SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30190
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC30D10.15 |||snoRNP assembly factor |Schizosaccharomyces pomb...    29   0.41 
SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 compon...    29   0.55 
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    27   1.3  
SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Sc...    27   1.3  
SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces...    27   1.3  
SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium tra...    27   2.2  
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    26   2.9  
SPCC24B10.15 |||PINc domain|Schizosaccharomyces pombe|chr 3|||Ma...    26   3.8  
SPAC18B11.10 |tup11||transcriptional corepressor Tup11|Schizosac...    26   3.8  
SPBC582.05c |brc1||BRCT domain protein Brc1|Schizosaccharomyces ...    26   3.8  
SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc...    26   3.8  
SPAC3A12.17c |cys12|cys1b|cysteine synthase Cys12|Schizosaccharo...    25   5.1  
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb...    25   5.1  
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    25   5.1  
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M...    25   6.7  
SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2||...    25   6.7  
SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyce...    25   6.7  
SPBC651.09c |||RNA polymerase II associated Paf1 complex |Schizo...    25   8.9  
SPAC1142.08 |fhl1|SPAC8C9.01|fork head transcription factor Fhl1...    25   8.9  
SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces p...    25   8.9  
SPAC25B8.19c ||SPAC683.01c|transcription factor, zf-fungal binuc...    25   8.9  

>SPBC30D10.15 |||snoRNP assembly factor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 516

 Score = 29.1 bits (62), Expect = 0.41
 Identities = 29/103 (28%), Positives = 43/103 (41%), Gaps = 4/103 (3%)
 Frame = +1

Query: 94  VSNWP-YRTTPLVLPGAK-VRREPGPTESYLRHHP--NPAMRAPPNHDYRDTLMKQKVLH 261
           V N+P Y+T     P AK   +EP  +  Y       N + ++P  ++Y          H
Sbjct: 409 VRNYPFYQTNQGSNPPAKRFTQEPPSSSLYSLSESSINYSTQSPMYYNYNYPQPSFPPFH 468

Query: 262 KQFNSPINLYSEQNIANSIRQQTSPLPPRPAAQYDPAKSETYR 390
             +N  I  YS+ N       Q S  PP+    +DP  S+ YR
Sbjct: 469 PIYNDSIGYYSQANPQMYYANQVSAPPPQ--GSFDP-NSKFYR 508


>SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 component
           of oxoglutarate dehydrogenase complex
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 452

 Score = 28.7 bits (61), Expect = 0.55
 Identities = 18/48 (37%), Positives = 27/48 (56%), Gaps = 5/48 (10%)
 Frame = +1

Query: 379 ETYRALQEDGLPDAATEL--SAPVATKVFTAPTSKRP---APTPKPTK 507
           ++ + ++E  +PD   E   SAP +TK   AP +K P   +P PKP K
Sbjct: 151 DSSKPIEEKPMPDLGAEQKESAPSSTK--PAPDAKEPEFSSPKPKPAK 196


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 18/65 (27%), Positives = 28/65 (43%), Gaps = 1/65 (1%)
 Frame = +1

Query: 319  RQQTSPLP-PRPAAQYDPAKSETYRALQEDGLPDAATELSAPVATKVFTAPTSKRPAPTP 495
            RQ+ +P P P  +A   P  + T + +Q      A +     V+  + T P        P
Sbjct: 1253 RQKGTPSPAPVNSATSTPVAAPTAQQIQPGKQASAVSSNVPAVSASISTPPAVVPTVQHP 1312

Query: 496  KPTKQ 510
            +PTKQ
Sbjct: 1313 QPTKQ 1317



 Score = 25.0 bits (52), Expect = 6.7
 Identities = 17/79 (21%), Positives = 28/79 (35%), Gaps = 3/79 (3%)
 Frame = +1

Query: 274  SPINLYSEQNIANSIRQQTSPLPPRPAAQYDPAKSETYRALQEDGLPDAATELSAPVATK 453
            +P+   +   + N+    ++P PP P +Q  PA            +        AP    
Sbjct: 1456 APVQPKAPGMVTNAPAPSSAPAPPAPVSQLPPAVPNVPVPSMIPSVAQQPPSSVAPATAP 1515

Query: 454  VFTAPTSKRP---APTPKP 501
              T P S+      P+P P
Sbjct: 1516 SSTLPPSQSSFAHVPSPAP 1534


>SPBC428.07 |meu6||meiotic chromosome segregation protein
           Meu6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 651

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 20/72 (27%), Positives = 32/72 (44%), Gaps = 6/72 (8%)
 Frame = +1

Query: 211 PPNHDYRDTLMKQKVLHKQFNSPINLYSEQNIANSIRQQTSPLPP------RPAAQYDPA 372
           P N   + + + QK +HK+F+    L      + +  +  +PL P      R AAQ DPA
Sbjct: 552 PSNLSKKLSGLVQKKVHKKFDKDGRLKEISQFSKTTIKPETPLTPTTTPTPRTAAQEDPA 611

Query: 373 KSETYRALQEDG 408
           +  T      +G
Sbjct: 612 EETTDALASAEG 623


>SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 466

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 19/69 (27%), Positives = 25/69 (36%)
 Frame = +1

Query: 289 YSEQNIANSIRQQTSPLPPRPAAQYDPAKSETYRALQEDGLPDAATELSAPVATKVFTAP 468
           YS  +      Q T+      AAQY  A       + +       T ++APVA      P
Sbjct: 336 YSTPSAGYQTVQTTTTTTEAAAAQYPQAAFPPPPVMPQPAAAAVTTPVAAPVAAAAAAVP 395

Query: 469 TSKRPAPTP 495
               PAP P
Sbjct: 396 VPP-PAPAP 403


>SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium
            transporting Cta4 |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1211

 Score = 26.6 bits (56), Expect = 2.2
 Identities = 14/60 (23%), Positives = 26/60 (43%), Gaps = 1/60 (1%)
 Frame = +1

Query: 280  INLYSEQ-NIANSIRQQTSPLPPRPAAQYDPAKSETYRALQEDGLPDAATELSAPVATKV 456
            + +Y +Q  +A      T P+PP     + P  +  +R   ++GL     +L    A+ V
Sbjct: 862  MGVYEKQIQLAKRFNLPTPPVPPALCHAFPPGPNNPHREKTQEGLNKVLEDLETKKASDV 921


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 26.2 bits (55), Expect = 2.9
 Identities = 25/120 (20%), Positives = 40/120 (33%)
 Frame = +1

Query: 157 PGPTESYLRHHPNPAMRAPPNHDYRDTLMKQKVLHKQFNSPINLYSEQNIANSIRQQTSP 336
           P PT+SY +++  P          R     +K   K F  P     +  + +     ++P
Sbjct: 72  PHPTKSYQQYYQKPTGNTVDEDKSRVPDFSKK---KSFVPPKPAIPKGKVLSLGGNTSAP 128

Query: 337 LPPRPAAQYDPAKSETYRALQEDGLPDAATELSAPVATKVFTAPTSKRPAPTPKPTKQSD 516
              +P +                    + TE  AP  T   T   +  P P   PTK +D
Sbjct: 129 KSTKPISISLGGTKAPTTTKPAAPAAQSKTETPAPKVTSESTKKETAAPPPQETPTKSAD 188


>SPCC24B10.15 |||PINc domain|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 462

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 23/86 (26%), Positives = 32/86 (37%)
 Frame = -1

Query: 264 LVQHLLFHKGITVVVVRRCPHCWVGVMTQVTLCGARLPSDLSSREHERSGAVRPVADNFF 85
           L+ HL   + +   +  RCP   V V+    L   +    L S      G +   A NF 
Sbjct: 76  LLSHLSLCQNLIEFLTARCPRLVV-VLPWTVL---QELDGLKSESSSTCGYLARQAHNFL 131

Query: 84  FRMFRILVSVREEVGVERHCYTCRYG 7
            + FR  VS      V  HC +   G
Sbjct: 132 LQCFRSNVSSLRGQKVHEHCSSTEKG 157


>SPAC18B11.10 |tup11||transcriptional corepressor
           Tup11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 614

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 18/70 (25%), Positives = 29/70 (41%), Gaps = 1/70 (1%)
 Frame = +1

Query: 10  IPTSVTMSLNPNFFPN-GYQDPKHPEEEVVSNWPYRTTPLVLPGAKVRREPGPTESYLRH 186
           IP SV  S   NF      Q+P      + S  P    P+     +V ++P P    + +
Sbjct: 159 IPPSVEASSGQNFNQGIASQNPAISTSNLPSTTPLYIPPVNYGANQVSQQPNPQLPGVSN 218

Query: 187 HPNPAMRAPP 216
           + NP+  + P
Sbjct: 219 YYNPSATSKP 228


>SPBC582.05c |brc1||BRCT domain protein Brc1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 878

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 20/74 (27%), Positives = 29/74 (39%), Gaps = 1/74 (1%)
 Frame = +1

Query: 286 LYSEQNIANSIRQQTSPLPPRPAAQYDPAKSETYRALQEDGLPDAATEL-SAPVATKVFT 462
           L  E  +    +   +  PP P     P+K     A  +D LP  AT+L    +  K   
Sbjct: 510 LQDEGRLEIDAKSSKTNTPPSPLLVGTPSKESLKEASSDDELPVLATKLVDNVIKEKSPL 569

Query: 463 APTSKRPAPTPKPT 504
           + T K   P+ K T
Sbjct: 570 SLTPKVVVPSHKET 583


>SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase
           Cdc7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1062

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 14/49 (28%), Positives = 21/49 (42%)
 Frame = +1

Query: 289 YSEQNIANSIRQQTSPLPPRPAAQYDPAKSETYRALQEDGLPDAATELS 435
           ++   I+ S     SPL     AQ+DP+K    R++     P     LS
Sbjct: 377 FNSDQISESNNFNASPLSTPLKAQFDPSKPALNRSIDHQKTPQHKRYLS 425


>SPAC3A12.17c |cys12|cys1b|cysteine synthase
           Cys12|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 395

 Score = 25.4 bits (53), Expect = 5.1
 Identities = 19/61 (31%), Positives = 26/61 (42%), Gaps = 1/61 (1%)
 Frame = +1

Query: 124 LVLPGAKVRR-EPGPTESYLRHHPNPAMRAPPNHDYRDTLMKQKVLHKQFNSPINLYSEQ 300
           L L GA V+R  P P      H  N A R   NH   +++  +     QF +P N  +  
Sbjct: 147 LELLGAHVQRVTPAPIVDP-NHFVNTARRNAANHTVDESIPGKGYFANQFENPANWQAHF 205

Query: 301 N 303
           N
Sbjct: 206 N 206


>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1334

 Score = 25.4 bits (53), Expect = 5.1
 Identities = 15/54 (27%), Positives = 23/54 (42%)
 Frame = +1

Query: 106 PYRTTPLVLPGAKVRREPGPTESYLRHHPNPAMRAPPNHDYRDTLMKQKVLHKQ 267
           P    PL  P  ++ R+P P  S     P+    APP H  R+    + + H +
Sbjct: 44  PVSKKPLPPPTRRLPRKPLPFRSTSLQPPSSQPPAPPTHQ-REASPVKNIEHSE 96


>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 574

 Score = 25.4 bits (53), Expect = 5.1
 Identities = 16/58 (27%), Positives = 27/58 (46%)
 Frame = +1

Query: 340 PPRPAAQYDPAKSETYRALQEDGLPDAATELSAPVATKVFTAPTSKRPAPTPKPTKQS 513
           PPRP A    + +    +  +  LP  ++ +SA        A   KRP P P P++++
Sbjct: 271 PPRPIAPV--SMNPAINSTSKPPLPPPSSRVSAAAL-----AANKKRPPPPPPPSRRN 321


>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 309

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 17/57 (29%), Positives = 23/57 (40%)
 Frame = +1

Query: 331 SPLPPRPAAQYDPAKSETYRALQEDGLPDAATELSAPVATKVFTAPTSKRPAPTPKP 501
           S LPP PA    P KS          +P    ++  P  ++   A TS RP+    P
Sbjct: 169 SSLPP-PAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPPLSQAPVANTSSRPSSFAPP 224


>SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1157

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 19/80 (23%), Positives = 35/80 (43%)
 Frame = +1

Query: 262 KQFNSPINLYSEQNIANSIRQQTSPLPPRPAAQYDPAKSETYRALQEDGLPDAATELSAP 441
           +QF+ PIN      +  ++ + TSP+    A       +E++ A  + G+        A 
Sbjct: 110 QQFSKPINESGTGTMGPAVGELTSPVMKNRAESIFSPVTESFEAFTQ-GMQTTPQRAGAG 168

Query: 442 VATKVFTAPTSKRPAPTPKP 501
           V+T   T+ T +R +    P
Sbjct: 169 VSTA--TSHTRRRSSAGTDP 186


>SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1210

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 1/37 (2%)
 Frame = -1

Query: 141  SSREHERSGAVRPVADN-FFFRMFRILVSVREEVGVE 34
            +S +  RS  ++P ADN   FR+ R ++S  EE+ ++
Sbjct: 980  ASTDESRSVLIKPKADNVILFRLKRFIMS-SEEINLD 1015


>SPBC651.09c |||RNA polymerase II associated Paf1 complex
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 560

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 16/83 (19%), Positives = 35/83 (42%)
 Frame = +1

Query: 256 LHKQFNSPINLYSEQNIANSIRQQTSPLPPRPAAQYDPAKSETYRALQEDGLPDAATELS 435
           L K+ +  +N   E ++ ++    ++        + +  ++ T   L E  + +    LS
Sbjct: 392 LVKEIDDQLNTLEELSMGSNQNSNSAMDQLAKVNERNRRRNHTEIRLAEQRMNEERRRLS 451

Query: 436 APVATKVFTAPTSKRPAPTPKPT 504
           A       +APTS     +P+P+
Sbjct: 452 AAATATPMSAPTSVLTGTSPQPS 474


>SPAC1142.08 |fhl1|SPAC8C9.01|fork head transcription factor Fhl1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 743

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 9/23 (39%), Positives = 14/23 (60%)
 Frame = +1

Query: 28  MSLNPNFFPNGYQDPKHPEEEVV 96
           ++LNP FF N   + + P E+ V
Sbjct: 461 LALNPEFFKNANGEQQAPNEQAV 483


>SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 387

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 10/32 (31%), Positives = 14/32 (43%)
 Frame = +1

Query: 289 YSEQNIANSIRQQTSPLPPRPAAQYDPAKSET 384
           Y+   + N      SPLPP  +  Y   + ET
Sbjct: 311 YTSNIVENPSEDNLSPLPPPASGPYSQEEEET 342


>SPAC25B8.19c ||SPAC683.01c|transcription factor, zf-fungal
           binuclear cluster type |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 522

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 21/80 (26%), Positives = 32/80 (40%), Gaps = 3/80 (3%)
 Frame = +1

Query: 271 NSP-INLYSEQNIANSIRQQTSPLPPR--PAAQYDPAKSETYRALQEDGLPDAATELSAP 441
           N+P IN  + +N   S    TS +     PA  Y   ++  +    +  +P  A +   P
Sbjct: 5   NTPSINRRNNENPPQSSLPTTSGIVYNMFPACPYPHVQNPAFHGSVD--VPQVAQKAFDP 62

Query: 442 VATKVFTAPTSKRPAPTPKP 501
            A  V  +    RP P P P
Sbjct: 63  QAATVSESANVSRPTPAPVP 82


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.310    0.128    0.385 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,875,387
Number of Sequences: 5004
Number of extensions: 38580
Number of successful extensions: 116
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.8 bits)

- SilkBase 1999-2023 -