Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30083
(919 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 235 6e-63
SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 226 3e-60
SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr... 175 1e-44
SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr... 168 8e-43
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 145 9e-36
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 30 0.40
SPCC1919.03c |||AMP-activated protein kinase beta subunit |Schiz... 27 2.8
SPBPB21E7.07 |aes1|SPAPB21E7.07, SPAPB21E7.07|enhancer of RNA-me... 27 3.7
SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharo... 27 4.9
SPAC23C4.02 |crn1||actin binding protein, coronin Crn1|Schizosac... 27 4.9
SPBC887.14c |pfh1|pif1|pif1 helicase homolog Pfh1|Schizosaccharo... 26 6.5
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 26 8.6
SPAC4G8.05 |ppk14||serine/threonine protein kinase Ppk14 |Schizo... 26 8.6
>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1526
Score = 235 bits (575), Expect = 6e-63
Identities = 118/239 (49%), Positives = 160/239 (66%), Gaps = 3/239 (1%)
Frame = +2
Query: 134 KPYDGKKACWVPDEKEGFVQGEIKATKGD---LVTVNLPGGETKDFKKDQVAQVNPPKYE 304
K D K+ W+ D + F + IK D +V N E K +D++ VNP K++
Sbjct: 19 KDIDDKRWVWISDPETAFTKAWIKEDLPDKKYVVRYNNSRDE-KIVGEDEIDPVNPAKFD 77
Query: 305 KCEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTTRCAKLYRGK 484
+ DM+ LTYLN+ +V YNL+QRY IYTYSGLF VA+NPY P+YT +LY+ K
Sbjct: 78 RVNDMAELTYLNEPAVTYNLEQRYLSDQIYTYSGLFLVAVNPYCGLPIYTKDIIQLYKDK 137
Query: 485 RRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKD 664
+ PH+FAI+D AY N+L N ENQS+L+TGESGAGKTENTK++I Y A + +S
Sbjct: 138 TQERKLPHVFAIADLAYNNLLENKENQSILVTGESGAGKTENTKRIIQYLAAIASSTTVG 197
Query: 665 PSQEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETY 841
SQ +E+Q+++TNPVLE+FGNA+TVRN+NSSRFGKFI++ F SG+++ A IE Y
Sbjct: 198 SSQ-----VEEQIIKTNPVLESFGNARTVRNNNSSRFGKFIKVEFSLSGEISNAAIEWY 251
Score = 39.5 bits (88), Expect = 7e-04
Identities = 14/25 (56%), Positives = 21/25 (84%)
Frame = +1
Query: 844 VEKARVISQQALERSYHIFYQMMSG 918
+EK+RV+ Q ER+YH+FYQ++SG
Sbjct: 253 LEKSRVVHQNEFERNYHVFYQLLSG 277
>SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2104
Score = 226 bits (553), Expect = 3e-60
Identities = 114/235 (48%), Positives = 150/235 (63%), Gaps = 1/235 (0%)
Frame = +2
Query: 140 YDGKKACWVPDEKEGFVQGEIKATKGDLVTVNLP-GGETKDFKKDQVAQVNPPKYEKCED 316
+D + W+PD KE FV+ I G+ V L G + +VNPPK++ +D
Sbjct: 34 FDERTWIWIPDSKESFVKAWIVEDLGEKYRVKLERDGSERIVDGFDAEKVNPPKFDMVDD 93
Query: 317 MSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTTRCAKLYRGKRRSE 496
M+ LT LN+ SV+ NL QRY LIYTYSGLF VA+NPY P+Y + Y+ K+ E
Sbjct: 94 MAALTCLNEPSVVNNLTQRYEKDLIYTYSGLFLVAVNPYCHLPIYGDDVVRKYQSKQFKE 153
Query: 497 VPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKDPSQE 676
PHIF +D AY ++L NQS+L+TGESGAGKTE TKKVI Y +V + D Q
Sbjct: 154 TKPHIFGTADAAYRSLLERRINQSILVTGESGAGKTETTKKVIQYLTSVTDASTSDSQQ- 212
Query: 677 KKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETY 841
LE ++++TNPVLEAFGNA+TVRN+NSSRFGKFIRI F +G + GA+++ Y
Sbjct: 213 ----LEKKILETNPVLEAFGNAQTVRNNNSSRFGKFIRIEFSNNGSIVGANLDWY 263
Score = 35.1 bits (77), Expect = 0.014
Identities = 13/25 (52%), Positives = 20/25 (80%)
Frame = +1
Query: 844 VEKARVISQQALERSYHIFYQMMSG 918
+EK+RVI + ER+YH+FYQ++ G
Sbjct: 265 LEKSRVIHPSSNERNYHVFYQLLRG 289
>SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1516
Score = 175 bits (425), Expect = 1e-44
Identities = 94/246 (38%), Positives = 148/246 (60%), Gaps = 18/246 (7%)
Frame = +2
Query: 158 CWVPDEKEGFVQGEIKATKGDLVTVNLPGGETKDFKKDQVAQVNPPKYE----------- 304
CW+PDE+ ++ G IK + + L + + + + + YE
Sbjct: 12 CWIPDEQSQWIPGSIKDCRVEGEKAFLTVQDENENETVITVKPDDLNYEGRNGLPFLRSI 71
Query: 305 --KCEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFP-VYTTRCAKLY 475
+D+++L+YLN+ SVL L RY IYTYSG+ +A+NP++R P +YT + Y
Sbjct: 72 NSDADDLTDLSYLNEPSVLDALSTRYNQLQIYTYSGIVLIAVNPFQRLPNLYTHEIVRAY 131
Query: 476 RGKRRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGAS- 652
K R E+ PH++AI++ +Y M H+NQ+++I+GESGAGKT + + ++ YFA+V A
Sbjct: 132 SEKSRDELDPHLYAIAEDSYKCMNQEHKNQTIIISGESGAGKTVSARYIMRYFASVQALI 191
Query: 653 QKKDPSQEKKGSL---EDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAG 823
Q D + + L E++++ TNP++EAFGN+KT RNDNSSRFGK+I+I F + + G
Sbjct: 192 QSTDSNFHEAPQLTAVENEILATNPIMEAFGNSKTSRNDNSSRFGKYIQILFDGNATIIG 251
Query: 824 ADIETY 841
A I+TY
Sbjct: 252 AKIQTY 257
Score = 33.9 bits (74), Expect = 0.032
Identities = 12/25 (48%), Positives = 21/25 (84%)
Frame = +1
Query: 844 VEKARVISQQALERSYHIFYQMMSG 918
+E++R++ Q ER+YHIFYQ+++G
Sbjct: 259 LERSRLVFQPNQERNYHIFYQILAG 283
>SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1471
Score = 168 bits (409), Expect = 8e-43
Identities = 93/234 (39%), Positives = 136/234 (58%), Gaps = 6/234 (2%)
Frame = +2
Query: 158 CWVPDEKEGFVQG---EIKATKGDLV--TVNLPGGETKDFKKDQVAQVNPPKYEKCEDMS 322
CWV + + EIK G V TV G + Q+ N + E D++
Sbjct: 12 CWVSNNNGHWDAARLIEIKDNGGGKVVATVAKSSGVLETVNYQQLQNRNIGQSESPSDLT 71
Query: 323 NLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFP-VYTTRCAKLYRGKRRSEV 499
NL YLN+ SVL+ L RY +K IYTYSG+ V+INPY+ P Y K + +
Sbjct: 72 NLPYLNEPSVLHALHNRYNNKQIYTYSGIVLVSINPYQNLPEFYNDNLIKHFHKDPEAAK 131
Query: 500 PPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKDPSQEK 679
PH+++I+ Y + T+ +NQ+++++GESGAGKT K ++ Y +V Q D +
Sbjct: 132 VPHLYSIASSCYHALTTDSKNQTIIVSGESGAGKTVAAKYIMRYLTSV---QGVDHNGVV 188
Query: 680 KGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETY 841
K S+E+QV+ TNP++EAFGNAKT+RNDNSSRFGK++ I F + + GA++ TY
Sbjct: 189 KRSVENQVLATNPIMEAFGNAKTIRNDNSSRFGKYVTISFDENLLITGANVNTY 242
Score = 33.9 bits (74), Expect = 0.032
Identities = 13/25 (52%), Positives = 21/25 (84%)
Frame = +1
Query: 844 VEKARVISQQALERSYHIFYQMMSG 918
+E++RV+S ER+YHIFYQ+++G
Sbjct: 244 LERSRVVSLLKGERNYHIFYQLITG 268
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 145 bits (351), Expect = 9e-36
Identities = 70/177 (39%), Positives = 110/177 (62%)
Frame = +2
Query: 311 EDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTTRCAKLYRGKRR 490
+D++ L+ + D + NL+ R+ + IYTY G +++NP++ +YT K Y+GK R
Sbjct: 43 DDLTLLSKITDEEINKNLELRFRNGEIYTYIGHVLISVNPFRDLGIYTMDILKSYQGKNR 102
Query: 491 SEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKDPS 670
E PH++AI++ AY M + HENQ ++I+GESGAGKTE K+++ Y V S +
Sbjct: 103 LETSPHVYAIAENAYYQMKSYHENQCIIISGESGAGKTEAAKRIMQYITHVSKSVGTEIE 162
Query: 671 QEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETY 841
+ + + ++ TNP+LE+FG AKT+RN+NSSR GK++ + F G GA I Y
Sbjct: 163 R-----VSEIILATNPLLESFGCAKTLRNNNSSRHGKYLEMIFNSGGVPVGAKITNY 214
Score = 31.1 bits (67), Expect = 0.23
Identities = 12/24 (50%), Positives = 18/24 (75%)
Frame = +1
Query: 835 NLSVEKARVISQQALERSYHIFYQ 906
N +EK R+++Q ER++HIFYQ
Sbjct: 213 NYLLEKNRIVNQVRNERNFHIFYQ 236
>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
Prp43|Schizosaccharomyces pombe|chr 2|||Manual
Length = 735
Score = 30.3 bits (65), Expect = 0.40
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +2
Query: 554 HENQSMLITGESGAGKTENTKKVIAY 631
HENQ ++ GE+G+GKT + + Y
Sbjct: 89 HENQIIVFVGETGSGKTTQIPQFVLY 114
>SPCC1919.03c |||AMP-activated protein kinase beta subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 298
Score = 27.5 bits (58), Expect = 2.8
Identities = 17/51 (33%), Positives = 23/51 (45%)
Frame = +2
Query: 290 PPKYEKCEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRF 442
PP EKC SN Y D SVL N + L + L +A++ R+
Sbjct: 233 PPHLEKCILNSNTAYKEDQSVLPNPNHVLLNHLAAANTQLGVLALSATTRY 283
>SPBPB21E7.07 |aes1|SPAPB21E7.07, SPAPB21E7.07|enhancer of
RNA-mediated gene silencing |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 296
Score = 27.1 bits (57), Expect = 3.7
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = -2
Query: 849 LNR*VSMSAPASFPDGPKWIRMNLPKREEL 760
LN +S P DGPKW+ + LP ++
Sbjct: 150 LNYSSQVSPPVLIDDGPKWLVIQLPNATDV 179
>SPAC3G6.01 |hrp3||ATP-dependent DNA helicase
Hrp3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1388
Score = 26.6 bits (56), Expect = 4.9
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = +2
Query: 554 HENQSMLITGESGAGKTENTKKVIAYFA 637
H+N++ ++ E G GKT T ++Y A
Sbjct: 391 HKNENGILADEMGLGKTVQTVAFLSYLA 418
>SPAC23C4.02 |crn1||actin binding protein, coronin
Crn1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 601
Score = 26.6 bits (56), Expect = 4.9
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = +2
Query: 638 TVGASQKKDPSQEKKGSLEDQVVQTNPVLEA 730
T KK+PS+EKK + D+ + P+ E+
Sbjct: 504 TFSEDVKKEPSEEKKLEVSDEAPKAAPLAES 534
>SPBC887.14c |pfh1|pif1|pif1 helicase homolog
Pfh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 805
Score = 26.2 bits (55), Expect = 6.5
Identities = 26/107 (24%), Positives = 47/107 (43%), Gaps = 3/107 (2%)
Frame = +2
Query: 314 DMSNLTYLNDASVLYNLKQRYYHKLIYT--YSGLFCVAINPYKRFPVYTTRCAKLYR-GK 484
D SN +S L +L ++ K T S F V +N + P+ ++ L++
Sbjct: 244 DSSNKLSNGRSSSLDSLAKKRMSKSKSTPQISKKFSVPLNSASKSPIGSS----LFKTSD 299
Query: 485 RRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVI 625
R + P IF + + + + S+ TG +G GK+ +K+I
Sbjct: 300 SRKKSVPSIFLSDEQKRILDMVVEQQHSIFFTGSAGTGKSVLLRKII 346
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 25.8 bits (54), Expect = 8.6
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = -2
Query: 93 YGVGSGSSPSWTTGFGIFGG 34
+G GSG P GFG FGG
Sbjct: 193 FGGGSGGPPPGPGGFGGFGG 212
>SPAC4G8.05 |ppk14||serine/threonine protein kinase Ppk14
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 566
Score = 25.8 bits (54), Expect = 8.6
Identities = 16/53 (30%), Positives = 27/53 (50%)
Frame = +2
Query: 662 DPSQEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLA 820
+ S+E + ++ED + + + G+ K S+F FIR+HF S K A
Sbjct: 8 ESSEEGRINVEDHLEEAKK--DDTGHWKHSGTAKPSKFRAFIRLHFKDSRKFA 58
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,746,374
Number of Sequences: 5004
Number of extensions: 79293
Number of successful extensions: 271
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 263
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 466510270
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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