BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30221 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC9.05 |mfh1||ATP-dependent DNA helicase Mfh1 |Schizosaccharom... 30 0.24 SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pom... 28 0.95 SPBC244.01c |sid4||SIN component scaffold protein Sid4 |Schizosa... 26 3.8 SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar... 26 3.8 SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ... 25 5.1 SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces p... 25 6.7 SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit Nse6|Schizosa... 25 8.9 >SPAC9.05 |mfh1||ATP-dependent DNA helicase Mfh1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 834 Score = 29.9 bits (64), Expect = 0.24 Identities = 15/48 (31%), Positives = 27/48 (56%) Frame = +3 Query: 108 PSQHLKACQDMVDIPTKSKVTLDCIPARDRMECLNYVQQRQADFVPVD 251 P L+A Q+++D S++ I + ++ YVQ+++ DF PVD Sbjct: 230 PGNKLEAIQNVIDSLHISRIE---IRTENSIDISQYVQKKEVDFFPVD 274 >SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 963 Score = 27.9 bits (59), Expect = 0.95 Identities = 12/33 (36%), Positives = 16/33 (48%) Frame = +1 Query: 232 PISFQLTPKTCTWPPKYPIRTSSFSRSTEPMKN 330 P+ Q P+ +PP YPI S+ T P N Sbjct: 736 PVVSQQQPQPYAFPPMYPIPYVSYGYGTMPYNN 768 >SPBC244.01c |sid4||SIN component scaffold protein Sid4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 660 Score = 25.8 bits (54), Expect = 3.8 Identities = 11/42 (26%), Positives = 20/42 (47%) Frame = +1 Query: 232 PISFQLTPKTCTWPPKYPIRTSSFSRSTEPMKNRMRLSVTKQ 357 P + LT TC PI+++ ++ E + N ++S Q Sbjct: 266 PSGYPLTSSTCVSSISQPIQSTDCQKAQENLSNNKQMSSNDQ 307 >SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1191 Score = 25.8 bits (54), Expect = 3.8 Identities = 10/20 (50%), Positives = 14/20 (70%) Frame = +1 Query: 271 PPKYPIRTSSFSRSTEPMKN 330 PPK P+R S RS+ P++N Sbjct: 306 PPKPPLRKVSTQRSSSPIEN 325 >SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit Sec63 |Schizosaccharomyces pombe|chr 2|||Manual Length = 611 Score = 25.4 bits (53), Expect = 5.1 Identities = 19/65 (29%), Positives = 29/65 (44%) Frame = +1 Query: 109 HHNT*KRVRTWWIFRPNPRSH*TAFPPEIEWSV*ITFSRGKPISFQLTPKTCTWPPKYPI 288 HH T WWI+ +PR + PP + T R I FQ+ P T+ + I Sbjct: 479 HHKT-----AWWIYVVDPRQNRVIVPPFSITDIPKTL-RTFRIPFQVPPVAGTFSFQLHI 532 Query: 289 RTSSF 303 ++S+ Sbjct: 533 MSNSY 537 >SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 480 Score = 25.0 bits (52), Expect = 6.7 Identities = 8/25 (32%), Positives = 15/25 (60%) Frame = -2 Query: 362 NYCFVTERRIRFFIGSVLLENDEVL 288 NYCF +R+I +G + N +++ Sbjct: 225 NYCFTQKRKITPRLGQITKSNSQIM 249 >SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit Nse6|Schizosaccharomyces pombe|chr 1|||Manual Length = 522 Score = 24.6 bits (51), Expect = 8.9 Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 6/56 (10%) Frame = -1 Query: 432 LQYDRTSSLSAGP--DCLSVNPYG*QLL--LRNGKAHP--VLHRFCTPGKRRSPDW 283 LQYD T + G D L G +L LR+ + + + +C+ K R PDW Sbjct: 93 LQYDSTQTFQTGDEIDELIGKSVGSNVLNVLRSNPIYDDDLRYEYCSNSKARVPDW 148 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,343,483 Number of Sequences: 5004 Number of extensions: 49788 Number of successful extensions: 113 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 110 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 113 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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