BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30178 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 248 3e-67 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 139 3e-34 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 123 1e-29 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 86 3e-18 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 73 2e-14 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 49 2e-11 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 63 3e-11 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 54 1e-08 SPBC1683.03c |||membrane transporter|Schizosaccharomyces pombe|c... 27 1.3 SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 2.2 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 26 3.8 SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 25 5.1 SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 8.9 SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe... 25 8.9 SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 8.9 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 248 bits (608), Expect = 3e-67 Identities = 117/137 (85%), Positives = 126/137 (91%) Frame = +2 Query: 104 KILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITI 283 KIL ERGY+F+TTAEREIVRDIKEKLCYVALDFEQE+ TAA S+SLEKSYELPDGQVITI Sbjct: 191 KILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITI 250 Query: 284 GNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIA 463 GNERFR PEALFQPS LG+E+ GIHE YNSIMKCDVDIRKDLY N VMSGGTTMYPGIA Sbjct: 251 GNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIA 310 Query: 464 DRMQKEITALAPSTIKI 514 DRMQKEI ALAPS++K+ Sbjct: 311 DRMQKEIQALAPSSMKV 327 Score = 73.7 bits (173), Expect = 1e-14 Identities = 32/34 (94%), Positives = 34/34 (100%) Frame = +1 Query: 4 GVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 105 GV+HTVPIYEGYALPHAI+RLDLAGRDLTDYLMK Sbjct: 158 GVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMK 191 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 139 bits (336), Expect = 3e-34 Identities = 62/129 (48%), Positives = 90/129 (69%) Frame = +2 Query: 107 ILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIG 286 +L +GY+F TA+ E VR++KEKLCYV+ D E + + +T L ++Y LPDG+VI +G Sbjct: 192 LLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVG 251 Query: 287 NERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIAD 466 +ER+ CPE LFQP +G E G+ E ++++I DVDIRK LY V+SGG++MY G+ Sbjct: 252 SERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPS 311 Query: 467 RMQKEITAL 493 R++KEI L Sbjct: 312 RLEKEIKQL 320 Score = 46.0 bits (104), Expect = 3e-06 Identities = 21/40 (52%), Positives = 27/40 (67%) Frame = +1 Query: 4 GVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKDPHREG 123 GV+H VP+YE L H + RLD+AGRD T YL+ R+G Sbjct: 158 GVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKG 197 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 123 bits (297), Expect = 1e-29 Identities = 55/129 (42%), Positives = 82/129 (63%) Frame = +2 Query: 110 LTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGN 289 L + G+ ++AE+EIVR+IKEK CYVA DF E+ + +Y+LPD Q IT+G Sbjct: 198 LRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGT 257 Query: 290 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 469 E F PE LF P +G E+ G+H ++ SI+ D+D+R LY+N V+SGG+T+ G +R Sbjct: 258 ECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGER 317 Query: 470 MQKEITALA 496 E+ A++ Sbjct: 318 FISELRAIS 326 Score = 48.4 bits (110), Expect = 6e-07 Identities = 18/40 (45%), Positives = 30/40 (75%) Frame = +1 Query: 4 GVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKDPHREG 123 G++H+VPIY+G+++P AI + ++ GRD+TDYL + G Sbjct: 163 GLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSG 202 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 85.8 bits (203), Expect = 3e-18 Identities = 48/132 (36%), Positives = 70/132 (53%), Gaps = 9/132 (6%) Frame = +2 Query: 128 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCP 307 S+ +R ++ + KE + V + A AS+ K +E PDG G ERFR Sbjct: 243 SYEIFQKRRVIEEWKESVLDV---LDTPFDEAKASSRNPKPFEFPDGVTHKFGQERFRIS 299 Query: 308 EALFQPSFLGMESC---------GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGI 460 E LF PSF S G+HE VY SI+ CD ++R L N V++GGT++ PG+ Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGL 359 Query: 461 ADRMQKEITALA 496 ++R+Q E+ LA Sbjct: 360 SERLQAEVQRLA 371 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 73.3 bits (172), Expect = 2e-14 Identities = 49/157 (31%), Positives = 76/157 (48%), Gaps = 20/157 (12%) Frame = +2 Query: 104 KILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAAS--TSLEKSYELPD---- 265 ++++ R Y+ E IV +IKE +CYV+ +F+++M + LE Y LPD Sbjct: 199 EVISYRKYNMME--ETYIVNEIKESVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTG 256 Query: 266 --------------GQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIR 403 QV+ + NERF PE LF PS + + GI E V S+ +I+ Sbjct: 257 KHGYIVRDINQKIEQQVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQ 316 Query: 404 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKI 514 L N V GG +PG R+ E+ +LAP+ ++ Sbjct: 317 AMLLENVVTIGGNCKFPGFHKRLSSELRSLAPANWEV 353 Score = 27.1 bits (57), Expect = 1.7 Identities = 10/30 (33%), Positives = 19/30 (63%) Frame = +1 Query: 10 SHTVPIYEGYALPHAILRLDLAGRDLTDYL 99 +H +P+ + A+ R+D+ G+ LT+YL Sbjct: 168 THIIPVIDFSVQEQAVKRVDVGGKLLTNYL 197 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 48.8 bits (111), Expect(2) = 2e-11 Identities = 24/56 (42%), Positives = 35/56 (62%), Gaps = 1/56 (1%) Frame = +2 Query: 350 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAP-STIKI 514 GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P S +KI Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGSRLKI 381 Score = 34.3 bits (75), Expect(2) = 2e-11 Identities = 14/35 (40%), Positives = 21/35 (60%) Frame = +2 Query: 221 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQP 325 ++ +T E +E PDG + G ER++ PE LF P Sbjct: 254 SSETTIAESEFEFPDGSRMMFGAERYQIPEHLFVP 288 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 62.9 bits (146), Expect = 3e-11 Identities = 38/119 (31%), Positives = 58/119 (48%), Gaps = 5/119 (4%) Frame = +2 Query: 167 IKEKLCYVALDFEQEMATAAASTS--LEKSYELPDGQVITI--GNERFRCPEALFQPSFL 334 IKE+ CYV D +E + L+ + E G TI G ERF PE F P Sbjct: 240 IKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIA 299 Query: 335 GMESCG-IHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTI 508 + + E V N + +D+RK LY N V+SGG+T++ +R+Q+++ + I Sbjct: 300 SSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERI 358 Score = 36.7 bits (81), Expect = 0.002 Identities = 14/32 (43%), Positives = 23/32 (71%) Frame = +1 Query: 4 GVSHTVPIYEGYALPHAILRLDLAGRDLTDYL 99 GV+H +P+ EGY + +I + LAGRD+T ++ Sbjct: 189 GVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFV 220 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 54.0 bits (124), Expect = 1e-08 Identities = 21/70 (30%), Positives = 45/70 (64%) Frame = +2 Query: 290 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 469 E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 470 MQKEITALAP 499 +++E+T++ P Sbjct: 654 IKRELTSIMP 663 >SPBC1683.03c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 519 Score = 27.5 bits (58), Expect = 1.3 Identities = 12/30 (40%), Positives = 21/30 (70%), Gaps = 2/30 (6%) Frame = -1 Query: 120 LSVRIFHEVVGQ--VATSQVQTEDGVGQSV 37 +S+ +FHE+V T+Q+ T+ G+GQS+ Sbjct: 37 ISMSLFHEIVFVFIACTAQLMTQAGLGQSI 66 >SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 26.6 bits (56), Expect = 2.2 Identities = 9/16 (56%), Positives = 9/16 (56%) Frame = +2 Query: 26 STKVTLCPTPSSVWTW 73 STK CP PS W W Sbjct: 52 STKTATCPDPSWEWAW 67 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 25.8 bits (54), Expect = 3.8 Identities = 9/27 (33%), Positives = 18/27 (66%) Frame = +2 Query: 404 KDLYANTVMSGGTTMYPGIADRMQKEI 484 K+LY++ ++ GG +PG A +++ I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERI 553 >SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 25.4 bits (53), Expect = 5.1 Identities = 15/43 (34%), Positives = 20/43 (46%) Frame = +1 Query: 355 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 483 PR+ V R+R P P + R V PR RRQ +G+ Sbjct: 41 PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83 >SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 128 Score = 24.6 bits (51), Expect = 8.9 Identities = 13/43 (30%), Positives = 21/43 (48%), Gaps = 1/43 (2%) Frame = +2 Query: 182 CYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNE-RFRCP 307 CYV+ +E + + SL SY +PD + I + R+ P Sbjct: 39 CYVSRPLTKERSCSHPYISLGSSYGIPDAENIEYPRDARYHSP 81 >SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe|chr 3|||Manual Length = 301 Score = 24.6 bits (51), Expect = 8.9 Identities = 10/16 (62%), Positives = 11/16 (68%), Gaps = 1/16 (6%) Frame = +2 Query: 11 PTPYPSTKVTLC-PTP 55 P P P TKV +C PTP Sbjct: 256 PAPSPETKVLICGPTP 271 >SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 24.6 bits (51), Expect = 8.9 Identities = 16/36 (44%), Positives = 19/36 (52%) Frame = +2 Query: 167 IKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQV 274 IKEKL VA EQE A +SL+ S + G V Sbjct: 111 IKEKLRRVANITEQEQQCIPAESSLDLSIQAMKGVV 146 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,972,293 Number of Sequences: 5004 Number of extensions: 39368 Number of successful extensions: 168 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 153 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 165 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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