Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30108
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual      68   7e-13
SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein...    64   1e-11
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ...    31   0.077
SPAC13G7.03 |||up-frameshift suppressor3 family|Schizosaccharomy...    28   0.95 
SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1||...    27   1.3  
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma...    26   3.8  
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch...    26   3.8  
SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomy...    25   6.7  
SPAC8F11.08c |||esterase/lipase|Schizosaccharomyces pombe|chr 1|...    25   6.7  
SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces po...    25   6.7  

>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1489

 Score = 68.1 bits (159), Expect = 7e-13
 Identities = 41/132 (31%), Positives = 66/132 (50%), Gaps = 1/132 (0%)
 Frame = +3

Query: 6   SQHSEKALTQSHNMSLERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDVLKD 185
           SQ      T+  N  L  + R  + +     +  EA++WIE  LG    P   FE  L++
Sbjct: 12  SQAGAPVGTKGSNTRLAAKQRETLQAYDYLCRVDEAKKWIEECLGTDLGPTSTFEQSLRN 71

Query: 186 GTVLCQLINKLKPGSVPKINTTGG-QFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKK 362
           G VL  L+ K +P  + KI  +   QF+  +NI  F   I   G+P+I  F+  D++E K
Sbjct: 72  GVVLALLVQKFQPDKLIKIFYSNELQFRHSDNINKFLDFIHGIGLPEIFHFELTDIYEGK 131

Query: 363 DIAQVVSTLFAL 398
           ++ +V+  + AL
Sbjct: 132 NLPKVIYCIHAL 143


>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
           Stg1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 174

 Score = 64.1 bits (149), Expect = 1e-11
 Identities = 42/128 (32%), Positives = 66/128 (51%), Gaps = 1/128 (0%)
 Frame = +3

Query: 102 EKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENI 281
           EKEA+EWIE  L  K        D L+ G +LC++  +    ++ +   +   F  MENI
Sbjct: 6   EKEAREWIEETLHTKLNAQLDLLDQLQSGVILCRICKEALGANI-RYKESNMPFVQMENI 64

Query: 282 TNF-QSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRHAEWSGPCLGPKPA 458
           + F   A +   VP  D+FQT DL+E+++  QV+ ++ +  R   +        LGPK A
Sbjct: 65  SAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKLA 124

Query: 459 DECKRDFS 482
           ++  R FS
Sbjct: 125 EKKPRVFS 132


>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 962

 Score = 31.5 bits (68), Expect = 0.077
 Identities = 28/101 (27%), Positives = 49/101 (48%), Gaps = 4/101 (3%)
 Frame = +3

Query: 108 EAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITN 287
           EA++W+E     ++   + F D L +G VLCQL  K  P      +    ++++ E  T 
Sbjct: 69  EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYP---KLASNWKPRYQISERNTV 125

Query: 288 FQSA----IKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 398
           + +A    +   G+     F+T DL  + +I +V+  L AL
Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166


>SPAC13G7.03 |||up-frameshift suppressor3 family|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 278

 Score = 27.9 bits (59), Expect = 0.95
 Identities = 20/81 (24%), Positives = 35/81 (43%)
 Frame = +3

Query: 18  EKALTQSHNMSLERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVL 197
           EK  ++    S++ + + ++A K      K  +   E    +K  P E    V+K+  V 
Sbjct: 165 EKGKSKPSKKSVKAKKKLRLAEKPASNNSKAGKSSQESKKSSK-APAESAAAVIKEDKVS 223

Query: 198 CQLINKLKPGSVPKINTTGGQ 260
            +  +K KP   P  N+T  Q
Sbjct: 224 DRKKSKKKPKKTPVSNSTASQ 244


>SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 992

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 18/48 (37%), Positives = 22/48 (45%), Gaps = 4/48 (8%)
 Frame = -3

Query: 226 PGFSLLMSWQR----TVPSLRTSSNSSPGGNLAPRTPSIHSWASFSFS 95
           PG   L   QR    T P L TSS++ PGG     + + HS    S S
Sbjct: 210 PGVGTLQQPQRAGSDTFPDLNTSSSNQPGGEPNVASANTHSLEILSSS 257


>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 614

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 31/108 (28%), Positives = 47/108 (43%), Gaps = 16/108 (14%)
 Frame = +3

Query: 90  NPEKEKEAQEWIEGVL------GAKFPPG-ELFE--DVLKDGTVLCQLINKLKPGSV--- 233
           N E+ +E  + I  VL      G++ P   E FE  D  KDG +L +LIN   P ++   
Sbjct: 111 NEEERREFIKHINSVLAGDPDVGSRVPINTETFEFFDQCKDGLILSKLINDSVPDTIDER 170

Query: 234 ----PKINTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKD 365
                + N     FK +EN     ++ KA G   I      D+ E ++
Sbjct: 171 VLNKQRNNKPLDNFKCIENNNVVINSAKAMGGISITNIGAGDILEGRE 218


>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
           Pss1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 720

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 16/41 (39%), Positives = 24/41 (58%)
 Frame = +3

Query: 54  ERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDV 176
           ER+  AK A+K   EK+K + ++ EG  G + PP    E+V
Sbjct: 665 EREA-AKAATKSEAEKQKPSGKFEEGT-GGRAPPPPPAEEV 703


>SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 589

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 10/45 (22%), Positives = 20/45 (44%)
 Frame = +2

Query: 233 PQDQHHRWTVQNDGEHHKLPVCNQSIRCTRHRCVPNRRSMGEKRH 367
           P+     +++++  +HHKL     +      RC P      +K+H
Sbjct: 206 PRSSTTSYSIRDADKHHKLTTSGVTKMNITERCKPKTTIQTDKKH 250


>SPAC8F11.08c |||esterase/lipase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 376

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 12/39 (30%), Positives = 19/39 (48%)
 Frame = -2

Query: 470 FAFVSGLGSEAGAAPFRVSVSLTTEGKQCADYLGDVFFL 354
           FAFVS    EA +  F  ++     G Q + +   +FF+
Sbjct: 51  FAFVSEKSLEAASVKFVATIIAERMGAQFSSFYDKIFFV 89


>SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1159

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 12/24 (50%), Positives = 17/24 (70%)
 Frame = -2

Query: 458 SGLGSEAGAAPFRVSVSLTTEGKQ 387
           SG G+ AG++PF  S +LT E K+
Sbjct: 656 SGFGNTAGSSPF--SFNLTKESKE 677


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,305,965
Number of Sequences: 5004
Number of extensions: 50642
Number of successful extensions: 144
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)