Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30102
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 165 1e-41
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 162 1e-40
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 160 4e-40
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 159 1e-39
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 93 8e-20
At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes... 29 1.9
At2g33420.1 68415.m04096 expressed protein 29 2.5
At5g45610.1 68418.m05605 expressed protein 28 3.2
At3g23270.1 68416.m02933 regulator of chromosome condensation (R... 27 5.7
At5g49430.1 68418.m06116 transducin family protein / WD-40 repea... 27 7.5
At1g13770.1 68414.m01616 expressed protein contains Pfam domain,... 27 7.5
At3g53320.1 68416.m05883 expressed protein 27 9.9
At3g02750.1 68416.m00267 protein phosphatase 2C family protein /... 27 9.9
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 165 bits (402), Expect = 1e-41
Identities = 89/158 (56%), Positives = 105/158 (66%), Gaps = 1/158 (0%)
Frame = +2
Query: 41 AFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDK-DRLTWDIHFWLG 217
AF AG+KPG EIWRIENFE V VPK++ G FY GD+YIVL TT +K +DIHFW+G
Sbjct: 9 AFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIG 68
Query: 218 SRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYXSKEFLEYFSPAIRYLKGGHASGF 397
TSQDEAG AA+ TV L D G AVQHRE+Q + S +FL YF P I L+GG ASGF
Sbjct: 69 KDTSQDEAGTAAVKTVEL-DAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLEGGVASGF 127
Query: 398 XHVTINEGTXKRLFXIKGKRNVRVKQVKPTFESLNNGD 511
V E RL+ KGKR +R+KQV SLN+ D
Sbjct: 128 KTVE-EEVFETRLYTCKGKRAIRLKQVPFARSSLNHDD 164
Score = 54.0 bits (124), Expect = 6e-08
Identities = 33/110 (30%), Positives = 51/110 (46%), Gaps = 1/110 (0%)
Frame = +2
Query: 71 LEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRL-TWDIHFWLGSRTSQDEAGA 247
LE+W + +PK G YSGD Y+VL T +R + + W G ++ ++
Sbjct: 392 LEVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGERKDEYFLSCWFGKKSIPEDQD- 450
Query: 248 AAILTVNLDDEQFQGSAVQHREVQYYXSKEFLEYFSPAIRYLKGGHASGF 397
AI N +G VQ R + +F+ F P + LKGG +SG+
Sbjct: 451 TAIRLANTMSNSLKGRPVQGRIYEGKEPPQFVALFQPMV-VLKGGLSSGY 499
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 162 bits (393), Expect = 1e-40
Identities = 87/158 (55%), Positives = 103/158 (65%), Gaps = 1/158 (0%)
Frame = +2
Query: 41 AFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDK-DRLTWDIHFWLG 217
AF G+KPG EIWRIENFEPV VPK++ G FY GD+YIVL TT +K +DIHFW+G
Sbjct: 11 AFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIG 70
Query: 218 SRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYXSKEFLEYFSPAIRYLKGGHASGF 397
TSQDEAG AA+ TV L D G AVQ+RE+Q + S +FL YF P I L+GG ASGF
Sbjct: 71 KDTSQDEAGTAAVKTVEL-DAALGGRAVQYREIQGHESDKFLSYFKPCIIPLEGGVASGF 129
Query: 398 XHVTINEGTXKRLFXIKGKRNVRVKQVKPTFESLNNGD 511
E RL+ KGKR V +KQV SLN+ D
Sbjct: 130 KKPE-EEEFETRLYTCKGKRAVHLKQVPFARSSLNHDD 166
Score = 54.4 bits (125), Expect = 4e-08
Identities = 44/159 (27%), Positives = 72/159 (45%), Gaps = 11/159 (6%)
Frame = +2
Query: 71 LEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRL-TWDIHFWLGSRTSQDEAGA 247
LE+W I+ + K G YSGD Y+VL T +R + + W G ++Q++
Sbjct: 394 LEVWYIDANSKTVLSKDHVGKLYSGDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQET 453
Query: 248 AAILTVNLDDEQFQGSAVQHREVQYYXSKEFLEYFSPAIRYLKGGHASGFXHVTINEG-- 421
A L + + +G VQ R + +F+ F + LKGG +SG+ + +G
Sbjct: 454 AVRLASTMTN-SLKGRPVQARIFEGKEPPQFVALFQHMV-VLKGGLSSGYKNSMTEKGSS 511
Query: 422 ----TXKRLFXIK----GKRNVRVKQVKPTFESLNNGDC 514
T + + I+ G N + QV+ SLN+ DC
Sbjct: 512 GETYTPESIALIQVSGTGVHNNKALQVEAVATSLNSYDC 550
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 160 bits (389), Expect = 4e-40
Identities = 85/164 (51%), Positives = 108/164 (65%), Gaps = 1/164 (0%)
Frame = +2
Query: 23 MPEVHQAFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKD-RLTWD 199
M ++ QA AG+K G+EIWRIENF+PV VP+ G F++GDSYIVL TT + L D
Sbjct: 5 MRDLDQALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHD 64
Query: 200 IHFWLGSRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYXSKEFLEYFSPAIRYLKG 379
IH+WLG +SQDEAGA A++TV L D G AVQ+REVQ + +++FL YF P I +G
Sbjct: 65 IHYWLGKDSSQDEAGAVAVMTVEL-DSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEG 123
Query: 380 GHASGFXHVTINEGTXKRLFXIKGKRNVRVKQVKPTFESLNNGD 511
G ASGF HV E RL+ KGK VRVK+V +LN+ D
Sbjct: 124 GVASGFNHVKPEE-HQTRLYICKGKHVVRVKEVPFVRSTLNHED 166
Score = 44.0 bits (99), Expect = 6e-05
Identities = 35/156 (22%), Positives = 67/156 (42%), Gaps = 8/156 (5%)
Frame = +2
Query: 71 LEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRLTWDIHFWLGSRT-SQDEAGA 247
L++WRI E + + + FYSGD YI+ + +DR + W G ++ +D A A
Sbjct: 396 LQVWRINCEEKILLEAAEQSKFYSGDCYILQYSYPGEDREEHLVGTWFGKQSVEEDRASA 455
Query: 248 AAILTVNLDDEQFQGSAVQHRE----VQYY-XSKEFLEYFSPAIRYLKGGHASGFXHVTI 412
++ ++ +F + + E +Q++ + F+ + K A T
Sbjct: 456 ISLANKMVESMKFVPAQARINEGKEPIQFFVIMQSFITFKGGVSDAFKKYIAENDIPDTT 515
Query: 413 NEGTXKRLFXIKGK--RNVRVKQVKPTFESLNNGDC 514
E LF ++G N++ Q++ LN+ C
Sbjct: 516 YEAEGVALFRVQGSGPENMQAIQIEAASAGLNSSHC 551
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 159 bits (386), Expect = 1e-39
Identities = 86/164 (52%), Positives = 106/164 (64%), Gaps = 1/164 (0%)
Frame = +2
Query: 23 MPEVHQAFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDK-DRLTWD 199
M ++ AF AG+K G+EIWRIENF P +PK+ G F++GDSYIVL TT K L D
Sbjct: 5 MRDLDPAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGALRHD 64
Query: 200 IHFWLGSRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYXSKEFLEYFSPAIRYLKG 379
IH+WLG TSQDEAG AA+ TV L D G AVQ+REVQ + +++FL YF P I +G
Sbjct: 65 IHYWLGKDTSQDEAGTAAVKTVEL-DAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEG 123
Query: 380 GHASGFXHVTINEGTXKRLFXIKGKRNVRVKQVKPTFESLNNGD 511
G ASGF HV + E RLF +GK V VK+V SLN+ D
Sbjct: 124 GVASGFKHV-VAEEHITRLFVCRGKHVVHVKEVPFARSSLNHDD 166
Score = 47.2 bits (107), Expect = 7e-06
Identities = 41/171 (23%), Positives = 74/171 (43%), Gaps = 12/171 (7%)
Frame = +2
Query: 29 EVHQAFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRLTWDIHF 208
E Q F D L++WR+ + FYSGD Y+ + +++ I
Sbjct: 382 EEPQVFIDC--TGNLQVWRVNGQAKTLLQAADHSKFYSGDCYVFQYSYPGEEKEEVLIGT 439
Query: 209 WLGSRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYXSKEFLEYF--SPAIRYLKGG 382
W G ++ ++E G+A V++ + + + + Y KE +++F + KGG
Sbjct: 440 WFGKQSVEEERGSA----VSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVFKGG 495
Query: 383 HASGF----XHVTINEGTXKR----LFXIKGK--RNVRVKQVKPTFESLNN 505
+SG+ +++ T LF I+G N++ QV P SLN+
Sbjct: 496 ISSGYKKYIAEKEVDDDTYNENGVALFRIQGSGPENMQAIQVDPVAASLNS 546
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 93.5 bits (222), Expect = 8e-20
Identities = 57/162 (35%), Positives = 89/162 (54%), Gaps = 1/162 (0%)
Frame = +2
Query: 29 EVHQAFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDK-DRLTWDIH 205
++ AF G K GLEIW + N + +++PK+ FG F+SG++Y+VL T K + +DIH
Sbjct: 7 DIDSAFQGVGTKSGLEIWCVYNKQLISIPKSSFGKFHSGNAYLVLRTFLRKIESPQYDIH 66
Query: 206 FWLGSRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYXSKEFLEYFSPAIRYLKGGH 385
+WLG ++ ++ A+ ++L D VQ+REVQ +++FL YF P I ++G +
Sbjct: 67 YWLGIDANEVDSILASDKALDL-DAALGCCTVQYREVQGQETEKFLSYFKPCIIPVEGKY 125
Query: 386 ASGFXHVTINEGTXKRLFXIKGKRNVRVKQVKPTFESLNNGD 511
+ E L KG VRVK+V SLN+ D
Sbjct: 126 SPKTG--IAGETYQVTLLRCKGDHVVRVKEVPFLRSSLNHDD 165
Score = 29.9 bits (64), Expect = 1.1
Identities = 23/107 (21%), Positives = 44/107 (41%)
Frame = +2
Query: 62 KPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRLTWDIHFWLGSRTSQDEA 241
+ L++WR++ + + ++GD Y+V K+R ++ W+G + Q +
Sbjct: 388 RDNLKVWRVDGDDVSLLSIPDQTKLFTGDCYLVQYKYTYKERTEHLLYVWIGCESIQQDR 447
Query: 242 GAAAILTVNLDDEQFQGSAVQHREVQYYXSKEFLEYFSPAIRYLKGG 382
A AI + +G +V Q F F + + KGG
Sbjct: 448 -ADAITNASAIVGTTKGESVLCHIYQGNEPSRFFPMFQSLVVF-KGG 492
>At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes
from this gene
Length = 1035
Score = 29.1 bits (62), Expect = 1.9
Identities = 25/86 (29%), Positives = 38/86 (44%)
Frame = +3
Query: 63 SRVSKYGGLRTSNRSPFRRLSSVSSTPGTPTLY*TPQAIRTV*HGTSISGSARGPARTKP 242
SRV + GL+ RSP RR+S+V + G + +P ++ G+ G P
Sbjct: 107 SRVKRALGLKMLKRSPSRRMSTVGTVVGAVSAPSSPGNNGSIGSGSGHFSPGAGFFTVPP 166
Query: 243 ARRPSSR*TWTTNNSRDQRYSTERSN 320
AR R + R Q TE+S+
Sbjct: 167 AR--PRRPLTSAEIMRQQMKVTEQSD 190
>At2g33420.1 68415.m04096 expressed protein
Length = 1039
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = +3
Query: 63 SRVSKYGGLRTSNRSPFRRLSSVSSTPGTPT 155
SRV + GL+ RSP RR+S++ + G T
Sbjct: 113 SRVKRALGLKMLKRSPSRRMSTIGAAGGAAT 143
>At5g45610.1 68418.m05605 expressed protein
Length = 633
Score = 28.3 bits (60), Expect = 3.2
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = -3
Query: 139 VEETELSLRNGDRFEVLNPPYFETRLPA 56
++ E +L+ D + L PPY T LPA
Sbjct: 16 IDSIETTLKKADMYRPLPPPYLPTFLPA 43
>At3g23270.1 68416.m02933 regulator of chromosome condensation
(RCC1) family protein contains Pfam domain PF00415:
Regulator of chromosome condensation (RCC1); similar to
zinc finger protein (GI:15811367) [Arabidopsis
thaliana]; similar to chromosome condensation regulator
protein (GI:22770461) [Cicer arietinum]
Length = 1045
Score = 27.5 bits (58), Expect = 5.7
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = -1
Query: 105 TGSKFSILHISRPGFLPASANA 40
+ +K S++ SRPGF P S+NA
Sbjct: 701 SANKNSVMSSSRPGFTPESSNA 722
>At5g49430.1 68418.m06116 transducin family protein / WD-40 repeat
family protein similar to WD-repeat protein 9
(SP:Q9NSI6) {Homo sapiens}; contains Pfam PF00400: WD
domain, G-beta repeat (4 copies)
Length = 1677
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/33 (39%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
Frame = -1
Query: 114 GTATGSKFSILHISRPGFLPASANAW-CTSGIL 19
G A GSKFS+ S+ +P N+W C I+
Sbjct: 457 GCAAGSKFSVTDYSKDENVPKFKNSWFCHDNIV 489
>At1g13770.1 68414.m01616 expressed protein contains Pfam domain,
PF04884: Protein of unknown function, DUF647; contains
non-consensus (GC) splice site at intron 2
Length = 440
Score = 27.1 bits (57), Expect = 7.5
Identities = 18/46 (39%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -1
Query: 300 TADPWNCSSSKFTVRMAAAPASSWLVLEPSQKWMSHV-RRSLSPVV 166
T + WN SSS + A ASS L ++ S +HV RR L V
Sbjct: 13 TLEEWNGSSSTKLFKTATITASSSLSIQRSANRFNHVWRRVLQAFV 58
>At3g53320.1 68416.m05883 expressed protein
Length = 553
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/48 (31%), Positives = 28/48 (58%)
Frame = +3
Query: 192 HGTSISGSARGPARTKPARRPSSR*TWTTNNSRDQRYSTERSNITSPR 335
HG+S S S + +T+PAR P +TN+ + S++ +++SP+
Sbjct: 494 HGSSSSASKKSGGKTQPARSPIQE---STNSK--SKASSKVVSVSSPK 536
>At3g02750.1 68416.m00267 protein phosphatase 2C family protein /
PP2C family protein similar to protein phosphatase-2C;
PP2C (GI:3643088) [Mesembryanthemum crystallinum];
contains Pfam PF00481 : Protein phosphatase 2C domain;
Length = 492
Score = 26.6 bits (56), Expect = 9.9
Identities = 24/84 (28%), Positives = 38/84 (45%), Gaps = 7/84 (8%)
Frame = +2
Query: 26 PEVHQAFADAGRKPGLEIWR------IENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDR 187
PEV + + PGL + R +++F ++VP F D +IVL T G D
Sbjct: 284 PEVCRVWLPNCDSPGLAMARAFGDFCLKDFGLISVPDVSFRQLTEKDEFIVLATDGIWDV 343
Query: 188 LT-WDIHFWLGSRTSQDEAGAAAI 256
L+ D+ + S S+ A A +
Sbjct: 344 LSNEDVVAIVASAPSRSSAARALV 367
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,917,865
Number of Sequences: 28952
Number of extensions: 214665
Number of successful extensions: 623
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 596
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 608
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -