BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30058 (547 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 79 4e-16 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 47 2e-06 SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 47 2e-06 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 40 3e-04 SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 33 0.036 SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p... 29 0.34 SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 29 0.34 SPBC14C8.01c |cut2|SPBC1815.02c|securin|Schizosaccharomyces pomb... 27 1.4 SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 27 1.8 SPAC110.02 |pds5||cohesin-associated protein Pds5|Schizosaccharo... 26 3.2 SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 26 4.2 SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 26 4.2 SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 26 4.2 SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 25 7.3 SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc... 25 7.3 SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual 25 7.3 SPBC1773.11c |mug89||CDC50 domain protein|Schizosaccharomyces po... 25 9.6 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 79.0 bits (186), Expect = 4e-16 Identities = 36/102 (35%), Positives = 63/102 (61%) Frame = -1 Query: 547 ELIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLR 368 ++I EVDAD +G ++F EF+T+ A+ + + D E +E+REAF+++DK+GNGYI L Sbjct: 52 DMINEVDADGNGTIDFTEFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELT 108 Query: 367 EIXXXXXXXXXXXXXXXLIQEIDTDGSGTVDFDEFMEMMTGE 242 + +I+E DTDG G ++++EF +++ + Sbjct: 109 HVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVISSK 150 Score = 50.0 bits (114), Expect = 2e-07 Identities = 23/62 (37%), Positives = 35/62 (56%) Frame = -1 Query: 436 ELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXLIQEIDTDGSGTVDFDEFME 257 E REAF L+D++ +G I ++ L + +I E+D DG+GT+DF EF+ Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72 Query: 256 MM 251 MM Sbjct: 73 MM 74 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 47.2 bits (107), Expect = 2e-06 Identities = 27/102 (26%), Positives = 48/102 (47%) Frame = -1 Query: 547 ELIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLR 368 +++ + D G L+ +FV + + IVE D +E++ AF L+D + G I +LR Sbjct: 77 KILRDFDKTGKGYLQMEDFVRVMTEKIVERDP---LEEIKRAFELFDDDETGKISLRNLR 133 Query: 367 EIXXXXXXXXXXXXXXXLIQEIDTDGSGTVDFDEFMEMMTGE 242 + +I+E D D G ++ EF+ +M E Sbjct: 134 RVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAIMMDE 175 Score = 33.9 bits (74), Expect = 0.016 Identities = 16/71 (22%), Positives = 33/71 (46%) Frame = -1 Query: 460 EDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXLIQEIDTDGSGT 281 E E ++++ EAF+L+D + + I LR ++++ D G G Sbjct: 30 EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89 Query: 280 VDFDEFMEMMT 248 + ++F+ +MT Sbjct: 90 LQMEDFVRVMT 100 >SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 174 Score = 46.8 bits (106), Expect = 2e-06 Identities = 29/105 (27%), Positives = 49/105 (46%), Gaps = 5/105 (4%) Frame = -1 Query: 544 LIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSL-- 371 L VD D G ++F EF+ + F V + E ++L+ AF++YD + +GYI L Sbjct: 61 LFSVVDEDGGGDVDFQEFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYL 117 Query: 370 ---REIXXXXXXXXXXXXXXXLIQEIDTDGSGTVDFDEFMEMMTG 245 + I E+D D G + F+EF ++++G Sbjct: 118 VLKMMVGTNLREDQLQQIVDKTIMEVDKDRDGKISFEEFKDIVSG 162 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 39.5 bits (88), Expect = 3e-04 Identities = 20/80 (25%), Positives = 42/80 (52%) Frame = -1 Query: 496 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXX 317 +F++ + + E ++E +E +AFR++DK+ +GYI T+ + Sbjct: 62 KFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118 Query: 316 LIQEIDTDGSGTVDFDEFME 257 ++QE D SG+ D+ +F++ Sbjct: 119 MVQEADPTNSGSFDYYDFVQ 138 Score = 29.1 bits (62), Expect = 0.45 Identities = 12/25 (48%), Positives = 18/25 (72%) Frame = -1 Query: 436 ELREAFRLYDKEGNGYIPTSSLREI 362 E++EAF LYD + +G IPTS + + Sbjct: 10 EMKEAFVLYDIDKDGLIPTSHVGSV 34 >SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 190 Score = 32.7 bits (71), Expect = 0.036 Identities = 20/56 (35%), Positives = 30/56 (53%) Frame = -1 Query: 529 DADKSGRLEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREI 362 DADK+G ++F EF+ + V E + +L AF+LYD + NG I + I Sbjct: 73 DADKNGYIDFKEFICALS---VTSRGE-LNDKLIWAFQLYDLDNNGLISYDEMLRI 124 >SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces pombe|chr 1|||Manual Length = 2280 Score = 29.5 bits (63), Expect = 0.34 Identities = 19/65 (29%), Positives = 31/65 (47%), Gaps = 1/65 (1%) Frame = +2 Query: 263 ELVEVDGAASVSV-DLLDEAIEFIVSQLLVQFPEDLAQARRGNVTVAFLVVQSERFPEFF 439 EL V A S DL D AI ++Q+L F +DL + VT+ + +P ++ Sbjct: 1246 ELTNVVNVALTSTGDLDDSAIVSKLNQILSDFRDDLLEHNVRRVTIVGGRINKSAYPSYY 1305 Query: 440 LHGLS 454 + +S Sbjct: 1306 TYRVS 1310 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 29.5 bits (63), Expect = 0.34 Identities = 15/60 (25%), Positives = 31/60 (51%) Frame = -1 Query: 430 REAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXLIQEIDTDGSGTVDFDEFMEMM 251 ++AF L+D+ G G IP +S+ ++ I EI++ VD ++F++++ Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDL----LRACGQNPTLAEITEIESTLPAEVDMEQFLQVL 64 >SPBC14C8.01c |cut2|SPBC1815.02c|securin|Schizosaccharomyces pombe|chr 2|||Manual Length = 301 Score = 27.5 bits (58), Expect = 1.4 Identities = 14/21 (66%), Positives = 15/21 (71%) Frame = +3 Query: 483 RVTNSGNSRRPDLSASTSSIS 545 RVT SGNSRR LS S S+S Sbjct: 267 RVTASGNSRRRPLSRSIHSLS 287 >SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 27.1 bits (57), Expect = 1.8 Identities = 11/18 (61%), Positives = 15/18 (83%) Frame = -1 Query: 439 KELREAFRLYDKEGNGYI 386 +EL+EAF L DK+G+G I Sbjct: 48 QELKEAFALLDKDGDGNI 65 >SPAC110.02 |pds5||cohesin-associated protein Pds5|Schizosaccharomyces pombe|chr 1|||Manual Length = 1205 Score = 26.2 bits (55), Expect = 3.2 Identities = 12/34 (35%), Positives = 20/34 (58%) Frame = +3 Query: 18 VDNIKYNSTTPDQLRTINYSLYNTYIILEKLADR 119 V+N++YN P +L T+ T +EK+AD+ Sbjct: 747 VENLEYNGGLPVRLMTLGQLFLYTLEEVEKVADQ 780 >SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 899 Score = 25.8 bits (54), Expect = 4.2 Identities = 10/19 (52%), Positives = 13/19 (68%) Frame = -1 Query: 547 ELIEEVDADKSGRLEFPEF 491 E ++ DAD SG+L F EF Sbjct: 337 ETFQKADADHSGKLSFEEF 355 >SPAC1687.14c |||EF hand family protein, unknown role|Schizosaccharomyces pombe|chr 1|||Manual Length = 76 Score = 25.8 bits (54), Expect = 4.2 Identities = 12/26 (46%), Positives = 14/26 (53%) Frame = -1 Query: 445 MQKELREAFRLYDKEGNGYIPTSSLR 368 M +E EAF L+D GYI LR Sbjct: 10 MDEEAEEAFDLFDVTHKGYIDFEDLR 35 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 25.8 bits (54), Expect = 4.2 Identities = 15/36 (41%), Positives = 21/36 (58%) Frame = -2 Query: 369 ARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSS 262 A ++G+ TSS T + SS S T +A +STSS Sbjct: 968 ASATGSSTSSIATASVSGSSTSSVATASATDSSTSS 1003 Score = 25.4 bits (53), Expect = 5.5 Identities = 16/38 (42%), Positives = 21/38 (55%) Frame = -2 Query: 375 ACARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSS 262 A A +G+ TSS T + SS S T +A +STSS Sbjct: 1005 AAASVTGSSTSSVATASVTDSSTSSVATASATDSSTSS 1042 >SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Manual Length = 614 Score = 25.0 bits (52), Expect = 7.3 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = -1 Query: 547 ELIEEVDADKSGRLEFPEFV 488 E I EV+ D SGR+E +FV Sbjct: 58 EAIREVNVDSSGRVEPEDFV 77 >SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 830 Score = 25.0 bits (52), Expect = 7.3 Identities = 15/34 (44%), Positives = 20/34 (58%) Frame = -1 Query: 499 PEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEG 398 PEF AA+ +VEED E + EA + D+EG Sbjct: 38 PEFSDEAAEALVEEDIEDLD---GEALDVEDEEG 68 >SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual Length = 341 Score = 25.0 bits (52), Expect = 7.3 Identities = 12/40 (30%), Positives = 17/40 (42%) Frame = -2 Query: 378 RACARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSSW 259 R C S +W SS +T + S+R L S + W Sbjct: 59 RLCKLPSKSWVSSTVTNEKLRESRRLALQAYVQCLSETPW 98 >SPBC1773.11c |mug89||CDC50 domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 396 Score = 24.6 bits (51), Expect = 9.6 Identities = 15/44 (34%), Positives = 24/44 (54%) Frame = +3 Query: 15 SVDNIKYNSTTPDQLRTINYSLYNTYIILEKLADRYCFVAAN*C 146 +V + KY ++ DQ TI + +TY LEK+ D+ + N C Sbjct: 92 AVPSKKYTASF-DQSGTIGFDKESTYWKLEKILDKDLDMDVNYC 134 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,802,571 Number of Sequences: 5004 Number of extensions: 30494 Number of successful extensions: 134 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 108 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 127 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 225926624 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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