Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30200
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17440.2 68414.m02133 transcription initiation factor IID (TF... 93 8e-20
At1g17440.1 68414.m02132 transcription initiation factor IID (TF... 93 8e-20
At3g10070.1 68416.m01207 transcription initiation factor IID (TF... 88 4e-18
At5g02880.1 68418.m00231 HECT-domain-containing protein / ubiqui... 29 1.4
At3g50230.1 68416.m05493 leucine-rich repeat transmembrane prote... 29 1.4
At1g67140.1 68414.m07638 expressed protein 29 1.4
At5g51540.1 68418.m06391 peptidase M3 family protein / thimet ol... 29 2.5
At2g16470.1 68415.m01887 zinc finger (CCCH-type) family protein ... 28 4.3
At1g59700.1 68414.m06716 glutathione S-transferase, putative sim... 27 5.7
At3g10760.1 68416.m01295 myb family transcription factor contain... 27 7.5
At2g44570.1 68415.m05547 glycosyl hydrolase family 9 protein 27 7.5
At1g21080.1 68414.m02637 DNAJ heat shock N-terminal domain-conta... 27 7.5
At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes... 27 7.5
At5g05090.1 68418.m00540 myb family transcription factor contain... 27 9.9
At2g35270.1 68415.m04326 DNA-binding protein-related contains Pf... 27 9.9
>At1g17440.2 68414.m02133 transcription initiation factor IID
(TFIID) subunit A family protein similar to SP|Q16514
Transcription initiation factor TFIID 20/15 kDa subunits
(TAFII-20/TAFII-15) {Homo sapiens}; contains Pfam
profile PF03847: Transcription initiation factor TFIID
subunit A
Length = 683
Score = 93.5 bits (222), Expect = 8e-20
Identities = 47/130 (36%), Positives = 83/130 (63%), Gaps = 6/130 (4%)
Frame = +1
Query: 142 VNNPMQSPQL------QNTSIQGSPSQHSPMGTQSQVAKVGQGGAGDQSSQLLSRPRLQE 303
+N SP++ ++ S+ GS + + GT + QG + ++QLL + ++Q+
Sbjct: 478 INQQQPSPRMLSHAGQKSVSLTGSQPEATQSGTTTPGGSSSQG--TEATNQLLGKRKIQD 535
Query: 304 LVREVDPTVQLDEEVEEMLLQLADDFIDTTLNSACALAKHRHAPNVELRDVQLHLERQWN 483
LV +VD +LD +VE++LL++ADDFID+ + AC+LAKHR + +E +D+ LHLE+ +
Sbjct: 536 LVSQVDVHAKLDPDVEDLLLEVADDFIDSVTSFACSLAKHRKSSVLEPKDILLHLEKNLH 595
Query: 484 MWIPGFGNDE 513
+ IPGF +++
Sbjct: 596 LTIPGFSSED 605
>At1g17440.1 68414.m02132 transcription initiation factor IID
(TFIID) subunit A family protein similar to SP|Q16514
Transcription initiation factor TFIID 20/15 kDa subunits
(TAFII-20/TAFII-15) {Homo sapiens}; contains Pfam
profile PF03847: Transcription initiation factor TFIID
subunit A
Length = 683
Score = 93.5 bits (222), Expect = 8e-20
Identities = 47/130 (36%), Positives = 83/130 (63%), Gaps = 6/130 (4%)
Frame = +1
Query: 142 VNNPMQSPQL------QNTSIQGSPSQHSPMGTQSQVAKVGQGGAGDQSSQLLSRPRLQE 303
+N SP++ ++ S+ GS + + GT + QG + ++QLL + ++Q+
Sbjct: 478 INQQQPSPRMLSHAGQKSVSLTGSQPEATQSGTTTPGGSSSQG--TEATNQLLGKRKIQD 535
Query: 304 LVREVDPTVQLDEEVEEMLLQLADDFIDTTLNSACALAKHRHAPNVELRDVQLHLERQWN 483
LV +VD +LD +VE++LL++ADDFID+ + AC+LAKHR + +E +D+ LHLE+ +
Sbjct: 536 LVSQVDVHAKLDPDVEDLLLEVADDFIDSVTSFACSLAKHRKSSVLEPKDILLHLEKNLH 595
Query: 484 MWIPGFGNDE 513
+ IPGF +++
Sbjct: 596 LTIPGFSSED 605
>At3g10070.1 68416.m01207 transcription initiation factor IID
(TFIID) subunit A family protein similar to hypothetical
protein GB:CAB10099 [Schizosaccharomyces pombe];
contains Pfam profile PF03847: Transcription initiation
factor TFIID subunit A
Length = 539
Score = 87.8 bits (208), Expect = 4e-18
Identities = 43/132 (32%), Positives = 74/132 (56%), Gaps = 2/132 (1%)
Frame = +1
Query: 124 QGAIQYVNNPMQSPQLQNTSIQGS--PSQHSPMGTQSQVAKVGQGGAGDQSSQLLSRPRL 297
Q + + + P + L N + PSQ P+ AK ++L + +
Sbjct: 347 QQPLAHPHQPTRVQGLVNQKVTSPVMPSQ-PPVAQPGNHAKTVSAETEPSDDRILGKRSI 405
Query: 298 QELVREVDPTVQLDEEVEEMLLQLADDFIDTTLNSACALAKHRHAPNVELRDVQLHLERQ 477
EL++++DP+ +LD EVE++L +A+DF+++ C+LAKHR + +E +D+ LH+ER
Sbjct: 406 HELLQQIDPSEKLDPEVEDILSDIAEDFVESITTFGCSLAKHRKSDILEAKDILLHVERN 465
Query: 478 WNMWIPGFGNDE 513
WN+ PGF +DE
Sbjct: 466 WNIRPPGFSSDE 477
>At5g02880.1 68418.m00231 HECT-domain-containing protein /
ubiquitin-transferase family protein /
armadillo/beta-catenin-like repeat-containing protein
similar to SP|Q14669 Thyroid receptor interacting protein
12 (TRIP12) {Homo sapiens}; contains Pfam profiles
PF00632: HECT-domain (ubiquitin-transferase), PF00514:
Armadillo/beta-catenin-like repeat
Length = 1502
Score = 29.5 bits (63), Expect = 1.4
Identities = 25/92 (27%), Positives = 43/92 (46%), Gaps = 2/92 (2%)
Frame = +1
Query: 157 QSPQLQNTSIQGSPSQHSPMGTQSQVAKVGQGGAGDQSSQLLSRPRL--QELVREVDPTV 330
QS QLQ+TSI SPM S+ + Q Q SQ+ + +L Q+ + +
Sbjct: 822 QSSQLQSTSISCQAESSSPMEIDSESSDASQ----LQGSQVEDQTQLPGQQNASSSETSS 877
Query: 331 QLDEEVEEMLLQLADDFIDTTLNSACALAKHR 426
+ ++ V +L +L +D +L A+ H+
Sbjct: 878 EKEDAVPRLLFRLEGLELDRSLTVYQAILLHK 909
>At3g50230.1 68416.m05493 leucine-rich repeat transmembrane protein
kinase, putative receptor-like protein kinase (RKL1),
Arabidopsis thaliana, EMBL:AF084034
Length = 660
Score = 29.5 bits (63), Expect = 1.4
Identities = 18/46 (39%), Positives = 23/46 (50%)
Frame = +1
Query: 169 LQNTSIQGSPSQHSPMGTQSQVAKVGQGGAGDQSSQLLSRPRLQEL 306
L+N SI GS SP+ + G +G SS +LS RL EL
Sbjct: 106 LENNSISGSIPDLSPLVNLKTLTLSKNGFSGTLSSSILSLRRLTEL 151
>At1g67140.1 68414.m07638 expressed protein
Length = 2158
Score = 29.5 bits (63), Expect = 1.4
Identities = 15/41 (36%), Positives = 25/41 (60%)
Frame = +1
Query: 280 LSRPRLQELVREVDPTVQLDEEVEEMLLQLADDFIDTTLNS 402
LS L+ LV E DP +DE++E+ L Q+ D+ D+ + +
Sbjct: 982 LSVSTLRHLV-EKDPVSVIDEQIEDNLFQMLDEETDSEIGN 1021
>At5g51540.1 68418.m06391 peptidase M3 family protein / thimet
oligopeptidase family protein low similarity to
SP|Q99797 Mitochondrial intermediate peptidase,
mitochondrial precursor (EC 3.4.24.59) {Homo sapiens};
contains Pfam profile PF01432: Peptidase family M3
Length = 860
Score = 28.7 bits (61), Expect = 2.5
Identities = 16/64 (25%), Positives = 25/64 (39%)
Frame = +1
Query: 82 LAQAANMPTIGTVGQGAIQYVNNPMQSPQLQNTSIQGSPSQHSPMGTQSQVAKVGQGGAG 261
L A I G+G + NP + + QN+ I+ ++ S + VG G
Sbjct: 677 LTDLAGKEIISVHGEGIVPATTNPRFAIKCQNSQIESDTTEDPSRSKNSSSSGVGFGSPA 736
Query: 262 DQSS 273
SS
Sbjct: 737 SSSS 740
>At2g16470.1 68415.m01887 zinc finger (CCCH-type) family protein /
GYF domain-containing protein contains Pfam domains
PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and
similar), PF02213: GYF domain
Length = 659
Score = 27.9 bits (59), Expect = 4.3
Identities = 22/73 (30%), Positives = 33/73 (45%), Gaps = 4/73 (5%)
Frame = +1
Query: 70 SNNSLAQAANMPTIGTVGQGAIQYVNNPMQSPQLQNTSIQGSPSQHSPM---GTQSQVA- 237
S S ++P + GA+Q P+ +P N S+ S + HSP G QS +
Sbjct: 350 SGQSQTSRIDIPVVVN-SAGALQPQTYPIPTPDPINVSVNHSATLHSPTPAGGKQSWGSM 408
Query: 238 KVGQGGAGDQSSQ 276
+ GG+ SSQ
Sbjct: 409 QTDHGGSNTPSSQ 421
>At1g59700.1 68414.m06716 glutathione S-transferase, putative
similar to glutathione S-transferase GB:AAF29773
GI:6856103 from [Gossypium hirsutum]
Length = 234
Score = 27.5 bits (58), Expect = 5.7
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +1
Query: 292 RLQELVREVDPTVQLDEEVEEMLLQLADDFI 384
R+ + + D + EEVEE LLQL D F+
Sbjct: 115 RMAAITKSEDAKAKAMEEVEEGLLQLEDAFV 145
>At3g10760.1 68416.m01295 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 335
Score = 27.1 bits (57), Expect = 7.5
Identities = 33/99 (33%), Positives = 43/99 (43%), Gaps = 7/99 (7%)
Frame = +1
Query: 151 PMQSPQLQNTSIQGSPSQHSPMGTQSQVAKVGQGGA-GDQSSQLLSRPRL----QELVRE 315
P QLQ S Q + S + A +G GGA GD+ ++ L RPRL Q R
Sbjct: 63 PPTPSQLQLPSSQANSSAEFAADS----ADLGSGGAAGDEPARTLKRPRLVWTPQLHKRF 118
Query: 316 VDPTVQL--DEEVEEMLLQLADDFIDTTLNSACALAKHR 426
VD L V + ++QL T N A L K+R
Sbjct: 119 VDAVAHLGIKNAVPKTIMQLMSVDGLTRENVASHLQKYR 157
>At2g44570.1 68415.m05547 glycosyl hydrolase family 9 protein
Length = 492
Score = 27.1 bits (57), Expect = 7.5
Identities = 14/49 (28%), Positives = 21/49 (42%)
Frame = +1
Query: 79 SLAQAANMPTIGTVGQGAIQYVNNPMQSPQLQNTSIQGSPSQHSPMGTQ 225
S+ N P T G + NNP +P L +I G P ++ G +
Sbjct: 419 SIVSIKNDPKPVTCNGGFEAWYNNPKPNPNLLVGAIVGGPDEYDAYGDE 467
>At1g21080.1 68414.m02637 DNAJ heat shock N-terminal
domain-containing protein similar to SP|P39101 CAJ1
protein [Saccharomyces cerevisiae]; contains Pfam
profile PF00226 DnaJ domain;
Length = 391
Score = 27.1 bits (57), Expect = 7.5
Identities = 18/74 (24%), Positives = 31/74 (41%), Gaps = 1/74 (1%)
Frame = +1
Query: 85 AQAANMPTIGTVGQ-GAIQYVNNPMQSPQLQNTSIQGSPSQHSPMGTQSQVAKVGQGGAG 261
A+A + T+G + Q I ++P+ +L + G HS +S A G
Sbjct: 298 ARAKGLKTLGKIFQKNKIASESDPLVRAELHKLNGNGQEHDHSSTSPKSDEASRSTVGPQ 357
Query: 262 DQSSQLLSRPRLQE 303
+ S + P+L E
Sbjct: 358 EPQSPYVEAPKLGE 371
>At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes
from this gene
Length = 1035
Score = 27.1 bits (57), Expect = 7.5
Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 3/60 (5%)
Frame = +1
Query: 295 LQELVREVDPTVQLDEEVEEMLLQLADDFIDTTL-NSAC--ALAKHRHAPNVELRDVQLH 465
++ + E++ T + +EE MLL+LA + D L S C + K H + V LH
Sbjct: 500 IENMKAEIEETEEGEEEAATMLLRLAKETEDLALRESECFSPILKRWHLVAAGVASVSLH 559
>At5g05090.1 68418.m00540 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 266
Score = 26.6 bits (56), Expect = 9.9
Identities = 26/90 (28%), Positives = 37/90 (41%), Gaps = 6/90 (6%)
Frame = +1
Query: 175 NTSIQGSPSQHSPMGTQSQVAKVGQGGAGDQSSQLLSRPRL----QELVREVDPTVQL-- 336
N S G P + + A++ GD+ ++ L RPRL Q R VD L
Sbjct: 44 NNSNSGQPLPQTTPPQPNSSAEIAGDSTGDEPARTLKRPRLVWTPQLHKRFVDAVAHLGI 103
Query: 337 DEEVEEMLLQLADDFIDTTLNSACALAKHR 426
V + ++QL T N A L K+R
Sbjct: 104 KNAVPKTIMQLMSVDGLTRENVASHLQKYR 133
>At2g35270.1 68415.m04326 DNA-binding protein-related contains Pfam
domain PF03479: Domain of unknown function (DUF296),
found in AT-hook motifs Pfam:PF02178
Length = 285
Score = 26.6 bits (56), Expect = 9.9
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +1
Query: 97 NMPTIGTVGQGAIQYVNNPMQSPQLQNTSIQGS 195
NM + T G G + + N PM PQ+ S QG+
Sbjct: 244 NMYSEATGGGGGLPFFNLPMSMPQIGVESWQGN 276
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,752,889
Number of Sequences: 28952
Number of extensions: 170712
Number of successful extensions: 493
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 483
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 492
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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