Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30119
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 173 8e-44
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 169 1e-42
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 166 9e-42
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 162 1e-40
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 100 7e-22
At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes... 29 1.9
At2g33420.1 68415.m04096 expressed protein 29 2.5
At5g45610.1 68418.m05605 expressed protein 28 3.2
At1g68750.1 68414.m07859 phosphoenolpyruvate carboxylase family ... 28 3.2
At5g10520.1 68418.m01218 protein kinase family protein contains ... 28 4.3
At5g29613.1 68418.m03639 hypothetical protein 27 5.7
At3g23270.1 68416.m02933 regulator of chromosome condensation (R... 27 5.7
At5g49430.1 68418.m06116 transducin family protein / WD-40 repea... 27 7.5
At1g13770.1 68414.m01616 expressed protein contains Pfam domain,... 27 7.5
At3g53320.1 68416.m05883 expressed protein 27 9.9
At3g02750.1 68416.m00267 protein phosphatase 2C family protein /... 27 9.9
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 173 bits (420), Expect = 8e-44
Identities = 92/160 (57%), Positives = 108/160 (67%), Gaps = 1/160 (0%)
Frame = +3
Query: 39 AFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDK-DRLTWDIHFWLG 215
AF AG+KPG EIWRIENFE V VPK++ G FY GD+YIVL TT +K +DIHFW+G
Sbjct: 9 AFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIG 68
Query: 216 SRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGHASGF 395
TSQDEAG AA+ TV L D G AVQHRE+Q +ES +FL YF P I L+GG ASGF
Sbjct: 69 KDTSQDEAGTAAVKTVEL-DAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLEGGVASGF 127
Query: 396 SHVTINEGTEKRLFQIKGKRNVRVKQVKPTFESLNNGDCF 515
V E E RL+ KGKR +R+KQV SLN+ D F
Sbjct: 128 KTVE-EEVFETRLYTCKGKRAIRLKQVPFARSSLNHDDVF 166
Score = 64.1 bits (149), Expect = 5e-11
Identities = 48/160 (30%), Positives = 75/160 (46%), Gaps = 11/160 (6%)
Frame = +3
Query: 69 LEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRL-TWDIHFWLGSRTSQDEAGA 245
LE+W + +PK G YSGD Y+VL T +R + + W G ++ ++
Sbjct: 392 LEVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGERKDEYFLSCWFGKKSIPEDQD- 450
Query: 246 AAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGHASGF-SHVTINEGT 422
AI N +G VQ R + E +F+ F P + LKGG +SG+ S + +E T
Sbjct: 451 TAIRLANTMSNSLKGRPVQGRIYEGKEPPQFVALFQPMV-VLKGGLSSGYKSSMGESEST 509
Query: 423 EK-------RLFQIKGK--RNVRVKQVKPTFESLNNGDCF 515
++ L Q+ G N + QV+ SLN+ +CF
Sbjct: 510 DETYTPESIALVQVSGTGVHNNKAVQVETVATSLNSYECF 549
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 169 bits (411), Expect = 1e-42
Identities = 90/160 (56%), Positives = 106/160 (66%), Gaps = 1/160 (0%)
Frame = +3
Query: 39 AFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDK-DRLTWDIHFWLG 215
AF G+KPG EIWRIENFEPV VPK++ G FY GD+YIVL TT +K +DIHFW+G
Sbjct: 11 AFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIG 70
Query: 216 SRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGHASGF 395
TSQDEAG AA+ TV L D G AVQ+RE+Q +ES +FL YF P I L+GG ASGF
Sbjct: 71 KDTSQDEAGTAAVKTVEL-DAALGGRAVQYREIQGHESDKFLSYFKPCIIPLEGGVASGF 129
Query: 396 SHVTINEGTEKRLFQIKGKRNVRVKQVKPTFESLNNGDCF 515
E E RL+ KGKR V +KQV SLN+ D F
Sbjct: 130 KKPE-EEEFETRLYTCKGKRAVHLKQVPFARSSLNHDDVF 168
Score = 61.3 bits (142), Expect = 4e-10
Identities = 46/160 (28%), Positives = 73/160 (45%), Gaps = 11/160 (6%)
Frame = +3
Query: 69 LEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRL-TWDIHFWLGSRTSQDEAGA 245
LE+W I+ + K G YSGD Y+VL T +R + + W G ++Q++
Sbjct: 394 LEVWYIDANSKTVLSKDHVGKLYSGDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQET 453
Query: 246 AAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGHASGFSHVTINEGTE 425
A L + + +G VQ R + E +F+ F + LKGG +SG+ + +G+
Sbjct: 454 AVRLASTMTN-SLKGRPVQARIFEGKEPPQFVALFQHMV-VLKGGLSSGYKNSMTEKGSS 511
Query: 426 KR--------LFQIKGK--RNVRVKQVKPTFESLNNGDCF 515
L Q+ G N + QV+ SLN+ DCF
Sbjct: 512 GETYTPESIALIQVSGTGVHNNKALQVEAVATSLNSYDCF 551
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 166 bits (403), Expect = 9e-42
Identities = 87/166 (52%), Positives = 112/166 (67%), Gaps = 1/166 (0%)
Frame = +3
Query: 21 MPEVHQAFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKD-RLTWD 197
M ++ QA AG+K G+EIWRIENF+PV VP+ G F++GDSYIVL TT + L D
Sbjct: 5 MRDLDQALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHD 64
Query: 198 IHFWLGSRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKG 377
IH+WLG +SQDEAGA A++TV L D G AVQ+REVQ +E+++FL YF P I +G
Sbjct: 65 IHYWLGKDSSQDEAGAVAVMTVEL-DSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEG 123
Query: 378 GHASGFSHVTINEGTEKRLFQIKGKRNVRVKQVKPTFESLNNGDCF 515
G ASGF+HV E + RL+ KGK VRVK+V +LN+ D F
Sbjct: 124 GVASGFNHVKPEE-HQTRLYICKGKHVVRVKEVPFVRSTLNHEDVF 168
Score = 48.4 bits (110), Expect = 3e-06
Identities = 42/159 (26%), Positives = 68/159 (42%), Gaps = 10/159 (6%)
Frame = +3
Query: 69 LEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRLTWDIHFWLGSRTSQDEAGAA 248
L++WRI E + + + FYSGD YI+ + +DR + W G + S +E A+
Sbjct: 396 LQVWRINCEEKILLEAAEQSKFYSGDCYILQYSYPGEDREEHLVGTWFG-KQSVEEDRAS 454
Query: 249 AILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGHASGFSHV-------- 404
AI N E + Q R + E +F I + KGG + F
Sbjct: 455 AISLANKMVESMKFVPAQARINEGKEPIQFFVIMQSFITF-KGGVSDAFKKYIAENDIPD 513
Query: 405 TINEGTEKRLFQIKGK--RNVRVKQVKPTFESLNNGDCF 515
T E LF+++G N++ Q++ LN+ C+
Sbjct: 514 TTYEAEGVALFRVQGSGPENMQAIQIEAASAGLNSSHCY 552
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 162 bits (394), Expect = 1e-40
Identities = 87/166 (52%), Positives = 107/166 (64%), Gaps = 1/166 (0%)
Frame = +3
Query: 21 MPEVHQAFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDK-DRLTWD 197
M ++ AF AG+K G+EIWRIENF P +PK+ G F++GDSYIVL TT K L D
Sbjct: 5 MRDLDPAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGALRHD 64
Query: 198 IHFWLGSRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKG 377
IH+WLG TSQDEAG AA+ TV L D G AVQ+REVQ +E+++FL YF P I +G
Sbjct: 65 IHYWLGKDTSQDEAGTAAVKTVEL-DAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEG 123
Query: 378 GHASGFSHVTINEGTEKRLFQIKGKRNVRVKQVKPTFESLNNGDCF 515
G ASGF HV E RLF +GK V VK+V SLN+ D +
Sbjct: 124 GVASGFKHVVAEEHI-TRLFVCRGKHVVHVKEVPFARSSLNHDDIY 168
Score = 50.8 bits (116), Expect = 5e-07
Identities = 42/171 (24%), Positives = 77/171 (45%), Gaps = 12/171 (7%)
Frame = +3
Query: 27 EVHQAFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRLTWDIHF 206
E Q F D L++WR+ + FYSGD Y+ + +++ I
Sbjct: 382 EEPQVFIDC--TGNLQVWRVNGQAKTLLQAADHSKFYSGDCYVFQYSYPGEEKEEVLIGT 439
Query: 207 WLGSRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYF--SPAIRYLKGG 380
W G ++ ++E G+A V++ + + + + YE KE +++F + KGG
Sbjct: 440 WFGKQSVEEERGSA----VSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVFKGG 495
Query: 381 HASGF----SHVTINEGTEKR----LFQIKGK--RNVRVKQVKPTFESLNN 503
+SG+ + +++ T LF+I+G N++ QV P SLN+
Sbjct: 496 ISSGYKKYIAEKEVDDDTYNENGVALFRIQGSGPENMQAIQVDPVAASLNS 546
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 100 bits (239), Expect = 7e-22
Identities = 59/164 (35%), Positives = 93/164 (56%), Gaps = 1/164 (0%)
Frame = +3
Query: 27 EVHQAFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDK-DRLTWDIH 203
++ AF G K GLEIW + N + +++PK+ FG F+SG++Y+VL T K + +DIH
Sbjct: 7 DIDSAFQGVGTKSGLEIWCVYNKQLISIPKSSFGKFHSGNAYLVLRTFLRKIESPQYDIH 66
Query: 204 FWLGSRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGH 383
+WLG ++ ++ A+ ++L D VQ+REVQ E+++FL YF P I ++G +
Sbjct: 67 YWLGIDANEVDSILASDKALDL-DAALGCCTVQYREVQGQETEKFLSYFKPCIIPVEGKY 125
Query: 384 ASGFSHVTINEGTEKRLFQIKGKRNVRVKQVKPTFESLNNGDCF 515
+ E + L + KG VRVK+V SLN+ D F
Sbjct: 126 SPKTG--IAGETYQVTLLRCKGDHVVRVKEVPFLRSSLNHDDVF 167
Score = 32.7 bits (71), Expect = 0.15
Identities = 36/158 (22%), Positives = 66/158 (41%), Gaps = 10/158 (6%)
Frame = +3
Query: 60 KPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRLTWDIHFWLGSRTSQDEA 239
+ L++WR++ + + ++GD Y+V K+R ++ W+G + Q +
Sbjct: 388 RDNLKVWRVDGDDVSLLSIPDQTKLFTGDCYLVQYKYTYKERTEHLLYVWIGCESIQQDR 447
Query: 240 GAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGHASGFSHVTINE- 416
A AI + +G +V Q E F F + + KGG + + + +
Sbjct: 448 -ADAITNASAIVGTTKGESVLCHIYQGNEPSRFFPMFQSLVVF-KGGLSRRYKVLLAEKE 505
Query: 417 --GTE-----KRLFQIKG--KRNVRVKQVKPTFESLNN 503
G E LF++ G RN++ QV SLN+
Sbjct: 506 KIGEEYNENKASLFRVVGTSPRNMQAIQVNLVATSLNS 543
>At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes
from this gene
Length = 1035
Score = 29.1 bits (62), Expect = 1.9
Identities = 25/86 (29%), Positives = 38/86 (44%)
Frame = +1
Query: 61 SRVSKYGGLRTSNRSPFRRLSSVSSTPGTPTLY*TPQAIRTV*HGTSISGSARGPARTKP 240
SRV + GL+ RSP RR+S+V + G + +P ++ G+ G P
Sbjct: 107 SRVKRALGLKMLKRSPSRRMSTVGTVVGAVSAPSSPGNNGSIGSGSGHFSPGAGFFTVPP 166
Query: 241 ARRPSSR*TWTTNNSRDQRYSTERSN 318
AR R + R Q TE+S+
Sbjct: 167 AR--PRRPLTSAEIMRQQMKVTEQSD 190
>At2g33420.1 68415.m04096 expressed protein
Length = 1039
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = +1
Query: 61 SRVSKYGGLRTSNRSPFRRLSSVSSTPGTPT 153
SRV + GL+ RSP RR+S++ + G T
Sbjct: 113 SRVKRALGLKMLKRSPSRRMSTIGAAGGAAT 143
>At5g45610.1 68418.m05605 expressed protein
Length = 633
Score = 28.3 bits (60), Expect = 3.2
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = -2
Query: 137 VEETELSLRNGDRFEVLNPPYFETRLPA 54
++ E +L+ D + L PPY T LPA
Sbjct: 16 IDSIETTLKKADMYRPLPPPYLPTFLPA 43
>At1g68750.1 68414.m07859 phosphoenolpyruvate carboxylase family
protein / PEP carboxylase family protein similar to
SP|P51059 Phosphoenolpyruvate carboxylase 2 (EC
4.1.1.31) (PEPCASE) {Zea mays}; contains Pfam profile
PF00311: phosphoenolpyruvate carboxylase
Length = 1032
Score = 28.3 bits (60), Expect = 3.2
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +3
Query: 276 EQFQGSAVQHREVQYYESKEFLEYFSPA 359
E+ G + QH YE+ EFL YF A
Sbjct: 812 EEISGISCQHYRSTVYENPEFLSYFHEA 839
>At5g10520.1 68418.m01218 protein kinase family protein contains
protein kinase domain, INTERPRO:IPR000719
Length = 467
Score = 27.9 bits (59), Expect = 4.3
Identities = 15/39 (38%), Positives = 18/39 (46%)
Frame = +3
Query: 306 REVQYYESKEFLEYFSPAIRYLKGGHASGFSHVTINEGT 422
R Y E +YF+P KGGHA + V IN T
Sbjct: 139 RNFTYEELAVATDYFNPENMIGKGGHAEVYKGVLINGET 177
>At5g29613.1 68418.m03639 hypothetical protein
Length = 117
Score = 27.5 bits (58), Expect = 5.7
Identities = 16/63 (25%), Positives = 29/63 (46%)
Frame = +3
Query: 267 LDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGHASGFSHVTINEGTEKRLFQIK 446
LD Q Q H + ++ + F E F + A+G + + + +GT+ R +I
Sbjct: 21 LDGIQTQNGLQHHMSYKKFKDETF-EEFDDLKMVFEDNIATGGNAIGLGDGTDARTCEIA 79
Query: 447 GKR 455
GK+
Sbjct: 80 GKK 82
>At3g23270.1 68416.m02933 regulator of chromosome condensation
(RCC1) family protein contains Pfam domain PF00415:
Regulator of chromosome condensation (RCC1); similar to
zinc finger protein (GI:15811367) [Arabidopsis
thaliana]; similar to chromosome condensation regulator
protein (GI:22770461) [Cicer arietinum]
Length = 1045
Score = 27.5 bits (58), Expect = 5.7
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = -3
Query: 103 TGSKFSILHISRPGFLPASANA 38
+ +K S++ SRPGF P S+NA
Sbjct: 701 SANKNSVMSSSRPGFTPESSNA 722
>At5g49430.1 68418.m06116 transducin family protein / WD-40 repeat
family protein similar to WD-repeat protein 9
(SP:Q9NSI6) {Homo sapiens}; contains Pfam PF00400: WD
domain, G-beta repeat (4 copies)
Length = 1677
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/33 (39%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
Frame = -3
Query: 112 GTATGSKFSILHISRPGFLPASANAW-CTSGIL 17
G A GSKFS+ S+ +P N+W C I+
Sbjct: 457 GCAAGSKFSVTDYSKDENVPKFKNSWFCHDNIV 489
>At1g13770.1 68414.m01616 expressed protein contains Pfam domain,
PF04884: Protein of unknown function, DUF647; contains
non-consensus (GC) splice site at intron 2
Length = 440
Score = 27.1 bits (57), Expect = 7.5
Identities = 18/46 (39%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -3
Query: 298 TADPWNCSSSKFTVRMAAAPASSWLVLEPSQKWMSHV-RRSLSPVV 164
T + WN SSS + A ASS L ++ S +HV RR L V
Sbjct: 13 TLEEWNGSSSTKLFKTATITASSSLSIQRSANRFNHVWRRVLQAFV 58
>At3g53320.1 68416.m05883 expressed protein
Length = 553
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/48 (31%), Positives = 28/48 (58%)
Frame = +1
Query: 190 HGTSISGSARGPARTKPARRPSSR*TWTTNNSRDQRYSTERSNITSPR 333
HG+S S S + +T+PAR P +TN+ + S++ +++SP+
Sbjct: 494 HGSSSSASKKSGGKTQPARSPIQE---STNSK--SKASSKVVSVSSPK 536
>At3g02750.1 68416.m00267 protein phosphatase 2C family protein /
PP2C family protein similar to protein phosphatase-2C;
PP2C (GI:3643088) [Mesembryanthemum crystallinum];
contains Pfam PF00481 : Protein phosphatase 2C domain;
Length = 492
Score = 26.6 bits (56), Expect = 9.9
Identities = 24/84 (28%), Positives = 38/84 (45%), Gaps = 7/84 (8%)
Frame = +3
Query: 24 PEVHQAFADAGRKPGLEIWR------IENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDR 185
PEV + + PGL + R +++F ++VP F D +IVL T G D
Sbjct: 284 PEVCRVWLPNCDSPGLAMARAFGDFCLKDFGLISVPDVSFRQLTEKDEFIVLATDGIWDV 343
Query: 186 LT-WDIHFWLGSRTSQDEAGAAAI 254
L+ D+ + S S+ A A +
Sbjct: 344 LSNEDVVAIVASAPSRSSAARALV 367
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,649,334
Number of Sequences: 28952
Number of extensions: 240842
Number of successful extensions: 762
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 745
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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