BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30114 (516 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / sta... 114 4e-26 At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / sta... 107 4e-24 At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 44 5e-05 At3g06290.1 68416.m00722 SAC3/GANP family protein contains Pfam ... 31 0.46 At2g21710.1 68415.m02582 mitochondrial transcription termination... 29 1.4 At3g51770.1 68416.m05677 tetratricopeptide repeat (TPR)-containi... 28 3.2 At3g28960.1 68416.m03620 amino acid transporter family protein l... 27 5.7 At2g27980.1 68415.m03391 expressed protein 27 5.7 At1g25530.1 68414.m03170 lysine and histidine specific transport... 27 5.7 At2g40810.2 68415.m05035 WD-40 repeat protein family similar to ... 27 7.5 At2g40810.1 68415.m05034 WD-40 repeat protein family similar to ... 27 7.5 At5g50280.1 68418.m06226 pentatricopeptide (PPR) repeat-containi... 27 9.9 >At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / starch branching enzyme class II (SBE2-2) identical to starch branching enzyme class II [Arabidopsis thaliana] GI:726490 Length = 716 Score = 114 bits (274), Expect = 4e-26 Identities = 55/126 (43%), Positives = 78/126 (61%), Gaps = 1/126 (0%) Frame = +1 Query: 112 PMEVPVP-DLEKLLERDGYLKPYEREIRRRYACYKDIWDRIESWDGGLEAFTKGYKYYGP 288 P VP P D +K+ E D L+ Y + RY YK + + I+ ++GGLEAF++GY+ G Sbjct: 24 PRIVPPPGDGKKIYEIDPMLRTYNNHLDYRYGQYKRLREEIDKYEGGLEAFSRGYEKLGF 83 Query: 289 QFNQDGSVTWREWAPGAHSLHLRGEFNGWDSKSHPFAKKEYGKWEIQIPANPDGSCALKH 468 D +T+REWAPGA + L G+FN W+S + + E+G WEI +P N DGS A+ H Sbjct: 84 S-RSDAGITYREWAPGAKAASLIGDFNNWNSNADIMTRNEFGVWEIFLPNNTDGSPAIPH 142 Query: 469 DSRVQI 486 SRV+I Sbjct: 143 GSRVKI 148 >At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / starch branching enzyme class II (SBE2-1) nearly identical to starch branching enzyme class II [Arabidopsis thaliana] GI:619939 Length = 858 Score = 107 bits (257), Expect = 4e-24 Identities = 50/124 (40%), Positives = 80/124 (64%), Gaps = 1/124 (0%) Frame = +1 Query: 118 EVPVP-DLEKLLERDGYLKPYEREIRRRYACYKDIWDRIESWDGGLEAFTKGYKYYGPQF 294 ++P P D +++ + D L + + RY Y+ + + I+ +GGLEAF++GY+ +G Sbjct: 150 KIPPPGDGKRIYDIDPMLNSHRNHLDYRYGQYRKLREEIDKNEGGLEAFSRGYEIFGFTR 209 Query: 295 NQDGSVTWREWAPGAHSLHLRGEFNGWDSKSHPFAKKEYGKWEIQIPANPDGSCALKHDS 474 + G +T+REWAPGA + L G+FN W++KS A+ ++G WEI +P N DGS A+ H S Sbjct: 210 SATG-ITYREWAPGAKAASLIGDFNNWNAKSDVMARNDFGVWEIFLPNNADGSPAIPHGS 268 Query: 475 RVQI 486 RV+I Sbjct: 269 RVKI 272 >At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein similar to 1,4-alpha-glucan branching enzyme [Solanum tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme (EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum tuberosum} SP|P30924; contains Pfam profiles: PF00128 Alpha amylase catalytic domain, PF02922 Isoamylase N-terminal domain Length = 777 Score = 44.4 bits (100), Expect = 5e-05 Identities = 23/85 (27%), Positives = 38/85 (44%), Gaps = 5/85 (5%) Frame = +1 Query: 187 IRRRYACYKDIWDRIESWDGGLEAFTKGYKYYGPQFNQDGSVTWREWAPGAHSLHLRGEF 366 +R R+ KD+ D I F G++ G + + V + +W PG+ + G+F Sbjct: 89 LRERHKALKDLKDEIFKRHFDFRDFASGFELLGMHRHMEHRVDFMDWGPGSRYGAIIGDF 148 Query: 367 NGWD-----SKSHPFAKKEYGKWEI 426 NGW ++ F +YG W I Sbjct: 149 NGWSPTENAAREGLFGHDDYGYWFI 173 >At3g06290.1 68416.m00722 SAC3/GANP family protein contains Pfam profile: PF03399 SAC3/GANP family Length = 1720 Score = 31.1 bits (67), Expect = 0.46 Identities = 14/38 (36%), Positives = 20/38 (52%) Frame = +3 Query: 165 FEAIRKRN*KEICLLQRHLGPYRIMGRWLRSIHEGLQI 278 F RK + + CL+ H R+ + L S+H GLQI Sbjct: 714 FRLARKASYLQACLMHAHFSKVRLRTQALASLHSGLQI 751 >At2g21710.1 68415.m02582 mitochondrial transcription termination factor-related / mTERF-related contains Pfam profile PF02536: mTERF Length = 640 Score = 29.5 bits (63), Expect = 1.4 Identities = 16/45 (35%), Positives = 24/45 (53%) Frame = +3 Query: 129 TRFREIIRKRRLFEAIRKRN*KEICLLQRHLGPYRIMGRWLRSIH 263 + F ++R + E R K IC+ + +L RIM WL+SIH Sbjct: 212 SNFVPLVRWLKHHELSYNRIAKIICMSKGNLDSIRIMIEWLKSIH 256 >At3g51770.1 68416.m05677 tetratricopeptide repeat (TPR)-containing protein contains Pfam profile PF00515: TPR Domain Length = 958 Score = 28.3 bits (60), Expect = 3.2 Identities = 17/45 (37%), Positives = 20/45 (44%) Frame = +3 Query: 39 LANCICKYIHNYLQYNGW*LFSYGSDGSACTRFREIIRKRRLFEA 173 LA K H L Y GW L+ G A + E I +R FEA Sbjct: 696 LARNHSKSEHERLVYEGWILYDTGHREEALAKAEESISIQRSFEA 740 >At3g28960.1 68416.m03620 amino acid transporter family protein low similarity to vesicular inhibitory amino acid transporter [Mus musculus] GI:2826776; contains INTERPRO:IPR002422 amino acid/polyamine transporter, family II Length = 405 Score = 27.5 bits (58), Expect = 5.7 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = -2 Query: 473 ESCFNAHEPSGFAGI*ISHLPYSFLANGWL 384 ++CFNA + +GI I +PYS GWL Sbjct: 20 KTCFNAL--NALSGIGILSVPYSLARGGWL 47 >At2g27980.1 68415.m03391 expressed protein Length = 1037 Score = 27.5 bits (58), Expect = 5.7 Identities = 10/21 (47%), Positives = 14/21 (66%) Frame = -3 Query: 466 VSMHTNHLDLRGFESPICRTP 404 V MH N +++ GF+SP TP Sbjct: 344 VDMHANDVEMDGFQSPSVTTP 364 >At1g25530.1 68414.m03170 lysine and histidine specific transporter, putative similar to lysine and histidine specific transporter GI:2576361 from [Arabidopsis thaliana]; contains Pfam profile PF01490: Transmembrane amino acid transporter protein Length = 440 Score = 27.5 bits (58), Expect = 5.7 Identities = 25/125 (20%), Positives = 50/125 (40%), Gaps = 8/125 (6%) Frame = -1 Query: 513 YPVQVVVHY----DLDTGVVFQCTRTIWICGDLNLPFAVLLFSEWMALRVPSIEFTSKM* 346 +PV ++ ++ D+D V+ R W+ NL V + + +P + +M Sbjct: 274 FPVALICYWAFGQDVDDNVLMNLQRPAWLIAAANLMVVVHVIGSYQVFAMPVFDLLERM- 332 Query: 345 AMRSWSPLAPRNGAILIKLGSVVFVALRECF*ATVP*FDTVPNVFVA-SISPSNF---SF 178 + ++G +L ++VA + P F + F +P++F S Sbjct: 333 ---MVNKFGFKHGVVLRFFTRTIYVAFTLFIGVSFPFFGDLLGFFGGFGFAPTSFFLPSI 389 Query: 177 VWLQI 163 +WL I Sbjct: 390 MWLII 394 >At2g40810.2 68415.m05035 WD-40 repeat protein family similar to Gsa12p(GI:18307769)[Pichia pastoris]; contains 3 Pfam PF00400: WD domain, G-beta repeats Length = 393 Score = 27.1 bits (57), Expect = 7.5 Identities = 11/30 (36%), Positives = 15/30 (50%) Frame = +1 Query: 421 EIQIPANPDGSCALKHDSRVQIIVNDNLYR 510 +I+ ANP G C L H S ++ L R Sbjct: 152 QIETQANPRGLCCLSHHSNTSVLACPGLNR 181 >At2g40810.1 68415.m05034 WD-40 repeat protein family similar to Gsa12p(GI:18307769)[Pichia pastoris]; contains 3 Pfam PF00400: WD domain, G-beta repeats Length = 393 Score = 27.1 bits (57), Expect = 7.5 Identities = 11/30 (36%), Positives = 15/30 (50%) Frame = +1 Query: 421 EIQIPANPDGSCALKHDSRVQIIVNDNLYR 510 +I+ ANP G C L H S ++ L R Sbjct: 152 QIETQANPRGLCCLSHHSNTSVLACPGLNR 181 >At5g50280.1 68418.m06226 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 723 Score = 26.6 bits (56), Expect = 9.9 Identities = 10/32 (31%), Positives = 18/32 (56%) Frame = -1 Query: 462 QCTRTIWICGDLNLPFAVLLFSEWMALRVPSI 367 +C + + G+ + L F EWM+L+ PS+ Sbjct: 204 ECVEALVMMGESGFVKSCLYFYEWMSLQEPSL 235 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,642,752 Number of Sequences: 28952 Number of extensions: 288712 Number of successful extensions: 690 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 675 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 688 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 937669760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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