Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30162
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 29 1.9
At5g28210.1 68418.m03416 mRNA capping enzyme family protein simi... 29 2.5
At4g39980.1 68417.m05662 2-dehydro-3-deoxyphosphoheptonate aldol... 29 2.5
At1g74510.2 68414.m08632 kelch repeat-containing F-box family pr... 29 2.5
At1g74510.1 68414.m08631 kelch repeat-containing F-box family pr... 29 2.5
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 3.2
At1g05790.1 68414.m00605 lipase class 3 family protein contains ... 28 3.2
At1g80870.1 68414.m09489 protein kinase family protein contains ... 28 4.3
At1g72110.1 68414.m08335 expressed protein 27 5.7
At1g09070.1 68414.m01012 C2 domain-containing protein / src2-lik... 27 5.7
At5g49360.1 68418.m06108 glycosyl hydrolase family 3 protein 27 7.5
At5g56970.1 68418.m07111 FAD-binding domain-containing protein /... 27 9.9
At1g53450.2 68414.m06059 expressed protein 27 9.9
At1g53450.1 68414.m06058 expressed protein 27 9.9
At1g20410.1 68414.m02545 expressed protein 27 9.9
>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
subunit (RPB205) (RPII) (RPB1) nearly identical to
P|P18616 DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) {Arabidopsis thaliana}
Length = 1840
Score = 29.1 bits (62), Expect = 1.9
Identities = 21/66 (31%), Positives = 27/66 (40%), Gaps = 3/66 (4%)
Frame = +2
Query: 68 TNNGIAAQEQGVPRNLGGNPPAVPVVAQGS--FSWTSPEGVPISVNYVADENGYQPTGNA 241
T+ G + G G P P + S +S TSP P S +Y Y PT +
Sbjct: 1555 TSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPTSPSYSPTSPSYSPTSPS 1614
Query: 242 I-PTSP 256
PTSP
Sbjct: 1615 YSPTSP 1620
Score = 29.1 bits (62), Expect = 1.9
Identities = 21/57 (36%), Positives = 26/57 (45%), Gaps = 4/57 (7%)
Frame = +2
Query: 155 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSPPVPEQIAR---ALAYIAKNIPL 313
S+S TSP P S Y Y PT + PTSP Q A+ ++AY N L
Sbjct: 1691 SYSPTSPAYSPTSPGYSPTSPSYSPTSPSYGPTSPSYNPQSAKYSPSIAYSPSNARL 1747
Score = 28.7 bits (61), Expect = 2.5
Identities = 22/72 (30%), Positives = 27/72 (37%), Gaps = 1/72 (1%)
Frame = +2
Query: 44 GSYTYFYETNNGIAAQEQGVPRNLGGNPPAVPVVAQGSFSWTSPEGVPISVNYVADENGY 223
G + ++ G + G G P P +S TSP P S Y GY
Sbjct: 1533 GGMAFSPSSSPGYSPSSPGYSPTSPGYSPTSP-----GYSPTSPGYSPTSPTYSPSSPGY 1587
Query: 224 QPTGNAI-PTSP 256
PT A PTSP
Sbjct: 1588 SPTSPAYSPTSP 1599
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 155 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 256
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1614 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1648
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 155 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 256
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1621 SYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSP 1655
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 155 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 256
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1670 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1704
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 155 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 256
S+S TSP P S Y GY PT + PTSP
Sbjct: 1684 SYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTSP 1718
Score = 27.1 bits (57), Expect = 7.5
Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 155 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 256
S+S TSP P S +Y Y PT + PTSP
Sbjct: 1600 SYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1634
Score = 27.1 bits (57), Expect = 7.5
Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 155 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 256
S+S TSP P S +Y Y PT + PTSP
Sbjct: 1607 SYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1641
Score = 27.1 bits (57), Expect = 7.5
Identities = 16/35 (45%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
Frame = +2
Query: 155 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 256
S+S TSP P S Y Y PT A PTSP
Sbjct: 1628 SYSPTSPSYSPTSPAYSPTSPAYSPTSPAYSPTSP 1662
Score = 27.1 bits (57), Expect = 7.5
Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 155 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 256
S+S TSP P S +Y Y PT + PTSP
Sbjct: 1663 SYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1697
Score = 26.6 bits (56), Expect = 9.9
Identities = 20/66 (30%), Positives = 31/66 (46%), Gaps = 7/66 (10%)
Frame = +3
Query: 138 PSLHRAHSPGHLLKVFP------SASITSPTRTDTSPLVMLSPLPHQCLSRSLVL-LPTS 296
PSLH+ GH +++ P + S+TSP D M +P +R+ VL L
Sbjct: 463 PSLHKMSIMGHRIRIMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSFETRAEVLELMMV 522
Query: 297 PRTFLS 314
P+ +S
Sbjct: 523 PKCIVS 528
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/35 (42%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
Frame = +2
Query: 155 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 256
S+S TSP P S +Y Y PT PTSP
Sbjct: 1677 SYSPTSPSYSPTSPSYSPTSPAYSPTSPGYSPTSP 1711
>At5g28210.1 68418.m03416 mRNA capping enzyme family protein similar
to mRNA capping enzyme [Xenopus laevis] GI:7239232;
contains Pfam profiles PF01331: mRNA capping enzyme
catalytic domain, PF03919: mRNA capping enzyme
C-terminal domain
Length = 625
Score = 28.7 bits (61), Expect = 2.5
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = -2
Query: 185 EHLQEMSRRMSPVQRREQQGGFHQGYVELPVLGQ 84
E+ +E S+R+ P R+Q+ QG+++ P GQ
Sbjct: 27 ENREEHSKRLRPTSFRDQKVTIPQGWLDCPRFGQ 60
>At4g39980.1 68417.m05662 2-dehydro-3-deoxyphosphoheptonate aldolase
1 / 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
1 / DAHP synthetase 1 (DHS1) nearly identical to
SP|P29965
Length = 525
Score = 28.7 bits (61), Expect = 2.5
Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 1/76 (1%)
Frame = +2
Query: 116 GGNPPAVPVVAQGSFSWTSPEGV-PISVNYVADENGYQPTGNAIPTSPPVPEQIARALAY 292
GG+ P Q SF++ P SVN V + +P NA+ V + AL +
Sbjct: 16 GGDLSHRPSNRQSSFTFHPAVNTKPKSVNLVTAVHAAEPARNAVSVKESVASSSSGALKW 75
Query: 293 IAKNIPLKK*AINLTD 340
++ LKK A+ L D
Sbjct: 76 TPESWKLKK-ALQLPD 90
>At1g74510.2 68414.m08632 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 28.7 bits (61), Expect = 2.5
Identities = 18/53 (33%), Positives = 24/53 (45%), Gaps = 2/53 (3%)
Frame = +2
Query: 2 GAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPP--AVPVVAQG 154
G E + FG EI Y Y TN + + VPR L G+ + V+A G
Sbjct: 191 GTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243
>At1g74510.1 68414.m08631 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 28.7 bits (61), Expect = 2.5
Identities = 18/53 (33%), Positives = 24/53 (45%), Gaps = 2/53 (3%)
Frame = +2
Query: 2 GAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPP--AVPVVAQG 154
G E + FG EI Y Y TN + + VPR L G+ + V+A G
Sbjct: 191 GTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/37 (43%), Positives = 24/37 (64%)
Frame = +3
Query: 108 VTLVETPLLFPSLHRAHSPGHLLKVFPSASITSPTRT 218
++LV TPLLF + A + G LL+ FPS + +SP +
Sbjct: 518 LSLVTTPLLFKLIPSAMNLGVLLRWFPSEN-SSPNES 553
>At1g05790.1 68414.m00605 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 687
Score = 28.3 bits (60), Expect = 3.2
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = -3
Query: 250 SGDSITSGLVSVLVGDVIDADGNTFR 173
SG+S +SG +S L+GD + DG + R
Sbjct: 398 SGESESSGFLSSLIGDGCECDGYSIR 423
>At1g80870.1 68414.m09489 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 692
Score = 27.9 bits (59), Expect = 4.3
Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 4/46 (8%)
Frame = -3
Query: 319 LLERNVLGDVGKSTSDLLRHWW----GSGDSITSGLVSVLVGDVID 194
LL N + D+GK + R WW GSG+ + V +G ID
Sbjct: 330 LLSPNSVLDLGKGSKQWGRDWWWKQEGSGELCSKDYVREWIGSQID 375
>At1g72110.1 68414.m08335 expressed protein
Length = 479
Score = 27.5 bits (58), Expect = 5.7
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = +2
Query: 2 GAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNL 115
G+ T+ F N + PD ++F IAA GVP+ L
Sbjct: 389 GSTTMIFSNVVGPDEEISFFGHRIAYIAASTFGVPQAL 426
>At1g09070.1 68414.m01012 C2 domain-containing protein / src2-like
protein, putative similar to cold-regulated gene SRC2
[Glycine max] GI:2055230; contains Pfam profile PF00168:
C2 domain; identical to cDNA src2-like protein
GI:3426059
Length = 324
Score = 27.5 bits (58), Expect = 5.7
Identities = 17/55 (30%), Positives = 21/55 (38%), Gaps = 6/55 (10%)
Frame = +2
Query: 89 QEQGVPRNLGGNPPAVPVVAQGSFSWTSPE------GVPISVNYVADENGYQPTG 235
Q+ G P GG PP QG + P+ G P Y + GY P G
Sbjct: 204 QQAGYPAGTGGYPPPGAYPQQGGYPGYPPQQQGGYPGYPPQGPYGYPQQGYPPQG 258
>At5g49360.1 68418.m06108 glycosyl hydrolase family 3 protein
Length = 774
Score = 27.1 bits (57), Expect = 7.5
Identities = 17/62 (27%), Positives = 28/62 (45%)
Frame = +2
Query: 107 RNLGGNPPAVPVVAQGSFSWTSPEGVPISVNYVADENGYQPTGNAIPTSPPVPEQIARAL 286
RNL N AVP + G + W S +++ ++D G A P + P+ I A
Sbjct: 74 RNLVNNAAAVPRLGIGGYEWWSE-----ALHGISDVGPGAKFGGAFPGATSFPQVITTAA 128
Query: 287 AY 292
++
Sbjct: 129 SF 130
>At5g56970.1 68418.m07111 FAD-binding domain-containing protein /
cytokinin oxidase family protein similar to cytokinin
oxidase from Zea mays [gi:3882018] [gi:3341978]
Length = 523
Score = 26.6 bits (56), Expect = 9.9
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +3
Query: 165 GHLLKVFPSASITSPTRTDTSPLVMLS 245
GH+ K+FPSA + + D + L+ LS
Sbjct: 63 GHVTKIFPSAVLIPSSVEDITDLIKLS 89
>At1g53450.2 68414.m06059 expressed protein
Length = 453
Score = 26.6 bits (56), Expect = 9.9
Identities = 19/48 (39%), Positives = 24/48 (50%)
Frame = +3
Query: 147 HRAHSPGHLLKVFPSASITSPTRTDTSPLVMLSPLPHQCLSRSLVLLP 290
H + G L +V S S SP R D+S L +L P L S +LLP
Sbjct: 185 HSLAARGDLWRVEASTS-NSPVRDDSSSLFLLQLGPLLFLRDSTLLLP 231
>At1g53450.1 68414.m06058 expressed protein
Length = 453
Score = 26.6 bits (56), Expect = 9.9
Identities = 19/48 (39%), Positives = 24/48 (50%)
Frame = +3
Query: 147 HRAHSPGHLLKVFPSASITSPTRTDTSPLVMLSPLPHQCLSRSLVLLP 290
H + G L +V S S SP R D+S L +L P L S +LLP
Sbjct: 185 HSLAARGDLWRVEASTS-NSPVRDDSSSLFLLQLGPLLFLRDSTLLLP 231
>At1g20410.1 68414.m02545 expressed protein
Length = 504
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 2/39 (5%)
Frame = -3
Query: 340 VCE--IYRLLLERNVLGDVGKSTSDLLRHWWGSGDSITS 230
VCE I+RL+ D+ ++ LR W SGD TS
Sbjct: 50 VCERCIFRLVSVEAFDSDISSVSTSTLRSWLKSGDDETS 88
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,675,612
Number of Sequences: 28952
Number of extensions: 268319
Number of successful extensions: 814
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 762
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 800
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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