Positives = 36/65 (55%), Gaps = 4/65 (6%)
Frame = +2
Query: 218 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLT 385
E +EAF++ D D +G I +L SLG S++E+ +M+ EA G IN+ +
Sbjct: 86 EVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSR 145
Query: 386 LFANR 400
+ +++
Sbjct: 146 VISSK 150
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 55.2 bits (127), Expect = 5e-09
Identities = 32/107 (29%), Positives = 59/107 (55%), Gaps = 2/107 (1%)
Frame = +2
Query: 200 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQL 379
S++Q E KEAF L D DKDG+I + + + SLG ++ EL ++ E I+ +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 380 LTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGD 514
++ +N++ E++ I AF+ FD++ G I++ + + T G+
Sbjct: 64 MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLGE 109
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 40.7 bits (91), Expect = 1e-04
Identities = 18/42 (42%), Positives = 26/42 (61%)
Frame = +2
Query: 218 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 343
EF + FQ+ D D G+IG +LR SLG S +E+DE++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 39.9 bits (89), Expect = 2e-04
Identities = 29/103 (28%), Positives = 51/103 (49%), Gaps = 6/103 (5%)
Frame = +2
Query: 200 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 367
+++Q + EAF+L D DKD I ++LRA +LG A + E+ +++ + G +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 368 FTQLLTLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERLR 490
+ + ++ D + + AF+ F DE GKI LR
Sbjct: 92 MEDFVRVMTEKIV-ERDPLEEIKRAFELFDDDETGKISLRNLR 133
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 27.1 bits (57), Expect = 1.7
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = +2
Query: 218 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 343
E +EAF L D G I DLR + LG ++++L M+
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54
>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1274
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +2
Query: 209 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASE 322
+ A+FK+ F DKD + + +L A + L +LA +
Sbjct: 212 EYAKFKDKFPEAPLDKDNLQTQKELEARIECLKQLAEK 249
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,569,778
Number of Sequences: 5004
Number of extensions: 23772
Number of successful extensions: 104
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 103
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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