Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30127
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 65 5e-12
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 51 9e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 36 0.005
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 35 0.006
SPBC21H7.07c |his5||imidazoleglycerol phosphate dehydratase|Schi... 31 0.14
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 30 0.18
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 28 0.72
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 26 2.9
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 25 5.1
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 25 5.1
SPBC1604.12 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 6.7
SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 25 8.9
SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccha... 25 8.9
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 65.3 bits (152), Expect = 5e-12
Identities = 38/109 (34%), Positives = 60/109 (55%), Gaps = 6/109 (5%)
Frame = +2
Query: 68 NDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGGSDEDDVVINA 235
N+L SLG+ + EL +M+ E +G I+FT+ LT+ A +M +D ++ V A
Sbjct: 32 NELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMK-DTDNEEEVREA 90
Query: 236 FKTFDEEGK--IDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYID 376
FK FD++G I E L H L + G++ S +EV + + D D G I+
Sbjct: 91 FKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVIN 139
Score = 36.3 bits (80), Expect = 0.003
Identities = 17/35 (48%), Positives = 22/35 (62%)
Frame = +1
Query: 1 QVAEFKEAFQLMDHDKDGIIGKKRLARHLRLPGQA 105
Q+AEF+EAF L D D+DG I L +R GQ+
Sbjct: 10 QIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQS 44
Score = 29.9 bits (64), Expect = 0.24
Identities = 20/57 (35%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
Frame = +2
Query: 248 DEEGKID-SERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTASAE 415
D G ID +E L D + +EV EA+ D D GYI +L +LT+ E
Sbjct: 60 DGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGE 116
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 51.2 bits (117), Expect = 9e-08
Identities = 30/91 (32%), Positives = 46/91 (50%), Gaps = 2/91 (2%)
Frame = +2
Query: 65 RNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKT 244
R D++ SL + ASE ++ M + PIN LT + + S +D ++ AF T
Sbjct: 67 REDVKTMLTSLNQDASEDSINHMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFST 125
Query: 245 FD--EEGKIDSERLRHALMTWGDKFSADEVD 331
FD + GKI +R AL + GD+ EV+
Sbjct: 126 FDDTQSGKIPISTMRDALSSMGDRMDPQEVE 156
Score = 31.5 bits (68), Expect = 0.077
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +1
Query: 1 QVAEFKEAFQLMDHDKDGIIGKK 69
Q+ E KEAF L+D D DG IG++
Sbjct: 46 QIQELKEAFALLDKDGDGNIGRE 68
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 35.5 bits (78), Expect = 0.005
Identities = 25/101 (24%), Positives = 49/101 (48%), Gaps = 4/101 (3%)
Frame = +2
Query: 119 ELDEMVGEASGPINFTQLLTLFANRMSGGS--DEDDVVINAFKTFDEE--GKIDSERLRH 286
E+ E+ ++ Q L + NR +G + + + F+ FD++ G I LR+
Sbjct: 43 EITEIESTLPAEVDMEQFLQVL-NRPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRY 101
Query: 287 ALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTAS 409
L + G+K S +E+DE + + D G ++ + M+ A+
Sbjct: 102 VLTSLGEKLSNEEMDELLKGVPVKD-GMVNYHDFVQMILAN 141
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 35.1 bits (77), Expect = 0.006
Identities = 26/121 (21%), Positives = 55/121 (45%), Gaps = 6/121 (4%)
Frame = +2
Query: 68 NDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGGSDEDDVVINA 235
++LRA +LG A + E+ +++ + G + + + ++ D + + A
Sbjct: 57 HELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVE-RDPLEEIKRA 115
Query: 236 FKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTAS 409
F+ FD++ GKI LR + E++ ++ D+D G I+ + IA++
Sbjct: 116 FELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAIMMDE 175
Query: 410 A 412
A
Sbjct: 176 A 176
Score = 25.4 bits (53), Expect = 5.1
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +1
Query: 1 QVAEFKEAFQLMDHDKDGIIGKKRLARHLRLPG 99
Q + EAF+L D DKD I L +R G
Sbjct: 35 QRQDINEAFKLFDSDKDNAIDYHELRAAMRALG 67
>SPBC21H7.07c |his5||imidazoleglycerol phosphate
dehydratase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 216
Score = 30.7 bits (66), Expect = 0.14
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = -1
Query: 480 GWRLRPYSRDGIITRLHHLPSSSALAVSMA 391
GW LR YSR +I HH +A+A+ +A
Sbjct: 71 GWSLRLYSRGDLIIDDHHTAEDTAIALGIA 100
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 30.3 bits (65), Expect = 0.18
Identities = 16/33 (48%), Positives = 18/33 (54%)
Frame = +1
Query: 1 QVAEFKEAFQLMDHDKDGIIGKKRLARHLRLPG 99
Q E KEAF L D DKDG+I + LR G
Sbjct: 7 QTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLG 39
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 28.3 bits (60), Expect = 0.72
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +2
Query: 320 DEVDEAYDQMDIDDKGYID 376
+E +EA+D D+ KGYID
Sbjct: 12 EEAEEAFDLFDVTHKGYID 30
>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 457
Score = 26.2 bits (55), Expect = 2.9
Identities = 10/28 (35%), Positives = 20/28 (71%)
Frame = -1
Query: 144 ASPTISSSSFSDASLPRESKVARKSFLA 61
+SP++ +++ + P++S V+RKSF A
Sbjct: 35 SSPSVPEGTYTVLNNPKQSTVSRKSFSA 62
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 25.4 bits (53), Expect = 5.1
Identities = 18/70 (25%), Positives = 30/70 (42%), Gaps = 2/70 (2%)
Frame = -3
Query: 220 VIFV--RAAGHAVGEQSQELSEVDGARSLTDHLIELLLGREPAQGVEGGAQVVSCR*CHP 47
+IFV A + + E QEL + R + D L+ +L ++ G AQ+
Sbjct: 92 LIFVVDSADSNRISEARQELHRIISDREMRDCLLLVLANKQDLPGALSPAQITDVLQLDK 151
Query: 46 CRDPLAGMPP 17
+D L + P
Sbjct: 152 LKDRLWNVQP 161
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 25.4 bits (53), Expect = 5.1
Identities = 21/49 (42%), Positives = 28/49 (57%), Gaps = 2/49 (4%)
Frame = -1
Query: 210 SEPPDMRLANRVK-S*VKL-MGPEASPTISSSSFSDASLPRESKVARKS 70
S P R+A+ +K S VKL + P+A + SS+ S ASL ES R S
Sbjct: 542 SRPRLPRVASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSS 590
>SPBC1604.12 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 860
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = +3
Query: 39 SRQGWHHRQETTCAPPSTPWAGSRP 113
S +G H QETT PS+P S P
Sbjct: 586 SPEGLHSNQETTEIDPSSPRDDSTP 610
>SPCC132.04c |||NAD-dependent glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1106
Score = 24.6 bits (51), Expect = 8.9
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +2
Query: 248 DEEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDD 361
D++ K+ + LR T D DEVD Y+++ +DD
Sbjct: 103 DQKSKV-IQCLRTERKTIPDDLVEDEVDRFYNKLGLDD 139
>SPCC1322.14c |vtc4||vacuolar transporter chaperone
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 721
Score = 24.6 bits (51), Expect = 8.9
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +2
Query: 248 DEEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDK 364
DEE +I S R+ T+ S E+DE + +DI +
Sbjct: 524 DEESRIGSSSTRNDNSTFQTSDSFQELDERTNLLDISKR 562
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,691,378
Number of Sequences: 5004
Number of extensions: 27055
Number of successful extensions: 139
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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