Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30087
(810 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g47500.1 68415.m05929 kinesin motor protein-related 50 1e-06
At5g27000.1 68418.m03221 kinesin motor protein-related non-conse... 49 4e-06
At5g41310.1 68418.m05020 kinesin motor protein-related 44 9e-05
At1g63640.2 68414.m07198 kinesin motor protein-related C-termina... 43 2e-04
At1g63640.1 68414.m07197 kinesin motor protein-related C-termina... 43 2e-04
At3g10310.1 68416.m01237 kinesin motor protein-related similar t... 42 4e-04
At1g09170.1 68414.m01024 kinesin motor protein-related similar t... 41 8e-04
At3g44730.1 68416.m04814 kinesin motor protein-related similar t... 36 0.042
At5g35700.1 68418.m04269 fimbrin-like protein, putative similar ... 35 0.073
At5g48460.1 68418.m05992 fimbrin-like protein, putative strong s... 34 0.097
At4g26700.1 68417.m03848 fimbrin-like protein (FIM1) identical t... 34 0.13
At5g55400.1 68418.m06902 fimbrin-like protein, putative similar ... 33 0.17
At2g04750.1 68415.m00485 fimbrin-like protein, putative similar ... 33 0.30
At5g62240.1 68418.m07815 expressed protein various predicted pro... 31 0.68
At5g25475.2 68418.m03031 expressed protein 31 0.90
At5g25475.1 68418.m03030 expressed protein 31 0.90
At3g59100.1 68416.m06589 glycosyl transferase family 48 protein ... 31 0.90
At3g11170.1 68416.m01355 omega-3 fatty acid desaturase, chloropl... 30 2.1
At5g25470.2 68418.m03029 expressed protein 29 2.8
At5g25470.1 68418.m03028 expressed protein 29 2.8
At2g17010.1 68415.m01961 mechanosensitive ion channel domain-con... 29 2.8
At2g25730.1 68415.m03084 expressed protein 29 3.7
At3g07070.1 68416.m00840 protein kinase family protein contains ... 29 4.8
At2g46510.1 68415.m05796 basic helix-loop-helix (bHLH) family pr... 29 4.8
At2g31010.1 68415.m03781 protein kinase family protein contains ... 29 4.8
At2g45600.1 68415.m05670 expressed protein low similarity to PrM... 28 6.4
>At2g47500.1 68415.m05929 kinesin motor protein-related
Length = 974
Score = 50.4 bits (115), Expect = 1e-06
Identities = 34/101 (33%), Positives = 51/101 (50%), Gaps = 12/101 (11%)
Frame = +3
Query: 90 DVLGEPLPNGAYEDVLRDGVILCKLANKLAPGSVKKIQERGTN------------FQLME 233
D+ EP G LR G+ILCK+ NK+ PG+V K+ E + FQ E
Sbjct: 65 DLPAEPTEEGLRLG-LRSGIILCKVLNKVQPGAVSKVVESPCDAILVADGAPLSAFQYFE 123
Query: 234 NIQRFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 356
N++ F AI++ G P F+ +DL + N +V C+ A+
Sbjct: 124 NVRNFLVAIQEMGFP---TFEASDLEQGGNASRVVNCVLAI 161
>At5g27000.1 68418.m03221 kinesin motor protein-related
non-consensus AT donor splice site at exon 12;
non-consensus AC acceptor splice site at exon 13
Length = 987
Score = 48.8 bits (111), Expect = 4e-06
Identities = 34/113 (30%), Positives = 56/113 (49%), Gaps = 17/113 (15%)
Frame = +3
Query: 69 EVLTWISDVLG----EPLPNGAYEDV----LRDGVILCKLANKLAPGSVKKIQERGTN-- 218
E W+ D++G + P E+ LR G++LC + NK+ PGSV K+ E +
Sbjct: 49 EAAGWLRDMIGVSNGKDFPGEPSEEEFRLGLRSGIVLCNVLNKVNPGSVSKVVEAPDDVA 108
Query: 219 -------FQLMENIQRFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 356
FQ ENI+ F AI++ G+P F+ +D+ + ++ C+ AL
Sbjct: 109 DGAALSAFQYFENIRNFLVAIEEMGLPS---FEASDMEKGGKSIRIVNCILAL 158
>At5g41310.1 68418.m05020 kinesin motor protein-related
Length = 961
Score = 44.4 bits (100), Expect = 9e-05
Identities = 31/85 (36%), Positives = 48/85 (56%), Gaps = 6/85 (7%)
Frame = +3
Query: 51 NKDQEQEVLTWISDVLGE-PLPNGAYEDVLR----DGVILCKLANKLAPGSVKKIQERGT 215
NK Q ++ W+++ L LP A E+ LR DG +LC L N+L+PGS++ G
Sbjct: 39 NKQGHQSLVEWLNETLPYLNLPWEASEEELRACLVDGTVLCNLLNQLSPGSMR----MGG 94
Query: 216 NFQL-MENIQRFQAAIKKYGVPEEE 287
+F+ NI+RF AA+ + +P E
Sbjct: 95 SFEPGCVNIERFLAAMDEMTLPRFE 119
>At1g63640.2 68414.m07198 kinesin motor protein-related C-terminal
region is similar to C-term region of kinesin motor
protein GB:AAB51397 (Mus musculus); contains Pfam
profile: PF00225 Kinesin motor domain
Length = 1065
Score = 43.2 bits (97), Expect = 2e-04
Identities = 29/86 (33%), Positives = 47/86 (54%), Gaps = 6/86 (6%)
Frame = +3
Query: 51 NKDQEQEVLTWISDVLGE-PLPNGAYED----VLRDGVILCKLANKLAPGSVKKIQERGT 215
+K Q ++ W+++ L LP A ED LRDG +LC L N+L+PGS++ G
Sbjct: 38 SKKGHQSLVEWLNETLPYLKLPWEASEDELRACLRDGTVLCSLLNQLSPGSMR----MGG 93
Query: 216 NFQ-LMENIQRFQAAIKKYGVPEEEI 290
+F+ I+RF A+ + +P E+
Sbjct: 94 SFEPASVKIERFLTAMDEMALPRFEV 119
>At1g63640.1 68414.m07197 kinesin motor protein-related C-terminal
region is similar to C-term region of kinesin motor
protein GB:AAB51397 (Mus musculus); contains Pfam
profile: PF00225 Kinesin motor domain
Length = 1064
Score = 43.2 bits (97), Expect = 2e-04
Identities = 29/86 (33%), Positives = 47/86 (54%), Gaps = 6/86 (6%)
Frame = +3
Query: 51 NKDQEQEVLTWISDVLGE-PLPNGAYED----VLRDGVILCKLANKLAPGSVKKIQERGT 215
+K Q ++ W+++ L LP A ED LRDG +LC L N+L+PGS++ G
Sbjct: 38 SKKGHQSLVEWLNETLPYLKLPWEASEDELRACLRDGTVLCSLLNQLSPGSMR----MGG 93
Query: 216 NFQ-LMENIQRFQAAIKKYGVPEEEI 290
+F+ I+RF A+ + +P E+
Sbjct: 94 SFEPASVKIERFLTAMDEMALPRFEV 119
>At3g10310.1 68416.m01237 kinesin motor protein-related similar to
carboxy-terminal kinesin 2 GB:P79955 [Xenopus laevis]
Length = 897
Score = 42.3 bits (95), Expect = 4e-04
Identities = 31/112 (27%), Positives = 53/112 (47%), Gaps = 16/112 (14%)
Frame = +3
Query: 69 EVLTWISDVLGE-PLPNGAYE----DVLRDGVILCKLANKLAPGSVKKIQERGT------ 215
+ + W+ V+G+ +PN E LR+G+ILC NK+ PG+V K+ E +
Sbjct: 24 QAVQWLKSVVGQLGIPNQPSEKEFISCLRNGMILCNAINKIHPGAVSKVVENYSYLNGEY 83
Query: 216 ----NFQLMENIQRFQAAIKKYGVPEEEIFQ-TADLFERRNIPQVTLCLYAL 356
+Q EN++ F A++ +P E D E ++ +V C+ L
Sbjct: 84 QLPPAYQYFENVRNFLVALETLRLPGFEASDLEKDNLESGSVTKVVDCILGL 135
>At1g09170.1 68414.m01024 kinesin motor protein-related similar to
GB:AAB61066
Length = 1010
Score = 41.1 bits (92), Expect = 8e-04
Identities = 30/116 (25%), Positives = 53/116 (45%), Gaps = 20/116 (17%)
Frame = +3
Query: 69 EVLTWISDVLG----EPLPNGAYED----VLRDGVILCKLANKLAPGSVKKIQERGTN-- 218
E W+ + LG LP E+ LR G++LC + N++ PG+V K+ E +
Sbjct: 59 EAARWVRNTLGVVGGRDLPADPSEEDFRIALRSGILLCNVLNRVKPGAVPKVVEAPNDPL 118
Query: 219 ----------FQLMENIQRFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 356
FQ EN++ F +++ G+P F+ +D + ++ C+ AL
Sbjct: 119 VNQDGAALSAFQYFENLRNFLVFVEEMGIP---TFEVSDFEKGGKSARIVECVLAL 171
>At3g44730.1 68416.m04814 kinesin motor protein-related similar to 4
other kinesin-like proteins of A. thaliana: F02P16.12
(PID:g2191180), katA (D11371), katB (D21137), and katC
(D21138); contains non-consensus AT-AC splice sites at
intron 10
Length = 1087
Score = 35.5 bits (78), Expect = 0.042
Identities = 19/39 (48%), Positives = 24/39 (61%), Gaps = 4/39 (10%)
Frame = +3
Query: 102 EPLPNGAYED----VLRDGVILCKLANKLAPGSVKKIQE 206
E LP ED LR+G+ILC + NK+ PGSV K+ E
Sbjct: 7 ETLPEKPSEDEFSLALRNGLILCNVLNKVNPGSVLKVVE 45
>At5g35700.1 68418.m04269 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893, fimbrin [Schizosaccharomyces pombe]
GI:3057144; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 687
Score = 34.7 bits (76), Expect = 0.073
Identities = 18/39 (46%), Positives = 25/39 (64%)
Frame = +3
Query: 102 EPLPNGAYEDVLRDGVILCKLANKLAPGSVKKIQERGTN 218
+P N A+ D+++DGV+LCKL N PG+ I ER N
Sbjct: 149 DPATN-AFFDLVKDGVLLCKLINVAVPGT---IDERAIN 183
Score = 29.9 bits (64), Expect = 2.1
Identities = 14/37 (37%), Positives = 24/37 (64%)
Frame = +3
Query: 81 WISDVLGEPLPNGAYEDVLRDGVILCKLANKLAPGSV 191
WI+ + N +ED LR+G +L ++ +K++PGSV
Sbjct: 401 WINSLGTATYVNNVFED-LRNGWVLLEVLDKVSPGSV 436
>At5g48460.1 68418.m05992 fimbrin-like protein, putative strong
similarity to fimbrin-like protein AtFim2 [Arabidopsis
thaliana] GI:2737926; contains Pfam profile PF00307:
Calponin homology (CH) domain
Length = 654
Score = 34.3 bits (75), Expect = 0.097
Identities = 20/44 (45%), Positives = 25/44 (56%)
Frame = +3
Query: 105 PLPNGAYEDVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN 236
P N +E V +DGV+LCKL N PG+ I ER N + M N
Sbjct: 152 PSSNDLFE-VAKDGVLLCKLINVAVPGT---IDERAINTKSMLN 191
Score = 31.9 bits (69), Expect = 0.52
Identities = 17/44 (38%), Positives = 25/44 (56%)
Frame = +3
Query: 60 QEQEVLTWISDVLGEPLPNGAYEDVLRDGVILCKLANKLAPGSV 191
+E+ WI+ G N +ED LRDG IL + +K++PG V
Sbjct: 396 EEKAFRFWINSFDGSVYINNVFED-LRDGWILLQTLDKVSPGIV 438
>At4g26700.1 68417.m03848 fimbrin-like protein (FIM1) identical to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893
Length = 687
Score = 33.9 bits (74), Expect = 0.13
Identities = 19/45 (42%), Positives = 27/45 (60%)
Frame = +3
Query: 102 EPLPNGAYEDVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN 236
+P N YE +++DGV+LCKL N PG+ I ER N + + N
Sbjct: 150 DPHSNQLYE-LVKDGVLLCKLINVAVPGT---IDERAINTKRVLN 190
>At5g55400.1 68418.m06902 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 714
Score = 33.5 bits (73), Expect = 0.17
Identities = 19/45 (42%), Positives = 27/45 (60%)
Frame = +3
Query: 102 EPLPNGAYEDVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN 236
+P N YE +++DGV+LCKL N PG+ I ER N + + N
Sbjct: 151 DPDSNDLYE-LVKDGVLLCKLINIAVPGT---IDERAINTKRVLN 191
Score = 31.1 bits (67), Expect = 0.90
Identities = 16/37 (43%), Positives = 24/37 (64%)
Frame = +3
Query: 81 WISDVLGEPLPNGAYEDVLRDGVILCKLANKLAPGSV 191
WI+ + E N +EDV R+G IL ++ +K+ PGSV
Sbjct: 402 WINSLGIESYVNNVFEDV-RNGWILLEVVDKVYPGSV 437
>At2g04750.1 68415.m00485 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 652
Score = 32.7 bits (71), Expect = 0.30
Identities = 17/38 (44%), Positives = 23/38 (60%)
Frame = +3
Query: 105 PLPNGAYEDVLRDGVILCKLANKLAPGSVKKIQERGTN 218
P N + D+++DGV+LCKL N PG+ I ER N
Sbjct: 144 PTTNALF-DLVKDGVLLCKLINIAVPGT---IDERAIN 177
>At5g62240.1 68418.m07815 expressed protein various predicted
proteins, Arabidopsis thaliana; expression supported by
MPSS
Length = 366
Score = 31.5 bits (68), Expect = 0.68
Identities = 19/55 (34%), Positives = 27/55 (49%)
Frame = +3
Query: 153 LCKLANKLAPGSVKKIQERGTNFQLMENIQRFQAAIKKYGVPEEEIFQTADLFER 317
L K +AP V K+Q + TN Q E ++ K VP+E +TA+ ER
Sbjct: 161 LAKFGENVAPVLVSKLQNQDTNRQKQEAKVAHVSSRAKLTVPKEPNLRTAERSER 215
>At5g25475.2 68418.m03031 expressed protein
Length = 282
Score = 31.1 bits (67), Expect = 0.90
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = -1
Query: 102 LPKHQRSK*EPLVLGPCFWQALCAGEIW 19
LPK+ K + V PCFW++L G+ W
Sbjct: 2 LPKNAIEKIQANVSKPCFWKSLSPGQTW 29
>At5g25475.1 68418.m03030 expressed protein
Length = 282
Score = 31.1 bits (67), Expect = 0.90
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = -1
Query: 102 LPKHQRSK*EPLVLGPCFWQALCAGEIW 19
LPK+ K + V PCFW++L G+ W
Sbjct: 2 LPKNAIEKIQANVSKPCFWKSLSPGQTW 29
>At3g59100.1 68416.m06589 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1934
Score = 31.1 bits (67), Expect = 0.90
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -3
Query: 187 DPGANLLANLQRITPSLSTSSYAPFGSGSPKTSEIQVRTSCSWSLF 50
D A+ N ITP + +G PKT+ ++VRT W+LF
Sbjct: 444 DLKADFFLNSDEITPQDERLNQVTYGKSKPKTNFVEVRT--FWNLF 487
>At3g11170.1 68416.m01355 omega-3 fatty acid desaturase, chloroplast
(FAD7) (FADD) identical to omega-3 fatty acid
desaturase, chloroplast precursor SP:P46310 [Arabidopsis
thaliana (Mouse-ear cress)]; identical to Pfam profile
PF00487: Fatty acid desaturase; identical to cDNA
plastid fatty acid desaturase GI:809491
Length = 446
Score = 29.9 bits (64), Expect = 2.1
Identities = 18/49 (36%), Positives = 28/49 (57%), Gaps = 2/49 (4%)
Frame = -3
Query: 193 FTDPGANLLANLQRITPSLSTSSYAPFGSGSPKTSEIQVRT--SCSWSL 53
+T P +N L+N + PSLS+SSY S SP + + R + +W+L
Sbjct: 18 YTTPRSNFLSNNNKFRPSLSSSSYKT--SSSPLSFGLNSRDGFTRNWAL 64
>At5g25470.2 68418.m03029 expressed protein
Length = 280
Score = 29.5 bits (63), Expect = 2.8
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -1
Query: 102 LPKHQRSK*EPLVLGPCFWQALCAGEIW 19
LP++ K + V PCFW++L G+ W
Sbjct: 2 LPRNATDKIQGNVSKPCFWKSLSPGQNW 29
>At5g25470.1 68418.m03028 expressed protein
Length = 280
Score = 29.5 bits (63), Expect = 2.8
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -1
Query: 102 LPKHQRSK*EPLVLGPCFWQALCAGEIW 19
LP++ K + V PCFW++L G+ W
Sbjct: 2 LPRNATDKIQGNVSKPCFWKSLSPGQNW 29
>At2g17010.1 68415.m01961 mechanosensitive ion channel
domain-containing protein / MS ion channel
domain-containing protein contains Pfam profile PF00924:
Mechanosensitive ion channel
Length = 779
Score = 29.5 bits (63), Expect = 2.8
Identities = 27/103 (26%), Positives = 47/103 (45%), Gaps = 3/103 (2%)
Frame = +3
Query: 15 ETRFRLH--TMPARNKDQEQEVLTWIS-DVLGEPLPNGAYEDVLRDGVILCKLANKLAPG 185
ETR R++ + A N + +++ +S L E + YED + K A A
Sbjct: 406 ETRSRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSEKEAKAAARK 465
Query: 186 SVKKIQERGTNFQLMENIQRFQAAIKKYGVPEEEIFQTADLFE 314
K +++RG + +E++ RF + E+E +T LFE
Sbjct: 466 IFKNVEQRGAKYIYLEDLMRF--------LREDEAMKTMGLFE 500
>At2g25730.1 68415.m03084 expressed protein
Length = 2464
Score = 29.1 bits (62), Expect = 3.7
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = -3
Query: 478 RLSSALWARSCSSVKVRSFLSAI 410
R + LW+RSC S + SFLS I
Sbjct: 52 RFDNVLWSRSCPSPSLLSFLSTI 74
>At3g07070.1 68416.m00840 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 414
Score = 28.7 bits (61), Expect = 4.8
Identities = 20/64 (31%), Positives = 28/64 (43%)
Frame = +3
Query: 48 RNKDQEQEVLTWISDVLGEPLPNGAYEDVLRDGVILCKLANKLAPGSVKKIQERGTNFQL 227
R KD EQ ++TW V EP D +GV K N+ + +QE T L
Sbjct: 287 RPKD-EQNLVTWAQPVFKEPSRFPELADPSLEGVFPEKALNQAVAVAAMCLQEEATVRPL 345
Query: 228 MENI 239
M ++
Sbjct: 346 MSDV 349
>At2g46510.1 68415.m05796 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 566
Score = 28.7 bits (61), Expect = 4.8
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +2
Query: 131 CAKGRCDPLQIGQQISSWISEKNPRERNQLPAHG 232
C + R L G I S + EK PR+R + PA+G
Sbjct: 356 CTEKRPVSLLAGAGIVSVVDEKRPRKRGRKPANG 389
>At2g31010.1 68415.m03781 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 775
Score = 28.7 bits (61), Expect = 4.8
Identities = 16/51 (31%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = +3
Query: 81 WISDVLGEPLPNGAYEDVLRDGVILCKLANKL-APGSVKKIQERGTNFQLM 230
W + +L EP+PNG Y V+ D + +L N+L P + + E G +++
Sbjct: 57 WDTGILSEPIPNGFY-SVVPDKRVK-ELYNRLPTPSELHALGEEGVRIEVI 105
>At2g45600.1 68415.m05670 expressed protein low similarity to PrMC3
[Pinus radiata] GI:5487873
Length = 329
Score = 28.3 bits (60), Expect = 6.4
Identities = 17/53 (32%), Positives = 26/53 (49%), Gaps = 3/53 (5%)
Frame = +3
Query: 12 LETRFRL---HTMPARNKDQEQEVLTWISDVLGEPLPNGAYEDVLRDGVILCK 161
L +RL H +PA +D + +L W+ D P+ G + L+DGV K
Sbjct: 103 LSVEYRLAPEHRLPAAYEDAVEAIL-WLRDQARGPINGGDCDTWLKDGVDFSK 154
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,489,329
Number of Sequences: 28952
Number of extensions: 428300
Number of successful extensions: 1231
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1228
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1843581600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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