Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30100
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 73 2e-14
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 72 4e-14
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 49 5e-07
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 37 0.002
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 32 0.044
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 26 3.8
SPAC683.03 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 25 8.9
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 73.3 bits (172), Expect = 2e-14
Identities = 44/139 (31%), Positives = 69/139 (49%), Gaps = 3/139 (2%)
Frame = +2
Query: 95 QQRPAPAGDRQSSR-GSRKAXRTGSNXFSMXSXKXVAXFKEAFQLMDHDXDGIIGKNDLR 271
++ P + S R ++ A R S F+ + + KEAF L+D D DG IG+ D++
Sbjct: 12 KRAPFSSNTTSSQRVAAQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVK 71
Query: 272 ATFDSLGRLASDKELDXMVXEASGPINFTQLLTLFANRMSGGSDEXDVXINAFXTFD--E 445
SL + AS+ ++ M + PIN LT + + S D+ + AF TFD +
Sbjct: 72 TMLTSLNQDASEDSINHMFESINPPINLAAFLTAMGSMLCRISPRNDL-LEAFSTFDDTQ 130
Query: 446 EGKIDSERLRHALMTWGDK 502
GKI +R AL + GD+
Sbjct: 131 SGKIPISTMRDALSSMGDR 149
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 72.1 bits (169), Expect = 4e-14
Identities = 42/112 (37%), Positives = 61/112 (54%), Gaps = 6/112 (5%)
Frame = +2
Query: 197 VAXFKEAFQLMDHDXDGIIGKNDLRATFDSLGRLASDKELDXMVXE----ASGPINFTQL 364
+A F+EAF L D D DG I N+L SLG+ + EL M+ E +G I+FT+
Sbjct: 11 IAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEF 70
Query: 365 LTLFANRMSGGSDEXDVXINAFXTFDEEGK--IDSERLRHALMTWGDKFSAD 514
LT+ A +M +E +V AF FD++G I E L H L + G++ S +
Sbjct: 71 LTMMARKMKDTDNEEEVR-EAFKVFDKDGNGYITVEELTHVLTSLGERLSQE 121
Score = 34.3 bits (75), Expect = 0.011
Identities = 19/63 (30%), Positives = 33/63 (52%), Gaps = 4/63 (6%)
Frame = +2
Query: 209 KEAFQLMDHDXDGIIGKNDLRATFDSLGRLASDKELDXMVXEA----SGPINFTQLLTLF 376
+EAF++ D D +G I +L SLG S +E+ M+ EA G IN+ + +
Sbjct: 88 REAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVI 147
Query: 377 ANR 385
+++
Sbjct: 148 SSK 150
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 48.8 bits (111), Expect = 5e-07
Identities = 31/102 (30%), Positives = 52/102 (50%), Gaps = 2/102 (1%)
Frame = +2
Query: 209 KEAFQLMDHDXDGIIGKNDLRATFDSLGRLASDKELDXMVXEASGPINFTQLLTLFANRM 388
KEAF L D D DG+I + + + SLG +D EL + E I+ + ++ +N++
Sbjct: 12 KEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVSNKL 71
Query: 389 SGGSDEXDVXINAFXTFDEE--GKIDSERLRHALMTWGDKFS 508
E + I AF FD++ G I++ + + T G+K S
Sbjct: 72 RETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLGEKLS 112
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 36.7 bits (81), Expect = 0.002
Identities = 16/41 (39%), Positives = 25/41 (60%)
Frame = +2
Query: 206 FKEAFQLMDHDXDGIIGKNDLRATFDSLGRLASDKELDXMV 328
F + FQ+ D D G+IG +LR SLG S++E+D ++
Sbjct: 79 FVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 32.3 bits (70), Expect = 0.044
Identities = 27/94 (28%), Positives = 41/94 (43%), Gaps = 6/94 (6%)
Frame = +2
Query: 212 EAFQLMDHDXDGIIGKNDLRATFDSLGRLASDKELDXMVXE----ASGPINFTQLLTLFA 379
EAF+L D D D I ++LRA +LG A E+ ++ + G + + +
Sbjct: 41 EAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMT 100
Query: 380 NRMSGGSDEXDVXINAFXTF--DEEGKIDSERLR 475
++ D + AF F DE GKI LR
Sbjct: 101 EKIV-ERDPLEEIKRAFELFDDDETGKISLRNLR 133
Score = 32.3 bits (70), Expect = 0.044
Identities = 16/42 (38%), Positives = 21/42 (50%)
Frame = +2
Query: 209 KEAFQLMDHDXDGIIGKNDLRATFDSLGRLASDKELDXMVXE 334
K AF+L D D G I +LR L D+EL+ M+ E
Sbjct: 113 KRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEE 154
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 25.8 bits (54), Expect = 3.8
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = +2
Query: 209 KEAFQLMDHDXDGIIGKNDLRATFDSLGRLASDKELDXMV 328
+EAF L D G I DLR + LG + ++L M+
Sbjct: 15 EEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54
>SPAC683.03 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 105
Score = 24.6 bits (51), Expect = 8.9
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = -1
Query: 111 GAGRCWGLFG 82
G+G+CWGL G
Sbjct: 20 GSGKCWGLLG 29
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,447,386
Number of Sequences: 5004
Number of extensions: 20314
Number of successful extensions: 43
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 41
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 43
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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