BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30051 (688 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 263 1e-71 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 134 9e-33 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 121 1e-28 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 116 3e-27 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 93 3e-20 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 77 2e-15 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 72 7e-14 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 70 3e-13 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 42 6e-05 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.5 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 2.5 SPBC2D10.03c |||DUF866 domain protein|Schizosaccharomyces pombe|... 25 7.8 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 263 bits (645), Expect = 1e-71 Identities = 120/136 (88%), Positives = 128/136 (94%) Frame = -2 Query: 687 YELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANPVM 508 YELPDGQVITIGNERFR PEALFQPS LG+E+ GIHE YNSIMKCDVDIRKDLY N VM Sbjct: 240 YELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVM 299 Query: 507 SGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISK 328 SGGTTMYPGIADRMQKEI ALAPS++K+KI+APPERKYSVWIGGSILASLSTFQQMWISK Sbjct: 300 SGGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISK 359 Query: 327 EEYDESGPGIVHRKCF 280 +EYDESGPGIV+RKCF Sbjct: 360 QEYDESGPGIVYRKCF 375 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 134 bits (325), Expect = 9e-33 Identities = 60/136 (44%), Positives = 92/136 (67%) Frame = -2 Query: 687 YELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANPVM 508 Y+LPD Q IT+G E F PE LF P +G E+ G+H ++ SI+ D+D+R LY+N V+ Sbjct: 245 YQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVL 304 Query: 507 SGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISK 328 SGG+T+ G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ Sbjct: 305 SGGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITS 364 Query: 327 EEYDESGPGIVHRKCF 280 EEY ++ ++ R+ F Sbjct: 365 EEY-KNDQNVIFRRRF 379 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 121 bits (291), Expect = 1e-28 Identities = 61/139 (43%), Positives = 91/139 (65%), Gaps = 12/139 (8%) Frame = -2 Query: 687 YELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANPVM 508 Y LPDG+VI +G+ER+ CPE LFQP +G E G+ E ++++I DVDIRK LY V+ Sbjct: 240 YTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVL 299 Query: 507 SGGTTMYPGIADRMQKEITAL--------APSTI---KIKIIAPPERKYSVWIGGSILAS 361 SGG++MY G+ R++KEI L P+ + K+KI P R+++V+IGG++LA Sbjct: 300 SGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLAD 359 Query: 360 -LSTFQQMWISKEEYDESG 307 ++ MW+SK E++E G Sbjct: 360 IMAQNDHMWVSKAEWEEYG 378 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 116 bits (279), Expect = 3e-27 Identities = 61/138 (44%), Positives = 80/138 (57%), Gaps = 11/138 (7%) Frame = -2 Query: 687 YELPDGQVITIGNERFRCPEALFQPSFLGMESC---------GIHETVYNSIMKCDVDIR 535 +E PDG G ERFR E LF PSF S G+HE VY SI+ CD ++R Sbjct: 281 FEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELR 340 Query: 534 KDLYANPVMSGGTTMYPGIADRMQKEITALAP-STIKIKII-APPERKYSVWIGGSILAS 361 L N V++GGT++ PG+++R+Q E+ LA S I + +VW GGSILAS Sbjct: 341 SPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILAS 400 Query: 360 LSTFQQMWISKEEYDESG 307 L FQ +W+SK+EYDE G Sbjct: 401 LDNFQHLWVSKQEYDEVG 418 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 93.1 bits (221), Expect = 3e-20 Identities = 46/108 (42%), Positives = 70/108 (64%), Gaps = 6/108 (5%) Frame = -2 Query: 588 GIHETVYNSIMKCDVDIRKDLYANPVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 409 GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384 Query: 408 P---ERKYSVWIGGSILASLSTFQQMWISKEEYDESGP---GIVHRKC 283 ER Y+ W+GGSIL+SL TF Q+WIS++EY+E G ++ ++C Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 77.0 bits (181), Expect = 2e-15 Identities = 43/128 (33%), Positives = 64/128 (50%) Frame = -2 Query: 669 QVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANPVMSGGTTM 490 QV+ + NERF PE LF PS + + GI E V S+ +I+ L N V GG Sbjct: 272 QVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCK 331 Query: 489 YPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDES 310 +PG R+ E+ +LAP+ ++K+ P + W S + L + I++ EY E Sbjct: 332 FPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEH 390 Query: 309 GPGIVHRK 286 G I+ RK Sbjct: 391 GANIMTRK 398 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 72.1 bits (169), Expect = 7e-14 Identities = 41/140 (29%), Positives = 67/140 (47%), Gaps = 15/140 (10%) Frame = -2 Query: 663 ITIGNERFRCPEALFQPSFLGMESCG-IHETVYNSIMKCDVDIRKDLYANPVMSGGTTMY 487 I +G ERF PE F P + + E V N + +D+RK LY N V+SGG+T++ Sbjct: 280 IDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLF 339 Query: 486 PGIADRMQKEITALAPSTI--------------KIKIIAPPERKYSVWIGGSILASLSTF 349 +R+Q+++ + I + +I+ ++ +VW GGS+LA F Sbjct: 340 KNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEF 399 Query: 348 QQMWISKEEYDESGPGIVHR 289 +K +Y+E G I R Sbjct: 400 GSYCHTKADYEEYGASIARR 419 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 70.1 bits (164), Expect = 3e-13 Identities = 32/115 (27%), Positives = 62/115 (53%) Frame = -2 Query: 648 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANPVMSGGTTMYPGIADR 469 E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 468 MQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGP 304 +++E+T++ P I + W G S + F+ +++EEY E GP Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGP 708 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 42.3 bits (95), Expect = 6e-05 Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%) Frame = -2 Query: 534 KDLYANPVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILA 364 K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586 Query: 363 SLSTFQQMWISKEEYDESGPGIVHRK 286 + ++WI ++ G ++ K Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 1.5 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 3/37 (8%) Frame = -2 Query: 387 WIGGSILASLSTFQQM---WISKEEYDESGPGIVHRK 286 ++GGSI+A S + + +++ EEY + GP +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 2.5 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = -2 Query: 462 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 352 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPBC2D10.03c |||DUF866 domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 157 Score = 25.4 bits (53), Expect = 7.8 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = +2 Query: 224 ELGRAQHHTAPGSRGRAA*KHLRWT 298 E+ R++ H+ PGS+G A +L WT Sbjct: 46 EISRSETHSIPGSKGEA---NLIWT 67 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,417,237 Number of Sequences: 5004 Number of extensions: 46216 Number of successful extensions: 127 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 115 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 120 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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