SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30221
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC9.05 |mfh1||ATP-dependent DNA helicase Mfh1 |Schizosaccharom...    30   0.24 
SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pom...    28   0.95 
SPBC244.01c |sid4||SIN component scaffold protein Sid4 |Schizosa...    26   3.8  
SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar...    26   3.8  
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ...    25   5.1  
SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces p...    25   6.7  
SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit Nse6|Schizosa...    25   8.9  

>SPAC9.05 |mfh1||ATP-dependent DNA helicase Mfh1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 834

 Score = 29.9 bits (64), Expect = 0.24
 Identities = 15/48 (31%), Positives = 27/48 (56%)
 Frame = +3

Query: 108 PSQHLKACQDMVDIPTKSKVTLDCIPARDRMECLNYVQQRQADFVPVD 251
           P   L+A Q+++D    S++    I   + ++   YVQ+++ DF PVD
Sbjct: 230 PGNKLEAIQNVIDSLHISRIE---IRTENSIDISQYVQKKEVDFFPVD 274


>SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 963

 Score = 27.9 bits (59), Expect = 0.95
 Identities = 12/33 (36%), Positives = 16/33 (48%)
 Frame = +1

Query: 232 PISFQLTPKTCTWPPKYPIRTSSFSRSTEPMKN 330
           P+  Q  P+   +PP YPI   S+   T P  N
Sbjct: 736 PVVSQQQPQPYAFPPMYPIPYVSYGYGTMPYNN 768


>SPBC244.01c |sid4||SIN component scaffold protein Sid4
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 660

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 11/42 (26%), Positives = 20/42 (47%)
 Frame = +1

Query: 232 PISFQLTPKTCTWPPKYPIRTSSFSRSTEPMKNRMRLSVTKQ 357
           P  + LT  TC      PI+++   ++ E + N  ++S   Q
Sbjct: 266 PSGYPLTSSTCVSSISQPIQSTDCQKAQENLSNNKQMSSNDQ 307


>SPAC9G1.10c |||inositol polyphosphate phosphatase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1191

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = +1

Query: 271 PPKYPIRTSSFSRSTEPMKN 330
           PPK P+R  S  RS+ P++N
Sbjct: 306 PPKPPLRKVSTQRSSSPIEN 325


>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
           Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 611

 Score = 25.4 bits (53), Expect = 5.1
 Identities = 19/65 (29%), Positives = 29/65 (44%)
 Frame = +1

Query: 109 HHNT*KRVRTWWIFRPNPRSH*TAFPPEIEWSV*ITFSRGKPISFQLTPKTCTWPPKYPI 288
           HH T      WWI+  +PR +    PP     +  T  R   I FQ+ P   T+  +  I
Sbjct: 479 HHKT-----AWWIYVVDPRQNRVIVPPFSITDIPKTL-RTFRIPFQVPPVAGTFSFQLHI 532

Query: 289 RTSSF 303
            ++S+
Sbjct: 533 MSNSY 537


>SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 480

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 8/25 (32%), Positives = 15/25 (60%)
 Frame = -2

Query: 362 NYCFVTERRIRFFIGSVLLENDEVL 288
           NYCF  +R+I   +G +   N +++
Sbjct: 225 NYCFTQKRKITPRLGQITKSNSQIM 249


>SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit
           Nse6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 522

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 6/56 (10%)
 Frame = -1

Query: 432 LQYDRTSSLSAGP--DCLSVNPYG*QLL--LRNGKAHP--VLHRFCTPGKRRSPDW 283
           LQYD T +   G   D L     G  +L  LR+   +   + + +C+  K R PDW
Sbjct: 93  LQYDSTQTFQTGDEIDELIGKSVGSNVLNVLRSNPIYDDDLRYEYCSNSKARVPDW 148


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,343,483
Number of Sequences: 5004
Number of extensions: 49788
Number of successful extensions: 113
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -