BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30085X (441 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g18780.2 68416.m02386 actin 2 (ACT2) identical to SP|Q96292 A... 120 3e-28 At1g49240.1 68414.m05520 actin 8 (ACT8) identical to SP|Q96293 A... 120 3e-28 At5g09810.1 68418.m01135 actin 7 (ACT7) / actin 2 identical to S... 120 6e-28 At5g59370.1 68418.m07440 actin 4 (ACT4) identical to SP|P53494 A... 118 2e-27 At3g53750.1 68416.m05938 actin 3 (ACT3) identical to SP|P53493 A... 118 2e-27 At3g46520.1 68416.m05050 actin 12 (ACT12) identical to SP|P53497... 118 2e-27 At3g12110.1 68416.m01507 actin 11 (ACT11) identical to SP|P53496... 118 2e-27 At2g37620.1 68415.m04615 actin 1 (ACT1) identical to SP|P10671 A... 118 2e-27 At2g42170.1 68415.m05219 actin, putative similar to actin 2 [Ara... 103 7e-23 At2g42100.1 68415.m05205 actin, putative very strong similarity ... 102 1e-22 At2g42090.1 68415.m05204 actin, putative similar to SP|P53496 Ac... 94 3e-20 At3g18780.1 68416.m02385 actin 2 (ACT2) identical to SP|Q96292 A... 77 5e-15 At1g18450.1 68414.m02302 actin-related protein 4 (ARP4) neary id... 72 2e-13 At3g60830.1 68416.m06805 actin-related protein 7 (ARP7) identica... 59 2e-09 At3g33520.1 68416.m04291 actin-related protein 6 (ARP6) nearly i... 44 5e-05 At1g13180.1 68414.m01528 actin-related protein 3 (ARP3) identica... 43 8e-05 At3g27000.1 68416.m03378 actin-related protein 2 (ARP2) nearly i... 42 2e-04 At5g56180.1 68418.m07008 actin-related protein, putative (ARP8) ... 28 2.4 At5g43500.2 68418.m05318 expressed protein 27 7.4 At5g43500.1 68418.m05319 expressed protein 27 7.4 At5g07240.1 68418.m00826 calmodulin-binding family protein conta... 26 9.8 >At3g18780.2 68416.m02386 actin 2 (ACT2) identical to SP|Q96292 Actin 2 {Arabidopsis thaliana}; nearly identical to SP|Q96293 Actin 8 [Arabidopsis thaliana] GI:1669387 and to At1g49240 Length = 377 Score = 120 bits (290), Expect = 3e-28 Identities = 53/59 (89%), Positives = 58/59 (98%) Frame = -3 Query: 439 ITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 263 ITALAPS++KIK++APPERKYSVWIGGSILASLSTFQQMWISK EYDE+GPGIVHRKCF Sbjct: 319 ITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWISKAEYDEAGPGIVHRKCF 377 >At1g49240.1 68414.m05520 actin 8 (ACT8) identical to SP|Q96293 Actin 8 {Arabidopsis thaliana}; nearly identical to SP|Q96292 Actin 2 [Arabidopsis thaliana] GI:1669387, and to At3g18780 Length = 377 Score = 120 bits (290), Expect = 3e-28 Identities = 53/59 (89%), Positives = 58/59 (98%) Frame = -3 Query: 439 ITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 263 ITALAPS++KIK++APPERKYSVWIGGSILASLSTFQQMWISK EYDE+GPGIVHRKCF Sbjct: 319 ITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWISKAEYDEAGPGIVHRKCF 377 >At5g09810.1 68418.m01135 actin 7 (ACT7) / actin 2 identical to SP|P53492 Actin 7 (Actin-2) {Arabidopsis thaliana} Length = 377 Score = 120 bits (288), Expect = 6e-28 Identities = 53/59 (89%), Positives = 57/59 (96%) Frame = -3 Query: 439 ITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 263 ITALAPS++KIK++APPERKYSVWIGGSILASLSTFQQMWISK EYDESGP IVHRKCF Sbjct: 319 ITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWISKSEYDESGPSIVHRKCF 377 >At5g59370.1 68418.m07440 actin 4 (ACT4) identical to SP|P53494 Actin 4 {Arabidopsis thaliana} Length = 377 Score = 118 bits (284), Expect = 2e-27 Identities = 52/59 (88%), Positives = 57/59 (96%) Frame = -3 Query: 439 ITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 263 ITALAPS++KIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGP IVHRKCF Sbjct: 319 ITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 377 >At3g53750.1 68416.m05938 actin 3 (ACT3) identical to SP|P53493 Actin 3 {Arabidopsis thaliana}; supported by full-length cDNA: Ceres: 19581. Length = 377 Score = 118 bits (284), Expect = 2e-27 Identities = 52/59 (88%), Positives = 57/59 (96%) Frame = -3 Query: 439 ITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 263 ITALAPS++KIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGP IVHRKCF Sbjct: 319 ITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 377 >At3g46520.1 68416.m05050 actin 12 (ACT12) identical to SP|P53497 Actin 12 {Arabidopsis thaliana} Length = 377 Score = 118 bits (284), Expect = 2e-27 Identities = 52/59 (88%), Positives = 57/59 (96%) Frame = -3 Query: 439 ITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 263 ITALAPS++KIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGP IVHRKCF Sbjct: 319 ITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 377 >At3g12110.1 68416.m01507 actin 11 (ACT11) identical to SP|P53496 Actin 11 {Arabidopsis thaliana} Length = 377 Score = 118 bits (284), Expect = 2e-27 Identities = 52/59 (88%), Positives = 57/59 (96%) Frame = -3 Query: 439 ITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 263 ITALAPS++KIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGP IVHRKCF Sbjct: 319 ITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 377 >At2g37620.1 68415.m04615 actin 1 (ACT1) identical to SP|P10671 Actin 1 (Actin 3) {Arabidopsis thaliana} Length = 377 Score = 118 bits (284), Expect = 2e-27 Identities = 52/59 (88%), Positives = 57/59 (96%) Frame = -3 Query: 439 ITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 263 ITALAPS++KIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGP IVHRKCF Sbjct: 319 ITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 377 >At2g42170.1 68415.m05219 actin, putative similar to actin 2 [Arabidopsis thaliana] gi|9293903|dbj|BAB01806 Length = 329 Score = 103 bits (246), Expect = 7e-23 Identities = 44/59 (74%), Positives = 53/59 (89%) Frame = -3 Query: 439 ITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 263 I ALA + ++IKI+APPERKYSVWIGGSILASLST++QMWI+K EY+E+GP IVH KCF Sbjct: 271 INALAAANMRIKIVAPPERKYSVWIGGSILASLSTYEQMWITKAEYEENGPAIVHTKCF 329 >At2g42100.1 68415.m05205 actin, putative very strong similarity to SP|P53496 Actin 11 {Arabidopsis thaliana}, SP|P53493 Actin 3 {Arabidopsis thaliana}; contains Pfam profile PF00022: Actin Length = 378 Score = 102 bits (244), Expect = 1e-22 Identities = 44/59 (74%), Positives = 51/59 (86%) Frame = -3 Query: 439 ITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 263 I ALAP ++KIK++APPERKYSVW+GGSILASLS+F MWI+K EYDE G IVHRKCF Sbjct: 320 INALAPPSMKIKVVAPPERKYSVWVGGSILASLSSFAPMWITKAEYDEQGGAIVHRKCF 378 >At2g42090.1 68415.m05204 actin, putative similar to SP|P53496 Actin 11 {Arabidopsis thaliana}; contains Pfam profile PF00022: Actin Length = 366 Score = 94.3 bits (224), Expect = 3e-20 Identities = 39/58 (67%), Positives = 49/58 (84%) Frame = -3 Query: 439 ITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKC 266 + AL PS++K+K++ PPE + SVWIGGSILASLSTF QMWI+K+EY+E G IVHRKC Sbjct: 308 LNALVPSSMKVKVVVPPESECSVWIGGSILASLSTFHQMWITKDEYEEHGAAIVHRKC 365 >At3g18780.1 68416.m02385 actin 2 (ACT2) identical to SP|Q96292 Actin 2 {Arabidopsis thaliana}; nearly identical to SP|Q96293 Actin 8 [Arabidopsis thaliana] GI:1669387 and to At1g49240 Length = 371 Score = 77.0 bits (181), Expect = 5e-15 Identities = 35/43 (81%), Positives = 41/43 (95%) Frame = -3 Query: 439 ITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISK 311 ITALAPS++KIK++APPERKYSVWIGGSILASLSTFQQ+ I + Sbjct: 319 ITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQVKIDQ 361 >At1g18450.1 68414.m02302 actin-related protein 4 (ARP4) neary identical to actin-related protein 4 (ARP4) [Arabidopsis thaliana] GI:21427463; contains Pfam profile PF00022: Actin; supporting cDNA gi|21427462|gb|AF507912.1| Length = 441 Score = 72.1 bits (169), Expect = 2e-13 Identities = 31/57 (54%), Positives = 42/57 (73%), Gaps = 3/57 (5%) Frame = -3 Query: 427 APSTIKIKIIAP---PERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKC 266 +P + ++K++A ER++SVWIGGSILASL +FQQMW SK EY+E G + RKC Sbjct: 384 SPHSARVKVLASGNTTERRFSVWIGGSILASLGSFQQMWFSKSEYEEHGASYIQRKC 440 >At3g60830.1 68416.m06805 actin-related protein 7 (ARP7) identical to actin-related protein 7 (ARP7) [Arabidopsis thaliana] GI:21427469; contains Pfam profile PF00022: Actin Length = 363 Score = 58.8 bits (136), Expect = 2e-09 Identities = 27/62 (43%), Positives = 38/62 (61%), Gaps = 6/62 (9%) Frame = -3 Query: 430 LAPSTIKIKIIAPPERK------YSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRK 269 L S I+ ++ PPE YS W+GG+ILA + Q ++K +YDE+GP +VHRK Sbjct: 302 LCSSAIRPTLVKPPEYMPENLGMYSAWVGGAILAKVVFPQNQHVTKADYDETGPSVVHRK 361 Query: 268 CF 263 CF Sbjct: 362 CF 363 >At3g33520.1 68416.m04291 actin-related protein 6 (ARP6) nearly identical to actin-related protein 6 (ARP6) [Arabidopsis thaliana] GI:21427467; contains Pfam profile PF00022: Actin Length = 421 Score = 44.0 bits (99), Expect = 5e-05 Identities = 21/56 (37%), Positives = 29/56 (51%) Frame = -3 Query: 430 LAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 263 L P +KI + VW GGS+LAS F+ M ++K EY+E G R+ F Sbjct: 365 LVPDHFDVKITTQEDPILGVWRGGSLLASSPDFESMCVTKAEYEELGSARCRRRFF 420 >At1g13180.1 68414.m01528 actin-related protein 3 (ARP3) identical to actin-related protein 3 (ARP3) [Arabidopsis thaliana] GI:21427461; contains Pfam profile PF00022: Actin Length = 427 Score = 43.2 bits (97), Expect = 8e-05 Identities = 16/45 (35%), Positives = 30/45 (66%) Frame = -3 Query: 415 IKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGI 281 +++ +++ P ++++VW GGS+L+S F +KEEY+E G I Sbjct: 372 VEVNVVSHPVQRFAVWFGGSVLSSTPEFFASCRTKEEYEEYGASI 416 >At3g27000.1 68416.m03378 actin-related protein 2 (ARP2) nearly identical to actin-related protein 2 (ARP2) [Arabidopsis thaliana] GI:3818624; contains Pfam profile PF00022: Actin Length = 389 Score = 41.5 bits (93), Expect = 2e-04 Identities = 16/43 (37%), Positives = 30/43 (69%), Gaps = 1/43 (2%) Frame = -3 Query: 415 IKIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISKEEYDESG 290 ++++I PP RK+ V++GG++LA + + WI++E+Y E G Sbjct: 337 LRLRIEDPPRRKHMVYLGGAVLAGIMKDAPEFWINREDYMEEG 379 >At5g56180.1 68418.m07008 actin-related protein, putative (ARP8) strong similarity to actin-related protein 8A (ARP8) [Arabidopsis thaliana] GI:21427473; contains Pfam profile PF00022: Actin; supporting cDNA gi|21427470|gb|AF507916.1| Length = 471 Score = 28.3 bits (60), Expect = 2.4 Identities = 14/44 (31%), Positives = 27/44 (61%), Gaps = 3/44 (6%) Frame = -3 Query: 424 PSTIK--IKIIAPPERKYSVWIGGSILASLSTFQQMW-ISKEEY 302 PS+I I++I PP + W G ++++LS F W I+++++ Sbjct: 420 PSSISNGIRVIPPPYGVDTSWHGAKLISNLSIFPGPWCITRKQF 463 >At5g43500.2 68418.m05318 expressed protein Length = 584 Score = 26.6 bits (56), Expect = 7.4 Identities = 10/33 (30%), Positives = 19/33 (57%) Frame = -3 Query: 388 ERKYSVWIGGSILASLSTFQQMWISKEEYDESG 290 E ++ W GG+IL L ++ WI + ++ +G Sbjct: 527 EPQFVTWKGGAILGILDFGREAWIERHQWMVNG 559 >At5g43500.1 68418.m05319 expressed protein Length = 596 Score = 26.6 bits (56), Expect = 7.4 Identities = 10/33 (30%), Positives = 19/33 (57%) Frame = -3 Query: 388 ERKYSVWIGGSILASLSTFQQMWISKEEYDESG 290 E ++ W GG+IL L ++ WI + ++ +G Sbjct: 539 EPQFVTWKGGAILGILDFGREAWIERHQWMVNG 571 >At5g07240.1 68418.m00826 calmodulin-binding family protein contains Pfam profile PF00612: IQ calmodulin-binding motif Length = 401 Score = 26.2 bits (55), Expect = 9.8 Identities = 9/22 (40%), Positives = 13/22 (59%) Frame = -2 Query: 425 ALDHQDQDHRSPREEVLRMDRW 360 A+DH +HRSP +D+W Sbjct: 205 AMDHHHNNHRSPMGSSRLLDQW 226 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 7,924,385 Number of Sequences: 28952 Number of extensions: 145311 Number of successful extensions: 387 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 383 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 386 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 702840360 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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