BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30134 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 31 0.10 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 31 0.10 SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 27 2.2 SPCC830.08c |||Golgi membrane protein |Schizosaccharomyces pombe... 26 3.8 SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 26 3.8 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 25 5.1 SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 25 6.7 SPCC1620.10 |cwf26||complexed with Cdc5 protein Cwf26 |Schizosac... 25 8.9 >SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 31.1 bits (67), Expect = 0.10 Identities = 23/92 (25%), Positives = 44/92 (47%), Gaps = 4/92 (4%) Frame = +3 Query: 126 ELEIALDHANKANAEAQKNIKRYQAQIKDLQTALXXXXXXXXXXXXXLGI-SERRANALQ 302 E ++ L NAE++K+ R + +++DL ++L + SE+R N L+ Sbjct: 87 EEKLQLTETKCRNAESEKS--RVENELEDLTSSLFEEANRMVANARKETVASEKRVNQLK 144 Query: 303 NELEESRTLLEQADRARRQAE---QELSDAHE 389 +L ++ TLL + + +SD+HE Sbjct: 145 KQLVDAETLLSSTQHQLTELKDVMHSMSDSHE 176 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 31.1 bits (67), Expect = 0.10 Identities = 27/93 (29%), Positives = 39/93 (41%), Gaps = 1/93 (1%) Frame = +3 Query: 102 KKLEADINELEIALDHANKANAEAQKN-IKRYQAQIKDLQTALXXXXXXXXXXXXXLGIS 278 KKLE D+N L L+ N A+ E+ KN I+ + K L L L Sbjct: 753 KKLENDLNLLTEKLNKKN-ADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEE 811 Query: 279 ERRANALQNELEESRTLLEQADRARRQAEQELS 377 R LQ++L + + +E + A ELS Sbjct: 812 STRLQELQSQLNQDKNQIETLNERISAAADELS 844 >SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 1106 Score = 26.6 bits (56), Expect = 2.2 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = +3 Query: 105 KLEADINELEIALDHANKANAEAQKNIKR 191 KL + + L LDH+N +AE + IKR Sbjct: 430 KLGPEYSSLSAILDHSNNIHAEILETIKR 458 >SPCC830.08c |||Golgi membrane protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 182 Score = 25.8 bits (54), Expect = 3.8 Identities = 13/32 (40%), Positives = 20/32 (62%) Frame = +1 Query: 193 TRHKSRICRPLSRRSSAPVTMPANSSASRSVA 288 T H RIC+ +SR+++AP P SS + + A Sbjct: 146 TPHVIRICKSVSRQNAAPA--PTASSFAHTTA 175 >SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1194 Score = 25.8 bits (54), Expect = 3.8 Identities = 12/28 (42%), Positives = 18/28 (64%) Frame = +3 Query: 144 DHANKANAEAQKNIKRYQAQIKDLQTAL 227 D+ N + +A KN+K YQ + DLQ +L Sbjct: 661 DYRN-SRLDAIKNVKTYQIKFSDLQESL 687 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 25.4 bits (53), Expect = 5.1 Identities = 18/101 (17%), Positives = 43/101 (42%), Gaps = 7/101 (6%) Frame = +3 Query: 93 RMKKKLEADINELEIALDHANKANAE-------AQKNIKRYQAQIKDLQTALXXXXXXXX 251 +M K++ + + E +L HA AE K IK ++ +DL+ L Sbjct: 420 KMVKQVSSQLEEARSSLAHATGKLAEINSERDFQNKKIKDFEKIEQDLRACLNSSSNELK 479 Query: 252 XXXXXLGISERRANALQNELEESRTLLEQADRARRQAEQEL 374 + ++ N L+ +++E + + E + + ++++ Sbjct: 480 EKSALIDKKDQELNNLREQIKEQKKVSESTQSSLQSLQRDI 520 >SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 2104 Score = 25.0 bits (52), Expect = 6.7 Identities = 18/74 (24%), Positives = 31/74 (41%) Frame = +3 Query: 168 EAQKNIKRYQAQIKDLQTALXXXXXXXXXXXXXLGISERRANALQNELEESRTLLEQADR 347 + + ++R A I +LQ L L I++ R L N+L+E + L + D Sbjct: 833 QTDEYLRRKDALINNLQNQLESTKEVANE----LTITKERVLQLTNDLQEEQALAHEKDI 888 Query: 348 ARRQAEQELSDAHE 389 +A + HE Sbjct: 889 LVERANSRVEVVHE 902 >SPCC1620.10 |cwf26||complexed with Cdc5 protein Cwf26 |Schizosaccharomyces pombe|chr 3|||Manual Length = 305 Score = 24.6 bits (51), Expect = 8.9 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = +3 Query: 282 RRANALQNELEESRTLLEQADRARRQAEQE 371 RR + + E R L E+ + ARRQ EQ+ Sbjct: 163 RRIDLVLARKEAKRKLKEKEEEARRQKEQQ 192 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.314 0.121 0.379 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,294,582 Number of Sequences: 5004 Number of extensions: 16235 Number of successful extensions: 81 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 78 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 81 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits)
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