BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30132 (516 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g17690.1 68414.m02190 expressed protein 29 2.5 At4g38780.1 68417.m05491 splicing factor, putative strong simila... 27 7.5 At3g04620.1 68416.m00494 expressed protein 27 7.5 >At1g17690.1 68414.m02190 expressed protein Length = 754 Score = 28.7 bits (61), Expect = 2.5 Identities = 22/73 (30%), Positives = 32/73 (43%), Gaps = 5/73 (6%) Frame = -2 Query: 491 SYSHVIHSNIKT-----PTKSSSAMKL*TLEIQLEPRFRSMTNKKKTSQKLIKHLHYIKF 327 SY ++HSN K + SSAM + L F++ KK K+ KH + Sbjct: 271 SYLDILHSNKKPFYHCGSDEDSSAMDAYLMH-SLNHIFKTRDLVKKNESKIAKHRETSEE 329 Query: 326 RTSKDDGDIDRDF 288 DDG +D+ F Sbjct: 330 EILSDDGFLDQGF 342 >At4g38780.1 68417.m05491 splicing factor, putative strong similarity to splicing factor Prp8 [Homo sapiens] GI:3661610; contains Pfam profile PF01398: Mov34/MPN/PAD-1 family Length = 2332 Score = 27.1 bits (57), Expect = 7.5 Identities = 8/17 (47%), Positives = 14/17 (82%) Frame = -2 Query: 341 HYIKFRTSKDDGDIDRD 291 H+++F ++DGD+DRD Sbjct: 2312 HFLQFSKMEEDGDLDRD 2328 >At3g04620.1 68416.m00494 expressed protein Length = 164 Score = 27.1 bits (57), Expect = 7.5 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +1 Query: 58 VRNTRKSLLIYILYNKRYIRFHRD 129 V NT+K L Y+ KRYI+ H + Sbjct: 42 VSNTKKPLFFYVNLAKRYIQQHNE 65 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,041,384 Number of Sequences: 28952 Number of extensions: 154109 Number of successful extensions: 240 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 239 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 240 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 937669760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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