SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30166
         (516 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g22060.1 68414.m02759 expressed protein                             31   0.46 
At5g08110.1 68418.m00946 DEAD/DEAH box helicase, putative severa...    29   1.4  
At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote...    29   1.4  
At3g18370.1 68416.m02336 C2 domain-containing protein contains P...    29   2.5  
At1g16540.1 68414.m01981 molybdenum cofactor sulfurase (LOS5) (A...    29   2.5  
At5g57230.1 68418.m07150 expressed protein                             28   4.3  
At5g55540.1 68418.m06919 expressed protein                             28   4.3  
At5g12970.1 68418.m01487 C2 domain-containing protein contains I...    28   4.3  
At5g25060.1 68418.m02970 RNA recognition motif (RRM)-containing ...    27   5.7  
At4g34920.1 68417.m04951 1-phosphatidylinositol phosphodiesteras...    27   5.7  
At5g47420.1 68418.m05843 expressed protein contains Pfam domain,...    27   7.5  
At3g19040.1 68416.m02418 ubiquitin family protein / DNA-binding ...    27   7.5  
At3g02980.1 68416.m00293 GCN5-related N-acetyltransferase (GNAT)...    27   7.5  
At1g54870.1 68414.m06265 short-chain dehydrogenase/reductase (SD...    27   7.5  
At5g24440.1 68418.m02880 RNA-binding protein, putative                 27   9.9  
At4g34930.1 68417.m04952 1-phosphatidylinositol phosphodiesteras...    27   9.9  
At4g17420.1 68417.m02608 expressed protein contains Pfam domain,...    27   9.9  
At1g48405.1 68414.m05407 hypothetical protein                          27   9.9  

>At1g22060.1 68414.m02759 expressed protein
          Length = 1999

 Score = 31.1 bits (67), Expect = 0.46
 Identities = 13/37 (35%), Positives = 22/37 (59%)
 Frame = +2

Query: 125  ESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINE 235
            E MLDT   +S++  E++ + +   +LSLKF  +  E
Sbjct: 1932 EEMLDTKGRYSSMETELREMHDRYSQLSLKFAEVEGE 1968


>At5g08110.1 68418.m00946 DEAD/DEAH box helicase, putative several
           putative ATP-dependent helicases
          Length = 1058

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 19/90 (21%), Positives = 44/90 (48%), Gaps = 1/90 (1%)
 Frame = +2

Query: 242 VEGDKYQISIHLPGYEQKDINVKAKNGVLMVQANSAFNHYLKIQNLPWDVNSEGSWVYEK 421
           + G+   + +   G   +D+    K+      ++S F+ Y+K + L  D       + + 
Sbjct: 20  INGESTTVQVSSDG-TIRDLKKALKSCFPPASSSSNFHLYIKGEKLKLDTRVAAITINDG 78

Query: 422 DVLKITFPLKQKQPEDSKRP-VAEPTETTS 508
           D+L + FP K+K+   + +P +++P+  +S
Sbjct: 79  DILGL-FPFKKKELRQTPKPDLSKPSSLSS 107


>At1g52560.1 68414.m05933 26.5 kDa class I small heat shock
           protein-like (HSP26.5-P) contains Pfam profile: PF00011
           Hsp20/alpha crystallin family; identified in Scharf,
           K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
          Length = 232

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 12/79 (15%), Positives = 40/79 (50%)
 Frame = +2

Query: 107 FSPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGRVEGDKYQISIHLPGY 286
           F+P + E    T  + + L    ++++ +    ++    ++ + + + D Y++   +PG 
Sbjct: 88  FTPTLNEFFPPT--IGNTLIQATENMNRIFDNFNVNPFQLMGQVKEQDDCYKLRYEVPGL 145

Query: 287 EQKDINVKAKNGVLMVQAN 343
            ++D+ +   +G+L ++ +
Sbjct: 146 TKEDVKITVNDGILTIKGD 164


>At3g18370.1 68416.m02336 C2 domain-containing protein contains Pfam
           profile: PF00168 C2 domain
          Length = 815

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 11/28 (39%), Positives = 20/28 (71%)
 Frame = +2

Query: 167 NEMQHLDNMMKELSLKFPSIINEGRVEG 250
           N+   L N MK++ +KF ++I++G +EG
Sbjct: 735 NKALLLSNQMKQVMIKFQNLIDDGDLEG 762


>At1g16540.1 68414.m01981 molybdenum cofactor sulfurase (LOS5)
           (ABA3) identical to molybdenum cofactor sulfurase
           (LOS5/ABA3) [Arabidopsis thaliana] GI:15407262;
           supporting cDNA gi|15407261|gb|AY034895.1|
          Length = 819

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 15/48 (31%), Positives = 23/48 (47%)
 Frame = -1

Query: 513 FVEVVSVGSATGLLLSSGCFCFSGKVIFNTSFS*TQLPSEFTSQGRFC 370
           ++EV  + S +G+ L +GCFC  G        S ++L S     G  C
Sbjct: 410 YLEVEKLASLSGIQLRTGCFCNPGACAKYLELSHSELRSN-VEAGHIC 456


>At5g57230.1 68418.m07150 expressed protein 
          Length = 160

 Score = 27.9 bits (59), Expect = 4.3
 Identities = 14/32 (43%), Positives = 19/32 (59%)
 Frame = +2

Query: 164 ANEMQHLDNMMKELSLKFPSIINEGRVEGDKY 259
           +N  QHLD M++E + +F   I   RVE  KY
Sbjct: 60  SNYTQHLDRMLEEAAAEFYPNIKFMRVECPKY 91


>At5g55540.1 68418.m06919 expressed protein
          Length = 1380

 Score = 27.9 bits (59), Expect = 4.3
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = +2

Query: 185 DNMMKELSLKFPSIINEGRVEGDKYQISIH 274
           D    + SL  PSI+ EGR +  K+QI+ H
Sbjct: 866 DPSSPDSSLLVPSILEEGRGKTQKWQINTH 895


>At5g12970.1 68418.m01487 C2 domain-containing protein contains
           INTERPRO:IPR000008 C2 domain
          Length = 769

 Score = 27.9 bits (59), Expect = 4.3
 Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
 Frame = +1

Query: 217 PQHYKRRTR-GRRQVSDIYSPAWLRTERHQRESEKWSADGA 336
           PQ Y+   R GR+   ++    W+ T+  +  S+ W +D A
Sbjct: 140 PQWYRLEDRHGRKVKGELMLAVWMGTQADEAFSDAWHSDAA 180


>At5g25060.1 68418.m02970 RNA recognition motif (RRM)-containing
           protein KIAA0332 - Homo sapiens, EMBL:AB002330
          Length = 946

 Score = 27.5 bits (58), Expect = 5.7
 Identities = 9/26 (34%), Positives = 15/26 (57%)
 Frame = +2

Query: 143 HSLWSNLANEMQHLDNMMKELSLKFP 220
           HS W  + +E +H+ N   E+ +K P
Sbjct: 686 HSTWEEVKSEREHIKNSYAEVEMKEP 711


>At4g34920.1 68417.m04951 1-phosphatidylinositol
           phosphodiesterase-related contains weak similarity to
           1-phosphatidylinositol phosphodiesterase precursor (EC
           3.1.4.10) (Phosphatidylinositol-specific phospholipase
           C) (PI-PLC). (Swiss-Prot:P34024) [Listeria
           monocytogenes]
          Length = 318

 Score = 27.5 bits (58), Expect = 5.7
 Identities = 10/36 (27%), Positives = 19/36 (52%)
 Frame = +2

Query: 110 SPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKF 217
           S Y++++ +DT   W+   + ++HL       S KF
Sbjct: 209 SDYLKDNWIDTDLPWTKFQSNLKHLSEQQPTSSRKF 244


>At5g47420.1 68418.m05843 expressed protein contains Pfam domain,
           PF01987: Protein of unknown function
          Length = 282

 Score = 27.1 bits (57), Expect = 7.5
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = +2

Query: 104 PFSPYVRESMLDTHSLWSNLANEMQHLDNMMK 199
           PF PYV +S  DT + +  L  E Q +  M+K
Sbjct: 9   PFQPYVYQSQQDTITPFQILGGESQVVQIMLK 40


>At3g19040.1 68416.m02418 ubiquitin family protein / DNA-binding
            bromodomain-containing protein low similarity to
            SP|P51123 Transcription initiation factor TFIID 230 kDa
            subunit {Drosophila melanogaster}; contains Pfam
            profiles: PF00439 bromodomain, PF00240: Ubiquitin family
          Length = 1700

 Score = 27.1 bits (57), Expect = 7.5
 Identities = 12/41 (29%), Positives = 21/41 (51%)
 Frame = +1

Query: 166  QRNATLGQHDEGAVVEVPQHYKRRTRGRRQVSDIYSPAWLR 288
            +R   +  HD G++ E PQ   R+T+   +++D      LR
Sbjct: 1410 KRLKEITDHDSGSLEETPQFESRKTKRMAELADFQRQQRLR 1450


>At3g02980.1 68416.m00293 GCN5-related N-acetyltransferase (GNAT)
           family protein contains Pfam profile: PF00583
           acetyltransferase (GNAT) family
          Length = 247

 Score = 27.1 bits (57), Expect = 7.5
 Identities = 13/30 (43%), Positives = 17/30 (56%)
 Frame = -3

Query: 373 LYFQVMIKSTVSLHHQHSIFRFHVDVFLFV 284
           LY ++M +    LH  + I R H D FLFV
Sbjct: 165 LYKRLMFRCVRRLHGFYLINRHHFDAFLFV 194


>At1g54870.1 68414.m06265 short-chain dehydrogenase/reductase (SDR)
           family protein C-terminal similar to dormancy related
           protein GI:1220178 from [Trollius ledebourii]
          Length = 288

 Score = 27.1 bits (57), Expect = 7.5
 Identities = 12/28 (42%), Positives = 17/28 (60%)
 Frame = +2

Query: 416 EKDVLKITFPLKQKQPEDSKRPVAEPTE 499
           EKD  +    LK+ +  DSK P+A PT+
Sbjct: 74  EKDAQETLQMLKEVKTSDSKEPIAIPTD 101


>At5g24440.1 68418.m02880 RNA-binding protein, putative
          Length = 320

 Score = 26.6 bits (56), Expect = 9.9
 Identities = 12/41 (29%), Positives = 21/41 (51%)
 Frame = +2

Query: 158 NLANEMQHLDNMMKELSLKFPSIINEGRVEGDKYQISIHLP 280
           NL +E+ HL+ M KE    F +  +     G+++  + H P
Sbjct: 58  NLKSEISHLNPMAKEFVPSFLAQTHHSEFWGNRFWFTNHFP 98


>At4g34930.1 68417.m04952 1-phosphatidylinositol
           phosphodiesterase-related contains weak similarity to
           1-phosphatidylinositol phosphodiesterase precursor (EC
           4.6.1.13) (Phosphatidylinositol diacylglycerol-lyase)
           (Phosphatidylinositol- specific phospholipase C)
           (PI-PLC). (Swiss-Prot:P34024) [Listeria monocytogenes]
          Length = 391

 Score = 26.6 bits (56), Expect = 9.9
 Identities = 10/36 (27%), Positives = 19/36 (52%)
 Frame = +2

Query: 110 SPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKF 217
           S Y++++ +DT   W+   + ++HL       S KF
Sbjct: 276 SDYLKDNWIDTDLPWTKFQSNLKHLSEQQPISSRKF 311


>At4g17420.1 68417.m02608 expressed protein contains Pfam domain,
           PF01987: Protein of unknown function
          Length = 285

 Score = 26.6 bits (56), Expect = 9.9
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = +2

Query: 104 PFSPYVRESMLDTHSLWSNLANEMQHLDNMMK 199
           PF PYV +S  DT + +  L  E Q +  M+K
Sbjct: 9   PFQPYVYQSQEDTITPFQILGGEAQVVQIMLK 40


>At1g48405.1 68414.m05407 hypothetical protein
          Length = 297

 Score = 26.6 bits (56), Expect = 9.9
 Identities = 18/71 (25%), Positives = 34/71 (47%)
 Frame = +2

Query: 293 KDINVKAKNGVLMVQANSAFNHYLKIQNLPWDVNSEGSWVYEKDVLKITFPLKQKQPEDS 472
           K+  VK    VL ++ +  F+    +QN  +++ S    VYE +  +  F L+  + +  
Sbjct: 193 KNEAVKLARCVLKMR-DEHFHKMCHLQNQIYELQSSREPVYENESSQSCFGLENSKKKSK 251

Query: 473 KRPVAEPTETT 505
           KR ++E    T
Sbjct: 252 KRKMSETRSET 262


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,244,027
Number of Sequences: 28952
Number of extensions: 228410
Number of successful extensions: 804
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 775
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 801
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -