SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30216
         (505 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 compone...   119   1e-27
At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 compone...   111   3e-25
At4g32120.1 68417.m04570 galactosyltransferase family protein co...    30   0.77 
At2g25300.1 68415.m03026 galactosyltransferase family protein co...    29   1.8  
At1g17450.1 68414.m02137 ATP phosphoribosyltransferase -related ...    27   5.4  
At3g12810.1 68416.m01598 SNF2 domain-containing protein / helica...    27   7.2  
At4g21180.1 68417.m03063 DNAJ heat shock N-terminal domain-conta...    27   9.5  

>At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 component,
           putative / oxoglutarate decarboxylase, putative /
           alpha-ketoglutaric dehydrogenase, putative similar to
           SP|P20967 2-oxoglutarate dehydrogenase E1 component,
           mitochondrial precursor (EC 1.2.4.2)
           (Alpha-ketoglutarate dehydrogenase) {Saccharomyces
           cerevisiae}; contains Pfam profiles PF02779:
           Transketolase, pyridine binding domain, PF00676:
           Dehydrogenase E1 component
          Length = 1025

 Score =  119 bits (286), Expect = 1e-27
 Identities = 63/166 (37%), Positives = 95/166 (57%), Gaps = 1/166 (0%)
 Frame = +3

Query: 9   HNEVDEPMFTQPLMYQKIRKTKPVLEKYADQLIAEGVVTAXXXXXXXXXXXXICEDAYNQ 188
           HNE+DEP FTQP MY+ IR     L+ Y ++L+  G VT             I  + Y  
Sbjct: 505 HNEIDEPSFTQPKMYKVIRSHPSSLQIYQEKLLQSGQVTQEDIDKIQKKVSSILNEEYEA 564

Query: 189 AKQETHIKYKDWLDSPWSGFFEGKDPLKMSATGVVEETLVHIGRRFSSPPPNAAEFEIHK 368
           +K     K +DWL S W+GF   +   ++  TGV  E L ++G+  S+ P N   F+ H+
Sbjct: 565 SKDYIPQK-RDWLASHWTGFKSPEQISRIRNTGVKPEILKNVGKAISTFPEN---FKPHR 620

Query: 369 GLLRILKSRMEMVDN-RTVDWALAEAMAYGSLLKEGIHVRLSGEDV 503
           G+ R+ + R +M+++   +DW L EA+A+ +L+ EG HVRLSG+DV
Sbjct: 621 GVKRVYEQRAQMIESGEGIDWGLGEALAFATLVVEGNHVRLSGQDV 666


>At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 component,
           putative / oxoglutarate decarboxylase, putative /
           alpha-ketoglutaric dehydrogenase, putative similar to
           SP|P20967 2-oxoglutarate dehydrogenase E1 component,
           mitochondrial precursor (EC 1.2.4.2)
           (Alpha-ketoglutarate dehydrogenase) {Saccharomyces
           cerevisiae}; contains Pfam profiles PF02779:
           Transketolase, pyridine binding domain, PF00676:
           Dehydrogenase E1 component
          Length = 1017

 Score =  111 bits (267), Expect = 3e-25
 Identities = 61/166 (36%), Positives = 94/166 (56%), Gaps = 1/166 (0%)
 Frame = +3

Query: 9   HNEVDEPMFTQPLMYQKIRKTKPVLEKYADQLIAEGVVTAXXXXXXXXXXXXICEDAYNQ 188
           HNE+DEP FTQP MY+ I+     L+ Y  +L+  G V+             I  + +  
Sbjct: 501 HNEIDEPSFTQPKMYKVIKNHPSTLQIYHKKLLECGEVSQQDIDRIQEKVNTILNEEFVA 560

Query: 189 AKQETHIKYKDWLDSPWSGFFEGKDPLKMSATGVVEETLVHIGRRFSSPPPNAAEFEIHK 368
           +K     K +DWL + W+GF   +   ++  TGV  E L  +G+  SS P N   F+ H+
Sbjct: 561 SKDYLP-KKRDWLSTNWAGFKSPEQISRVRNTGVKPEILKTVGKAISSLPEN---FKPHR 616

Query: 369 GLLRILKSRMEMVDN-RTVDWALAEAMAYGSLLKEGIHVRLSGEDV 503
            + ++ + R +M+++   VDWALAEA+A+ +L+ EG HVRLSG+DV
Sbjct: 617 AVKKVYEQRAQMIESGEGVDWALAEALAFATLVVEGNHVRLSGQDV 662


>At4g32120.1 68417.m04570 galactosyltransferase family protein
           contains Pfam profile: PF01762 galactosyltransferase
          Length = 345

 Score = 30.3 bits (65), Expect = 0.77
 Identities = 17/35 (48%), Positives = 21/35 (60%)
 Frame = -1

Query: 268 RGSFPSKKPDQGESSQSLYLMWVSCLAWLYASSQI 164
           RG F SK   Q  S  SL L + SCLAWLY + ++
Sbjct: 15  RGRF-SKS--QNTSKPSLILAFFSCLAWLYVAGRL 46


>At2g25300.1 68415.m03026 galactosyltransferase family protein
           contains Pfam profile: PF01762 galactosyltransferase
          Length = 341

 Score = 29.1 bits (62), Expect = 1.8
 Identities = 11/30 (36%), Positives = 18/30 (60%)
 Frame = -1

Query: 253 SKKPDQGESSQSLYLMWVSCLAWLYASSQI 164
           S K  Q  S  S+ + + SC+AWLY + ++
Sbjct: 18  SSKFSQSSSKPSVIMAFFSCVAWLYVAGRL 47


>At1g17450.1 68414.m02137 ATP phosphoribosyltransferase -related
           contains weak similarity to Swiss-Prot:P10366 ATP
           phosphoribosyltransferase [Escherichia coli]
          Length = 1402

 Score = 27.5 bits (58), Expect = 5.4
 Identities = 11/23 (47%), Positives = 15/23 (65%)
 Frame = +3

Query: 357 EIHKGLLRILKSRMEMVDNRTVD 425
           E+HK LL + K R   VD +T+D
Sbjct: 195 ELHKWLLSLEKDRSSKVDRKTID 217


>At3g12810.1 68416.m01598 SNF2 domain-containing protein / helicase
           domain-containing protein similar to transcriptional
           activator SRCAP [Homo sapiens] GI:5106572; contains Pfam
           profiles PF00271: Helicase conserved C-terminal domain,
           PF00176: SNF2 family N-terminal domain
          Length = 2055

 Score = 27.1 bits (57), Expect = 7.2
 Identities = 8/24 (33%), Positives = 17/24 (70%)
 Frame = +3

Query: 198 ETHIKYKDWLDSPWSGFFEGKDPL 269
           ++H ++KDW  +P +G  EG++ +
Sbjct: 716 QSHQEFKDWFCNPIAGMVEGQEKI 739


>At4g21180.1 68417.m03063 DNAJ heat shock N-terminal
           domain-containing protein / sec63 domain-containing
           protein similar to SP|Q9UGP8 Translocation protein SEC63
           homolog {Homo sapiens}; contains Pfam profiles PF00226
           DnaJ domain, PF02889 Sec63 domain
          Length = 661

 Score = 26.6 bits (56), Expect = 9.5
 Identities = 13/47 (27%), Positives = 21/47 (44%)
 Frame = +2

Query: 281 HRCSGGDPRAHRASVLIPPSQRRGVRDPQGAASYPEVAHGDGRQPYS 421
           H     DP A++  V       + + DP    ++ +  H DGRQ Y+
Sbjct: 127 HPDKNPDPEANKYFVESIAKAYQALTDPLSRENFEKYGHPDGRQGYT 173


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,711,094
Number of Sequences: 28952
Number of extensions: 207153
Number of successful extensions: 685
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 666
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 681
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 898188928
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -