BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30107 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 27 2.2 SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharom... 25 6.7 SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces... 25 8.9 SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA methyltransfera... 25 8.9 >SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1336 Score = 26.6 bits (56), Expect = 2.2 Identities = 13/39 (33%), Positives = 22/39 (56%) Frame = -3 Query: 289 PLFAPFVDVFLQLFIQVRPLLVLTLDEFWISLTLLFRSG 173 P+FA + L LF+Q+ P + + FW S+ L+ +G Sbjct: 796 PIFAYVISKCLNLFMQIDPSIGVA---FWSSMVLVVAAG 831 >SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharomyces pombe|chr 1|||Manual Length = 401 Score = 25.0 bits (52), Expect = 6.7 Identities = 11/20 (55%), Positives = 16/20 (80%) Frame = +2 Query: 206 EFIKRQDQKRSDLDEQLKEY 265 E +KR+DQK+ D+ E L+EY Sbjct: 272 ELLKRRDQKQQDV-EALQEY 290 >SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces pombe|chr 2|||Manual Length = 857 Score = 24.6 bits (51), Expect = 8.9 Identities = 13/31 (41%), Positives = 14/31 (45%) Frame = +3 Query: 261 NTSTNGANSGPRRRMSSNALKRSRPSARFLV 353 N A P SSN+ K S PS FLV Sbjct: 793 NIPLGHALGNPESNNSSNSFKPSHPSQSFLV 823 >SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA methyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 548 Score = 24.6 bits (51), Expect = 8.9 Identities = 12/40 (30%), Positives = 22/40 (55%) Frame = -3 Query: 289 PLFAPFVDVFLQLFIQVRPLLVLTLDEFWISLTLLFRSGC 170 PL + +D + ++F+Q++ VL + SL + SGC Sbjct: 281 PLLSLSIDFYFRVFVQIKAKPVLVKNLQSQSLLIYHCSGC 320 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,295,120 Number of Sequences: 5004 Number of extensions: 17950 Number of successful extensions: 61 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 61 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 61 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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