Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30104
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g21170.1 68415.m02511 triosephosphate isomerase, chloroplast,... 186 8e-48
At3g55440.1 68416.m06157 triosephosphate isomerase, cytosolic, p... 176 8e-45
At5g55820.1 68418.m06956 expressed protein 31 0.35
At1g25220.1 68414.m03130 anthranilate synthase beta subunit (ASB... 30 0.80
At1g31460.1 68414.m03852 expressed protein 30 1.1
At1g63250.1 68414.m07150 DEAD box RNA helicase, putative similar... 29 1.9
At1g61670.1 68414.m06956 expressed protein similar to membrane p... 29 1.9
At1g64900.1 68414.m07357 cytochrome P450, putative similar to cy... 28 3.2
At4g15380.1 68417.m02350 cytochrome P450 family protein similar ... 28 4.3
At2g12190.1 68415.m01316 cytochrome P450, putative 28 4.3
At1g64950.1 68414.m07362 cytochrome P450, putative similar to cy... 28 4.3
At1g64940.1 68414.m07361 cytochrome P450, putative similar to cy... 28 4.3
At1g24807.1 68414.m03108 anthranilate synthase beta subunit, put... 28 4.3
At5g57890.1 68418.m07242 anthranilate synthase beta subunit, put... 27 5.7
At3g24710.1 68416.m03102 hypothetical protein 27 5.7
At3g07430.1 68416.m00886 YGGT family protein contains Pfam profi... 27 5.7
At5g51380.1 68418.m06370 F-box family protein contains Pfam PF00... 27 7.5
At5g46470.1 68418.m05723 disease resistance protein (TIR-NBS-LRR... 27 7.5
At3g56190.1 68416.m06245 alpha-soluble NSF attachment protein 2 ... 27 7.5
At1g25540.1 68414.m03171 phytochrome and flowering time regulato... 27 7.5
At1g08460.1 68414.m00936 histone deacetylase family protein (HDA... 27 7.5
At4g17090.1 68417.m02575 beta-amylase (CT-BMY) / 1,4-alpha-D-glu... 27 9.9
At3g10370.1 68416.m01243 glycerol-3-phosphate dehydrogenase, put... 27 9.9
At1g25155.1 68414.m03123 anthranilate synthase beta subunit, put... 27 9.9
At1g25083.1 68414.m03118 anthranilate synthase beta subunit, put... 27 9.9
At1g24909.1 68414.m03112 anthranilate synthase beta subunit, put... 27 9.9
>At2g21170.1 68415.m02511 triosephosphate isomerase, chloroplast,
putative similar to Triosephosphate isomerase,
chloroplast precursor: SP|P48496 from Spinacia oleracea,
SP|P46225 from Secale cereale
Length = 315
Score = 186 bits (453), Expect = 8e-48
Identities = 84/160 (52%), Positives = 113/160 (70%)
Frame = +3
Query: 36 KFVVGGNWKMNGDKNQINEIVNNLKKGPLDPNVEVIVGVPAIYLSYVKTIIPDNVEVAAQ 215
KF VGGNWK NG K+ I +++++L L+ +V+V+V P +Y+ VK+ + D ++++ Q
Sbjct: 65 KFFVGGNWKCNGTKDSIAKLISDLNSATLEADVDVVVSPPFVYIDQVKSSLTDRIDISGQ 124
Query: 216 NCWKSPKGAFTGEISPAMIKDVGVNWVILGHSERRTIFGEKDELVAEKVAHALESGLKVI 395
N W GAFTGEIS +KD+G WVILGHSERR + GEKDE + +K A+AL GL VI
Sbjct: 125 NSWVGKGGAFTGEISVEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEGLGVI 184
Query: 396 ACIGETLEERESGKTEEVVFRQLKALVSAIGDKWENIVLA 515
ACIGE LEERE+GKT +V F QLKA A+ W+NIV+A
Sbjct: 185 ACIGEKLEEREAGKTFDVCFAQLKAFADAV-PSWDNIVVA 223
>At3g55440.1 68416.m06157 triosephosphate isomerase, cytosolic,
putative strong similarity to triosephosphate isomerase,
cytosolic from Petunia hybrida [SP|P48495], from Coptis
japonica [SP|P21820]
Length = 254
Score = 176 bits (428), Expect = 8e-45
Identities = 85/165 (51%), Positives = 109/165 (66%), Gaps = 2/165 (1%)
Frame = +3
Query: 27 MGRKFVVGGNWKMNGDKNQINEIVNNLKKG--PLDPNVEVIVGVPAIYLSYVKTIIPDNV 200
M RKF VGGNWK NG ++ +IVN L + P VEV+V P ++L VK+ + +
Sbjct: 1 MARKFFVGGNWKCNGTAEEVKKIVNTLNEAQVPSQDVVEVVVSPPYVFLPLVKSTLRSDF 60
Query: 201 EVAAQNCWKSPKGAFTGEISPAMIKDVGVNWVILGHSERRTIFGEKDELVAEKVAHALES 380
VAAQNCW GAFTGE+S M+ ++ + WVILGHSERR I E E V +KVA+AL
Sbjct: 61 FVAAQNCWVKKGGAFTGEVSAEMLVNLDIPWVILGHSERRAILNESSEFVGDKVAYALAQ 120
Query: 381 GLKVIACIGETLEERESGKTEEVVFRQLKALVSAIGDKWENIVLA 515
GLKVIAC+GETLEERE+G T +VV Q KA+ + W N+V+A
Sbjct: 121 GLKVIACVGETLEEREAGSTMDVVAAQTKAIADRV-TNWSNVVIA 164
>At5g55820.1 68418.m06956 expressed protein
Length = 1826
Score = 31.5 bits (68), Expect = 0.35
Identities = 23/78 (29%), Positives = 39/78 (50%)
Frame = -2
Query: 452 NNFLSLARFSLFESLTNTGNDFQARFKGMSNLFSNKFIFFTKNCSSFRMTKYNPIYSNVF 273
+ +S AR SL E + ND++A G ++ ++K + + S R T P++ + F
Sbjct: 737 HGIISRARSSLIEE--ESANDYKALSDGSNHKSADKQLEVREGNSLLR-TPDRPVFVDNF 793
Query: 272 DHSG*NFTSKSSFWRLPT 219
D N KSS ++PT
Sbjct: 794 DEVPENSREKSSMEKVPT 811
>At1g25220.1 68414.m03130 anthranilate synthase beta subunit (ASB1)
identical to anthranilate synthase beta subunit
GI:403434 from [Arabidopsis thaliana]
Length = 276
Score = 30.3 bits (65), Expect = 0.80
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -2
Query: 215 LGCNFDIIRNDSLHIRQVNSRNTNNYL 135
LGC+F++ RND L + ++ +N L
Sbjct: 95 LGCHFEVYRNDELTVEELKKKNPRGVL 121
>At1g31460.1 68414.m03852 expressed protein
Length = 301
Score = 29.9 bits (64), Expect = 1.1
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = -3
Query: 493 LSPMADTKAFNCLKTTSSVLPDSLSSRVSPIQAMTFKP 380
LS + A NC T S LPD L SR ++++ P
Sbjct: 25 LSDLEQVAAINCSGFTDSTLPDDLESRFRRLKSLPAAP 62
>At1g63250.1 68414.m07150 DEAD box RNA helicase, putative similar to
RNA helicase (RH25) [Arabidopsis thaliana] GI:3776023;
contains Pfam profiles PF00270: DEAD/DEAH box helicase,
PF00271: Helicase conserved C-terminal domain
Length = 798
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/39 (38%), Positives = 22/39 (56%)
Frame = -2
Query: 458 SKNNFLSLARFSLFESLTNTGNDFQARFKGMSNLFSNKF 342
S+ F S++RF ES + G+DF A+ + FS KF
Sbjct: 170 SRRGFSSMSRFKRNESSCDEGDDFDAKKLDTLSPFSPKF 208
>At1g61670.1 68414.m06956 expressed protein similar to membrane
protein PTM1 precursor isolog GI:1931644 from
[Arabidopsis thaliana]
Length = 513
Score = 29.1 bits (62), Expect = 1.9
Identities = 12/46 (26%), Positives = 21/46 (45%), Gaps = 2/46 (4%)
Frame = +3
Query: 138 VIVGVPAIYLSYVKTIIP--DNVEVAAQNCWKSPKGAFTGEISPAM 269
V++ +Y Y P D + + WK+P G G+++P M
Sbjct: 175 VVINKTGMYYLYFMICDPELDGTRIKGRTVWKNPDGYLPGKVAPLM 220
>At1g64900.1 68414.m07357 cytochrome P450, putative similar to
cytochrome p450 GI:438240 from [Solanum melongena]
Length = 506
Score = 28.3 bits (60), Expect = 3.2
Identities = 22/87 (25%), Positives = 40/87 (45%), Gaps = 3/87 (3%)
Frame = +3
Query: 222 WKSPKGAFTGEI-SPAMIKDVGV--NWVILGHSERRTIFGEKDELVAEKVAHALESGLKV 392
W+ + T EI P+ ++ +WV+ ER G ++ +V H L V
Sbjct: 125 WRLLRRNITSEILHPSRVRSYSHARHWVLEILFERFRNHGGEEPIVLIHHLHYAMFALLV 184
Query: 393 IACIGETLEERESGKTEEVVFRQLKAL 473
+ C G+ L+E++ + E + QL +L
Sbjct: 185 LMCFGDKLDEKQIKEVEFIQRLQLLSL 211
>At4g15380.1 68417.m02350 cytochrome P450 family protein similar to
CYTOCHROME P450 93A3 (P450 CP5) (SP:O81973) [Glycine
max]
Length = 517
Score = 27.9 bits (59), Expect = 4.3
Identities = 12/26 (46%), Positives = 17/26 (65%), Gaps = 1/26 (3%)
Frame = -3
Query: 289 FTPTS-LIIAGEISPVKAPFGDFQQF 215
FTPT + AG S + AP+GD+ +F
Sbjct: 112 FTPTDDSLFAGSFSFISAPYGDYWKF 137
>At2g12190.1 68415.m01316 cytochrome P450, putative
Length = 512
Score = 27.9 bits (59), Expect = 4.3
Identities = 15/45 (33%), Positives = 28/45 (62%)
Frame = +3
Query: 330 GEKDELVAEKVAHALESGLKVIACIGETLEERESGKTEEVVFRQL 464
GE+ +V + + +A+ + L V+ C G+ L+E++ + E V RQL
Sbjct: 168 GEEPIVVVDHLHYAMFA-LLVLMCFGDKLDEKQIKQVEYVQRRQL 211
>At1g64950.1 68414.m07362 cytochrome P450, putative similar to
cytochrome P450 89A2 (CYPLXXXIX) (SP:Q42602)
[Arabidopsis thaliana];similar to cytochrome P450
(GI:438242) [Solanum melongena]
Length = 510
Score = 27.9 bits (59), Expect = 4.3
Identities = 15/45 (33%), Positives = 28/45 (62%)
Frame = +3
Query: 330 GEKDELVAEKVAHALESGLKVIACIGETLEERESGKTEEVVFRQL 464
GE+ +V + + +A+ + L V+ C G+ L+E++ + E V RQL
Sbjct: 168 GEEPIVVVDHLHYAMFA-LLVLMCFGDKLDEKQIKQVEYVQRRQL 211
>At1g64940.1 68414.m07361 cytochrome P450, putative similar to
cytochrome p450 GI:438242 from [Solanum melongena]
Length = 511
Score = 27.9 bits (59), Expect = 4.3
Identities = 15/45 (33%), Positives = 28/45 (62%)
Frame = +3
Query: 330 GEKDELVAEKVAHALESGLKVIACIGETLEERESGKTEEVVFRQL 464
GE+ +V + + +A+ + L V+ C G+ L+E++ + E V RQL
Sbjct: 169 GEEPIVVVDHLHYAMFA-LLVLMCFGDKLDEKQIKQVEYVQRRQL 212
>At1g24807.1 68414.m03108 anthranilate synthase beta subunit,
putative similar to anthranilate synthase beta chain
GI:403434; similar to ESTs dbj|AV540153.1,
dbj|AV557490.1, gb|AI997696.1, gb|AW004516.1,
dbj|AV521371.1
Length = 235
Score = 27.9 bits (59), Expect = 4.3
Identities = 12/41 (29%), Positives = 21/41 (51%)
Frame = -2
Query: 257 NFTSKSSFWRLPTVLGCNFDIIRNDSLHIRQVNSRNTNNYL 135
NF + F + L C+F++ RND L + ++ +N L
Sbjct: 40 NFENCYLFLQYMGELQCHFEVYRNDELTVEELKRKNPRGVL 80
>At5g57890.1 68418.m07242 anthranilate synthase beta subunit,
putative strong similarity to anthranilate synthase beta
chain GI:403434 [Arabidopsis thaliana]
Length = 273
Score = 27.5 bits (58), Expect = 5.7
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = -2
Query: 215 LGCNFDIIRNDSLHIRQVNSRNTNNYL 135
LGC+F++ RND L + ++ + L
Sbjct: 92 LGCHFEVYRNDELTVEELKRKKPRGLL 118
>At3g24710.1 68416.m03102 hypothetical protein
Length = 126
Score = 27.5 bits (58), Expect = 5.7
Identities = 17/48 (35%), Positives = 26/48 (54%)
Frame = +3
Query: 339 DELVAEKVAHALESGLKVIACIGETLEERESGKTEEVVFRQLKALVSA 482
D V + E V++ +GE + E E+G ++EV R+LKAL A
Sbjct: 11 DGAVVYVAGSSTEMPSDVMSALGEIVSE-ETGGSKEVASRRLKALEKA 57
>At3g07430.1 68416.m00886 YGGT family protein contains Pfam profile
PF02325: YGGT family (unknown function)
Length = 232
Score = 27.5 bits (58), Expect = 5.7
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = -3
Query: 304 PSITQFTPTSLIIAGEISPVKAPFGDFQQFWAATSTLSG 188
PS++ P S+ I+ SP+ P ++ A +STL+G
Sbjct: 44 PSLSIQNPKSIRISASASPITTPILQTEKSTARSSTLTG 82
>At5g51380.1 68418.m06370 F-box family protein contains Pfam
PF00646: F-box domain; similar to F-box protein FBL2
(GI:6063090) [Homo sapiens]
Length = 479
Score = 27.1 bits (57), Expect = 7.5
Identities = 11/46 (23%), Positives = 24/46 (52%)
Frame = -3
Query: 508 TIFSHLSPMADTKAFNCLKTTSSVLPDSLSSRVSPIQAMTFKPDSR 371
++ H + + +C S + +LSS S ++ +T++PD+R
Sbjct: 411 SVILHWEELESMRVVSCKNIKDSEISAALSSLFSLLKELTWRPDTR 456
>At5g46470.1 68418.m05723 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1127
Score = 27.1 bits (57), Expect = 7.5
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -2
Query: 218 VLGCNFDIIRNDSLHIRQVNSRNTNNYLNIRI 123
VLG DI D LHI + + + +N L ++I
Sbjct: 533 VLGITLDIDETDELHIHESSFKGMHNLLFLKI 564
>At3g56190.1 68416.m06245 alpha-soluble NSF attachment protein 2 /
alpha-SNAP2 / ASNAP2 identical to alpha-soluble NSF
attachment protein 2 / alpha-SNAP2 SP:Q9SPE6 from
[Arabidopsis thaliana]
Length = 289
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/46 (28%), Positives = 26/46 (56%)
Frame = -1
Query: 435 CQILSLRESHQYRQ*LSSQIQGHEQPFQQQVHLFHQKLFFFQNDQV 298
C+I L + +Y + ++ + +Q F+Q + F + FFQN++V
Sbjct: 103 CEIGRLNMAARYYKEIAEYYES-DQKFEQAIAYFEKAAEFFQNEEV 147
>At1g25540.1 68414.m03171 phytochrome and flowering time regulatory
protein (PFT1) PMID: 12815435
Length = 836
Score = 27.1 bits (57), Expect = 7.5
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = -1
Query: 453 KQLPQSCQILSLRESHQYRQ*LSSQIQGHEQPFQQQ 346
+Q+PQ Q + Q +Q SQ+Q H+Q QQQ
Sbjct: 712 QQMPQLQQQQQQHQQQQQQQHQLSQLQHHQQQQQQQ 747
>At1g08460.1 68414.m00936 histone deacetylase family protein (HDA8)
identical to HDA8 [Arabidopsis thaliana] GI:21360988low
similarity to SP|Q9Z2V5 Histone deacetylase 6 (HD6)
(Histone deacetylase mHDA2) {Mus musculus}; contains
Pfam profile PF00850: Histone deacetylase family;
supporting cDNA gi|21360987|gb|AF510167.1|
Length = 377
Score = 27.1 bits (57), Expect = 7.5
Identities = 9/28 (32%), Positives = 20/28 (71%)
Frame = +3
Query: 78 NQINEIVNNLKKGPLDPNVEVIVGVPAI 161
+++ +++ L++GP+ P+V G+PAI
Sbjct: 44 DRVRNMLSILRRGPIAPHVNWFTGLPAI 71
>At4g17090.1 68417.m02575 beta-amylase (CT-BMY) / 1,4-alpha-D-glucan
maltohydrolase identical to beta-amylase enzyme
GI:6065749 from [Arabidopsis thaliana]
Length = 548
Score = 26.6 bits (56), Expect = 9.9
Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 2/64 (3%)
Frame = +3
Query: 27 MGRKFVVGGNWKMNGDKNQINEIVNNLKKGPLDPNV--EVIVGVPAIYLSYVKTIIPDNV 200
M ++ G NW+ Q+ E V N+K+G + E G +Y+ +VK I +NV
Sbjct: 490 MNKRLFEGQNWQ------QLVEFVKNMKEGGHGRRLSKEDTTGSD-LYVGFVKGKIAENV 542
Query: 201 EVAA 212
E AA
Sbjct: 543 EEAA 546
>At3g10370.1 68416.m01243 glycerol-3-phosphate dehydrogenase,
putative similar to glycerol-3-phosphate dehydrogenase
GB:BAA08926 from Mus musculus [SP|Q64521], Homo sapiens
[GI:1020315], Rattus norvegicus [SP|P35571]; contains
Pfam profile PF01266 FAD dependent oxidoreductase
Length = 629
Score = 26.6 bits (56), Expect = 9.9
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +3
Query: 378 SGLKVIACIGETLEERESGKTEEVVFRQLKALVSAIGDKW 497
SG++ +A + T + ES + VVF + LV+ G KW
Sbjct: 401 SGIRPLA-MDPTAKSTESISRDHVVFEENPGLVTITGGKW 439
>At1g25155.1 68414.m03123 anthranilate synthase beta subunit,
putative strong similarity to anthranilate synthase beta
subunit GI:403434 from (Arabidopsis thaliana)
Length = 222
Score = 26.6 bits (56), Expect = 9.9
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = -2
Query: 215 LGCNFDIIRNDSLHIRQVNSRNTNNYL 135
L C+F++ RND L + ++ +N L
Sbjct: 41 LQCHFEVYRNDELTVEELKRKNPRGVL 67
>At1g25083.1 68414.m03118 anthranilate synthase beta subunit,
putative strong similarity to anthranilate synthase beta
subunit GI:403434 from (Arabidopsis thaliana); similar
to ESTs dbj|AV540153.1, dbj|AV557490.1, gb|AI997696.1,
gb|AW004516.1, dbj|AV521371.1
Length = 222
Score = 26.6 bits (56), Expect = 9.9
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = -2
Query: 215 LGCNFDIIRNDSLHIRQVNSRNTNNYL 135
L C+F++ RND L + ++ +N L
Sbjct: 41 LQCHFEVYRNDELTVEELKRKNPRGVL 67
>At1g24909.1 68414.m03112 anthranilate synthase beta subunit,
putative strong similarity to anthranilate synthase beta
subunit GI:403434 from (Arabidopsis thaliana)
Length = 222
Score = 26.6 bits (56), Expect = 9.9
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = -2
Query: 215 LGCNFDIIRNDSLHIRQVNSRNTNNYL 135
L C+F++ RND L + ++ +N L
Sbjct: 41 LQCHFEVYRNDELTVEELKRKNPRGVL 67
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,829,186
Number of Sequences: 28952
Number of extensions: 217279
Number of successful extensions: 643
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 625
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 642
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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