BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30139 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC19C2.05 |ran1|pat1|serine/threonine protein kinase Ran1|Schi... 29 0.31 SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 26 2.9 SPBC29B5.04c |||conserved fungal protein|Schizosaccharomyces pom... 25 5.1 SPAC1565.02c |||GTPase activating protein|Schizosaccharomyces po... 25 5.1 SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-... 25 6.7 SPBC543.06c |dbp8||ATP-dependent RNA helicase Dbp8 |Schizosaccha... 25 6.7 SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||... 25 8.9 SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit Nse6|Schizosa... 25 8.9 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 8.9 SPCC584.15c |||arrestin/PY protein 2|Schizosaccharomyces pombe|c... 25 8.9 >SPBC19C2.05 |ran1|pat1|serine/threonine protein kinase Ran1|Schizosaccharomyces pombe|chr 2|||Manual Length = 470 Score = 29.5 bits (63), Expect = 0.31 Identities = 19/46 (41%), Positives = 24/46 (52%), Gaps = 2/46 (4%) Frame = -2 Query: 416 HNPVLRLHYLNLSREFRSLARGGGDL--RNRFTLSMVSSLVSEVRN 285 HNP L L +SRE SL D + R TL +S+LVS +N Sbjct: 255 HNPSTLLSILPISRELNSLLNRIFDRNPKTRITLPELSTLVSNCKN 300 >SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2670 Score = 26.2 bits (55), Expect = 2.9 Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 1/46 (2%) Frame = -2 Query: 314 VSSLVSEVRNGFRFAVSVRV-TVASTHSNNNVRLVVVVESLLQLTS 180 V SL ++ + R A V TVA +N+ RL VV++SLL + S Sbjct: 1288 VDSLPTDANDRLREATVVLFGTVAQHLPSNDPRLAVVMDSLLSVLS 1333 >SPBC29B5.04c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 605 Score = 25.4 bits (53), Expect = 5.1 Identities = 12/37 (32%), Positives = 17/37 (45%) Frame = +3 Query: 177 ETGELKEALDDDNKPHVIVAVRGSYSYTNTDGKPETI 287 ETG E D++ V+V + + DGKP I Sbjct: 85 ETGTYPEKDDENTNLQVVVDTKAQLNVNVNDGKPNDI 121 >SPAC1565.02c |||GTPase activating protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 374 Score = 25.4 bits (53), Expect = 5.1 Identities = 13/50 (26%), Positives = 29/50 (58%), Gaps = 1/50 (2%) Frame = -2 Query: 329 FTLSMVSSLVSEVRNGFRFAVSVRVTVASTHSNNNVRLVVVVESLL-QLT 183 F+ ++ L + +G +AVS++ + ++ N+ +L ++ SLL Q+T Sbjct: 247 FSAEFLNGLTDHMDSGIDYAVSLQKLIDASMDKNSQKLARLIFSLLYQIT 296 >SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-lysine forming] |Schizosaccharomyces pombe|chr 1|||Manual Length = 368 Score = 25.0 bits (52), Expect = 6.7 Identities = 12/41 (29%), Positives = 21/41 (51%) Frame = +3 Query: 189 LKEALDDDNKPHVIVAVRGSYSYTNTDGKPETITYFADETG 311 LKE ++DN P ++ ++ Y N +G E ++ F G Sbjct: 76 LKELPENDNSPLKHTHIQFAHCYKNQEGWREVLSRFPAGNG 116 >SPBC543.06c |dbp8||ATP-dependent RNA helicase Dbp8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 453 Score = 25.0 bits (52), Expect = 6.7 Identities = 12/52 (23%), Positives = 23/52 (44%) Frame = +3 Query: 132 AYNYNFETSNGIVRSETGELKEALDDDNKPHVIVAVRGSYSYTNTDGKPETI 287 A N + ++ +++++D +PHV+VA G + ETI Sbjct: 97 ALGANLNLKHALIVGGMDMIRQSIDLSKRPHVVVATPGRLADLIRSNGEETI 148 >SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1116 Score = 24.6 bits (51), Expect = 8.9 Identities = 11/27 (40%), Positives = 17/27 (62%) Frame = +1 Query: 295 SLTRLDTMLRVNLFLRSPPPRAKLLNS 375 SL+ L LR + +R PP +K++NS Sbjct: 685 SLSPLSYRLRKSKHIRESPPSSKVINS 711 >SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit Nse6|Schizosaccharomyces pombe|chr 1|||Manual Length = 522 Score = 24.6 bits (51), Expect = 8.9 Identities = 24/101 (23%), Positives = 42/101 (41%), Gaps = 11/101 (10%) Frame = -2 Query: 281 FRFAVSVRVTVASTHSNNNVRLVVVVESLLQLTSFATHDAVGSLEIVVVSSIGAGV--EF 108 +R+ + + + A+ + R + V++LLQ S T ++ ++ S+G+ V Sbjct: 64 YRWNIDLLSSTATIDDSVAKRRKLAVQNLLQYDSTQTFQTGDEIDELIGKSVGSNVLNVL 123 Query: 107 RPN-----DLRVVLCRGGS----DDGSHKGESYNDDQFHFN 12 R N DLR C D + K E D+ FN Sbjct: 124 RSNPIYDDDLRYEYCSNSKARVPDWNTLKAECLKDNDLEFN 164 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 24.6 bits (51), Expect = 8.9 Identities = 17/53 (32%), Positives = 28/53 (52%), Gaps = 3/53 (5%) Frame = -2 Query: 230 NNVRLVV---VVESLLQLTSFATHDAVGSLEIVVVSSIGAGVEFRPNDLRVVL 81 +N+ LVV + S Q+TS ++ SLE ++ +GA R +D +V L Sbjct: 956 SNIALVVDELINLSSKQITSHPQSLSIASLEASLIFVLGASSIIREDDSKVTL 1008 >SPCC584.15c |||arrestin/PY protein 2|Schizosaccharomyces pombe|chr 3|||Manual Length = 594 Score = 24.6 bits (51), Expect = 8.9 Identities = 13/39 (33%), Positives = 21/39 (53%) Frame = +1 Query: 262 TLTANLKPLRTSLTRLDTMLRVNLFLRSPPPRAKLLNSL 378 TLTA+L+ T L+T L+ + PP L++S+ Sbjct: 139 TLTASLERATQPPTNLETALQFRVIRTIPPNSLDLMHSV 177 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,851,616 Number of Sequences: 5004 Number of extensions: 34472 Number of successful extensions: 114 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 113 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 114 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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