BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30044X (564 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 208 3e-55 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 100 2e-22 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 95 8e-21 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 94 1e-20 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 79 3e-16 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 61 1e-10 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 55 8e-09 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 53 3e-08 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 43 3e-05 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.1 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 1.9 SPBC2D10.03c |||DUF866 domain protein|Schizosaccharomyces pombe|... 25 5.8 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 25 7.7 SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 25 7.7 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 208 bits (509), Expect = 3e-55 Identities = 94/109 (86%), Positives = 102/109 (93%) Frame = -1 Query: 564 LGMESCGIHETVXNSIMKCDVDIRKDLYANAVMSGGTTMYPGIADRMQKEITALAPSTIK 385 LG+E+ GIHE NSIMKCDVDIRKDLY N VMSGGTTMYPGIADRMQKEI ALAPS++K Sbjct: 267 LGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMK 326 Query: 384 IKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 238 +KI+APPERKYSVWIGGSILASLSTFQQMWISK+EYDESGPGIV+RKCF Sbjct: 327 VKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVYRKCF 375 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 100 bits (239), Expect = 2e-22 Identities = 45/109 (41%), Positives = 74/109 (67%) Frame = -1 Query: 564 LGMESCGIHETVXNSIMKCDVDIRKDLYANAVMSGGTTMYPGIADRMQKEITALAPSTIK 385 +G E+ G+H + SI+ D+D+R LY+N V+SGG+T+ G +R E+ A++ + Sbjct: 272 MGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKNQ 331 Query: 384 IKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 238 +KI A PER ++ W+GGSILASLSTF+++ I+ EEY ++ ++ R+ F Sbjct: 332 VKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVIFRRRF 379 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 94.7 bits (225), Expect = 8e-21 Identities = 45/98 (45%), Positives = 63/98 (64%), Gaps = 2/98 (2%) Frame = -1 Query: 552 SCGIHETVXNSIMKCDVDIRKDLYANAVMSGGTTMYPGIADRMQKEITALAP-STIKIKI 376 S G+HE V SI+ CD ++R L N V++GGT++ PG+++R+Q E+ LA S I + Sbjct: 321 SVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHT 380 Query: 375 I-APPERKYSVWIGGSILASLSTFQQMWISKEEYDESG 265 +VW GGSILASL FQ +W+SK+EYDE G Sbjct: 381 AETASATSNAVWFGGSILASLDNFQHLWVSKQEYDEVG 418 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 93.9 bits (223), Expect = 1e-20 Identities = 46/108 (42%), Positives = 70/108 (64%), Gaps = 6/108 (5%) Frame = -1 Query: 546 GIHETVXNSIMKCDVDIRKDLYANAVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 367 GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384 Query: 366 P---ERKYSVWIGGSILASLSTFQQMWISKEEYDESGP---GIVHRKC 241 ER Y+ W+GGSIL+SL TF Q+WIS++EY+E G ++ ++C Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 79.4 bits (187), Expect = 3e-16 Identities = 44/112 (39%), Positives = 69/112 (61%), Gaps = 12/112 (10%) Frame = -1 Query: 564 LGMESCGIHETVXNSIMKCDVDIRKDLYANAVMSGGTTMYPGIADRMQKEITAL------ 403 +G E G+ E + ++I DVDIRK LY V+SGG++MY G+ R++KEI L Sbjct: 267 VGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVL 326 Query: 402 --APSTI---KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISKEEYDESG 265 P+ + K+KI P R+++V+IGG++LA ++ MW+SK E++E G Sbjct: 327 HGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWEEYG 378 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 61.3 bits (142), Expect = 1e-10 Identities = 32/111 (28%), Positives = 55/111 (49%), Gaps = 14/111 (12%) Frame = -1 Query: 537 ETVXNSIMKCDVDIRKDLYANAVMSGGTTMYPGIADRMQKEITALAPSTI---------- 388 E V N + +D+RK LY N V+SGG+T++ +R+Q+++ + I Sbjct: 309 ELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAK 368 Query: 387 ----KIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHR 247 + +I+ ++ +VW GGS+LA F +K +Y+E G I R Sbjct: 369 SGGVDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 54.8 bits (126), Expect = 8e-09 Identities = 25/101 (24%), Positives = 53/101 (52%) Frame = -1 Query: 564 LGMESCGIHETVXNSIMKCDVDIRKDLYANAVMSGGTTMYPGIADRMQKEITALAPSTIK 385 +G++ GI E + + + + ++ ++ L +N +++GG PG+ R+++E+T++ P Sbjct: 608 VGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETRIKRELTSIMPVGSS 667 Query: 384 IKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGP 262 I + W G S + F+ +++EEY E GP Sbjct: 668 INVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGP 708 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 52.8 bits (121), Expect = 3e-08 Identities = 31/105 (29%), Positives = 49/105 (46%) Frame = -1 Query: 558 MESCGIHETVXNSIMKCDVDIRKDLYANAVMSGGTTMYPGIADRMQKEITALAPSTIKIK 379 + GI E V S+ +I+ L N V GG +PG R+ E+ +LAP+ ++K Sbjct: 295 IREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKRLSSELRSLAPANWEVK 354 Query: 378 IIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRK 244 + P + W S + L + I++ EY E G I+ RK Sbjct: 355 VFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEHGANIMTRK 398 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 43.2 bits (97), Expect = 3e-05 Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%) Frame = -1 Query: 492 KDLYANAVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILA 322 K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586 Query: 321 SLSTFQQMWISKEEYDESGPGIVHRK 244 + ++WI ++ G ++ K Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 1.1 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 3/37 (8%) Frame = -1 Query: 345 WIGGSILASLSTFQQM---WISKEEYDESGPGIVHRK 244 ++GGSI+A S + + +++ EEY + GP +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 1.9 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = -1 Query: 420 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 310 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPBC2D10.03c |||DUF866 domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 157 Score = 25.4 bits (53), Expect = 5.8 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = +2 Query: 182 ELGRAQHHTAPGSRGRAA*KHLRWT 256 E+ R++ H+ PGS+G A +L WT Sbjct: 46 EISRSETHSIPGSKGEA---NLIWT 67 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 25.0 bits (52), Expect = 7.7 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = -3 Query: 394 DHQDQDHRSPREEVLRMDRWIHPG 323 D D+ R+ E VLRM R++ PG Sbjct: 499 DIDDKKDRALSEHVLRMHRYLPPG 522 >SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 981 Score = 25.0 bits (52), Expect = 7.7 Identities = 19/58 (32%), Positives = 25/58 (43%), Gaps = 2/58 (3%) Frame = -2 Query: 191 VRVLNATVSTLYLVIP--EN*TSNLTPSILM*FIVKFYINLISFLFLLYTRDRCGSQR 24 VR +A S +L P N SN TP+ + I K N+ S + L D C R Sbjct: 575 VRFFHAIQSHFHLEGPIRYNMNSNCTPNSIASIIQKKSYNVSSITYELLNEDACALSR 632 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,929,026 Number of Sequences: 5004 Number of extensions: 34735 Number of successful extensions: 92 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 83 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 87 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 238029836 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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