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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30178
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   248   3e-67
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...   139   3e-34
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...   123   1e-29
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...    86   3e-18
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po...    73   2e-14
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...    49   2e-11
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...    63   3e-11
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...    54   1e-08
SPBC1683.03c |||membrane transporter|Schizosaccharomyces pombe|c...    27   1.3  
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom...    27   2.2  
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb...    26   3.8  
SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc...    25   5.1  
SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|...    25   8.9  
SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe...    25   8.9  
SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual       25   8.9  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  248 bits (608), Expect = 3e-67
 Identities = 117/137 (85%), Positives = 126/137 (91%)
 Frame = +2

Query: 104 KILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITI 283
           KIL ERGY+F+TTAEREIVRDIKEKLCYVALDFEQE+ TAA S+SLEKSYELPDGQVITI
Sbjct: 191 KILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITI 250

Query: 284 GNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIA 463
           GNERFR PEALFQPS LG+E+ GIHE  YNSIMKCDVDIRKDLY N VMSGGTTMYPGIA
Sbjct: 251 GNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIA 310

Query: 464 DRMQKEITALAPSTIKI 514
           DRMQKEI ALAPS++K+
Sbjct: 311 DRMQKEIQALAPSSMKV 327



 Score = 73.7 bits (173), Expect = 1e-14
 Identities = 32/34 (94%), Positives = 34/34 (100%)
 Frame = +1

Query: 4   GVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 105
           GV+HTVPIYEGYALPHAI+RLDLAGRDLTDYLMK
Sbjct: 158 GVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMK 191


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score =  139 bits (336), Expect = 3e-34
 Identities = 62/129 (48%), Positives = 90/129 (69%)
 Frame = +2

Query: 107 ILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIG 286
           +L  +GY+F  TA+ E VR++KEKLCYV+ D E +   +  +T L ++Y LPDG+VI +G
Sbjct: 192 LLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVG 251

Query: 287 NERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIAD 466
           +ER+ CPE LFQP  +G E  G+ E ++++I   DVDIRK LY   V+SGG++MY G+  
Sbjct: 252 SERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPS 311

Query: 467 RMQKEITAL 493
           R++KEI  L
Sbjct: 312 RLEKEIKQL 320



 Score = 46.0 bits (104), Expect = 3e-06
 Identities = 21/40 (52%), Positives = 27/40 (67%)
 Frame = +1

Query: 4   GVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKDPHREG 123
           GV+H VP+YE   L H + RLD+AGRD T YL+    R+G
Sbjct: 158 GVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKG 197


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score =  123 bits (297), Expect = 1e-29
 Identities = 55/129 (42%), Positives = 82/129 (63%)
 Frame = +2

Query: 110 LTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGN 289
           L + G+   ++AE+EIVR+IKEK CYVA DF  E+ +         +Y+LPD Q IT+G 
Sbjct: 198 LRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGT 257

Query: 290 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 469
           E F  PE LF P  +G E+ G+H  ++ SI+  D+D+R  LY+N V+SGG+T+  G  +R
Sbjct: 258 ECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGER 317

Query: 470 MQKEITALA 496
              E+ A++
Sbjct: 318 FISELRAIS 326



 Score = 48.4 bits (110), Expect = 6e-07
 Identities = 18/40 (45%), Positives = 30/40 (75%)
 Frame = +1

Query: 4   GVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKDPHREG 123
           G++H+VPIY+G+++P AI + ++ GRD+TDYL     + G
Sbjct: 163 GLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSG 202


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score = 85.8 bits (203), Expect = 3e-18
 Identities = 48/132 (36%), Positives = 70/132 (53%), Gaps = 9/132 (6%)
 Frame = +2

Query: 128 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCP 307
           S+    +R ++ + KE +  V    +     A AS+   K +E PDG     G ERFR  
Sbjct: 243 SYEIFQKRRVIEEWKESVLDV---LDTPFDEAKASSRNPKPFEFPDGVTHKFGQERFRIS 299

Query: 308 EALFQPSFLGMESC---------GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGI 460
           E LF PSF    S          G+HE VY SI+ CD ++R  L  N V++GGT++ PG+
Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGL 359

Query: 461 ADRMQKEITALA 496
           ++R+Q E+  LA
Sbjct: 360 SERLQAEVQRLA 371


>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 401

 Score = 73.3 bits (172), Expect = 2e-14
 Identities = 49/157 (31%), Positives = 76/157 (48%), Gaps = 20/157 (12%)
 Frame = +2

Query: 104 KILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAAS--TSLEKSYELPD---- 265
           ++++ R Y+     E  IV +IKE +CYV+ +F+++M        + LE  Y LPD    
Sbjct: 199 EVISYRKYNMME--ETYIVNEIKESVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTG 256

Query: 266 --------------GQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIR 403
                          QV+ + NERF  PE LF PS + +   GI E V  S+     +I+
Sbjct: 257 KHGYIVRDINQKIEQQVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQ 316

Query: 404 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKI 514
             L  N V  GG   +PG   R+  E+ +LAP+  ++
Sbjct: 317 AMLLENVVTIGGNCKFPGFHKRLSSELRSLAPANWEV 353



 Score = 27.1 bits (57), Expect = 1.7
 Identities = 10/30 (33%), Positives = 19/30 (63%)
 Frame = +1

Query: 10  SHTVPIYEGYALPHAILRLDLAGRDLTDYL 99
           +H +P+ +      A+ R+D+ G+ LT+YL
Sbjct: 168 THIIPVIDFSVQEQAVKRVDVGGKLLTNYL 197


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 48.8 bits (111), Expect(2) = 2e-11
 Identities = 24/56 (42%), Positives = 35/56 (62%), Gaps = 1/56 (1%)
 Frame = +2

Query: 350 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAP-STIKI 514
           GI +   N I +CDVDIR  L  N ++ GGT++  G + R+Q E++ L P S +KI
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGSRLKI 381



 Score = 34.3 bits (75), Expect(2) = 2e-11
 Identities = 14/35 (40%), Positives = 21/35 (60%)
 Frame = +2

Query: 221 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQP 325
           ++ +T  E  +E PDG  +  G ER++ PE LF P
Sbjct: 254 SSETTIAESEFEFPDGSRMMFGAERYQIPEHLFVP 288


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score = 62.9 bits (146), Expect = 3e-11
 Identities = 38/119 (31%), Positives = 58/119 (48%), Gaps = 5/119 (4%)
 Frame = +2

Query: 167 IKEKLCYVALDFEQEMATAAASTS--LEKSYELPDGQVITI--GNERFRCPEALFQPSFL 334
           IKE+ CYV  D  +E +         L+ + E   G   TI  G ERF  PE  F P   
Sbjct: 240 IKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIA 299

Query: 335 GMESCG-IHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTI 508
             +    + E V N +    +D+RK LY N V+SGG+T++    +R+Q+++  +    I
Sbjct: 300 SSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERI 358



 Score = 36.7 bits (81), Expect = 0.002
 Identities = 14/32 (43%), Positives = 23/32 (71%)
 Frame = +1

Query: 4   GVSHTVPIYEGYALPHAILRLDLAGRDLTDYL 99
           GV+H +P+ EGY +  +I  + LAGRD+T ++
Sbjct: 189 GVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFV 220


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score = 54.0 bits (124), Expect = 1e-08
 Identities = 21/70 (30%), Positives = 45/70 (64%)
 Frame = +2

Query: 290 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 469
           E+ R PE +F PS +G++  GI E + + + +  ++ ++ L +N +++GG    PG+  R
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653

Query: 470 MQKEITALAP 499
           +++E+T++ P
Sbjct: 654 IKRELTSIMP 663


>SPBC1683.03c |||membrane transporter|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 519

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 12/30 (40%), Positives = 21/30 (70%), Gaps = 2/30 (6%)
 Frame = -1

Query: 120 LSVRIFHEVVGQ--VATSQVQTEDGVGQSV 37
           +S+ +FHE+V      T+Q+ T+ G+GQS+
Sbjct: 37  ISMSLFHEIVFVFIACTAQLMTQAGLGQSI 66


>SPAC1296.04 |mug65||spore wall assembly protein
          |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 26.6 bits (56), Expect = 2.2
 Identities = 9/16 (56%), Positives = 9/16 (56%)
 Frame = +2

Query: 26 STKVTLCPTPSSVWTW 73
          STK   CP PS  W W
Sbjct: 52 STKTATCPDPSWEWAW 67


>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 620

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 9/27 (33%), Positives = 18/27 (66%)
 Frame = +2

Query: 404 KDLYANTVMSGGTTMYPGIADRMQKEI 484
           K+LY++ ++ GG   +PG A  +++ I
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERI 553


>SPBC16A3.08c |||nuclear telomere cap complex subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 284

 Score = 25.4 bits (53), Expect = 5.1
 Identities = 15/43 (34%), Positives = 20/43 (46%)
 Frame = +1

Query: 355 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 483
           PR+ V      R+R P  P  + R V      PR RRQ  +G+
Sbjct: 41  PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83


>SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 128

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 13/43 (30%), Positives = 21/43 (48%), Gaps = 1/43 (2%)
 Frame = +2

Query: 182 CYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNE-RFRCP 307
           CYV+    +E + +    SL  SY +PD + I    + R+  P
Sbjct: 39  CYVSRPLTKERSCSHPYISLGSSYGIPDAENIEYPRDARYHSP 81


>SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 301

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 10/16 (62%), Positives = 11/16 (68%), Gaps = 1/16 (6%)
 Frame = +2

Query: 11  PTPYPSTKVTLC-PTP 55
           P P P TKV +C PTP
Sbjct: 256 PAPSPETKVLICGPTP 271


>SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 16/36 (44%), Positives = 19/36 (52%)
 Frame = +2

Query: 167 IKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQV 274
           IKEKL  VA   EQE     A +SL+ S +   G V
Sbjct: 111 IKEKLRRVANITEQEQQCIPAESSLDLSIQAMKGVV 146


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,972,293
Number of Sequences: 5004
Number of extensions: 39368
Number of successful extensions: 168
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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