BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30087 (810 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 78 1e-15 SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 65 1e-11 SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 38 0.001 SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos... 27 4.2 SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.5 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 26 5.5 SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces po... 26 5.5 SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi... 26 7.3 SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase Su... 26 7.3 SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|S... 26 7.3 SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr 1|... 25 9.6 >SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein Stg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 174 Score = 78.2 bits (184), Expect = 1e-15 Identities = 55/146 (37%), Positives = 77/146 (52%), Gaps = 4/146 (2%) Frame = +3 Query: 63 EQEVLTWISDVLGEPLPNGAYE--DVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN 236 E+E WI + L L N + D L+ GVILC++ K A G+ + +E F MEN Sbjct: 6 EKEAREWIEETLHTKL-NAQLDLLDQLQSGVILCRIC-KEALGANIRYKESNMPFVQMEN 63 Query: 237 IQRF-QAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYALGRITQKHPEFTGPQLGPKM 413 I F A + VP +++FQT+DLFERRN QV +++ R K LGPK+ Sbjct: 64 ISAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKL 123 Query: 414 ADKNERTFTEEQLRAHNAELN-LQMG 488 A+K R F+ +Q R +N LQ G Sbjct: 124 AEKKPRVFSAQQQREFREGVNSLQYG 149 >SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual Length = 1489 Score = 65.3 bits (152), Expect = 1e-11 Identities = 35/98 (35%), Positives = 52/98 (53%), Gaps = 2/98 (2%) Frame = +3 Query: 69 EVLTWISDVLGEPL-PNGAYEDVLRDGVILCKLANKLAPGSVKKI-QERGTNFQLMENIQ 242 E WI + LG L P +E LR+GV+L L K P + KI F+ +NI Sbjct: 46 EAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNIN 105 Query: 243 RFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 356 +F I G+PE F+ D++E +N+P+V C++AL Sbjct: 106 KFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143 >SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 962 Score = 38.3 bits (85), Expect = 0.001 Identities = 30/101 (29%), Positives = 45/101 (44%), Gaps = 5/101 (4%) Frame = +3 Query: 69 EVLTWISDVLGEPLPN-GAYEDVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN--- 236 E W+ + N + D L +G +LC+LA K P + R +Q+ E Sbjct: 69 EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYPKLASNWKPR---YQISERNTV 125 Query: 237 -IQRFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 356 + F + G+ F+T DL R NIP+V CL+AL Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166 >SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 26.6 bits (56), Expect = 4.2 Identities = 14/41 (34%), Positives = 22/41 (53%) Frame = -3 Query: 232 SMSWKLVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFG 110 S+++KL + DP LAN +++ SLS +Y P G Sbjct: 33 SLAYKLYSMLRSLKLDPHFMELANPKKVDSSLSYENYQPIG 73 >SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 163 Score = 26.2 bits (55), Expect = 5.5 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +2 Query: 149 DPLQIGQQISSWISEKNPRERNQLPAHG 232 D LQ+ ++ SWI+EK E +L + G Sbjct: 11 DSLQVDEKAKSWIAEKVEEELRRLESVG 38 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 26.2 bits (55), Expect = 5.5 Identities = 10/19 (52%), Positives = 14/19 (73%) Frame = -3 Query: 601 FTNTDINVENIGKNLEAIY 545 F N VEN+GK++EA+Y Sbjct: 1855 FDNYSEKVENLGKSIEALY 1873 >SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces pombe|chr 3|||Manual Length = 452 Score = 26.2 bits (55), Expect = 5.5 Identities = 17/64 (26%), Positives = 27/64 (42%) Frame = -3 Query: 217 LVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFGSGSPKTSEIQVRTSCSWSLFLAGI 38 L+ ++W +P + +Q++ PS+ T A P E + FL GI Sbjct: 257 LMHMAWALLDNPNLFVEPYVQQLMPSILTCLVAKRLGSDPNNHEHYALRDL--AAFLLGI 314 Query: 37 VCRR 26 VC R Sbjct: 315 VCDR 318 >SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schizosaccharomyces pombe|chr 2|||Manual Length = 749 Score = 25.8 bits (54), Expect = 7.3 Identities = 11/30 (36%), Positives = 15/30 (50%) Frame = -3 Query: 169 LANLQRITPSLSTSSYAPFGSGSPKTSEIQ 80 L+N +TPS S S Y P +T +Q Sbjct: 689 LSNFNSVTPSSSASLYPPLIPSEARTPSVQ 718 >SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase Sum3|Schizosaccharomyces pombe|chr 3|||Manual Length = 636 Score = 25.8 bits (54), Expect = 7.3 Identities = 14/52 (26%), Positives = 21/52 (40%) Frame = +3 Query: 372 KHPEFTGPQLGPKMADKNERTFTEEQLRAHNAELNLQMGFNKGASQSGHGGF 527 + F G G + + + R R N G++ G S SG+GGF Sbjct: 565 RESSFGGNGRGGRYSGRGGRGGNAYGARDFRRPTNSSSGYSSGPSYSGYGGF 616 >SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|Schizosaccharomyces pombe|chr 2|||Manual Length = 514 Score = 25.8 bits (54), Expect = 7.3 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = +1 Query: 481 KWASIRAPPSPVMVASVTPATCKLLLNFSQYF 576 ++ IR+PP+ V + S+TP NF Q + Sbjct: 46 RYTKIRSPPTRVSIESITPKQRFPAPNFEQAY 77 >SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr 1|||Manual Length = 623 Score = 25.4 bits (53), Expect = 9.6 Identities = 8/18 (44%), Positives = 11/18 (61%) Frame = -3 Query: 343 HNVTWGMLRLSKRSAVWN 290 HN WG R + +A+WN Sbjct: 491 HNFVWGRFRELEHAAMWN 508 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,547,817 Number of Sequences: 5004 Number of extensions: 81730 Number of successful extensions: 251 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 238 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 250 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 394431430 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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