Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30198
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 237 5e-64
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 225 4e-60
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 28 0.95
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 27 1.7
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 26 2.9
SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 5.1
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 25 6.7
SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schiz... 25 8.9
SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce... 25 8.9
SPBC11C11.07 |rpl1801|rpl18-1, rpl18|60S ribosomal protein L18|S... 25 8.9
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 237 bits (581), Expect = 5e-64
Identities = 110/157 (70%), Positives = 132/157 (84%)
Frame = +2
Query: 8 QMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGE 187
++ KEYR K+E+EL IC D+L +L+KHLIP A++ ESKVFY KMKGDYYRYLAE A GE
Sbjct: 82 ELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGE 141
Query: 188 TRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAF 367
R + S + Y+ A EI+ A++ PTHPIRLGLALNFSVFYYEILNSPD+AC LAKQAF
Sbjct: 142 KRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAF 201
Query: 368 DDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDTQ 478
D+AI+ELD+L+E+SYKDSTLIMQLLRDNLTLWTSD +
Sbjct: 202 DEAISELDSLSEESYKDSTLIMQLLRDNLTLWTSDAE 238
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 225 bits (549), Expect = 4e-60
Identities = 104/156 (66%), Positives = 127/156 (81%)
Frame = +2
Query: 11 MAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGET 190
+ KEYR K+E EL +IC+DVL +L+KHLIP A+ ESKVFY KMKGDYYRYLAE GE
Sbjct: 82 LIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKVFYYKMKGDYYRYLAEFTVGEV 141
Query: 191 RHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFD 370
+ S +AY+ A +I+ A++ PT P+RLGLALNFSVFYYEIL+SP+ AC LAKQ FD
Sbjct: 142 CKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFSVFYYEILDSPESACHLAKQVFD 201
Query: 371 DAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDTQ 478
+AI+ELD+L+E+SYKDSTLIMQLLRDNLTLWTSD +
Sbjct: 202 EAISELDSLSEESYKDSTLIMQLLRDNLTLWTSDAE 237
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1207
Score = 27.9 bits (59), Expect = 0.95
Identities = 29/103 (28%), Positives = 51/103 (49%), Gaps = 4/103 (3%)
Frame = +2
Query: 8 QMAKEYR-VKVEKELR-EICYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVAT 181
++ + YR VK K L +IC+ + L L A++ E + + RY+ ++
Sbjct: 999 ELHEAYRLVKANKILEAQICFRSIIYLA--LTTVANSEEEADEISALIDECCRYIVALSC 1056
Query: 182 G-ETRHSVVEDSQKAYQDAFEISKAKMQPTHP-IRLGLALNFS 304
E R ED+++A + ++ + A +QP H I L LA+N S
Sbjct: 1057 ELERRRLGEEDTKRALELSYYFASADLQPMHSIIALRLAINAS 1099
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 27.1 bits (57), Expect = 1.7
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = +2
Query: 77 LLDKHLIPKASNPESKVFYLKMKGDYYR---YLAEVATGETRHSVVEDS 214
+++K IP++ E+K Y + GD+ +L E A E H V+ D+
Sbjct: 1608 MIEKLCIPESWLNEAKALYARYVGDHLNELYFLQEAALYEDAHKVLLDT 1656
>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 817
Score = 26.2 bits (55), Expect = 2.9
Identities = 20/81 (24%), Positives = 34/81 (41%), Gaps = 2/81 (2%)
Frame = +2
Query: 215 QKAYQDAFEISKAKMQPTHPIRL-GLALNF-SVFYYEILNSPDKACQLAKQAFDDAIAEL 388
Q+ D E + P H L F S Y ++ D QLA Q F ++
Sbjct: 415 QRLVADLHEFYNSVKGPAHSFETEDPVLKFVSQSYDDLFPYMDNFIQLAVQLFYHISKKV 474
Query: 389 DTLNEDSYKDSTLIMQLLRDN 451
+ L +++ S ++Q ++DN
Sbjct: 475 NCLYVEAFSSSDAVLQRIQDN 495
>SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 85
Score = 25.4 bits (53), Expect = 5.1
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -3
Query: 268 CGLHFRLADFKSILVCFL*ILYNRMSGFSCGHFC 167
C L+F L + K+ L+ I+Y + GFS H C
Sbjct: 46 CLLNFSLRENKNYLI----IVYLPIEGFSANHMC 75
>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 971
Score = 25.0 bits (52), Expect = 6.7
Identities = 13/42 (30%), Positives = 18/42 (42%)
Frame = +2
Query: 89 HLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDS 214
H SNP S K KG++ Y A G ++ +DS
Sbjct: 225 HTSKTYSNPSSFATRKKEKGEHLSYAEAAAVGTQAKNIKKDS 266
>SPAC3A11.05c |kms1||meiotic spindle pole body protein
Kms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 607
Score = 24.6 bits (51), Expect = 8.9
Identities = 14/40 (35%), Positives = 19/40 (47%)
Frame = +2
Query: 83 DKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSV 202
+KHLI ++NP+ F D Y EV T+ SV
Sbjct: 354 EKHLIALSNNPKGDEFLSPSNLDEMVYSKEVGLSFTQPSV 393
>SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 844
Score = 24.6 bits (51), Expect = 8.9
Identities = 16/36 (44%), Positives = 21/36 (58%), Gaps = 3/36 (8%)
Frame = +2
Query: 68 VLGLLDKHLIPKASN-PESKVFYLKMKGD--YYRYL 166
VL L+ L+ A+ PE K+FY K GD Y +YL
Sbjct: 509 VLMQLESFLLNIANPAPEGKLFYEKQTGDNPYLKYL 544
>SPBC11C11.07 |rpl1801|rpl18-1, rpl18|60S ribosomal protein
L18|Schizosaccharomyces pombe|chr 2|||Manual
Length = 187
Score = 24.6 bits (51), Expect = 8.9
Identities = 12/32 (37%), Positives = 20/32 (62%), Gaps = 2/32 (6%)
Frame = +2
Query: 80 LDKHLIPKA--SNPESKVFYLKMKGDYYRYLA 169
+++H + K+ S P S+ YLK+ YR+LA
Sbjct: 5 IERHHVKKSQRSKPASENVYLKLLVKLYRFLA 36
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,984,654
Number of Sequences: 5004
Number of extensions: 40222
Number of successful extensions: 136
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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