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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30210
         (516 letters)

Database: mosquito 
           2352 sequences; 563,979 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY341219-1|AAR13783.1|  200|Anopheles gambiae SRPN10 protein.          48   2e-07
AY341217-1|AAR13781.1|  200|Anopheles gambiae SRPN10 protein.          48   2e-07
AY341216-1|AAR13780.1|  200|Anopheles gambiae SRPN10 protein.          48   2e-07
AY341215-1|AAR13779.1|  200|Anopheles gambiae SRPN10 protein.          48   2e-07
AJ420785-4|CAD12784.1|  395|Anopheles gambiae serpin protein.          48   2e-07
AJ420785-3|CAD12783.1|  380|Anopheles gambiae serpin protein.          48   2e-07
AJ420785-2|CAD12782.1|  382|Anopheles gambiae serpin protein.          48   2e-07
AJ420785-1|CAD12781.1|  379|Anopheles gambiae serpin protein.          48   2e-07
AJ271353-1|CAB69785.1|  380|Anopheles gambiae putative serine pr...    48   2e-07
AJ271352-1|CAB69784.1|  379|Anopheles gambiae putative serine pr...    48   2e-07
AY341218-1|AAR13782.1|  200|Anopheles gambiae SRPN10 protein.          48   2e-07
DQ974169-1|ABJ52809.1|  508|Anopheles gambiae serpin 11 protein.       39   9e-05
DQ974163-1|ABJ52803.1|  595|Anopheles gambiae serpin 4B protein.       38   2e-04
DQ974167-1|ABJ52807.1|  434|Anopheles gambiae serpin 8 protein.        34   0.003
DQ974161-1|ABJ52801.1|  409|Anopheles gambiae serpin 2 protein.        33   0.008
AF203339-1|AAF19834.1|  156|Anopheles gambiae immune-responsive ...    28   0.21 
DQ974166-1|ABJ52806.1|  494|Anopheles gambiae serpin 6 protein.        27   0.50 
DQ974162-1|ABJ52802.1|  418|Anopheles gambiae serpin 3 protein.        25   1.5  
AY753541-1|AAV28544.1| 3398|Anopheles gambiae SGS4 protein.            23   6.1  
AF203336-1|AAF19831.1|  187|Anopheles gambiae immune-responsive ...    23   8.1  

>AY341219-1|AAR13783.1|  200|Anopheles gambiae SRPN10 protein.
          Length = 200

 Score = 48.0 bits (109), Expect = 2e-07
 Identities = 40/139 (28%), Positives = 62/139 (44%), Gaps = 4/139 (2%)
 Frame = +2

Query: 110 SMDSKALSSAITKFSAKFCNELDKK---KNVVSSPLSAEYLLALITLGTTDPAHEELLTS 280
           S+D++ +S +   F+ K    +  K   +NVV SP S    L+L  +G      E++ + 
Sbjct: 52  SLDAQFVSQS-NSFATKLYQRISAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSV 110

Query: 281 LGITDDDTIRXXXXXXXXXXXXIK-GVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADF 457
           L     D  +            +    T NVANKIY+ + +Y +       A   F ++ 
Sbjct: 111 LEFGAPDKKQTVADNYRRLMERLATDSTVNVANKIYVMQ-NYAVKGAFNAIATGSFRSEA 169

Query: 458 EKVDFDNGAAAAGLINKWV 514
           E V+F   AAAA  IN WV
Sbjct: 170 ESVNFAESAAAAKKINGWV 188


>AY341217-1|AAR13781.1|  200|Anopheles gambiae SRPN10 protein.
          Length = 200

 Score = 48.0 bits (109), Expect = 2e-07
 Identities = 40/139 (28%), Positives = 62/139 (44%), Gaps = 4/139 (2%)
 Frame = +2

Query: 110 SMDSKALSSAITKFSAKFCNELDKK---KNVVSSPLSAEYLLALITLGTTDPAHEELLTS 280
           S+D++ +S +   F+ K    +  K   +NVV SP S    L+L  +G      E++ + 
Sbjct: 52  SLDAQFVSQS-NSFATKLYQRISAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSV 110

Query: 281 LGITDDDTIRXXXXXXXXXXXXIK-GVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADF 457
           L     D  +            +    T NVANKIY+ + +Y +       A   F ++ 
Sbjct: 111 LEFGAPDKKQTVADNYRRLMERLATDSTVNVANKIYVMQ-NYAVKGAFNAIATGSFRSEA 169

Query: 458 EKVDFDNGAAAAGLINKWV 514
           E V+F   AAAA  IN WV
Sbjct: 170 ESVNFAESAAAAKKINGWV 188


>AY341216-1|AAR13780.1|  200|Anopheles gambiae SRPN10 protein.
          Length = 200

 Score = 48.0 bits (109), Expect = 2e-07
 Identities = 40/139 (28%), Positives = 62/139 (44%), Gaps = 4/139 (2%)
 Frame = +2

Query: 110 SMDSKALSSAITKFSAKFCNELDKK---KNVVSSPLSAEYLLALITLGTTDPAHEELLTS 280
           S+D++ +S +   F+ K    +  K   +NVV SP S    L+L  +G      E++ + 
Sbjct: 52  SLDAQFVSQS-NSFATKLYQRISAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSV 110

Query: 281 LGITDDDTIRXXXXXXXXXXXXIK-GVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADF 457
           L     D  +            +    T NVANKIY+ + +Y +       A   F ++ 
Sbjct: 111 LEFGAPDKKQTVADNYRRLMERLATDSTVNVANKIYVMQ-NYAVKGAFNAIATGSFRSEA 169

Query: 458 EKVDFDNGAAAAGLINKWV 514
           E V+F   AAAA  IN WV
Sbjct: 170 ESVNFAESAAAAKKINGWV 188


>AY341215-1|AAR13779.1|  200|Anopheles gambiae SRPN10 protein.
          Length = 200

 Score = 48.0 bits (109), Expect = 2e-07
 Identities = 40/139 (28%), Positives = 62/139 (44%), Gaps = 4/139 (2%)
 Frame = +2

Query: 110 SMDSKALSSAITKFSAKFCNELDKK---KNVVSSPLSAEYLLALITLGTTDPAHEELLTS 280
           S+D++ +S +   F+ K    +  K   +NVV SP S    L+L  +G      E++ + 
Sbjct: 52  SLDAQFVSQS-NSFATKLYQRISAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSV 110

Query: 281 LGITDDDTIRXXXXXXXXXXXXIK-GVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADF 457
           L     D  +            +    T NVANKIY+ + +Y +       A   F ++ 
Sbjct: 111 LEFGAPDKKQTVADNYRRLMERLATDSTVNVANKIYVMQ-NYAVKGAFNAIATGSFRSEA 169

Query: 458 EKVDFDNGAAAAGLINKWV 514
           E V+F   AAAA  IN WV
Sbjct: 170 ESVNFAESAAAAKKINGWV 188


>AJ420785-4|CAD12784.1|  395|Anopheles gambiae serpin protein.
          Length = 395

 Score = 48.0 bits (109), Expect = 2e-07
 Identities = 40/139 (28%), Positives = 62/139 (44%), Gaps = 4/139 (2%)
 Frame = +2

Query: 110 SMDSKALSSAITKFSAKFCNELDKK---KNVVSSPLSAEYLLALITLGTTDPAHEELLTS 280
           S+D++ +S +   F+ K    +  K   +NVV SP S    L+L  +G      E++ + 
Sbjct: 7   SLDAQFVSQS-NSFATKLYQRISAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSV 65

Query: 281 LGITDDDTIRXXXXXXXXXXXXIK-GVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADF 457
           L     D  +            +    T NVANKIY+ + +Y +       A   F ++ 
Sbjct: 66  LEFGAPDRKQTVADNYRRLMERLATDSTVNVANKIYVMQ-NYAVKGAFNAIATGSFRSEA 124

Query: 458 EKVDFDNGAAAAGLINKWV 514
           E V+F   AAAA  IN WV
Sbjct: 125 ESVNFAESAAAAKKINGWV 143


>AJ420785-3|CAD12783.1|  380|Anopheles gambiae serpin protein.
          Length = 380

 Score = 48.0 bits (109), Expect = 2e-07
 Identities = 40/139 (28%), Positives = 62/139 (44%), Gaps = 4/139 (2%)
 Frame = +2

Query: 110 SMDSKALSSAITKFSAKFCNELDKK---KNVVSSPLSAEYLLALITLGTTDPAHEELLTS 280
           S+D++ +S +   F+ K    +  K   +NVV SP S    L+L  +G      E++ + 
Sbjct: 7   SLDAQFVSQS-NSFATKLYQRISAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSV 65

Query: 281 LGITDDDTIRXXXXXXXXXXXXIK-GVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADF 457
           L     D  +            +    T NVANKIY+ + +Y +       A   F ++ 
Sbjct: 66  LEFGAPDRKQTVADNYRRLMERLATDSTVNVANKIYVMQ-NYAVKGAFNAIATGSFRSEA 124

Query: 458 EKVDFDNGAAAAGLINKWV 514
           E V+F   AAAA  IN WV
Sbjct: 125 ESVNFAESAAAAKKINGWV 143


>AJ420785-2|CAD12782.1|  382|Anopheles gambiae serpin protein.
          Length = 382

 Score = 48.0 bits (109), Expect = 2e-07
 Identities = 40/139 (28%), Positives = 62/139 (44%), Gaps = 4/139 (2%)
 Frame = +2

Query: 110 SMDSKALSSAITKFSAKFCNELDKK---KNVVSSPLSAEYLLALITLGTTDPAHEELLTS 280
           S+D++ +S +   F+ K    +  K   +NVV SP S    L+L  +G      E++ + 
Sbjct: 7   SLDAQFVSQS-NSFATKLYQRISAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSV 65

Query: 281 LGITDDDTIRXXXXXXXXXXXXIK-GVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADF 457
           L     D  +            +    T NVANKIY+ + +Y +       A   F ++ 
Sbjct: 66  LEFGAPDRKQTVADNYRRLMERLATDSTVNVANKIYVMQ-NYAVKGAFNAIATGSFRSEA 124

Query: 458 EKVDFDNGAAAAGLINKWV 514
           E V+F   AAAA  IN WV
Sbjct: 125 ESVNFAESAAAAKKINGWV 143


>AJ420785-1|CAD12781.1|  379|Anopheles gambiae serpin protein.
          Length = 379

 Score = 48.0 bits (109), Expect = 2e-07
 Identities = 40/139 (28%), Positives = 62/139 (44%), Gaps = 4/139 (2%)
 Frame = +2

Query: 110 SMDSKALSSAITKFSAKFCNELDKK---KNVVSSPLSAEYLLALITLGTTDPAHEELLTS 280
           S+D++ +S +   F+ K    +  K   +NVV SP S    L+L  +G      E++ + 
Sbjct: 7   SLDAQFVSQS-NSFATKLYQRISAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSV 65

Query: 281 LGITDDDTIRXXXXXXXXXXXXIK-GVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADF 457
           L     D  +            +    T NVANKIY+ + +Y +       A   F ++ 
Sbjct: 66  LEFGAPDRKQTVADNYRRLMERLATDSTVNVANKIYVMQ-NYAVKGAFNAIATGSFRSEA 124

Query: 458 EKVDFDNGAAAAGLINKWV 514
           E V+F   AAAA  IN WV
Sbjct: 125 ESVNFAESAAAAKKINGWV 143


>AJ271353-1|CAB69785.1|  380|Anopheles gambiae putative serine
           protease inhibitor protein.
          Length = 380

 Score = 48.0 bits (109), Expect = 2e-07
 Identities = 40/139 (28%), Positives = 62/139 (44%), Gaps = 4/139 (2%)
 Frame = +2

Query: 110 SMDSKALSSAITKFSAKFCNELDKK---KNVVSSPLSAEYLLALITLGTTDPAHEELLTS 280
           S+D++ +S +   F+ K    +  K   +NVV SP S    L+L  +G      E++ + 
Sbjct: 7   SLDAQFVSQS-NSFATKLYQRISAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSV 65

Query: 281 LGITDDDTIRXXXXXXXXXXXXIK-GVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADF 457
           L     D  +            +    T NVANKIY+ + +Y +       A   F ++ 
Sbjct: 66  LEFGAPDRKQTVADNYRRLMERLATDSTVNVANKIYVMQ-NYAVKGAFNAIATGSFRSEA 124

Query: 458 EKVDFDNGAAAAGLINKWV 514
           E V+F   AAAA  IN WV
Sbjct: 125 ESVNFAESAAAAKKINGWV 143


>AJ271352-1|CAB69784.1|  379|Anopheles gambiae putative serine
           protease inhibitor protein.
          Length = 379

 Score = 48.0 bits (109), Expect = 2e-07
 Identities = 40/139 (28%), Positives = 62/139 (44%), Gaps = 4/139 (2%)
 Frame = +2

Query: 110 SMDSKALSSAITKFSAKFCNELDKK---KNVVSSPLSAEYLLALITLGTTDPAHEELLTS 280
           S+D++ +S +   F+ K    +  K   +NVV SP S    L+L  +G      E++ + 
Sbjct: 7   SLDAQFVSQS-NSFATKLYQRISAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSV 65

Query: 281 LGITDDDTIRXXXXXXXXXXXXIK-GVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADF 457
           L     D  +            +    T NVANKIY+ + +Y +       A   F ++ 
Sbjct: 66  LEFGAPDRKQTVADNYRRLMERLATDSTVNVANKIYVMQ-NYAVKGAFNAIATGSFRSEA 124

Query: 458 EKVDFDNGAAAAGLINKWV 514
           E V+F   AAAA  IN WV
Sbjct: 125 ESVNFAESAAAAKKINGWV 143


>AY341218-1|AAR13782.1|  200|Anopheles gambiae SRPN10 protein.
          Length = 200

 Score = 47.6 bits (108), Expect = 2e-07
 Identities = 40/139 (28%), Positives = 62/139 (44%), Gaps = 4/139 (2%)
 Frame = +2

Query: 110 SMDSKALSSAITKFSAKFCNELDKK---KNVVSSPLSAEYLLALITLGTTDPAHEELLTS 280
           S+D++ +S +   F+ K    +  K   +NVV SP S    L+L  +G      E++ + 
Sbjct: 52  SLDAQFVSQS-NSFATKLYQRVSAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSV 110

Query: 281 LGITDDDTIRXXXXXXXXXXXXIK-GVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADF 457
           L     D  +            +    T NVANKIY+ + +Y +       A   F ++ 
Sbjct: 111 LEFGAPDRKQTVADNYRRLMERLATDSTVNVANKIYVMQ-NYAVKGAFNAIATGSFRSEA 169

Query: 458 EKVDFDNGAAAAGLINKWV 514
           E V+F   AAAA  IN WV
Sbjct: 170 ESVNFAESAAAAKKINGWV 188


>DQ974169-1|ABJ52809.1|  508|Anopheles gambiae serpin 11 protein.
          Length = 508

 Score = 39.1 bits (87), Expect = 9e-05
 Identities = 34/144 (23%), Positives = 61/144 (42%), Gaps = 2/144 (1%)
 Frame = +2

Query: 86  LEIKNPVLSMDSKALSSAITKFSAKFCNELDKKKNVVSSPLSAEYLLALITLGTTDPAHE 265
           LE+  P +  D K +  A+  F   F +  D   N + SP+  + LL+ +  G ++    
Sbjct: 117 LEVPKPSVRYDKKVMDFAVKLFQKAFPS--DDTSNYIISPIMVQSLLSYLFDGASNATRL 174

Query: 266 EL--LTSLGITDDDTIRXXXXXXXXXXXXIKGVTFNVANKIYIKEGDYELDPKLKKDAVE 439
           E+  +  L + D   I              K    + A++I+ K   +EL P  + D+++
Sbjct: 175 EMESVLQLNMNDLHDIERALTPQADQEPITKN-KLDSASQIF-KSTTFELLPAFR-DSLK 231

Query: 440 VFDADFEKVDFDNGAAAAGLINKW 511
                  ++DF N   A+  IN W
Sbjct: 232 SNHVPLSEMDFSNPRLASETINNW 255


>DQ974163-1|ABJ52803.1|  595|Anopheles gambiae serpin 4B protein.
          Length = 595

 Score = 38.3 bits (85), Expect = 2e-04
 Identities = 20/60 (33%), Positives = 34/60 (56%), Gaps = 2/60 (3%)
 Frame = +2

Query: 116 DSKALSSAITKFSAKFCNELD--KKKNVVSSPLSAEYLLALITLGTTDPAHEELLTSLGI 289
           ++K +S ++T  +AK  N L   K K  + SP+S    L+L+ LG+     +ELL  +G+
Sbjct: 23  NTKKVSDSVTNLAAKIANALSNQKSKTEIFSPVSIAGALSLLLLGSGGQTQQELLAVMGL 82


>DQ974167-1|ABJ52807.1|  434|Anopheles gambiae serpin 8 protein.
          Length = 434

 Score = 33.9 bits (74), Expect = 0.003
 Identities = 17/41 (41%), Positives = 24/41 (58%)
 Frame = +2

Query: 188 NVVSSPLSAEYLLALITLGTTDPAHEELLTSLGITDDDTIR 310
           N++ SP SA  LL LIT G +    +ELL +L +   + IR
Sbjct: 79  NIIVSPFSAWNLLTLITEGASGRTLDELLVALDVQQQEQIR 119


>DQ974161-1|ABJ52801.1|  409|Anopheles gambiae serpin 2 protein.
          Length = 409

 Score = 32.7 bits (71), Expect = 0.008
 Identities = 36/133 (27%), Positives = 52/133 (39%), Gaps = 10/133 (7%)
 Frame = +2

Query: 146 KFSAKFCNELDKK--KNVVSSPLSAEYLLALI------TLGTTDPAHEELLTSLGITD-- 295
           +F   F  E+ K    NVV SP S + LL LI      + G      +  L+S+   D  
Sbjct: 36  EFDLMFVKEIFKNHNSNVVLSPFSVKILLTLIYEASDTSFGNAVSNTKRELSSVIQNDNI 95

Query: 296 DDTIRXXXXXXXXXXXXIKGVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADFEKVDFD 475
           D T               K    N+A   ++ +   E+  K ++ A   + A  EKV + 
Sbjct: 96  DHTRSYYKQLLESAQQDNKDYDLNIATNFFVDDF-IEVINKYQQIANTHYHAMLEKVSYS 154

Query: 476 NGAAAAGLINKWV 514
           N    A  IN WV
Sbjct: 155 NPTQTAATINNWV 167


>AF203339-1|AAF19834.1|  156|Anopheles gambiae immune-responsive
           serpin-related proteinISerpF1 protein.
          Length = 156

 Score = 27.9 bits (59), Expect = 0.21
 Identities = 16/55 (29%), Positives = 25/55 (45%)
 Frame = +2

Query: 350 KGVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADFEKVDFDNGAAAAGLINKWV 514
           K    N+A   ++ +   E+  K ++ A   + A  EKV + N    A  IN WV
Sbjct: 15  KDYDLNIATNFFVDDF-IEVINKYQQIANTHYHAMLEKVSYSNPTQTAATINNWV 68


>DQ974166-1|ABJ52806.1|  494|Anopheles gambiae serpin 6 protein.
          Length = 494

 Score = 26.6 bits (56), Expect = 0.50
 Identities = 16/55 (29%), Positives = 27/55 (49%)
 Frame = +2

Query: 350 KGVTFNVANKIYIKEGDYELDPKLKKDAVEVFDADFEKVDFDNGAAAAGLINKWV 514
           K     VAN I+ ++ D  +  +    A  ++ A  + +D    AA+  LIN+WV
Sbjct: 196 KSQVIRVANGIFYQK-DLPMRQQYVMLARSLYGALIQPIDPQASAASTALINRWV 249


>DQ974162-1|ABJ52802.1|  418|Anopheles gambiae serpin 3 protein.
          Length = 418

 Score = 25.0 bits (52), Expect = 1.5
 Identities = 26/111 (23%), Positives = 45/111 (40%), Gaps = 2/111 (1%)
 Frame = +2

Query: 188 NVVSSPLSAEYLLALITLGTTDPAH--EELLTSLGITDDDTIRXXXXXXXXXXXXIKGVT 361
           N V SPLS + LLAL+  G+   +    EL  +L   +   +              +   
Sbjct: 63  NAVISPLSVKALLALLYEGSASRSETERELQQALSGGNSQAVPKLQDDLLQYKQQ-QQQN 121

Query: 362 FNVANKIYIKEGDYELDPKLKKDAVEVFDADFEKVDFDNGAAAAGLINKWV 514
             + ++I+  +    L  K        ++A  + VDF +  +AA  IN W+
Sbjct: 122 LLITDRIFY-DTTVTLLQKYHSIIAARYNATTQSVDFQDTQSAAAEINAWI 171


>AY753541-1|AAV28544.1| 3398|Anopheles gambiae SGS4 protein.
          Length = 3398

 Score = 23.0 bits (47), Expect = 6.1
 Identities = 9/21 (42%), Positives = 12/21 (57%)
 Frame = +3

Query: 240  WELLILHTKNY*QA*VSRMMT 302
            W  LI+HTKN+    V  + T
Sbjct: 2972 WSRLIMHTKNFLYVGVRNLQT 2992


>AF203336-1|AAF19831.1|  187|Anopheles gambiae immune-responsive
           chymotrypsin-likeserine protease-related protein ISPR1
           protein.
          Length = 187

 Score = 22.6 bits (46), Expect = 8.1
 Identities = 10/39 (25%), Positives = 21/39 (53%)
 Frame = -1

Query: 360 VTPFIDFNFDDTAENDERIVSSSVIPRLVNSSSCAGSVV 244
           ++PF      D ++ +E      V  ++  +S+C+GS+V
Sbjct: 33  ISPFAANYIVDGSDAEENAAPYQVSLQIDGNSTCSGSIV 71


  Database: mosquito
    Posted date:  Oct 23, 2007  1:18 PM
  Number of letters in database: 563,979
  Number of sequences in database:  2352
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 467,882
Number of Sequences: 2352
Number of extensions: 8686
Number of successful extensions: 39
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28
length of database: 563,979
effective HSP length: 60
effective length of database: 422,859
effective search space used: 46937349
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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